Transfection Carrier DNA from Promega

Product Specs
ItemTransfection Carrier DNA
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE4881
Quantity5 x 20microg
Molecular Weight~73kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) ApoFLARE: a luminescent reporter for direct quantification of APOBEC3A editing activitybioRxivMarch 17, 2026Maria Vittoria Di Marco, Braeden L. Butler, Christopher T. Eggers, Aaron N. Hataeither with or without 10-fold dilution of the reporter DNA into Transfection Carrier DNA (Promega, #E4881) to yield either 50ng or 5ng reporter per well in 5μl transfection mixture, and incubated for 24 hours

(2) Design and Development of DNA Damage Chemical Inducers of Proximity (DD-CIP) for Targeted Cancer TherapybioRxivNovember 6, 2025Tian Qiu, Yeuan Ting Lee, Brendan G. Dwyer, Yi Jer Tan, Ting Chen, Bryan A. Romero, Yanlan Wang, Jiehui Deng, Tinghu Zhang, Gerald R. Crabtree, et al.1.15 μg PARP1-Nanoluc fusion vector (Promega # CS3489A07), 10 μg Transfection Carrier DNA (Promega # E4881), 32 μL FuGENE HD transfection reagent (Promega #E2311) and 1 mL Opti-MEM. The cells were directly plated

(3) Deep mutational scanning of rabies glycoprotein defines mutational constraint and antibody-escape mutationsCell Host & MicrobeJune 11, 2025Arjun K. Aditham, Caelan E. Radford, Caleb R. Carr, Naveen Jasti, Neil P. King, Jesse D. Bloomrabies G expression plasmid, supplemented with 1.5μg of transfection carrier DNA (Promega, Ref. No. E4881). These plasmids were combined with 200μL of DMEM and 3μL of BioT transfection reagent, and we let this

(4) Protein binding in an mRNA 5′-UTR sterically hinders translationRNAFebruary 1, 2025Simon Felder, Irma M. Nelson, Breanne M. Hatfield, Kevin M. Weeksplasmid was firefly pGL4.50 vector (E1310, Promega), and contained transfection carrier plasmid DNA (E4881, Promega). All plasmids were transformed in DH5α Escherichia coli cells (C2987, NEB), grown in LB medium

(5) Sall4 and Gata4 induce cardiac fibroblast transition towards a partially multipotent state with cardiogenic potentialScientific ReportsOctober 15, 2024Hong Gao, Saliha Pathan, Beverly R. E. A. Dixon, Aarthi Pugazenthi, Megumi Mathison, Tamer M.A. Mohamed, Todd K. Rosengart, Jianchang Yang293T cell transfection procedures were previously described21. Transfection Carrier DNA (Promega, cat#E4881) was used as a mock treatment and for balancing total DNA amount. Promoter activity was measured using

(6) Multi-tiered chemical proteomic maps of tryptoline acrylamide–protein interactions in cancer cellsNature ChemistryOctober 1, 2024Evert Njomen, Rachel E. Hayward, Kristen E. DeMeester, Daisuke Ogasawara, Melissa M. Dix, Tracey Nguyen, Paige Ashby, Gabriel M. Simon, Stuart L. Schreiber, Bruno Melillo, et al.prepared in 1 mL of serum free Opti-MEM at a ratio of 9 μg/mL of Transfection Carrier DNA (Promega, E4881) and 1 μg/mL of LIMK1-NanoLuc plasmid. FuGENE HD reagent (30 μL) was then added to the DNA mixture,

(7) Lysine deserts and cullin-RING ligase receptors: Navigating untrodden paths in proteostasisiScienceNovember 17, 2023Natalia A. Szulc, Małgorzata Piechota, Lilla Biriczová, Pankaj Thapa, Wojciech Pokrzywa2.5 ng HiBiT-tagged VHL, SOCS1, ODC or 0.05 ng BRD4 expression constructs, diluted in carrier DNA (E4881, Promega) to obtain a final DNA amount of 50 ng/well. Transfection was carried out using the FuGENE HD Transfection Reagent

(8) REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein BiosensorsBiosensors (Basel)July 28, 2023Katrijn Boon, Nathan Vanalken, Eef Meyen, Dominique Schols, Tom Van Loyto the ratio used in the NanoBRET assay) was serially diluted (1:3) into Transfection carrier DNA (#E4881; Promega). As a negative control, only Transfection carrier DNA (#E4881; Promega) was combined with

(9) Development of a Covalent Inhibitor of c-Jun N-Terminal Protein Kinase (JNK) 2/3 with Selectivity over JNK1Journal of Medicinal ChemistryMarch 9, 2023Wenchao Lu, Yao Liu, Yang Gao, Qixiang Geng, Deepak Gurbani, Lianbo Li, Scott B. Ficarro, Cynthia J. Meyer, Dhiraj Sinha, Inchul You, et al.PIKFYVE-NanoLuc fusion vector (Promega, catalog no. NV4041) together with 4.5 μg of carrier DNA vector (Promega, catalog no. E4881) by reverse transfection. After transfection for 24 h, cells were resuspended at a density of 2.5 ×

(10) SGC-CLK-1: A chemical probe for the Cdc2-like kinases CLK1, CLK2, and CLK4Curr Res Chem BiolJanuary 1, 2023Tiek D, Wells CI, Schröder M, Song X, Alamillo-Ferrer C, Goenka A, Iglesia R, Lu M, Hu B, Kwarcinski F, et al.(ATCC CRL1573) in growth media (DMEM + 10% FBS) was added 525 μL of a 9:1 ratio of carrier DNA (Promega E4881) to a CLK nLuc construct-CLK1 CLK2 or CLK4 (Promega – NV1131, NV1141, NV1151) in OPTImem (Gibco) + 15.75μL

(11) Complete mapping of mutations to the SARS-CoV-2 spike receptor-binding domain that escape antibody recognitionbioRxivJuly 3, 2022Allison J. Greaney et al.were transfected with 1 μg HDM_Spike_RBD_B7–1 plasmids and 1 μg of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci, B01–02), according to manufacturer’s protocol. At 18 to 20 h post-transfection,

(12) Attenuated influenza virions expressing the SARS-CoV-2 receptor-binding domain induce neutralizing antibodies in micebioRxivApril 2, 2022Andrea N. Loes, Lauren E. Gentles, Allison J. Greaney, Katharine H. D. Crawford, Jesse D. BloomHDM-SARS-Spike-delta21 or HDM_Spike_RBD_B7–1 plasmids and 1.5 ng of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci, B01–02), according to manufacturer’s protocol. Alternatively, media

(13) CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibitionNatureApril 1, 2022Gallo D, Young JTF, Fourtounis J, Martino G, Álvarez-Quilón A, Bernier C, Duffy NM, Papp R, Roulston A, Stocco R, et al.vector for PKMYT1 (Promega NV1871) or WEE1 (Promega NV2231) with transfection carrier DNA (Promega E4881) using Fugene HD Transfection reagent (Promega E2311) in Opti-MEM without phenol red (Thermo Fisher Scientific

(14) A kinetic intra-cellular assay (KICA) to measure quantitative compound binding kinetics within living cellsSTAR ProtocolsMarch 18, 2022Charles S. Lay, Daniel A. Thomas, John P. Evans, Emma J. Jones, Kelly M. Gatfield, Peter D. CraggsDNA ,, NL-BRD4,Promega Corporation,Cat. # N169A Transfection Carrier DNA,Promega Corporation,Cat. # E4881 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism v.

(15) A human coronavirus evolves antigenically to escape antibody immunityPLOS PathogensApril 8, 2021Rachel T. Eguia, Katharine H. D. Crawford, Terry Stevens-Ayers, Laurel Kelnhofer-Millevolte, Alexander L. Greninger, Janet A. Englund, Michael J. Boeckh, Jesse D. Bloomμg APN-expressing plasmid, 1 μg of TMPRSS2-expressing plasmid, and 0.5 μg of carrier DNA (Promega, E4881) to achieve an 8.5:1 ratio of APN:TMPRSS2. We found that this ratio gave sufficient TMPRSS2 expression, while

(16) Complete Mapping of Mutations to the SARS-CoV-2 Spike Receptor-Binding Domain that Escape Antibody RecognitionCell Host & MicrobeJanuary 13, 2021Allison J. Greaney et al.were transfected with 1 μg HDM_Spike_RBD_B7-1 plasmids and 1 μg of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci, B01-02), according to manufacturer’s protocol. At 18 to 20 h post-transfection,

(17) Attenuated Influenza Virions Expressing the SARS-CoV-2 Receptor-Binding Domain Induce Neutralizing Antibodies in MiceVirusesSeptember 5, 2020Andrea N. Loes, Lauren E. Gentles, Allison J. Greaney, Katharine H. D. Crawford, Jesse D. BloomHDM-SARS-Spike-delta21 or HDM_Spike_RBD_B7-1 plasmids and 1.5 ng of Transfection Carrier DNA (Promega, Madison, WI, E4881) using BioT reagent, according to manufacturer’s protocol. Alternatively, the media was changed to IGM

(18) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization AssaysVirusesMay 6, 2020Crawford KHD, Eguia R, Dingens AS, Loes AN, Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy M, et al.SARS-CoV-2 Spike (NR-52513, NR-52514, or NR-52515), VSV G (positive control), or transfection carrier DNA (E4881, Promega, Madison, WI, USA) as a negative control.At 18 to 24 h post-transfection, change the media

(19) High-Throughput Implementation of the NanoBRET Target Engagement Intracellular Kinase Assay to Reveal Differential Compound Engagement by SIK2/3 IsoformsSLAS DiscoveryFebruary 1, 2020Hyun Yong Jin, Yanyan Tudor, Kaylee Choi, Zhifei Shao, Brian A. Sparling, Joseph G. McGivern, Antony Symonsmedia. At the same time, cells were transfected with a mixture of 9 µg of promoter-less carrier DNA (#E4881, Promega, Madison, WI) and 1 µg of NanoLuc-SNF1LK2 Fusion vector (#NV2061, Promega) or NanoLuc-SIK3 Fusion vector

(20) Leveraging Compound Promiscuity to Identify Targetable Cysteines within the KinomeCell chemical biologyJune 20, 2019Rao S, Gurbani D, Du G, Everley RA, Browne CM, Chaikuad A, Tan L, Schröder M, Gondi S, Ficarro SB, et al.E2311 "PP384 well plate, white, flat bottom",Greiner BioOne,Cat# 781207 Transfection Carrier DNA,Promega,Cat# E4881 energy transfer probe 5,Promega,Cat# N2500 Tracer dilution buffer,Promega,Cat# N2150 NanoBRET NanoGlo

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