pNL1.1.TK[Nluc/TK] Vector from Promega

Product Specs
ItempNL1.1.TK[Nluc/TK] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1501
Quantity20microg
Nucleic Acid TypeReporter Vectors
Expression System (standardized)Mammalian
Vector NamepNL1.1.TK[Nluc/TK]

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37Cell ReportsApril 1, 2026Joana R. Loureiro, Ana F. Castro, Ana S. Figueiredo, Ana Eufrásio, Ashutosh Dhingra, Mafalda Galhardo, Hugo Marcelino, Catarina C. Rodrigues, Paula Sampaio, Maria Azevedo, et al.pGL3-basic/GW. These pGL3-basic luciferase reporter test vectors were co-transfected with pNL1.1TK[Nluc/TK (#N1501; Promega), in HEK293T cells. The activity of these sequences was assessed 24 h post-transfection, by

(2) The HIV-1 nuclear export complex reveals the role of RNA in CRM1 cargo recognitionMolecular CellAugust 21, 2025Amber M. Smith, Yang Li, Arianna Velarde, Yifan Cheng, Alan D. Frankelplasmid (pCMV gagpol-IRES FFL-RRE) and 0.5 ng of the reference plasmid pNL1.1.TK [Nluc/TK] (Promega, N1501) using PolyJet (SignaGen) following the manufacturer’s instructions. All assays were performed in biological

(3) Sequential activation of transcription factors promotes liver regeneration through specific and developmental enhancersCell GenomicsJuly 9, 2025Palmira Llorens-Giralt, Marina Ruiz-Romero, Ramil Nurtdinov, Macarena Herranz-Itúrbide, Guillermo P. Vicent, Florenci Serras, Isabel Fabregat, Montserrat Corominasreporter of interest per well (recombinant pGL4.24 [luc2P/minP/Hygro]) and 5 ng/mL of NanoLuc plasmid (N1501, Promega, Madison, WI, USA) in complete media. 16 h post-transfection, the medium was replaced, and

(4) Enhanced NF-κB activation via HIV-1 Tat-TRAF6 cross-talkScience AdvancesJanuary 19, 2024Yang Li, Xi Liu, Koh Fujinaga, John D. Gross, Alan D. Frankel(pGL4.32 [luc2P/NF-κB-RE/Hygro] Vector, E8491) and the control plasmid (pNL1.1.TK [Nluc/TK] Vector, N1501) were purchased from Promega. UBE2N and Uev1a were synthesized as a gBlock gene fragment (IDT) with

(5) Dual-specificity RNA aptamers enable manipulation of target-specific O-GlcNAcylation and unveil functions of O-GlcNAc on β-cateninCellJanuary 19, 2023Zhu Y, Hart GW.Firefly Luciferase (TOPFlash or FOPFlash)85 and the NanoLuc Luciferase (pNL1.1.TK[Nluc/TK], Promega N1501) were transfected into cells. For OGT knockdown and rescue assays, plasmids encoding shOGT and codon-optimized

(6) Protocol for a Wnt reporter assay to measure its activity in human neural stem cells derived from induced pluripotent stem cellsCurr Res NeurobiolJanuary 1, 2023Yde Ohki CM, Walter NM, Rickli M, Salazar Campos JM, Werling AM, Döring C, Walitza S, Grünblatt E.internal control vector under the control of a TK promoter (pNL1.1.TK [Nluc/TK] Vector; Promega AG, N1501) (Fig. 1B) were separately amplified in TOP10 E. coli (Life Technologies, C4040-03) using heat-shock

(7) Novel Cell Adhesion/Migration Pathways Are Predictive Markers of HDAC Inhibitor Resistance in Cutaneous T Cell LymphomaSSRN Electronic JournalApril 1, 2022Jared M. Andrews, Jennifer A. Schmidt, Kenneth R. Carson, Amy C. Musiek, Neha Mehta-Shah, Jacqueline Paytonconﬁrmed successful cloning. 1 μg of each luciferase plasmid and 15 ng of Promega's pNL1·1·TK vector (#N1501) were conucleofected into 1 × 106 HUT78 cells with an Amaxa Nucleofector 2b system using the X-001 program

(8) Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sortingCell reportsMarch 15, 2022Lewis AE, Kuwahara A, Franzosi J, Bush JO.plasmids were constructed using pNL1.1.PGK[Nluc/PGK] (Promega, N1441) and pNL1.1.TK[Nluc/TK] (Promega, N1501) plasmids; these plasmids contain PGK or TK constitutively active promoters, selected to assure a basal

(9) Nuclear-localized human respiratory syncytial virus NS1 protein modulates host gene transcriptionCell reportsOctober 12, 2021Pei J, Beri NR, Zou AJ, Hubel P, Dorando HK, Bergant V, Andrews RD, Pan J, Andrews JM, Sheehan KCF, et al.(pGL4.45),Promega,Cat#E4141 minP reporter (pGL4.23),Promega,Cat#E8411 Control luciferase reporter (pNL1.1.TK),Promega,Cat#N1501 ,, Software and algorithms,, ,, ImageJ,NIH,https://imagej.nih.gov/ij/ ZEN imaging software,ZEISS,https://www.zeiss.com/microscopy/int/products/microscope-software.html Prism

(10) Novel cell adhesion/migration pathways are predictive markers of HDAC inhibitor resistance in cutaneous T cell lymphomaEBioMedicineAugust 1, 2019Jared M. Andrews, Jennifer A. Schmidt, Kenneth R. Carson, Amy C. Musiek, Neha Mehta-Shah, Jacqueline E. Paytonconfirmed successful cloning. 1 μg of each luciferase plasmid and 15 ng of Promega's pNL1·1·TK vector (#N1501) were co-nucleofected into 1 × 106 HUT78 cells with an Amaxa Nucleofector 2b system using the X-001

(11) Schlafen12 Reduces the Aggressiveness of Triple Negative Breast Cancer through Post-Transcriptional Regulation of ZEB1 That Drives Stem Cell DifferentiationCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2019Al-Marsoummi S, Vomhof-DeKrey E, Basson MD.promotor proE-cad670Luc vector (Addgene, #42083) and 400ng/well of pNL1.1.TK[Nluc/TK] vector (Promega, #N1501). Seventy-two hours later, cells were lysed using Glo lysis buffer (Promega, #E2661) and 100μl of lysate

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