Fig 1: Comparative PCR analyses of different samples with the self-made enzyme and the commercial ones. PCR analyses of the same DNA fragment as a part of a plasmid (A) and bacterial chromosome (B). The reactions contained (1) 2.5 u and (2) 5 u of Taq DNA polymerase supplied by Evrogen; (3) 0.5 µl, (4) 1 µl and (5) 2 µl of the self-made enzyme. Lane M contains the DNA ladder (New England Biolabs, #N3239S): 0.5, 1, 1.5, 2, 3 (more intense fragment), 4, 5, 6, 8, 10, 15, 20, 48.5 kbp. A common master mix was prepared for all samples. (C) PCR analyses of two cassettes incorporated in a binary plasmid DNA (3, 6) and in genomic DNA of transgenic chrysanthemum plant (2, 5). Lane M contains the DNA ladder (Thermo Fisher Scientific, #SM0321): 100, 200, 300, 400, 500 (more intense fragment), 600, 700, 800, 900, 1000 (more intense fragment), 1200, 1500, 2000, 3000 bp. On the left from lane M there are the reactions which contained 1 u of Thermo Fisher Scientific Taq DNA polymerase (#EP0402). On the right from the lane M there are the reactions which contained 1 µl of the self-made enzyme. Lanes (1 and 4) contains the control reactions without addition of DNA. A common master mix was prepared for all samples.