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Molecular Biology
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pNL3.2[NlucP/minP] Vector
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pNL3.2[NlucP/minP] Vector
pNL3.2[NlucP/minP] Vector
pNL3.2[NlucP/minP] Vector from Promega
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Citations:
(6)
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pNL3.2[NlucP/minP] Vector
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Description
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL3.1[Nluc/minP] and pNL3.2[NlucP/minP] Vectors offer a minimal promoter for cloning response elements of interest
Product Specs
Company Info
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Reviews
Product Specs
Item
pNL3.2[NlucP/minP] Vector
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
N1041
Quantity
20microg
Vector Name
pNL3.2[NlucP/minP]
Nucleic Acid Type
Reporter Vectors
Expression System (standardized)
Mammalian
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (6)
(1)
Defining the Functional Role and Potential as an Immunotherapeutic Target of ALCAM in Neuroblastoma
Mol Cancer Ther
November 7, 2025
Jarrett Lindsay, Jonathan Gaither, David Groff, Minu Samanta, Rebecca Kaufman, Liron Grossmann, Nicholas Hartnett, Nathan Kendsersky, Emily A. Mycek, Sharon J. Diskin,
et al.
backbones encoding PEST-destabilized NanoLuciferase [pNL2.2 (NlucP/Hygro), #
N1071
; pNL3.2 (NlucP/minP), #
N1041
] or constitutive promoter–driven (TK) Firefly Luciferase control [pGL4.54 (luc2/TK), #
E5061
] were obtained
(2)
Defining the functional role and potential as an immunotherapeutic target of ALCAM in neuroblastoma
Molecular Cancer Therapeutics
November 7, 2025
Jarrett Lindsay, Jonathan Gaither, David Groff, Minu Samanta, Rebecca Kaufman, Liron Grossmann, Nicholas Hartnett, Nathan Kendsersky, Emily A. Mycek, Sharon J. Diskin,
et al.
backbones encoding PEST-destabilized NanoLuciferase (pNL2.2 [NlucP/Hygro], #
N1071
; pNL3.2 [NlucP/minP], #
N1041
) or constitutive-promoter-driven (TK) Firefly Luciferase control (pGL4.54 [luc2/TK], #
E5061
) were obtained
(3)
Nitroxoline mitigates hepatic steatosis by enhancing cholesterol efflux and promoting bile acid synthesis through LRH-1 signaling
Lipids in Health and Disease
September 29, 2025
Wen-Cheng Liu, Chih-Feng Lien, Yi-Jhen Huang, Pei-Yu Lien, Sy-Jou Chen, Chin-Sheng Lin, Rou-Ling Cho, Yi-Ping Chuang
Eugene (OR), USA) with 200 ng of the 5 × LRH-1 response element reporter plasmid (PNL3.2, Cat. No.
N1041
,
Promega
, Madison, WI, USA), along with 1 ng of pGL4.53 (Cat. No.
E5011
,
Promega
, Madison (WI), USA)
(4)
AR loss in prostate cancer stroma mediated by NF-κB and p38-MAPK signaling disrupts stromal morphogen production
Oncogene
June 28, 2024
Shekha Tahsin, Neha S. Sane, Cernyar Cernyar, Linan Jiang, Yitshak Yitshak, Benjamin R. Lee, Cindy K. Miranti
spanning −1040 to +499, was subcloned into the Nano luciferase reporter vector pNL3.2 [NlucP/minP] (cat
N1041
;
Promega
) using the
KpnI
and
BglII
(Gene Universal). AR promoter deletions were created by Gene Universal.
(5)
Whole-genome analysis of noncoding genetic variations identifies multiscale regulatory element perturbations associated with Hirschsprung disease
Genome research
November 1, 2020
Fu AX, Lui KN, Tang CS, Ng RK, Lai FP, Lau ST, Li Z, Garcia-Barcelo MM, Sham PC, Tam PK,
et al
.
intron 10 fragment was cloned into a NanoLuc luciferase reporter construct (pNL3.2[NlucP/minP]) (
Promega
N1041
) to generate the PIK3C2B-pNL construct, while
NFIA
ORF was cloned into the pFLAG-CMV plasmid to generate
(6)
Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements Facilitates Analysis of Biological Endpoint Effects of Cellular Ca
2+
Mobilization
International journal of molecular sciences
February 18, 2018
Zhang W, Takahara T, Achiha T, Shibata H, Maki M.
containing three NFAT-RE repeats into the upstream multiple
cloning
site of
pNL3.2[NlucP/minP] vector
(#
N1041
,
Promega
) as follows: (i) insertion of a pair of oligonucleotides (50 -ctgagctcgctagcttgaggaatttccattgaggaatttccattgaggaatttccatta-30 and
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pNL3.2[NlucP/minP] Vector from Promega
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