pNL3.1[Nluc/minP] Vector from Promega

Product Specs
ItempNL3.1[Nluc/minP] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1031
Quantity20microg
Nucleic Acid TypeReporter Vectors
Expression System (standardized)Mammalian
Vector NamepNL3.1[Nluc/minP]

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Protocol to quantify site-specific carbohydrate sulfation at immune checkpoint proteins in mammalian cellsSTAR ProtocJune 20, 2025Wen-Cheng Chou, Wei-Ting Chen, Yuh-Shan Jou, Chen-Yang Shen232650 "pLAS2w.Phyg-CHST8(3A) (R258A, E259A, P260A)",Chou et al.1,Addgene# 232651 pNL3.1,Promega,Cat #N1031 pXJ,This paper,N/A pXJ-Nanoluc (Nluc),Chou et al.1,Addgene# 232656 pXJ-Nluc-α(PLRSKK)CA1-19,Chou

(2) Biologically targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett’s esophagus and esophageal adenocarcinomaHuman Genetics and Genomics AdvancesApril 1, 2025Li Yan, Qianchuan He, Shiv P. Verma, Xu Zhang, Ann-Sophie Giel, Carlo Maj, Kathryn Graz, Elnaz Naderi, Jianhong Chen, Mourad Wagdy Ali, et al.Twist Biosciences and cloned into the minimal-promoter-NanoLuc reporter vector pNL3.1 (Promega, no. N1031) between KpnI and HindIII restriction sites in both orientations using In-fusion Snap Assembly Master

(3) A new pipeline SPICE identifies novel JUN-IKZF1 composite elementsElifeJanuary 9, 2025Peng Li, Sree H. Pulugulla, Sonali Das, Jangsuk Oh, Rosanne Spolski, Jian-Xin Lin, Warren J. Leonardwith LPS (1 μg ml–1) for 24 hr. 5×106 cells were electroporated with 1 μg of NanoLuc pNL3.1 (Promega, N1031) vector and 0.2 μg of control vector, pGL4.S4 luc TK (Promega) using the P3 Primary cell 4D-nucleofector

(4) Case-Case Genome-Wide Analyses Identify Subtype-Informative Variants That Confer Risk for Breast CancerCancer ResearchAugust 1, 2024Xiaohui Sun, Shiv Prakash Verma, Guochong Jia, Xinjun Wang, Jie Ping, Xingyi Guo, Xiao-Ou Shu, Jianhong Chen, Andriy Derkach, Qiuyin Cai, et al.by Twist Biosciences and cloned into the minimal-promoter-NanoLuc reporter vector pNL3.1 (Promega #N1031) between KpnI and HindIII restriction sites in both orientations using In-fusion Snap Assembly Master

(5) Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axisCell reportsApril 5, 2022Wang D, Tanaka-Yano M, Meader E, Kinney MA, Morris V, Lummertz da Rocha E, Liu N, Liu T, Zhu Q, Orkin SH, et al.double-stranded oligonucleotides containing Erg enhancer sequences were inserted into the pNL3.1 Vector (#N1031, Promega, USA) to establish the pNL3.1-Erg vector. Stable cell lines K562 (ATCC) cells were transfected

(6) Mutations in the transcription factor FOXO1 mimic positive selection signals to promote germinal center B cell expansion and lymphomagenesisImmunityAugust 10, 2021Roberto MP, Varano G, Vinas-Castells R, Holmes AB, Kumar R, Pasqualucci L, Farinha P, Scott DW, Dominguez-Sola D.3x DBE tandem via Gibson assembly into pNL3.1, which encodes for Nanoluc (Nluc) luciferase (Promega #N1031) driven by a minimal promoter. We also generated control, mutated constructs based containing a 3x tandem

(7) Neutralizing antibody against SARS-CoV-2 spike in COVID-19 patients, health care workers, and convalescent plasma donorsJCI insightNovember 19, 2020Zeng C, Evans JP, Pearson R, Qu P, Zheng YM, Robinson RT, Hall-Stoodley L, Yount J, Pannu S, Mallampalli RK, et al.NR-52281). Creation of a secreted intron-bearing Nano-Luc lentiviral vector. The secNluc construct (Promega, N1031, gift from Walther Mothes, Yale University, New Haven, Connecticut, USA) contains the Nluc construct

(8) Identification of potential regulatory mutations using multi-omics analysis and haplotyping of lung adenocarcinoma cell linesScientific ReportsDecember 1, 2018Sarun Sereewattanawoot, Ayako Suzuki, Masahide Seki, Yoshitaka Sakamoto, Takashi Kohno, Sumio Sugano, Katsuya Tsuchihara, Yutaka Suzukiour 137 regulatory SNVs fall within region specified by the databases. Luciferase assay pNL3.1 (#N1031, Promega) was selected as vector and pGL4.53 (#E5011, Promega) as control. Mutant and Wildtype fragment

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