Human Genome-Wide CRISPRa Dual-sgRNA Library (plasmid) from Cellecta

Pooled library of ~105,000 dual-sgRNA constructs each targeting a single gene. Designed to work with deactivated spCas9 (dCas9) complexed with an activator (e.g., dCas9-VPH) to activate gene expression. Five CRISPRa dual-sgRNA constructs target each gene. Designs based on Horlbeck, et al., eLife 2016;5:e19760 DOI: 10.7554)