pAAV-EF1a-Cre (AAV Retrograde) from Addgene Inc.

Product Specs
ItempAAV-EF1a-Cre (AAV Retrograde)
CompanyAddgene Inc.
Price Supplier Page View Company Product Page
Catalog Number55636-AAVrg
Source/Expression SystemMammalian
Molecule NameCre
Quantity100 µL
Puritysuitable purity for injection into animals
Speciesrecombinant adeno-associated virus (rAAV)
Vector NamepAAV-EF1a-Cre
PromoterEF1a

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Addgene Inc.

490 Arsenal Way

Suite 100

Watertown, Massachusetts 02472

United States

Phone: +1 (617) 233-4363

Email: help@addgene.org

Company Profile

Website: www.addgene.org/

(1) Cell type-specific barcoding reveals the single-neuron projectional architecture of the mouse midbrain dopaminergic systembioRxivApril 4, 2026Hyopil Kim, Cheng Xu, Craig Washington, Caleb Shi, Maggie Lowman, Justus M. Kebschullstandard curve. Additionally, we purchased retrograde AAV (AAVrg-EF1a-Cre) from Addgene (Addgene, #55636-AAVrg). Virus injections We anesthetized mice with isoflurane (4% for induction, 1–1.5% for maintenance)

(2) Distinct roles of prefrontal subregion feedback to the primary visual cortex across behavioral statesNeuronFebruary 4, 2026Sofie Ährlund-Richter, Yuma Osako, Kyle R. Jenks, Emma Odom, Haoyang Huang, Don B. Arnold, Mriganka SurVisual Cortex (AP: −3.5, ML: −2.5, DV: −0.3) and two adjacent injections of 0.2μl retroAAV-Ef1a-Cre (55636-AAVrg, Addgene, 7×1012 vg/mL) were injected at a rate of 0.05μl/min. After the retraction of the injection

(3) Prelimbic cortex to ventral tegmental area projection regulates early social isolation stress-potentiated heroin seeking in miceNat CommunOctober 29, 2025Yunwanbin Wang, Shuwen Yue, Fengwei Yang, Lu Chen, Archana Singh, Magnus Marciniak, Wei Wei, Zi-Jun Wangnanoliters of a retrograde pAAV-hSyn-DIO-hM3Dq-mCherry (Addgene, #44361-AAVrg) or AAVrg-Cre (Addgene, #55636-AAVrg) were bilaterally injected into the VTA (AP: −3.4, ML: ±0.46, DV: −4.2) (Fig. 2B). Injections were performed

(4) Basal forebrain cholinergic input mediates adaptive attention allocation to enhance olfactory discriminationPLOS BiologySeptember 16, 2025Rahul Garg, Qiang Qiu, C. Ron Yu(Addgene, 44362-AAVrg) in ChAT-Cre mice) or HDB fibers projecting to the OB (AAVrg-EF1a-Cre (Addgene, 55636-AAVrg) together with AAV2-DIO-hM4D(Gi)-mCherry in C57BL/6 mice). Excitatory DREADDs were bilaterally expressed

(5) Projecting neurons from the lateral entorhinal cortex to the basolateral amygdala mediate the encoding of incidental odor–taste associationsProceedings of the National Academy of SciencesJune 10, 2025Jose Antonio González-Parra, Vittoria Acciai, Laura Vidal-Palencia, Marc Canela-Grimau, Arnau Busquets-Garcia4:1 of retrograde pAAV-EF1a-Cre and retro pAAV-CAG-GFP viral vectors in the BLA (Addgene viral prep #55636-AAVrg with a titer of ≥7 × 1,012 vg/mL and viral prep #37825-AAVrg with a titer of ≥7 × 1,012 vg/mL and with

(6) Cadherins orchestrate specific patterns of perisomatic inhibition onto distinct pyramidal cell populationsNat CommunMay 14, 2025Julie Jézéquel, Giuseppe Condomitti, Tim Kroon, Fursham Hamid, Stella Sanalidou, Teresa Garcés, Patricia Maeso, Maddalena Balia, Zhaohui Hu, Setsuko Sahara, et al.access L5 IT, while L5 ET were specifically targeted by injecting 300 nl of rAAV2retro-Cre (AddGene #55636-AAVrg, 2.1013 vg/ml) in the Pons. The stereotaxic coordinates for the different target areas were the same

(7) Ketamine rescues anhedonia by cell-type- and input-specific adaptations in the nucleus accumbensNeuronMay 7, 2025Federica Lucantonio, Jacob Roeglin, Shuwen Li, Jaden Lu, Aleesha Shi, Katherine Czerpaniak, Francesca R. Fiocchi, Leonardo Bontempi, Brenda C. Shields, Carlos A. Zarate, et al.#50455-AAV8 AAV-EF1a-DIO-EYFP,Deisseroth lab,Addgene: #27056-AAV1 pAAVretro-EF1a-Cre,Fenno et al. 79,Addgene: #55636-AAVrg AAV1-CamKIIa-hChR2(H134R)-eyfp,Lee et al. 80,Addgene: #26969-AAV1 AAV-1/2-hSyn1-chl-dlox-eGFP-PA-Rac1(rev)-dlox-WPRE-bGHp(A),Custom

(8) Liver-innervating vagal sensory neurons are indispensable for the development of hepatic steatosis and anxiety-like behavior in diet-induced obese miceNat CommunJanuary 24, 2025Jiyeon Hwang, Sangbhin Lee, Junichi Okada, Li Liu, Jeffrey E. Pessin, Streamson C. Chua, Gary J. Schwartz, Young-Hwan Jodissolving it in a mixture of 90% corn oil and 10% ethanol. Viral injection We used AAVrg-Cre (Addgene 55636-AAVrg, titer; 2.2 × 1013 pfu/ml), AAVrg-FLEX-GFP (Addgene 51502-AAVrg, titer; 2.3 × 1013 pfu/ml), AAVrg-FLEX-jGCamp7s

(9) Kappa opioids inhibit spinal output neurons to suppress itchScience AdvancesSeptember 27, 2024Tayler D. Sheahan, Charles A. Warwick, Abby Y. Cui, David A. A. Baranger, Vijay J. Perry, Kelly M. Smith, Allison P. Manalo, Eileen K. Nguyen, H. Richard Koerber, Sarah E. Ross(Addgene, #50459-AAV2), AAV2-hsyn-DIO-hM3Dq-mCherry (Addgene, #44361-AAV2), AAVr-Ef1A-Cre (Addgene, #55636-AAVrg), and AAVr-hSyn-eGFP-Cre (Addgene, #105540-AAVrg). Viruses were delivered into the spinal cord or lPBN

(10) Prefrontal Cortex subregions provide distinct visual and behavioral feedback modulation to the Primary Visual CortexbioRxivAugust 15, 2024S. Ährlund-Richter, Y. Osako, K.R. Jenks, E. Odom, H. Huang, D.B. Arnold, M. SurVisual Cortex (AP: −3.5, ML: −2.5, DV: −0.3) and two adjacent injections of 0.2μl retroAAV-Ef1a-Cre (55636-AAVrg, Addgene, 7×1012 vg/mL) were injected at a rate of 0.05μl/min. After the retraction of the injection

(11) The functional and anatomical characterization of three spinal output pathways of the anterolateral tractCell reportsMarch 26, 2024Chen H, Bleimeister IH, Nguyen EK, Li J, Cui AY, Stratton HJ, Smith KM, Baccei ML, Ross SE.pENN.AAVrg-hSyn.HI-eGFP-Cre-WPRE.SV40(Addgene#105540),AAV1.hSyn.HI.eGFP-Cre.WPRE.SV40(Addgene#105540),AAVrg-EF1a-Cre (Addgne#55636), AAVrg-EF1a-Flpo (Addgne#100187), pENN.AAVrg-hSyn-Cre-WPRE.hGH (Addgene#105553), AAV8-hSyn-EGFP (Addgene# 50465), AAV8

(12) Disynaptic inhibitory cerebellar control over caudal medial accessory oliveeNeuroJanuary 19, 2024Willem S. van Hoogstraten, Marit C. C. Lute, Zhiqiang Liu, Robin Broersen, Luca Mangili, Lieke Kros, Zhenyu Gao, Xiaolu Wang, Arn M. J. M. van den Maagdenberg, Chris I. De Zeeuw(MDJ) tracing experiment, we injected a retrograde Cre virus AAVrg-pAAV-EF1a-Cre (titer >1 × 1012, No 55636-AAVrg; Addgene) in IO and a Cre-dependent virus pAAV9-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (titer

(13) Identification of a convergent spinal neuron population that encodes itchbioRxivNovember 12, 2023Tayler D. Sheahan, Charles A. Warwick, Abby Y. Cui, David A.A. Baranger, Vijay J. Perry, Kelly M. Smith, Allison P. Manalo, Eileen K. Nguyen, H. Richard Koerber, Sarah E. Rossstrains AAV2-hSyn-DIO-mCherry,Addgene,50459-AAV2 AAV2-hSyn-DIO-hM3Dq-mCherry,Addgene,44361-AAV2 AAVr-Ef1A-Cre,Addgene,55636-AAVrg AAVr.hSyn.HI.eGFP-Cre.WPRE.SV40,Addgene,105540-AAVrg "Chemicals, peptides, and recombinant proteins","Chemicals,

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pAAV-EF1a-Cre (AAV Retrograde) from Addgene Inc.

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Addgene Inc.

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pAAV-EF1a-Cre (AAV Retrograde) from Addgene Inc.

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