Fig 1: HuR bound to KLF9 mRNA to promote the mRNA stability. A, RNA pull-down assay was performed to test the binding between HuR and KLF9 mRNA (N = 3). B, The interaction between HuR and KLF9 mRNA was confirmed by RIP assay (N = 3). C, 293T cells were cotransfected with luciferase reporter plasmids carrying the 3′ UTR of KLF9 mRNA, a control shRNA (shNC), or the shHuR. After 24 h, cells were lysed, and luciferase activities were measured. The p values were calculated with Tukey’s test (N = 3). D, E, The effect of HuR knockdown on the mRNA stability of GAPDH and KLF9 was determined by qRT-PCR after actinomycin D treatment (N = 3). The p values were calculated with Tukey’s test. **p < 0.01, ##p < 0.01.