Western Blot analysis of cell lysates from HEK293T cells with/without transfection of SARS-CoV-2 N-Gene ORF clone(Cat# [VC102555]). Left: WB using antibody against N-protein (Cat# [TA890226]); Right: WB using antibody against C-terminal DDK tag (Cat# [TA150078]).
SARS-CoV-2 N Gene Tagged ORF Clone
Western blot validation of overexpression lysate (Cat# LY490001) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with VC102555 using transfection reagent MegaTran 2.0 (Cat# TT210002)
Left: HEK293T transfected with plasmids overexpressing SARS-Cov2 N-Gene (Cat# [VC102555]). Cells were fixed in 2% neutral buffer formalin and detected with N-protein antibody (Cat# [TA890226]). Polink1 anti Rabbit HRP polymer (Cat# [D13-18]) was done for 30 min room temperature followed by DAB. 40x image. Right: Untransfected HEK293T.
Screening for N-protein in paraffin-embedded on left mock transfected HEK 293T cells negative and on the right HEK 293T transfected with Myc-DDK-tagged ORF clone (Cat# VC102555) for SARS-CoV-2 nucleocapsid phosphoprotein showing positive stain. Immuno-cytochemical staining was done with rabbit polyclonal anti-SARS-CoV-2 N Protein (CAT# TA890226) at 1:200. No heat-induced epitope retrieval was used.
Supplier Page from OriGene Technologies for SARS-CoV-2 N Gene Tagged ORF Clone