pAAV-EF1a-Flpo (AAV1) from Addgene Inc.

Product Specs
ItempAAV-EF1a-Flpo (AAV1)
CompanyAddgene Inc.
Price Supplier Page View Company Product Page
Catalog Number55637-AAV1
Quantity100 µL
Puritysuitable purity for injection into animals
Speciesrecombinant adeno-associated virus (rAAV)
Molecule NameFlpo
Source/Expression SystemMammalian
Vector NamepAAV-EF1a-Flpo
PromoterEF1a

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Addgene Inc.

490 Arsenal Way

Suite 100

Watertown, Massachusetts 02472

United States

Phone: +1 (617) 233-4363

Email: help@addgene.org

Company Profile

Website: www.addgene.org/

(1) Movement-stabilized three-dimensional optical recordings of membrane potential changes and calcium dynamics in hippocampal CA1 dendritesNeuronMarch 3, 2026Kevin C. Gonzalez, Satoshi Terada, Asako Noguchi, George N. Zakka, Cliodhna O’Toole, Giuliana Bilbao, Luke Reynolds, Anna Jász, Borbála Kertész, Zoltán Szadai, et al.order. 2P photostimulation Mice were injected with a cocktail of 3 viruses: (1) pAAV-Ef1a-FLPo (Addgene #55637-AAV1, titer 8.9E12), (2) pAAV-Ef1a-fDIO-tdTomato (Addgene #128434-AAV1, titer 1.4E+13), (3) pAAV-Ef1a DIO-rsChRmine-oScarlet-Kv2.1-WPRE

(2) A Distinct Circuit for Biasing Visual Perceptual Decisions and Modulating Superior Colliculus Activity through the Mouse Posterior StriatumJ NeurosciFebruary 18, 2026Kara K. Cover, Kerry Elliott, Sarah M. Preuss, Richard J. Krauzlisin wild-type mice, either AAV1-hSyn-cre-WPRE-hGHpA (Addgene #105553-AAV1) or AAV1-Ef1a-flp (Addgene #55637-AAV1) was unilaterally injected into the DMS, with the other virus injected into the PS (same volumes and

(3) A Distinct Circuit for Biasing Visual Perceptual Decisions and Modulating Superior Colliculus Activity through the Mouse Posterior StriatumJ NeurosciJanuary 28, 2026Kara K. Cover, Kerry Elliott, Sarah M. Preuss, Richard J. Krauzlisin wild-type mice, either AAV1-hSyn-cre-WPRE-hGHpA (Addgene #105553-AAV1) or AAV1-Ef1a-flp (Addgene #55637-AAV1) was unilaterally injected into the DMS, with the other virus injected into the PS (same volumes and

(4) Cerebellar climbing fibers impact experience-dependent plasticity in the mouse primary somatosensory cortexeLifeDecember 22, 2025Abby Silbaugh, Kevin P Koster, Christian HanselRRID:Addgene_44362,Titer (GC/ml):2.3×1013 Recombinant DNA reagent,AAV1-EF1a-Flpo (viral prep),Addgene,Addgene #: 55637-AAV1; RRID:Addgene_55637,Titer (GC/ml):8.9×1012 Recombinant DNA reagent,hSyn-Coff/Fon hChR2(H134R)-EYFP

(5) Cerebellar climbing fibers impact experience-dependent plasticity in the mouse primary somatosensory cortexElifeDecember 22, 2025Abby Silbaugh, Kevin P Koster, Christian HanselRRID:Addgene_44362,Titer (GC/ml):2.3×1013 Recombinant DNA reagent,AAV1-EF1a-Flpo (viral prep),Addgene,Addgene #: 55637-AAV1; RRID:Addgene_55637,Titer (GC/ml):8.9×1012 Recombinant DNA reagent,hSyn-Coff/Fon hChR2(H134R)-EYFP

(6) A striosomal accumbens pathway drives stereotyped behavior through an aversive Esr1+ hypothalamic-habenula circuitScience AdvancesNovember 21, 2025Thomas Contesse, Buse Yel Bektash, Marta Graziano, Chiara Forastieri, Alessandro Contestabile, Salome Hahne, Felix Jung, Ifigeneia Nikolakopoulou, Eleonora Rubino, Xiao Cao, et al.(51509-AAV1), AAV1-hSyn-Cre (105553-AAV1), AAVrg-Ef1α-fDIO-mCherry (114471-AAVrg), AAV1-EF1α-Flpo (55637-AAV1), pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (55645-AAV8), pAAV-EF1α-DIO-hChR2(H134R)-EYFP (20298-AAV5), pAAV-EF1α-DIO-hChR2(H134R)-mCherry

(7) Prolonged exposure to food odors suppresses feeding via an olfactory bulb-to-hypothalamus circuitNat CommunAugust 23, 2025Yao Liu, Hailan Liu, Huey-Xian Kelly Wong, Niccole Auld, Kristine M. Conde, Yongxiang Li, Meng Yu, Yue Deng, Qingzhuo Liu, Xing Fang, et al.surgery, we bilaterally injected an anterograde trans-synaptic AAV1 vector carrying Flpo (Addgene, #55637-AAV1) into the vSub, and bilaterally inject Flpo-dependent AAV harboring hM4Di-mCherry (plasmid was kindly

(8) Thalamic integration of basal ganglia and cerebellar circuits during motor learningbioRxivNovember 2, 2024Richard H. Roth, Michael A. Muniak, Charles J. Huang, Fuu-Jiun Hwang, Yue Sun, Cierra Min, Tianyi Mao, Jun B. DingAddgene_26973" AAV1-pENN.AAV.hSyn.Cre.WPRE.hGH,Addgene,"Addgene viral prep # 105553-AAV1, RRID: Addgene_105553" AAV1-EF1a-Flpo,Addgene,"Addgene viral prep # 55637-AAV1, RRID: Addgene_55637" AAV8-hSyn Con/Fon EYFP (AAV8-hSyn-cDIO-fDIO-EYFP),Addgene,"Addgene viral prep

(9) Multistep allelic conversion in mouse pre-implantation embryos by AAV vectorsScientific ReportsAugust 29, 2024Petr Nickl, Irena Jenickova, Jan Elias, Petr Kasparek, Cyril Barinka, Jana Kopkanova, Radislav Sedlacek#62939). pAAV-EF1a-Flpo was a gift from Karl Deisseroth (Addgene plasmid #55637, Addgene viral prep #55637-AAV1). pCAG-NLS-HA-Dre (Addgene plasmid #51272) was a gift from Pawel Pelczar. pCAG-Flpo (Addgene plasmid

(10) A distinct circuit for biasing visual perceptual decisions and modulating superior colliculus activity through the mouse posterior striatumbioRxivAugust 6, 2024Kara K. Cover, Kerry Elliott, Sarah M. Preuss, Richard J. Krauzlisin wild-type mice, either AAV1-hSyn-cre-WPRE-hGHpA (Addgene #105553-AAV1) or AAV1-Ef1a-flp (Addgene #55637-AAV1) was unilaterally injected in the DMS and the other virus in the PS (same volumes and coordinates as

(11) Topographical and cell type-specific connectivity of rostral and caudal forelimb corticospinal neuron populationsCell ReportsApril 23, 2024Lina Marcela Carmona, Eric D. Thomas, Kimberly Smith, Bosiljka Tasic, Rui M. Costa, Anders Nelsonstrains AAV-retro-CAG-tdTomato,Addgene,Cat# 59462-AAVrg AAV1-hSyn-Cre-WPRE.hGh,Addgene,Cat# 105553-AAV1 AAV1-EF1a-Flpo,Addgene,Cat# 55637-AAV1 AAV-retro-hSyn-Cre-WPRE.hGh,Addgene,Cat# 105553-AAVrg AAV-retro-EF1a-Flpo,Addgene,Cat# 55637-AAVrg AAV2-hSyn-ChR2-EYFP-WPRE.hGH,UNC

(12) Amygdala-hippocampus somatostatin interneuron beta-synchrony underlies a cross-species biomarker of emotional stateNeuronApril 3, 2024Adam D. Jackson, Joshua L. Cohen, Aarron J. Phensy, Edward F. Chang, Heather E. Dawes, Vikaas S. Sohal#137140-AAV8 AAV2-Syn-tdTomato,SignaGen Laboratories,SL100896 AAV1-EF1a-Flpo,DOI: 10.1038/nmeth.2996,Addgene; #55637-AAV1 "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(13) The functional and anatomical characterization of three spinal output pathways of the anterolateral tractCell reportsMarch 26, 2024Chen H, Bleimeister IH, Nguyen EK, Li J, Cui AY, Stratton HJ, Smith KM, Baccei ML, Ross SE.Deisseroth,"Addgene,114469-AAV5" pENN.AAV1-hSyn-Cre-WPRE.hGH,James M. Wilson,"Addgene,105553-AAV1" AAV1-EF1a-Flpo,Karl Deisseroth,"Addgene,55637-AAV1" AAV2-hSyn-EGFP,Bryan Roth,"Addgene,50465-AAV2" AAV1.hSyn.HI.eGFP-Cre.WPRE.SV40,James M. Wilson,"Addgene,105540-AAV1" AAV2.hSyn.hChR2(H134R).eYFP.WPREpA,UNC

(14) Monosynaptic trans-collicular pathways link mouse whisker circuits to integrate somatosensory and motor cortical signalsPLOS BiologyMay 19, 2023Jesús Martín-Cortecero, Emilio Ulises Isaías-Camacho, Berin Boztepe, Katharina Ziegler, Rebecca Audrey Mease, Alexander GrohCon/Fon EYFP,Addgene,#55650-AAV8 AAV2-Ef1a-fDIO mCherry,Addgene,#114471-AAV2 AAV1-EF1a-Flpo,Addgene,#55637-AAV1 AAVrg-CAG-hChR2-tdTomato,Addgene,#28017- retrograde AAV1/2-CAG-SyPhy-EGFP,Thomas Kuner Lab,N/A AAV1/2-CBA-SyPhy-mOrange,Thomas

(15) Cerebellar granule cell signaling is indispensable for normal motor performanceCell ReportsMay 3, 2023Joon-Hyuk Lee, Mehak M. Khan, Amanda P. Stark, Soobin Seo, Aliya Norton, Zhiyi Yao, Christopher H. Chen, Wade G. RegehrGABRA6(cre+)-AAV-TOXIN mice and GABRA6(cre-)-AAV-TOXIN mice were injected by pAAV-EF1a-Flpo (Addgene, 55637-AAV1, titer: 5.0 × 10E12 GC/mL) virus in 6 holes with 3 injections (500 nL/injection) a hole with 0.7, 1.7,

(16) Social trauma engages lateral septum circuitry to occlude social rewardNatureJanuary 1, 2023Li L, Durand-de Cuttoli R, Aubry AV, Burnett CJ, Cathomas F, Parise LF, Chan KL, Morel C, Yuan C, Shimo Y, et al.regions (terminal stimulation). For CreOff virus injection, AAV-EF1a-Flpo (2.0 × 1012 vg mL–1, no. 55637-AAV1, Addgene) and AAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (2.0 × 1012 vg mL–1, no. 137141-AAV8, Addgene) were

(17) Activation of VIP interneurons in the prefrontal cortex ameliorates neuropathic pain aversivenessCell reportsSeptember 13, 2022Li M, Zhou H, Teng S, Yang G.#44361 -AAV9 AAV9-hSyn-DIO-mCherry,Addgene,Cat#50459-AAV9 AAV8-CaMKIIα-hM3Dq-mCherry,Addgene,Cat#50476-AAV8 AAV1-CaMKIIα-mCherry,Addgene,Cat#114469-AAV1 AAVrg-Pgk-Cre,Addgene,Cat#24593-AAVrg AAV1-EF1a-FLPo,Addgene,Cat#55637-AAV1 AAV1-EF1a-fDIO-mCherry,Addgene,Cat#114471-AAV1 AAV5-CaMKIIα-EGFP,Addgene,Cat#50469-AAV5 AAV1-CAG-FLEX-EGFP,Addgene,Cat#51502-AAV1 ,, Chemicals,Chemicals,Chemicals ,, Clozapine N-oxide

(18) Prefrontal feature representations drive memory recallNatureAugust 1, 2022Yadav N, Noble C, Niemeyer JE, Terceros A, Victor J, Liston C, Rajasethupathy P.No. 105553-AAV1; titer: ~1.5–2*1013 vg/mL) was injected in LEC, and 300nL of AAV1-Ef1a-flp (Cat No. 55637-AAV1; titer: ~1*1013 vg/mL) in AC, and 700nL of 1:1 mixture of AAV1-CAG-Flex-eGFP (Addgene, Cat No. 515020-AAV1;

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pAAV-EF1a-Flpo (AAV1) from Addgene Inc.

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Addgene Inc.

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pAAV-EF1a-Flpo (AAV1) from Addgene Inc.

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