pAAV-GFAP104-mCherry (AAV5) from Addgene Inc.

Product Specs
ItempAAV-GFAP104-mCherry (AAV5)
CompanyAddgene Inc.
Price Supplier Page View Company Product Page
Catalog Number58909-AAV5
Quantity100 µL
Puritysuitable purity for injection into animals
Speciesrecombinant adeno-associated virus (rAAV)
Molecule NamemCherry
Source/Expression SystemMammalian
Vector NamepAAV-GFAP104-mCherry

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Addgene Inc.

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Watertown, Massachusetts 02472

United States

Phone: +1 (617) 233-4363

Email: help@addgene.org

Company Profile

Website: www.addgene.org/

(1) Cortical astrocyte activation triggers meningeal nociception and migraine-like painPainDecember 1, 2025Dara Bree, Jun Zhao, Jennifer Stratton, Dan LevyBryan Roth (Addgene #50478-AAV5). In control experiments, AAV-GFAP-mCherry was microinjected (Addgene# 58909-AAV5). At the end of the injections, the skin incision was sutured, and animals were given SR meloxicam (4

(2) Ventral striatal astrocytes contribute to reinforcement learningbioRxivOctober 23, 2025Julia Pai, Fatih Sogukpinar, Kei Ogasawara, Garrett J Smith, Francesca R Fiocchi, Yanchao Dai, Yifan Wu, Michael J Frank, ShiNung Ching, Federica Lucantonio, et al.poke. Virus injections. Mice received intercranial injections of control (AAV5-GFAP104-mCherry, Addgene 58909-AAV5, or AAV5-gfaABC1D-tdTomato, Addgene 44332-AAV5), PMCA (AAV5-pZac2.1-GfaABC1D-mCherry-hPMCA2w/b, Addgene

(3) Dorsomedial striatal neuroinflammation causes excessive goal-directed action control by disrupting astrocyte functionNeuropsychopharmacologySeptember 27, 2025Arvie Rodriguez Abiero, Joanne M. Gladding, Jacqueline A. Iredale, Hannah R. Drury, Elizabeth E. Manning, Christopher V. Dayas, Amolika Dhungana, Kiruthika Ganesan, Karly Turner, Serena Becchi, et al.(Addgene, item ID 50479-AAV5, titre 7 × 10¹² vg/mL) or the control AAV-GFAP104-mCherry (Addgene, item ID 58909-AAV5, titre 1 × 10¹³ vg/mL) at the coordinates for pDMS. Behavioural procedures Behavioural procedures

(4) Astrocytopathy Is Associated with CA1 Synaptic Dysfunction in a Mouse Model of Down SyndromeCellsAugust 28, 2025Álvaro Fernández-Blanco, Candela González-Arias, Cesar Sierra, Alfonsa Zamora-Moratalla, Gertrudis Perea, Mara DierssenRRID:Addgene_50478). As a control, we used AAV5-GFAP104-mCherry (gift from Edward Boyden; Addgene viral prep #58909-AAV5). We also used a construct to express a genetically encoded calcium indicator (cyto-GCaMP6f) under the

(5) Striatal astrocytes modulate behavioral flexibility and whole-body metabolism in miceNat CommunJuly 7, 2025Enrica Montalban, Anthony Ansoult, Daniela Herrera Moro Chao, Cuong Pham, Clara Franco, Andrea Contini, Julien Castel, Rim Hassouna, Marene H. Hardonk, Anna Petitbon, et al.Addgene_111067). pAAV-GFAP104-mCherry (6,4 × 1012 vg/ml) was a gift from Edward Boyden (Addgene viral prep # 58909-AAV5; http://n2t.net/addgene:58909; RRID:Addgene_58909). pAAV-GFAP-hM3D(Gq)-mCherry (1.2 × 1013 gc/ml) was

(6) Adenosine 2A Receptors Link Astrocytic Alpha-1 Adrenergic Signaling to Wake-Promoting Dopamine NeuronsBiological PsychiatryMay 1, 2025Nicholas Petersen, Katharine E. McCann, Mihaela A. Stavarache, Lisa Y. Kim, David Weinshenker, Danny G. Winderhttp://n2t.net/addgene:162377; RRID:Addgene_162377, Bacterial or Viral Strain,AAV5-GFAP104-mCherry,Addgene,#58909-AAV5; http://n2t.net/addgene:58909; RRID:Addgene_58909, Bacterial or Viral Strain,AAV5-GFAP-hM3D(Gq)-mCherry,Addgene,#50478-AAV5;

(7) Cortical astrocyte activation triggers meningeal nociception and migraine-like painbioRxivFebruary 18, 2025Dara Bree, Jun Zhao, Jennifer Stratton, Dan LevyBryan Roth (Addgene #50478-AAV5). In control experiments, AAV-GFAP-mCherry was microinjected (Addgene# 58909-AAV5). At the end of the injections, the skin incision was sutured, and animals were given SR meloxicam (4

(8) Chemogenetic Control of Striatal Astrocytes Improves Parkinsonian Motor Deficits in MiceGliaFebruary 4, 2025Wesley R. Evans, Sindhuja S. Baskar, Angelica Vellore, Ana Raquel Castro E. Costa, Cynthia Jacob, Sanya Ravoori, Abimbola Arigbe, Rafiq Hudaof either AAV5‐GFAP‐hM4D(Gi)‐mCherry (Addgene catalog # 50479‐AAV5) or AAV5‐GFAP‐mCherry (Addgene # 58909‐AAV5) was injected into the left dorsal striatum (coordinates: AP: +1.0 mm, ML: +1.7 mm, DV: −2.6 mm, relative

(9) Astrocytic Ca2+ activation by chemogenetics mitigates the effect of kainic acid‐induced excitotoxicity on the hippocampusGliaDecember 1, 2024Nira Hernández‐Martín, María Gómez Martínez, Pablo Bascuñana, Rubén Fernández de la Rosa, Luis García‐García, Francisca Gómez, Maite Solas, Eduardo D. Martín, Miguel A. Pozo1013 GC/mL Addgene 50478‐AAV5) or control virus (pAAV‐GFAP104‐mCherry Titer: 1.2 × 1013 GC/mL Addgene 58909‐AAV5) diluted in artificial cerebrospinal fluid were injected using a 2 μL, 34‐gauge needle Hamilton syringe

(10) Norepinephrine Signals Through Astrocytes To Modulate SynapsesbioRxivSeptember 20, 2024Katheryn B Lefton, Yifan Wu, Allen Yen, Takao Okuda, Yufen Zhang, Yanchao Dai, Sarah Walsh, Rachel Manno, Joseph D Dougherty, Vijay K Samineni, et al.Adeno-associated viruses: AAV5-gfaABC1D::lckGCaMP6f (Addgene #52924-AAV5) was co-injected at a 3:1 ratio with AAV5-gfaABC1D::mCherry (Addgene #58909-AAV5, 1μL with a total of 1×1010 genome copies injected), or at a 1:1 ratio with AAV5-gfaABC1D::hPMCA2-mCherry

(11) Functional activation of dorsal striatum astrocytes improves movement deficits in hemi-parkinsonian micebioRxivApril 6, 2024Wesley R. Evans, Sindhuja S. Baskar, Ana Raquel Castro E Ana Raquel, Sanya Ravoori, Abimbola Arigbe, Rafiq Hudaof either AAV5-GFAP-hM4D(Gi)-mCherry (Addgene catalog # 50479-AAV5) or AAV5-GFAP-mCherry (Addgene # 58909-AAV5) was injected into the left dorsal striatum (coordinates: AP: +1.0mm, ML: +1.7mm, DV: −2.6mm, relative

(12) Cold-induced suspension and resetting of Ca2+ and transcriptional rhythms in the suprachiasmatic nucleus neuronsiScienceDecember 15, 2023Ryosuke Enoki, Naohiro Kon, Kimiko Shimizu, Kenta Kobayashi, Sota Hiro, Ching-Pu Chang, Tatsuto Nakane, Hirokazu Ishii, Joe Sakamoto, Yoshifumi Yamaguchi, et al.strains ,, AAV1-Syn1-nes-jRGECO1a,Addgene,100854-AAV1 AAV1-syn-FLEX-jGCaMP8s-WPRE,Addgene,162377-AAV1 AAV1.hSyn.Cre,Addgene,105553-AAV1 AAV1-hSyn1.Twitch2B,Addgene,100040-AAV1 AAV5-GFAP104-mCherry,Addgene,58909-AAV5 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(13) Serotonergic neurons control cortical neuronal intracellular energy dynamics by modulating astrocyte-neuron lactate shuttleiScienceJanuary 20, 2023Natsubori A, Hirai S, Kwon S, Ono D, Deng F, Wan J, Miyazawa M, Kojima T, Okado H, Karashima A, et al.(Addgene viral prep # 50465-AAV9.T, gifted from Bryan Roth), AAV-GFAP-mCherry (Addgene viral prep # 58909-AAV5, gifted from Edward Boyden),71 and AAV8-hSyn-DIO-hM4D(Gi)-mCherry (Addgene viral prep # 44362-AAV8,

(14) Hypothalamic astrocytes control systemic glucose metabolism and energy balanceCell metabolismOctober 4, 2022Herrera Moro Chao D, Kirchner MK, Pham C, Foppen E, Denis RGP, Castel J, Morel C, Montalban E, Hassouna R, Bui LC, et al.Roth.,Addgene viral prep #50479-AAV5 AAV 2/5.GFAP104.mCherry,gift from Edward Boyden.,Addgene viral prep #58909-AAV5 AAV 2/5.GFAP.Cre.WPRE,gift from James M. Wilson.,Addgene viral prep #105550-AAV5 pAAV.CAG.Flex.GCaMP6f.WPRE,was

(15) A role for glial fibrillary acidic protein (GFAP)-expressing cells in the regulation of gonadotropin-releasing hormone (GnRH) but not arcuate kisspeptin neuron output in male miceeLifeJuly 22, 2021Vanacker C, Defazio RA, Sykes CM, Moenter SM.50478-AAV5,Addgene_50478 AAV,AAV5-GFAP-hM4Di(Gi)-mCherry,AAV-Gi,This paper,Addgene; 50479-AAV5,Addgene_50479 AAV,AAV5-GFAP104-mCherry,AAV-mCherry,This paper,Addgene; 58909-AAV5,Addgene_58909 Mouse strains and viruses used.

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pAAV-GFAP104-mCherry (AAV5) from Addgene Inc.

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Addgene Inc.

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pAAV-GFAP104-mCherry (AAV5) from Addgene Inc.

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