Fig 1: (A) List of proteins found with at least one combination of an SRSF1 fusion to AP or EX and a helicase, TNPO3 or SRRM2 fused to EX or AP. Each combination was tested in three separate experiments, and each dataset lists proteins found in at least two experiments. These proteins are arranged in blocks according to the number of datasets in which the proteins were found, indicated by the depth of colour of the block. (B) The proteins in the DDX46-SRSF1 dataset are listed in blocks according to the number of other helicase datasets in which they were found. Within each block the proteins are ranked according to the total 679 spectral counts of each protein (highest at the top; dark purple). The same proteins in the other datasets are coloured according to their ranking in the total spectral counts in that dataset.
Fig 2: Effects of split-APEX combinations on the efficiency of biotinylation. AP or EX fragments of APEX2 fused to SRSF1 were co-transfected into HEK293T cells with EX or AP fragments fused to the helicases, TNPO3 or SRRM2. Cells were lysed in 8 M urea and the equivalent of 40 µg of total protein content was analysed by SDS-PAGE. Biotinylation was detected by Western blotting with dye-labelled streptavidin (upper panel) and equal loading and transfer were shown by staining the membrane with Ponceau S (lower panel).
Supplier Page from Sino Biological, Inc. for Human TNPO3 Gene ORF cDNA clone in cloning vector