Fig 1: SMC1A promoted gastric cancer cell proliferation, invasion and migration. A The expression of SMC1A was examined in SMC1A silenced and overexpressed cells by western blot method. CCK-8 assay (B) and Colony formation assay (C) were used to determine the effect of SMC1A knockdown and overexpression on cell proliferation. Matrigel invasion assay (D) and Wound healing assay (E) analyzed the effect of SMC1A knockdown and overexpression on cell invasion and migration respectively. F Subcutaneous xenografts of AGS cells transfected with SMC1A overexpression plasmids. Images of tumors from nude mice at autopsy are presented (left), the tumor volumes were measured at the indicated time points (middle), and the average weight of the xenografted tumors was measured (right). *P < 0.05, **P < 0.01, ***P < 0.001
Fig 2: SMC1A promoted GC epithelial-mesenchymal transition (EMT) via upregulating SNAIL. A SNAIL level was examined in SMC1A silenced and overexpressed cells using western blot method. B Proteins level of EMT markers E-cadherin, N-cadherin and Vimentin were detected after SMC1A depletion and overexpression. Immunofluorescence assay detected the expression of E-cadherin (C) and Vimentin (D) in SMC1A silenced and overexpressed cells. E The expression of SNAIL was examined in SNAIL overexpressed cells by western blot method. F Proteins level of EMT markers E-cadherin, N-cadherin and Vimentin were detected in response to the treatment of SMC1A siRNA and SMC1A siRNA + SNAIL
Fig 3: SMC1A promoted GC cell proliferation, invasion and migration via SNAIL. CCK-8 assay (A) and Colony formation assay (B) were performed to analysis cell proliferation in response to the treatment of SMC1A siRNA and SMC1A siRNA + SNAIL. Matrigel invasion assay (C) and Wound healing assay (D) used to investigate cell invasion and migration in response to the treatment of SMC1A siRNA and SMC1A siRNA + SNAIL. *P < 0.05
Fig 4: SMC1A was overexpressed in human gastric cancer. A The expression of SMC1A was analyzed in cancer tissues from TCGA data. blue: adjacent normal tissues and red: gastric cancer tissues. Western blot (B) and RT-qPCR (C) analysis for SMC1A mRNA in 20 samples of GC tissues and the corresponding adjacent tissues. D The overall survival of GC patients was evaluated using Kaplan-Meier Plotter. The red line represents SMC1A high expression group, and black line represents SMC1A low expression group. The expression of SMC1A was examined in human gastric cancer cell lines and the human gastric epithelial cell line GES-1using RT-qPCR method (E) and western blot method (F). **P < 0.01, **P < 0.001, ***P < 0.001
Supplier Page from Sino Biological, Inc. for Human SMC1A Gene ORF cDNA clone expression plasmid