Description
Designed with 4 genomically integratable barcodes that, not only can be differentiated by sequencing, but also by PCR and qPCR In addition to having a unique sequence, common primer sequences for each barcode amplify a different size product so populations labeled with a specific barcode can be identified on an agarose gel. Further, each barcode has a unique qPCR probe sequence that enables differential amplification, and quantitation using qPCR