pRSITEP-U6Tet-(xx)-EF1-TetRep-2A-Puro (linearized) from Cellecta

Product Specs
ItempRSITEP-U6Tet-(xx)-EF1-TetRep-2A-Puro (linearized)
CompanyCellecta
Price Supplier Page View Company Product Page
Catalog NumberSVSHU6TEP-L
Quantity500 ng (50 rxns)
ApplicationsCloning Vector for shRNA
Sample TypeLentiviral
SpeciesMammalian
TypeVector
Vector NamepRSITEP-U6Tet-(xx)-EF1-TetRep-2A-Puro

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Cellecta

320 Logue Ave

Mountain View, California 94043

United States

Phone: 650-938-3910
Toll free: 877-938-3910
Fax: 650-938-3911

Company Profile

Website: http://www.cellecta.com

(1) Conserved and unique features of terminal telomeric sequences in ALT-positive cancer cellseLifeAugust 1, 2025Benura Azeroglu, Wei Wu, Raphael Pavani, Ranjodh Singh Sandhu, Tadahiko Matsumoto, André Nussenzweig, Eros Lazzerini-DenchiRRID:AB_10003037",IF (1:1000) Recombinant DNA reagent,pRSIT16-U6Tet-sh-EF1-TetRep-2A-Puro,Cellecta,Cat# SVSHU6TEP-L,Plasmid Recombinant DNA reagent,pLenti- CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT,"Dilley et al.,

(2) DBeQ derivative targets vacuolar protein sorting 4 functions in cancer cells and suppresses tumor growth in miceThe Journal of Pharmacology and Experimental TherapeuticsApril 1, 2025Kevin A. Fundora, Yan Zhuang, Kouta Hamamoto, Guifang Wang, Longgui Chen, Tatsuya Hattori, Xinwen Liang, Lei Bao, Venugopal Vangala, Fang Tian, et al.Supplemental Table 1 were subcloned into the BsbI site of pRSITEP-U6Tet-(shRNA)-EF1-TetRep-2A-Puro (Cellecta; SVSHU6TEP-L). pCDH-TRE3G-GFP-EF1α-Puro-tPT2A-Tet-On 3G, pCDH-TRE3G-GFP-VPS4AE228Q-EF1α-Puro-tPT2A-Tet-On 3G, pCDH1-CMV-MCS-SV40-Hygro,

(3) Identification of modulators of the ALT pathway through a native FISH-based optical screenCell reportsJanuary 28, 2025Azeroglu B, Khurana S, Wang SC, Tricola GM, Sharma S, Jubelin C, Cortolezzis Y, Pegoraro G, Miller KM, Stracker TH, et al.vector,Addgene,41824 pcDNA6.2/N-EmGFP-DEST,ThermoFisher,Cat #V35620 pRSIT16-U6Tet-sh-EF1-TetRep-2A-Puro,Cellecta,SVSHU6TEP-L pLentiCas9-Blast,Addgene,52962 pLenti- CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT,"Dilley et al.,101",N/A pLenti-

(4) Identifying regulators of aberrant stem cell and differentiation activity in colorectal cancer using a dual endogenous reporter systemNat CommunMarch 12, 2024Sandor Spisak, David Chen, Pornlada Likasitwatanakul, Paul Doan, Zhixin Li, Pratyusha Bala, Laura Vizkeleti, Viktoria Tisza, Pushpamali De Silva, Marios Giannakis, et al.before proceeding to plasmid propagation. pRSITEP-U6Tet-sh-noHTS-EF1-TetPep-2A-Puro plasmid (Cellecta #SVSHU6TEP-L-CT) was used for shRNA inducible knockdown in colon organoids. The plasmid was digested with BbsI (NEB

(5) A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in CarcinomasCancer discoveryMarch 1, 2023Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer SM, Shim BH, Dumont N, et al.the cloning adapters were inserted into pRSITEP-U6Tet-sh-EF1-TetRep-2A-Puro (Cellecta, catalog no. SVSHU6TEP-L) or pRSITEP-U6Tet-sh-EF1-TetRep-2A-Hygro, both of which enable doxycycline-inducible shRNA expression.

(6) Phosphate dysregulation via the XPR1â€“KIDINS220 protein complex is a therapeutic vulnerability in ovarian cancerNature CancerJune 1, 2022Daniel P. Bondeson, Brenton R. Paolella, Adhana Asfaw, Michael V. Rothberg, Thomas A. Skipper, Carly Langan, Gabriel Mesa, Alfredo Gonzalez, Lauren E. Surface, Kentaro Ito, et al.accomplished by cloning these sequences into the pRSITEP-U6Tet-(shRNA)-EF1-TetRep-2A-Puro vector (Cellecta #SVSHU6TEP-L). shRNA seed matched negative control sequences62 were generated by substituting complementary base

(7) Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16qCell reportsDecember 15, 2020Neggers JE, Paolella BR, Asfaw A, Rothberg MV, Skipper TA, Yang A, Kalekar RL, Krill-Burger JM, Dharia NV, Kugener G, et al.(McDonald et al., 2017) and cloned into the pRSITEP-U6Tet(shRNA)-EF1-TetRep-2A-Puro vector (Cellecta #SVSHU6TEP-L) for doxycycline inducible shRNA expression. Negative control shRNA seed sequences were generated for

(8) WRN helicase is a synthetic lethal target in microsatellite unstable cancersNatureApril 1, 2019Chan EM, Shibue T, McFarland JM, Gaeta B, Ghandi M, Dumont N, Gonzalez A, McPartlan JS, Li T, Zhang Y, et al.puro (Addgene #8453) for constitutive expression and pRSITEP-U6Tet-(xx)-EF1-TetRep-2A-Puro (Cellecta #SVSHU6TEP-L) for doxycycline-inducible shRNA expression. All in vitro experiments used 0.2 μg/mL of doxycycline

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pRSITEP-U6Tet-(xx)-EF1-TetRep-2A-Puro (linearized) from Cellecta

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Cellecta

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pRSITEP-U6Tet-(xx)-EF1-TetRep-2A-Puro (linearized) from Cellecta

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