Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep) from Addgene Inc.

Product Specs
ItemHuman sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)
CompanyAddgene Inc.
Price Supplier Page View Company Product Page
Catalog Number73178-LV
Quantity13 mL
Sample TypePooled Library, Ready-to-Use Lentiviral Prep
Source/Expression SystemMammalian
Puritysuitable purity for mammalian cell culture
TypesgRNA library
SpeciesLentivirus
Molecule Name76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls
FormatLentiviral

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Addgene Inc.

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Watertown, Massachusetts 02472

United States

Phone: +1 (617) 233-4363

Email: help@addgene.org

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Website: www.addgene.org/

(1) Identification of CD164 as an essential entry receptor for divergent adeno-associated virusesProc Natl Acad Sci U S AMarch 10, 2026Xiujuan Zhang, Donovan Richart, Shane McFarlin, Fang Cheng, Soo Yeun Park, Anwen Zhang-Chen, Richenda McFarlane, Chuan Xiao, Ziying Yan, Jianming QiuGenome-Wide CRISPR/Cas9 Screen, NGS, and Bioinformatics Analysis. The Brunello lentiCRISPR gRNA library (#73178-LV, Addgene) was used for the genome-wide CRISPR/Cas9 screen. A detailed method can be found in SI Appendix. rAAV

(2) High-throughput Genome Wide CRISPR Knock Out mechanical sort identifies genes driving metastatic cancer cell softeningbioRxivFebruary 17, 2026Katherine M Young, Curtis Dobrowolski, Nicholas Stone, Kalina Paunovska, Sydney Bules, Kelly Ahkee, James Hankish, Alex Chapman, James E Dahlman, Todd A Sulchek, et al.lentiviral pooled library was designed to optimize on-target activity and reduce off-target effects (Addgene 73178-LV)25. This library targets 19,114 human genes with 76,441 total gRNAs, with 3–4 gRNAs targeting each gene.

(3) SETDB1/ATF7IP regulate the precise genome engineering of HUSH-regulated genesEpigenetics ChromatinFebruary 9, 2026Brian L. Ruis, Henry Ward, Chad L. Myers, Anja-Katrin Bielinsky, Eric A. Hendrickson2.4 × 108 HAP1 ssDI reporter cells were transduced with the Brunello lentiGuide-Puro library (Addgene 73178-LV) at 2.88 × 108 CFU/ml at an MOI of 0.3 and 500-fold coverage (day 1) and allowed to recover for one

(4) A whole genomic CRISPR-Cas9 screen identifies the amino acid transporter SLC43A1 (LAT3) as a major determinant of oxaliplatin sensitivity in colorectal cancer cellsbioRxivJanuary 16, 2026N R Pawar, H M Wade, Z Jackson, N Poungpeth, A V Mitchell, C P Jewell, D Chan, R W Robey, P J Batista, L M Jenkins, et al.Brunello CRISPR knockout pooled library was a gift from David Root and John Doench (Addgene viral prep #73178-LV) (18). The Human Calabrese CRISPR Activation Pooled Library Set A was a gift from David Root and John

(5) SIGLEC12 mediates plasma membrane rupture during necroptotic cell deathNatureJanuary 8, 2026Hyunjin Noh, Zeena Hashem, Elena Boms, Ayaz Najafovtilt. Genome-wide CRISPR-based knockout screens Human Brunello sgRNA library (Addgene, catalogue no. 73178-LV) was used40. This library contains 76,441 sgRNAs targeting 19,114 genes (4 sgRNAs per gene), with 1,000

(6) DNA Damage Sensing and TP53 Function as Modulators of Sensitivity to Calicheamicin-Based Antibody–Drug Conjugates for Acute LeukemiaCancers (Basel)December 25, 2025Camryn M. Pettenger-Willey, George S. Laszlo, Margery Gang, Frances M. Cole, Colin D. Godwin, Sarah Erraiss, Pritha Chanana, Allie R. Kehret, Junyang Li, Jacob W. Barton, et al.including 76,441 unique gRNAs covering over 19,000 genes, each gene targeted with 4 unique sgRNAs (ID # 73178-LV), were all purchased from Addgene (Watertown, MA, USA). The CD33 knockout (KO) sgRNA targeting exon

(7) A CRISPR-based genome-wide loss-of-function screen defines a role of host metabolism in regulating hepatitis B virus infectionMolecular TherapyDecember 1, 2025Tadashi Inuzuka, Karim Mouzannar, Min Zhang, Regina Umarova, Seung Bum Park, Takuro Uchida, Christopher D. Ma, T. Jake Lianginfection. Pooled sgRNA library A pooled human sgRNA library, ‘Brunello’, from Broad Institute (Lentiviral Prep #73178-LV, Addgene, MA, USA) was used. This library targets 19,114 genes with a total of 76,441 sgRNAs. To achieve

(8) CRISPR/Cas9 Screenings Reveal the Role of STX1A and CDK1 in Cathepsin G Entering and Killing Colorectal Cancer CellsInterdiscip Inf SciNovember 15, 2025Yuxiang WANG, Valery ROZEN, Trang DINH, He LI, Yamu LI, Yiqing ZHAO, Zhenghe WANGperformed as described previously [20]. Briefly, the Human CRISPR Knockout Pooled Library (Brunello, #73178-LV, Addgene) was employed to induce genome-wide knockout in DLD1 cells stably expressing Cas9. DLD1-Cas9

(9) CRISPR/Cas9 Screenings Reveal the Role of STX1A and CDK1 in Cathepsin G Entering and Killing Colorectal Cancer CellsInterdisciplinary Information SciencesNovember 15, 2025Yuxiang WANG, Valery ROZEN, Trang DINH, He LI, Yamu LI, Yiqing ZHAO, Zhenghe WANGperformed as described previously [20]. Briefly, the Human CRISPR Knockout Pooled Library (Brunello, #73178-LV, Addgene) was employed to induce genome-wide knockout in DLD1 cells stably expressing Cas9. DLD1-Cas9

(10) Quiescent OXPHOS-High Triple-Negative Breast Cancer Cells That Persist After Chemotherapy Depend on BCL-XL for SurvivalCellsOctober 8, 2025Slawomir Andrzejewski, Marie Winter, Leandro Encarnacao Garcia, Olusiji Akinrinmade, Francisco Madeira Marques, Emmanouil Zacharioudakis, Anna Skwarska, Julio Aguirre-Ghiso, Marina Konopleva, Guangrong Zheng, et al.expressed the Cas9 DNA endonuclease were transfected with the Brunello lentiviral LentiCRISPR v2 library (#73178-LV, Addgene, Watertown, MA, USA) at an MOI of 70% in medium containing polybrene (8 µg/mL) for 12 h, similarly

(11) Quiescent OXPHOS-high triple-negative breast cancer cells that persist after chemotherapy depend on BCL-XL for survivalbioRxivAugust 29, 2025Slawomir Andrzejewski, Marie Winter, Leandro Encarnacao Garcia, Olusiji Akinrinmade, Francisco D.M. Marques, Emmanouil Zacharioudakis, Anna Skwarska, Julio Aguirre-Ghiso, Marina Konopleva, Guangrong Zheng, et al.Cas9 DNA endonuclease were transfected with the Brunello lentiviral LentiCRISPR v2 library (Addgene #73178-LV) at a MOI of 70% in medium containing polybrene (8μg/mL) for 12h, similarly to our previous studies

(12) Genome-Wide CRISPR Screening Reveals that mTOR Inhibition Initiates Ferritinophagy and Ferroptosis in Head and Neck CancerCancer ResAugust 15, 2025Keiichi Koshizuka, Xingyu Wu, Kuniaki Sato, Pham Thuy Tien Vo, Gosia M. Murawska, Tomohiko Ishikawa, Zhiyong Wang, Alfredo A. Molinolo, Edward A. Dennis, Cherie-Ann O. Nathan, et al.Human genome-wide CRISPR pooled library (Brunello) that was a gift from John Doench & David Root (#73178-LV, Addgene, RRID:Addgene_73178). And cells were amplified and seeded into 500cm2 square cell culture plate

(13) Genome-Wide CRISPR Screening Reveals That mTOR Inhibition Initiates Ferritinophagy and Ferroptosis in Head and Neck CancerCancer ResearchAugust 15, 2025Keiichi Koshizuka, Xingyu Wu, Kuniaki Sato, Pham Thuy Tien Vo, Gosia M. Murawska, Tomohiko Ishikawa, Zhiyong Wang, Alfredo A. Molinolo, Edward A. Dennis, Cherie-Ann O. Nathan, et al.Human genome-wide CRISPR pooled library (Brunello) that was a gift from John Doench & David Root (#73178-LV, Addgene, RRID:Addgene_73178). And cells were amplified and seeded into 500cm2 square cell culture plate

(14) D-cysteine impairs tumour growth by inhibiting cysteine desulfurase NFS1Nat MetabAugust 12, 2025Joséphine Zangari, Oliver Stehling, Sven A. Freibert, Kaushik Bhattacharya, Florian Rouaud, Veronique Serre-Beinier, Kinsey Maundrell, Sylvie Montessuit, Sabrina Myriam Ferre, Evangelia Vartholomaiou, et al.screen Cas9-expressing A549 cells were infected with the human CRISPR Brunello lentiviral pooled library (Addgene, 73178-LV) at a multiplicity of infection of 0.3 such that every gRNA is represented in 500 cells. After 48 h,

(15) Resistance Signatures Manifested in Early Drug Response in Cancer and Across SpeciesbioRxivMay 18, 2025Cole Ruoff, Allision Mitchell, Priya Mondal, Vishaka Gopalan, Arashdeep Singh, Michael Gottesman, Sridhar HannenhalliDNA,Recombinant DNA Cas9,Addgene,# 52962-LV Human CRISPR Knockout Pooled Library (Brunello),Addgene,# 73178-LV ,, ,, Software and algorithms,Software and algorithms,Software and algorithms Seurat version 5.2.1,CRAN

(16) Genome-wide CRISPR screen identifies IRF1 and TFAP4 as transcriptional regulators of Galectin-9 in T cell acute lymphoblastic leukemiaSci AdvMarch 21, 2025Caroline R. M. Wiggers, Burak Yüzügüldü, Nathanial G. Tadros, Tayla B. Heavican-Foral, Eugene Y. Cho, Zachary C. Eisenbies, Merve Ozdemir, Steffen B. Kulp, Yun-Cheol Chae, Alejandro Gutierrez, et al.Cas9 activity assay, were infected with the genome-wide Brunello library lentiviral prep (Addgene no. 73178-LV) to achieve 651-fold coverage per sgRNA at an MOI of 0.25. Cells were selected with puromycin (1 μg/ml)

(17) Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectorsmBioJanuary 8, 2025Zhang X, Hao S, Feng Z, Ning K, Aksu Kuz C, McFarlin S, Richart D, Cheng F, Zhang-Chen A, McFarlane R, et al.Cas9-expressing cells (blasticidin-resistant) were then transduced with the Brunello lentiCRISPR gRNA library (#73178-LV, Addgene) and selected with puromycin. The double-resistant cells were expanded for rAAV5 transduction,

(18) A whole-genome CRISPR screen identifies the spindle accessory checkpoint as a locus of nab-paclitaxel resistance in a pancreatic cancer cell lineScientific ReportsJuly 10, 2024Priya Mondal, George Alyateem, Allison V. Mitchell, Michael M. Gottesmanrecruiting repressive chromatin modifiers. The human CRISPR Brunello lentiviral pooled KO library (Addgene # 73178-LV)14 and human CRISPR Dolcetto (Set A) inhibition library (Addgene # 92386-LV)15 were used to identify

(19) Genome-scale CRISPR Cas9a knockout screen reveals genes that control glioblastoma susceptibility to the alkylating agent temozolomideAll LifeDecember 31, 2022Chidiebere U. Awah, Jan Winter, Olorunseun O. Ogunwobiconfluency before downstream analysis. CRISPR Knockout library: The Brunello library pooled gRNA (Cat no: 73178-LV) were commercially purchased from Addgene. This library contains 70,000sgRNA, with 60,000 guides targeting

(20) VCP suppresses proteopathic seeding in neuronsMolecular NeurodegenerationDecember 1, 2022Jiang Zhu, Sara Pittman, Dhruva Dhavale, Rachel French, Jessica N. Patterson, Mohamed Salman Kaleelurrrahuman, Yuanzi Sun, Jaime Vaquer-Alicea, Gianna Maggiore, Christoph S. Clemen, et al.Cas9-blast cells were plated and then infected with pooled lentivirus with Brunello gRNA library (Addgene #73178-LV) with 8μg/ml polybrene (MOI = 0.3) the next day. After 24 h, cells would undergo 1μg/ml puromycin selection.

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Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep) from Addgene Inc.

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Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep) from Addgene Inc.

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