Description
This surface consists of a mixed, self-assembled monolayer of alkanethiolates generated from the combination of polyethylene glycol-terminated alkanethiol (90%) and COOH-terminated alkanethiol (10%). The terminal polyethylene glycol chains minimize non-specific binding while the COOH groups provide a functional attachment site for immobilizing/capturing a molecule of interest. Amine coupling, utilizing EDC/NHS chemistry, is the most common ligand immobilization approach but thiol, aldehyde, and maleimide coupling are also possible with this surface using the appropriate cross-linking chemistry.