BspEI from New England Biolabs

Product Specs
ItemBspEI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0540S
Concentration10,000 units/ml
Quantity1000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

T/CCGGA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based constructMolecular Therapy OncologyMarch 19, 2026Alexandros Kourtesakis, Hiu Nam Hannah Chow, Eileen Bailey, Sandra Horschitz, Ammar Jabali, Rainer Will, Christoph Schifflers, Abigail K. Suwala, Hannah Rohdjess, Melissa Hahn, et al.cloned into the CD27z vector after double digestion with SphI (#R3182S, New England Biolabs) and BspEI (#R0540S, New England Biolabs), using T4 DNA Ligase (# New England Biolabs, M0202S). For cloning into the LF28z/LFBBz

(2) An A-rich linker between dengue virus tandem xrRNAs facilitates functional coordinationbioRxivMarch 4, 2026Elizabeth Spear, Zoe O'Donoghue, Steve L. Bonilla, Kevin P. Larsen, Madeline E Sherlock, Jeffrey S. Kieftsequential digest of 0.2–0.5 μg plasmid DNA with both restriction enzymes (AgeI-HF- NEB R3352L, BspEI- NEB R0540L), as well as Sanger sequencing of the mutagenized region with a primer complementary to the NS5 region

(3) Fluorescent indicators for visualizing dynamic contact between cells and between processes originating from a single cellCell Reports MethodsFebruary 23, 2026Takashi Kanadome, Natsumi Hoshino, Susumu Jitsuki, Hidehiko Hashimoto, Takeshi Yagi, Takeharu Nagairecombinant proteins" ,, NheI,Takara,Cat# 1241A KpnI,Takara,Cat# 1068AH BspEI,New England Biolabs,Cat# R0540S BglII,Takara,Cat# 1021A MluI,Takara,Cat# 1071A EcoRI,Takara,Cat# 1040A NotI,Takara,Cat# 1166A SphI,Takara,Cat#

(4) Fluorescent protein tags for human tropomyosin isoform comparisonBiology OpenAugust 15, 2025Will Scott, Vitaliia Polutranko, Jakub Milczarek, Ian Hands-Portman, Mohan K. Balasubramanian(AddGene, #127912) vector was linearised via restriction digestion with BspEI (New England Biolabs, #R0540). Human-codon-optimised G-blocks (IDT) were synthesised for each of the chosen tropomyosin isoforms

(5) tRNA lysidinylation is essential for the minimal translation system in the Plasmodium falciparum apicoplastEMBO ReportsMarch 20, 2025Rubayet Elahi, Sean T PriggeInc.,Cat # R3189S NgoMIV,New England Biolabs Inc.,Cat # R0564S BspEI,New England Biolabs Inc.,Cat # R0540S BsiWI,New England Biolabs Inc.,Cat # R3553S BsaI,New England Biolabs Inc.,Cat # R3733S T4 DNA ligase,New England Biolabs

(6) Deciphering the functional impact of Alzheimer’s Disease-associated variants in resting and proinflammatory immune cellsmedRxivSeptember 20, 2024Marielle L. Bond, Ivana Y. Quiroga-Barber, Susan D’Costa, Yijia Wu, Jessica L. Bell, Jessica C. McAfee, Nicole E. Kramer, Sool Lee, Mary Patrucco, Douglas H. Phanstiel, et al.using the aforementioned method. We validated the correct insert by digesting with BspEI (Kpn2I) (NEB, #R0540S) and MluI, and with sanger sequencing of the region. Colonies with the correct insert served as the

(7) Development of a Ready-to-Use-Type RNA Vaccine Carrier Based on an Intracellular Environment-Responsive Lipid-like Material with Immune-Activating Vitamin E ScaffoldsPharmaceuticsNovember 29, 2023Anindita J, Tanaka H, Oyama R, Hagiwara S, Shirane D, Taneichi S, Nakai Y, Tange K, Hatakeyama H, Sakurai Y, et al.ovalbumin (OVA). The pcDNA3.1-Luc or pcDNA3.1-OVA were linearized with the restriction enzymes BspEI (#R0540S, New England Biolabs, Ipswich, MA, USA) or XhoI (#R0146S, New England Biolabs, Ipswich, MA, USA), respectively.

(8) Nonlinear control of transcription through enhancer–promoter interactionsNatureApril 21, 2022Jessica Zuin et al.left homology arm was cloned using Gibson assembly strategy by linearizing the vector with BspEI (NEB, R0540). The right homology arm was cloned using Gibson assembly strategy by linearizing the vector with BclI

(9) SLFN11 Inactivation Induces Proteotoxic Stress and Sensitizes Cancer Cells to Ubiquitin Activating Enzyme Inhibitor TAK-243Cancer ResearchJune 1, 2021Yasuhisa Murai et al.into MCS-BioID2-HA was a gift from Kyle Roux (74224; Addgene plasmid) by NheI (R0131; NEB) and BspEI (R0540; NEB) restriction enzyme sites (32). HEK293 cells were transiently transfected with empty vector and

(10) Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection SystemBio-protocolJanuary 20, 2021Landry LG, Mann SE, Anderson AM, Nakayama M.catalog number: 12260) 33. Restriction enzymes (New England Biolabs) a. BspEI (NEB, catalog number: R0540S) b. HindIII (NEB, catalog number: R0104S) c. MfeI (NEB, catalog number: R0589S) d. HpaI (NEB, catalog

(11) Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion CellsNeuronAugust 21, 2019Bray ER, Yungher BJ, Levay K, Ribeiro M, Dvoryanchikov G, Ayupe AC, Thakor K, Marks V, Randolph M, Danzi MC, et al.(Bio-Rad). Cloning: Enzymes used: AgeI (NEB, R3552), BamHI (NEB, R3136), BglII (NEB, R0144), BlpI (NEB, R0585), BspEI (NEB, R0540) EcoRI (NEB, R3101), HindIII (NEB, R3104), KpnI (NEB, R3142), MfeI (NEB, R3589), MluI (NEB, R3198),

(12) Ectopic BASL Reveals Tissue Cell Polarity throughout Leaf Development in Arabidopsis thalianaCurrent BiologyAugust 20, 2018Catherine Mansfield, Jacob L. Newman, Tjelvar S.G. Olsson, Matthew Hartley, Jordi Chan, Enrico CoenProteins" ,, Propidium Iodide,Sigma-Aldrich,Cat# P4170 Spe I,Sigma-Aldrich,11008943001 BspEI,New England Biolabs,Cat# R0540S ccdB-resistant one-shot E. coli,ThermoFisher Scientific,Cat# A10460 Difco agar,Becton & Dickinson,Cat#

(13) Global Inhibition with Specific Activation: How p53 and MYC Redistribute the Transcriptome in the DNA Double-Strand Break ResponseMolecular cellSeptember 21, 2017Porter JR, Fisher BE, Baranello L, Liu JC, Kambach DM, Nie Z, Koh WS, Luo J, Stommel JM, Levens D, et al.inducible MYC-GFP and GFP expression. The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested

(14) TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signallingNatureMay 18, 2017Wang B, Jie Z, Joo D, Ordureau A, Liu P, Gan W, Guo J, Zhang J, North BJ, Dai X, et al.to amplify the DNA fragment containing the K305/K313 site. The PCR products were cut by BspEI (NEB, R0540L) to screen the potential correct clones. Finally, knock-in mutations were verified by the Sanger sequencing

(15) A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinningNature ProtocolsJune 11, 2009Florence Teulé, Alyssa R Cooper, William A Furin, Daniela Bittencourt, Elibio L Rech, Amanda Brooks, Randolph V Lewisantibodies Restriction endonucleases: BamHI, BspEI, NdeI, ScaI, XmaI (New England Biolabs Inc., cat. no. R0136S, R0540S, R0111S, R0122S, R0180S, respectively) T4 DNA ligase (Promega, cat. no. M1801) Lysozyme (Aldrich-Sigma

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BspEI

BspEI from New England Biolabs

Description

Sequence

New England Biolabs

Citations (15)

Reviews

BspEI from New England Biolabs

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