XmnI from New England Biolabs

Product Specs
ItemXmnI
CompanyNew England Biolabs
Price $70.00 Inquire
Catalog NumberR0194S
Concentration20 u/µL
Quantity1000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GAANN/NNTTC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Insights from pooled CRISPRi single-cell screens in K562 cells reveal gene functions, regulatory networks, and highlight opportunities and limitationsBMC GenomicsMarch 13, 2026Hong Zhang, Peifen Zhang, Eric Bindels, Eskeatnaf Mulugetathe Miniprep kit (QIAGEN, catalog no. 27104). All plasmids were verified by XmnI (NEB, catalog no. R0194S) digest and Sanger sequencing with the primer 5’-CCTCGGCCTCTGCATAAATA-3’. dCas9-KRAB K562 cells were

(2) Rbm8a deficiency causes hematopoietic defects by modulating Wnt/PCP signalingDevelopmental BiologyDecember 1, 2025Agnese Kocere, Elena Chiavacci, Charlotte Soneson, Seth T. Jacobson, Emma N. Harrison, Kevin Manuel Méndez-Acevedo, Jacalyn S. MacGowan, Harrison H. Wells, Max S. Hiltabidle, Azhwar Raghunath, et al.Additionally, the rbm8a wild type allele was genotyped by restriction digest of the PCR product with Xmn1 (R0194S, NEB) and the rbm8aΔ3 allele by Hinf1 (R0155S, NEB) while the rbm8aΔ5 allele stayed intact upon Xmn1

(3) The pH-dependent photophysical and spectral properties of pH-sensing green fluorescent proteinsPhysiol RepOctober 18, 2025Ian M. Thornellas gBlocks with XmnI and NotI restriction sites. The gBlocks were digested with restriction enzymes (R0194S and R3189S New England Biolabs), then ligated into their respective vectors using a T7 DNA Ligase Kit

(4) The mammalian Ku70 C-terminus SAP domain is required to repair DNA damageNucleic Acids ResJune 11, 2025Yuan Wang, Michael S Czap, Hailey Kim, Paul M Masaka, Hongxin Wang, Md Amjad Beg, Huimei Lu, Jingmei Liu, Yoke-Chen Chang, Peter J Romanienko, et al.solution (Syd Labs, MB067-EQ2B-L). Following PCR, the product was digested with the XmnI enzyme (NEB, R0194L) at 37°C for 3 h. The digested PCR product was then loaded for agarose gel electrophoresis. Collection

(5) Novel role of zinc-finger protein 518 in heterochromatin formation on α-satellite DNANucleic Acids ResearchJanuary 11, 2025Shinya Ohta, Jun-Ichirou Ohzeki, Nobuko Sato, Hideki Tanizawa, Claire Yik-Lok Chung, Ken-Ichi Noma, Hiroshi Masumotoentry vector (pENTR™ 4 Dual Selection Vector; #A10465, Thermo Fisher Scientific) between the XmnI (#R0194S, New England BioLabs [NEB]) and EcoRV (#R0195S, NEB) sites using the In-Fusion HD Cloning Kit (#Z9648N,

(6) DNA nicks in both leading and lagging strand templates can trigger break-induced replicationMolecular CellJanuary 2, 2025Yuanlin Xu, Carl A. Morrow, Yassine Laksir, Orla M. Holt, Kezia Taylor, Costas Tsiappourdhi, Patrick Collins, Su Jia, Christos Andreadis, Matthew C. Whitbyand NheI-HF (New England Biolabs, R3131L) (Figures 1 and S1) or 200 units XmnI (New England Biolabs, R0194L) (Figure S4), along with 20 μl of RNase A (100 mg/ml; Qiagen; 1007885), were added. The mixture was

(7) Control of DNA replication in vitro using a reversible replication barrierNature ProtocolsJuly 1, 2024Emma J. Vontalge, Tamar Kavlashvili, Steven N. Dahmen, Matthew T. Cranford, James M. DewarDimethyl sulfoxide (DMSO) (Sigma Aldrich, cat. no. D8418) Glycogen (Roche, cat. no. 10901393001) XmnI (NEB, cat. no. R0194S) AlwNI (NEB, cat. no. R0514S) Trichloroacetic acid (TCA) (Thermo Fisher Scientific, cat. no. FLA322500)

(8) BRCA2 promotes genomic integrity and therapy resistance primarily through its role in homology-directed repairMolecular CellFebruary 1, 2024Pei Xin Lim, Mahdia Zaman, Weiran Feng, Maria JasinTween® 20,Sigma-Aldrich,Cat# P9416 VE821 ATR inhibitor,Sigma-Aldrich,Cat# SML1415–5MG XmnI restriction enzyme,NEB,Cat# R0194S Critical commercial assays,Critical commercial assays,Critical commercial assays Advantage 2 Polymerase,TaKaRa

(9) Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomesCell Reports MedicineJanuary 16, 2024Ted Ling-Hu, Lacy M. Simons, Taylor J. Dean, Estefany Rios-Guzman, Matthew T. Caputo, Arghavan Alisoltani, Chao Qi, Michael Malczynski, Timothy Blanke, Lawrence J. Jennings, et al.,Thermo Scientific,N8080127 RNaseOUT Recombinant Ribonuclease Inhibitor,Thermo Scientific,10777019 dNTP Mix,Thermo Scientific,R0194 Q5 Hot Start High-Fidelity DNA Polymerase - 500 units,NEB,M0493L PhiX Control v3,Illumina,FC-110-3001 AmpureXP

(10) The Atad5 RFC-like complex is the major unloader of proliferating cell nuclear antigen in Xenopus egg extractsJournal of Biological ChemistryJanuary 1, 2024Yoshitaka Kawasoe, Sakiko Shimokawa, Peter J. Gillespie, J. Julian Blow, Toshiki Tsurimoto, Tatsuro S. Takahashi1 mM EDTA) containing 10 μg/ml RNase A. DNA samples were digested with XmnI (New England Biolabs, #R0194) and analyzed by 0.8% agarose gel electrophoresis followed by staining with SYBR Gold nucleic acid gel

(11) TAR Syndrome-associated Rbm8a deficiency causes hematopoietic defects and attenuates Wnt/PCP signalingbioRxivMay 5, 2023Agnese Kocere, Elena Chiavacci, Kevin Manuel Méndez-Acevedo, Charlotte Soneson, Max S. Hiltabidle, Azhwar Raghunath, Jacalyn S. MacGowan, Jordan A. Shavit, Daniela Panáková, Margot L. K. Williams, et al.Additionally, the rbm8a wildtype allele was genotyped by restriction digest of the PCR product with Xmn1 (R0194S, NEB) and the rbm8aΔ3 allele by Hinf1 (R0155S, NEB) while the rbm8aΔ5 allele stayed intact upon Xmn1

(12) RTEL1 and MCM10 overcome topological stress during vertebrate replication terminationCell ReportsFebruary 17, 2023Lillian V. Campos, Sabrina X. Van Ravenstein, Emma J. Vontalge, Briana H. Greer, Darren R. Heintzman, Tamar Kavlashvili, W. Hayes McDonald, Kristie Lindsey Rose, Brandt F. Eichman, James M. DewarProtein A,Invitrogen,10001D Dynabeads M280 Streptavidin,Invitrogen,11205D XmnI,New England Biolabs,R0194 Nb.BssSI,New England Biolabs,R0681 T4 Polynucleotide Kinase,New England Biolabs,M0201 ATP,Sigma,A7699 BSA,Sigma,A7906 Phosphocreatine,Sigma,P7936 DTT,Bio-Rad,1610611 Creatine

(13) Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombinationMolecular CellOctober 1, 2021Jasvinder S. Ahuja, Catherine S. Harvey, David L. Wheeler, Michael LichtenAldrich,3115879001 RNAse A,Sigma Aldrich,11119915001 Zymolyase,MP Biomedicals,08320932 XmnI,New England Biolabs,R0194L SpeI-HF,New England Biolabs,R3133L XcmI,New England Biolabs,R0533L HaeIII,New England Biolabs,R0108L NdeI,New England Biolabs

(14) Highly efficient manipulation of nervous system gene expression with NEPTUNECell Rep MethodsAugust 23, 2021Mangold K, Mašek J, He J, Lendahl U, Fuchs E, Andersson ER.Scientific,BP742 EcoRI-HF,NEB,R3101L SmaI,NEB,R0141S BamHI-HF,NEB,R3136L NheI,NEB,R3131S FseI,NEB,R0588S XmnI,NEB,R0194S SacII Enzyme,NEB,R0157 SphI-HF,NEB,R3182 NdeI,NEB,R0111S SalI-HF,NEB,R3138S CutSmart Buffer,NEB,B7204S Calf

(15) Signaling levels mold the RAS mutation tropism of urethaneeLifeMay 17, 2021Siqi Li, Christopher M Counterprotein EcoRI New England Biolabs Cat# R3101 Peptide, recombinant protein XmnI New England Biolabs Cat# R0194 Peptide, recombinant protein Exonuclease I New England Biolabs Cat# M0293 Commercial assay or kit iScript cDNA synthesis kit

(16) Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequencesPlant CommunicationsMarch 8, 2021Simon Sretenovic, Desuo Yin, Adam Levav, Jeremy D. Selengut, Stephen M. Mount, Yiping Qi(Lowder et al., 2015) containing pco-SpyCas9 was enzymatically digested with XmnI (NEB, catalog no. R0194∗) and AatlI (NEB, catalog no. R0117∗). pYPQ166 (Addgene no. 109328) (Lowder et al., 2015) containing

(17) Telomerase biogenesis requires a novel Mex67 function and a cytoplasmic association with the Sm7 complexeLifeOctober 23, 2020Yulia Vasianovich, Emmanuel Bajon, Raymund J Wellingerml per sample) Peptide, recombinant protein XmnI (restriction enzyme) New England Biolabs Cat#: R0194L Peptide, recombinant protein AleI-v2 (restriction enzyme) New England Biolabs Cat#: R0685L Peptide, recombinant

(18) IQGAP1 Negatively Regulates HIV-1 Gag Trafficking and Virion ProductionCell reportsMarch 24, 2020Sabo Y, de Los Santos K, Goff SP.IQGAP1-XPR-res fragment (IDT; Table S1) with XhoI and XmnI restriction enzymes (NEB; Cat# R0146 and R0194) and ligating it into pEGFP-IQGAP1 plasmid that was digested using the same restriction enzymes. Untagged

(19) Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAsNature CommunicationsDecember 1, 2019Kale Kundert et al.modified pCR2.1 vector ~2.1 kb downstream of its XmnI site. The vector was then digested with XmnI (NEB R0194S) as follows: mix 43.5 μL ≈500 ng/μL miniprepped pCR2.1 DNA, 5.0 μL 10× CutSmart buffer, and 1.5 μL 20

(20) Topoisomerase II Is Crucial for Fork Convergence during Vertebrate Replication TerminationCell ReportsOctober 8, 2019Darren R. Heintzman, Lillian V. Campos, Jo Ann W. Byl, Neil Osheroff, James M. DewarStreptavidin,Invitrogen,11205D hTop2a (Topogen),Topogen,TG2000H-1 AlwNI,New England Biolabs,R0514 XmnI,New England Biolabs,R0194 Nt.BspQI,New England Biolabs,R0644 Nb.BssSI,New England Biolabs,R0681 Biotin,Sigma,B4501 T4 Polynucleotide Kinase

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XmnI

XmnI from New England Biolabs

Sequence

New England Biolabs

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XmnI from New England Biolabs

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