XhoI from Promega

Product Specs
ItemXhoI
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberR6165
Quantity10,000u
Concentration10u/µl

Sequence

C▼TCGA G
G AGCT▲C

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) LukS-PV targeting C5aR inhibits EMT in hepatocellular carcinoma via the BCL6/HDAC6/HSPD1 axisCommunications BiologyJanuary 30, 2026Pengsheng Ding, Lan Shi, Xuexue Xu, Bing Lu, Gan Liu, Yangyan Wang, Zhengchao Nie, Xiaofang Wang, Wenjiao Chang, Yuanyuan Dai, et al.recombinant LukS-PV-GFP, the LukS-PV sequence was digested with BamHI (Promega, #R6021) and XhoI (Promega, #R6161), then ligated into the pMD-18T vector (Takara, Tokyo, Japan) to produce pMD-18T-LukS-PV plasmids. After

(2) Toxin protein LukS-PV targeting complement receptor C5aR1 inhibits cell proliferation in hepatocellular carcinoma via the HDAC7–Wnt/β-catenin axisJournal of Biological ChemistryFebruary 1, 2025Lan Shi, Shanshan Zhang, Gan Liu, Zhengchao Nie, Pengsheng Ding, Wenjiao Chang, Yuanyuan Dai, Xiaoling Maamplified from PVL-positive S. aureus isolates by PCR. PCR products were digested with XhoI (catalog no.: R6161; Promega) and BamHI (catalog no.: R6021; Promega) and ligated into the pET28a vector. Recombinant LukS-PV

(3) Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cellsiScienceJuly 21, 2023Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. KilpatrickWizard SV Gel and PCR Clean-Up System,Promega (USA),Cat# A9281 BamHI enzyme,Promega (USA),Cat# R6021 XhoI enzyme,Promega (USA),Cat# R6161 DH5 alpha Chemically Competent cells,invitrogen,Cat# 18265-017 T4 DNA ligase,New England Biolabs,Cat#

(4) Diepoxybutane induces the p53‐dependent transactivation of the CCL4 gene that mediates apoptosis in exposed human lymphoblastsJournal of Biochemical and Molecular ToxicologyMay 1, 2023Akamu J. Ewunkem, Maya Deve, Scott H. Harrison, Perpetua M. MugandaThermo-sensitive alkaline phosphatase (Cat# M9110), and the restriction enzymes Kpn1 (Cat# R6341) and Xho1 (Cat# R6161) were obtained from the Promega Corporation. The T4 ligase DNA Ligation Kit (Catalog #203003) was obtained

(5) Implications of differential transcription start site selection on chronic myeloid leukemia and prostate cancer cell protein expressioniScienceDecember 22, 2022Surani AA, Spriggs KA, Ufer C, Polytarchou C, Montiel-Duarte C.Ampicillin sodium salt,Sigma-Aldrich,Cat#A9518 Glycogen,ThermoFisher,Cat#AM9510 NheI restriction endonuclease,Promega,Cat#R6501 XhoI restriction endonuclease,Promega,Cat#R6161 Taq DNA polymerase,Promega,Cat#M7841 Alkaline Calf Intestinal Phosphatase,Promega,Cat# M1821 T4 DNA Ligase

(6) Probing the binding of interleukin-23 to individual receptor components and the IL-23 heteromeric receptor complex in living cells using NanoBRETCell chemical biologyJanuary 20, 2022Lay CS, Bridges A, Goulding J, Briddon SJ, Soloviev Z, Craggs PD, Hill SJ.restriction enzyme,Promega Corporation,Cat# R6181 Xho1 restriction enzyme,Promega Corporation,Cat# R6161 T4 Ligase,New England BioLabs,Cat# M0202S DAM negative E. coli,Agilent Technologies,Cat# 200247 DH5

(7) miR-151 Affects Low-Temperature Tolerance of Penaeus vannamei by Modulating Autophagy Under Low-Temperature StressFrontiers in Cell and Developmental BiologyApril 9, 2021QingJian Liang, WenNa Dong, MuFei Ou, ZhongHua Li, Can Liu, FeiFei Wang, Yuan Liu, WeiNa Wang

(8) EphA2 super-enhancer promotes tumor progression by recruiting FOSL2 and TCF7L2 to activate the target gene EphA2Cell death & diseaseMarch 12, 2021Cui S, Wu Q, Liu M, Su M, Liu S, Shao L, Han X, He H.

(9) TGFβR-SMAD3 Signaling Induces Resistance to PARP Inhibitors in the Bone Marrow MicroenvironmentCell reportsOctober 6, 2020Le BV, Podszywalow-Bartnicka P, Maifrede S, Sullivan-Reed K, Nieborowska-Skorska M, Golovine K, Yao JC, Nejati R, Cai KQ, Caruso LB, et al.Pierce ECL Western Blotting Substrate,Thermo Fisher Scientific,Cat# 32106 I-SceI,Thermo Fisher Scientific,Cat# ER1771 XhoI,Promega,Cat# R6161 KpnI,Promega,Cat# R6341 MesenCult™ MSC Basal Medium (Human),StemCell Technologies,Cat# 05401 MesenCult MSC Stimulatory Supplement (Human

(10) A two-lane mechanism for selective biological ammonium transporteLifeJuly 14, 2020Gordon Williamson et al.protein",NeRh50(His)6,This study,,purified from E. coli C43 GT1000 cells "Peptide, recombinant protein",XhoI,Promega,Cat# - R6161, "Peptide, recombinant protein",BamHI,Promega,Cat# - R6021, Commercial assay or kit,Quikchange XL

(11) The Basis for Strain-Dependent Rat Aldehyde Dehydrogenase 1A7 (ALDH1A7) Gene ExpressionMolecular pharmacologyNovember 1, 2019Touloupi K, Küblbeck J, Magklara A, Molnár F, Reinisalo M, Konstandi M, Honkakoski P, Pappas P.

(12) Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-betaAgingOctober 3, 2019Pan CH, Chen CJ, Shih CM, Wang MF, Wang JY, Wu CH.pGL4.10[Luc2] plasmid (#E6651; Promega, Madison, WI, USA) using digestion of the restriction enzymes, XhoI (#R6161; Promega) and BglII (#R6081; Promega), to construct four complete reporter plasmids, pGL4.10[Luc2]-rPDGFR-β,

(13) Complex Formation between VEGFR2 and the β₂-AdrenoceptorCell chemical biologyJune 20, 2019Kilpatrick LE, Alcobia DC, White CW, Peach CJ, Glenn JR, Zimmerman K, Kondrashov A, Pfleger KDG, Ohana RF, Robers MB, et al."Promega Corporation (Wisconsin, USA)",Cat# R6081 XhoI restriction enzyme,"Promega Corporation (Wisconsin, USA)",Cat# R6161 XbaI restriction enzyme,"Promega Corporation (Wisconsin, USA)",Cat# R6181 KpnI restriction enzyme,"Promega Corporation

(14) Rhabdovirus-Inducible MicroRNA-210 Modulates Antiviral Innate Immune Response via Targeting STING/MITA in FishThe Journal of ImmunologyAugust 1, 2018Tianjun Xu, Qing Chu, Junxia Cui

(15) Suberanilohydroxamic acid prevents TGF-β1-induced COX-2 repression in human lung fibroblasts post-transcriptionally by TIA-1 downregulationBiochimica et biophysica acta. Gene regulatory mechanismsMay 1, 2018Pasini A, Brand OJ, Jenkins G, Knox AJ, Pang L.5′-CTCTCTCGAGTTTCCAACACAGTGTCGCAG-3′. 1 μg of purified PCR products was then digested using SacI (R6061, Promega) and XhoI (R6161, Promega) restriction enzymes following the manufacturer's instructions. Digestion was then checked

(16) Intraocular Penetration of a vNAR: In Vivo and In Vitro VEGF165 NeutralizationMarine DrugsMarch 31, 2018Tanya A. Camacho-Villegas, María Teresa Mata-González, Walter García-Ubbelohd, Linda Núñez-García, Carolina Elosua, Jorge F. Paniagua-Solis, Alexei F. Licea-NavarroDiego, CA, USA), with the cloned gene into the NdeI-XhoI sites (Promega (Madison, WI, USA), R6801 and R6161). The culture was incubated at 37 ◦ C overnight in liquid LB medium (Sigma, L3022) that contained 100

(17) Identification of Specific Inhibitors of Trypanosoma cruzi Malic Enzyme Isoforms by Target-Based HTSSLAS DiscoveryOctober 1, 2017Americo T. Ranzani, Cristina Nowicki, Shane R. Wilkinson, Artur T. Cordeiro

(18) The tumor-suppressive microRNA-23b/27b cluster regulates the MET oncogene in oral squamous cell carcinomaInternational Journal of OncologySeptember 1, 2016Ichiro Fukumoto, Keiichi Koshizuka, Toyoyuki Hanazawa, Naoko Kikkawa, Ryosuke Matsushita, Akira Kurozumi, Mayuko Kato, Atsushi Okato, Yoshitaka Okamoto, Naohiko Seki

(19) Functional expression of novel human and murine AKR1B genesChemico-biological interactionsMay 30, 2011Salabei JK, Li XP, Petrash JM, Bhatnagar A, Barski OA.respectively. 2. Materials and Methods 2.1. Materials Restriction enzymes Nde1 (catalog number; R6801), Xho1 (R6161) and Not1 (R 6431), and AMV reverse transcriptase (M510A) were obtained from Promega. pET28a (+) (69864-3)

(20) A New N-Terminal Recognition Domain in Caveolin-1 Interacts with Sterol Carrier Protein-2 (SCP-2)BiochemistryJuly 17, 2007Rebecca D. Parr, Gregory G. Martin, Heather A. Hostetler, Megan E. Schroeder, Kiran D. Mir, Ann B. Kier, Judith M. Ball, Friedhelm Schroeder

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XhoI

Description

Sequence

Promega

Citations (20)

Reviews

XhoI from Promega

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