XhoI from New England Biolabs

Product Specs
ItemXhoI
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR0146S
Concentration20 u/µL
Quantity5000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/TCGAG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilizationiScienceMay 15, 2026Kalina M. Świst-Rosowska, Reagan W. Ching, Birgit Koschorz, Carmen Galan, Bettina Engist, Thomas JenuweinP4333 puromycin dihydrochloride,Sigma-Aldrich,Cat#: P9620 XhoI restriction enzyme,New England Biolabs,Cat#: R0146S AflII restriction enzyme,New England Biolabs,Cat#: R05020S PvuI restriction enzyme,New England Biolabs,Cat#:

(2) The MAFG-AS1/G6PD axis reduces platinum sensitivity in colorectal cancer through pentose phosphate pathway activationJ Transl MedApril 1, 2026Jia Hui Kou, Yujie Yao, Wen Jin Li, Jiajie Zou, Luke Kong, Junqi Zheng, Yi Meng Yue, Man Man Han, Su Tang Guoknockdown vector digested with BsmBI (New England Biolabs; Cat#R0580S) and XhoI (New England Biolabs; Cat#R0146S), followed by ligation with T4 DNA ligase (Thermo Fisher Scientific; Cat#EL0014). Lentiviral particles

(3) Hemocyte-secreted Papilin bearing mucin-type O-glycans regulates peripodial stalk formation via epidermal JAK/STAT signaling in DrosophilaiScienceMarch 20, 2026Takashi J. Fuwa, Kazuyoshi Itoh, Tomomi Ichimiya, Yoshihiro Akimoto, Shoko Nishihara,New England Biolabs,Cat# R3101S KpnI-HF,New England Biolabs,Cat# R3142S XhoI,New England Biolabs,Cat# R0146S Cellfectin™ II Reagent,Thermo Fisher Scientific,Cat# 10362100 Sf-900TM II SFM medium,Thermo Fisher Scientific

(4) A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based constructMolecular Therapy OncologyMarch 19, 2026Alexandros Kourtesakis, Hiu Nam Hannah Chow, Eileen Bailey, Sandra Horschitz, Ammar Jabali, Rainer Will, Christoph Schifflers, Abigail K. Suwala, Hannah Rohdjess, Melissa Hahn, et al.CAAGCTACGCGTTTACTTGTACAGCTCGTCCATGCC-3′), purified as above, and cloned into the LFBBz construct after dual digestion with XhoI (#R0146S, New England Biolabs) and MluI. DH5α competent bacterial cell transformation, O/N culture, picking and

(5) p53-induced RNA-binding protein ZMAT3 inhibits transcription of a hexokinase to suppress mitochondrial respiration in human cancer cellseLifeMarch 17, 2026Ravi Kumar, Simon Couly, Bruna R Muys, Xiao Ling Li, Ioannis Grammatikakis, Ragini Singh, Mary Guest, Xinyu Wen, Wei Tang, Stefan Ambs, et al.Bioscience and included restriction sites for KpnI (NEB, Catalog no. R3142) and XhoI (NEB, Catalog no. R0146S) at 5′ and 3′ ends, respectively. Both the pGL4-Basic vector and insert DNA fragment were digested with

(6) DPEP2 suppresses hyperinflammation via metabolic reprogramming of macrophages in sepsisNature CommunicationsMarch 9, 2026Wenchen Luo, Wei Xu, Qimeng Yin, Jieqiong Song, Jie Wang, Yian Guan, Jian Huang, Jian Yu, Kefeng Zou, Danfeng Jin, et al.expression vector78 (Addgene, 125839) were digested with XbaI (R0145S, New England Biolabs) and XhoI (R0146S, New England Biolabs), purified, and ligated using T4 DNA ligase. Recombinant plasmids were transformed

(7) Armored macrophage-targeted CAR-T cells reset and reprogram the tumor microenvironment and control metastatic cancer growthCancer CellMarch 9, 2026Jaime Mateus-Tique, Ashwitha Lakshmi, Bhavya Singh, Rhea Iyer, Alfonso R. Sánchez-Paulete, Chiara Falcomatà, Matthew Lin, Gvantsa Pantsulaia, Alexander Tepper, Trung Nguyen, et al.heavy and light chain were cloned into aFOLR2.CAR vector using Nsil-HF (NEB #R3127S) and XhoI (NEB #R0146S) replacing aFOLR2 scFV by the TREM2 scFV light and heavy chains separated by a 4x GGGGS linker. A Myc-tag

(8) A Precision-Engineered DC-Targeting mRNA-LNP Neoantigen Vaccine Elicits Stronger T Cell Responses and Exhibits Superior Tumor ControlVaccines (Basel)March 5, 2026Qi Liu, Yan Liu, Jinwei Li, Si Huang, Zhiying Chen, Jia Li, Tao Wang, Peipei Zhou, Jiandong Huo, Dehua LiThe preparation was conducted through a linearization procedure employing the XhoI endonuclease (CAT.R0146S, New England Biolabs, Ipswich, MA, USA). The in vitro transcription technique was employed to synthesize

(9) Tumor metabolic adaptation induced by L-asparaginase reveals a vulnerability to PARP1/2 inhibitor in B-cell lymphomasNature CommunicationsFebruary 27, 2026Anaïs Aussel, Ivan Nemazanyy, Ashaina Vandenberghe, Pauline Meola, Noémie Dorat, Rachel Paul, Lama Habbouche, Laurie Exposito, Nicolas Nottet, Virginie Petrilli, et al.HpaI (R0105S, New England Biolabs) restriction enzymes. The pMIG vector was linearized using XhoI (R0146S, New England Biolabs) and HpaI restrictions enzymes to generate compatible cohesive ends. Digested pMIG

(10) Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell ReceptorsResearch SquareFebruary 18, 2026Funsho J. Ogunshola, Nishant K. Singh, Vincent Butty, Anurag R. Mishra, Zacharia Habte, Liza Vecchiarello, Ahmed Fahad, Kate B. Juergens, Sophia Cheever, Marius Allombert, et al.into the pET22b(+) vector (Cat. 69744–3, Novagen) between the NdeI (Cat. R0111S, NEB) and Xhol (Cat. R0146S, NEB) restriction enzyme sites. The transformed E. coli were induced for inclusion body expression by

(11) Structures of proteinase 3 and the CD177 receptor complex reveal a major autoantibody epitopeEMBO ReportsFebruary 17, 2026Céline Zheng-Gérard, Jana Joha, Maria Carrasquero, Kamel El Omari, Edward Lowe, Shirish Dubey, Simon J Draper, Yu-Chi Chang, Hsi-Hsien Lin, Alan D Salama, et al.,New England Biolabs,Cat.#0101S AgeI,New England Biolabs,Cat.#R0552S KpnI,New England Biolabs,Cat.#R0142M XhoI,New England Biolabs,Cat.#R0146S In-Fusion® HD Cloning kit,Takara Bio,Cat.#121416 Stellar competent cells,Takara Bio,Cat.#636763 lysogeny

(12) Structural insights into zinc piracy by Neisseria gonorrhoeae to overcome nutritional immunityStructureFebruary 5, 2026Pooneh Tavakoley Gheinani, Aloke Bera, Julie Stoudenmire-Saylor, Yi Lien, Simone A. Harrison, Alison Criss, Walter J. Chazin, Nicholas Noinaj, Cynthia Nau CornelissenBlocker-nonfat dry milk,BioRad,Cat# 1706404 XmaI,New England BioLabs,Cat# R0180S XhoI,New England BioLabs,Cat# R0146S PciI,New England BioLabs,Cat# R0655L TPEN,EMD Millipore,Cat# 616394 Isopropyl-β-D-thiogalactopyranoside

(13) Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell ReceptorsbioRxivFebruary 2, 2026Funsho J. Ogunshola, Nishant K. Singh, Vincent Butty, Anurag R. Mishra, Zacharia Habte, Liza Vecchiarello, Ahmed Fahad, Kate B. Juergens, Sophia Cheever, Marius Allombert, et al.into the pET22b(+) vector (Cat. 69744–3, Novagen) between the NdeI (Cat. R0111S, NEB) and Xhol (Cat. R0146S, NEB) restriction enzyme sites. The transformed E. coli were induced for inclusion body expression by

(14) Effect of prolonged voluntary wheel running on oxidative stress and defence mechanisms in cortex and hippocampus of healthy female ratsExperimental PhysiologyFebruary 1, 2026Camilla Myrup Holst, Iria Esperon‐Abril, Frederik Bryske Juhl, Jesper Emil Jakobsgaard, Jonas B. Kristiansen, Kristian Vissing, Tinna Stevnsnernanogram per microlitre DNA was incubated with 1U/µL XhoI (New England BioLabs, Ipswich, MA, USA, cat. no. R0146S) in 1xrCutSmart buffer (New England BioLabs, cat. no. B6004) at 37°C for 15 min followed by heat inactivation

(15) Dye-cycling DNA origami rotors for long-term tracking of transcription at base-pair resolutionbioRxivJanuary 28, 2026Amanda Wacker, Ryan J. Fantasia, Brian J. Tenner, Jerry Wu, Nicholas A. Monell, Pallav Kosuri25 Kit D4033) and eluted at 50 μL. Concentrated template DNA was digested overnight with XhoI (NEB R0146S) on a thermomixer set to 1000 rpm at 37°C. Then, DNA was column cleaned once again, eluted at 50 μL,

(16) Structural analyses and substrate profiling of PPEP-3 provide new insights into the molecular basis of Pro-Pro endopeptidase specificityiScienceJanuary 16, 2026Bart Claushuis, Fabian Wojtalla, Lisa Papenhagen, Robert A. Cordfunke, Arnoud H. de Ru, Hans C. van Leeuwen, Jeroen Corver, Paul J. Hensbergen, Ulrich BaumannNdeI,New England Biolabs,Catalog No R0111S Restriction endonuclease XhoI,New England Biolabs,Catalog No R0146S Restriction endonuclease DpnI,New England Biolabs,Catalog No R0176S Isopropyl-β-D-thiogalactopyranoside

(17) The mechanism of cell-cycle-dependent proteasomal degradation of archaeal ESCRT-III homolog CdvB in SulfolobusThe EMBO JournalJanuary 9, 2026Yin-Wei Kuo, Jovan Traparić, Sherman Foo, Buzz BaumAS,Sigma-Aldrich,N4517 Bortezomib,Abcam,Ab142123 DMSO,Sigma-Aldrich,D8418 L-Arabinose,Sigma-Aldrich,A3256 Gelzan CM (Gelrite),Sigma-Aldrich,G1910 NcoI-HF,New England Biolabs,R3193S XhoI,New England Biolabs,R0146S Gibson Assembly Master Mix,New England Biolabs,E2611S Gene Pulser/MicroPulser Electroporation Cuvettes,Bio-Rad,1652089 Q5 High-Fidelity DNA Polymerase

(18) Efficient Fluorescent Labeling of Human Trophoblast Stem Cells via a CRISPR/Cas9-Mediated Knock-In Approach in a Safe Harbor LocusBIO-PROTOCOLJanuary 5, 2026Hengshan Zhang, Jie Zhou, Morgan Orsolini, Anthony Zhao, Askar Takhirov, Danny J. SchustIn our work, we performed enzyme digestions by choosing Xho I (New England Biolabs, catalog number: R0146S) or Not I (New England Biolabs, catalog number: R0189S) restriction enzymes, which have unique cutting

(19) The exon junction complex coordinates the cotranscriptional inclusion of blocks of neighboring exonsGenes & DevelopmentJanuary 1, 2026Alexandra Bergfort, Jackson M. Gordon, Matthew R. Gazzara, Chuan-Tien Hung, Benhur Lee, Yoseph Barash, Karla M. NeugebauerPrimeSTAR Max DNA polymerase (Takara R045A). PCR product was digested with BamHI-HF (NEB R3136S) and XhoI (NEB R0146S), purified with QIAquick PCR and gel cleanup kit (Qiagen 28506), ligated with pCDNA5-FRT/TO (Thermo

(20) Annexin A2 stabilizes the endoplasmic reticulum and actin cytoskeleton and influences the formation of reovirus factoriesJournal of VirologyDecember 23, 2025Raquel Tenorio, Gwen M. Taylor, Ana Cayuela, C. O. S. Sorzano, Isabel Fernández de Castro, Sara Y. Fernández-Sánchez, Alexa N. Roth, Xayathed Somoulay, Gavin S. Treadaway, Terence S. Dermody, et al.untagged ANXA2 plasmids. PCR fragments were cloned into pcDNA3.1 using NheI (NEB, R3131S) and XhoI (NEB, R0146S). ANXA2 KO cells were either mock-transfected or transfected with engineered plasmids in combination

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5/2/2018Diagnostic Restriction Mapping Of The Plasmid Construct With XhoI Triveni MahendrappaI have designed and constructed a plasmid, pET20-cadA of size 6130 bp. After transforming this plasmid into E. coli BL21 cells, positive clones were selected and the plasmid was prepped. The prepped ... Read Review

XhoI

XhoI from New England Biolabs

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New England Biolabs

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XhoI from New England Biolabs

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