XhoI from New England Biolabs

Product Specs
ItemXhoI
CompanyNew England Biolabs
Price $317.00 Inquire
Catalog NumberR0146L
Concentration20 u/µL
Quantity25000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/TCGAG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Technical and biological sources of noise confound multiplexed enhancer AAV screeningNat CommunApril 28, 2026Avery C. Hunker, John K. Mich, Naz Taskin, Amy Torkelson, Trangthanh Cardenas, Jean-Benoît Lalanne, Joseph T. Mahoney, Darren Bertagnolli, Anish Bhaswanth Chakka, Rushil Chakrabarty, et al.of each enhancer plasmid resulting from the steps above was digested overnight with 1 µL XhoI (NEB R0146L) and 10x rCutsmart Buffer (NEB B6004S) diluted in water. Digested plasmids were cleaned by gel extraction

(2) Molecular basis of the short- and long-term osmoregulation capability in the euryhaline unicellular eukaryote Paramecium calkinsimBioApril 8, 2026Jia Liu, Juan Yang, Xue Zhang, Rui Wang, Lei Yang, Huan Dou, Rebecca A. Zufall, Xiao Chen, Feng Gaoplasmid extraction. A double restriction-enzyme digestion segment generated using XhoI (NEB, USA, Cat. No. R0146L) and SacII (NEB, USA, Cat. No. R0157L) from a pCE2 plasmid was ligated into the L4440 plasmid, which

(3) Preparation and application of YFV-17D-derived Anterograde Trans-neuronal Viral Vectors for Neuroscience ResearchbioRxivJanuary 13, 2026Tanvi Panchumarthy, Lizbeth Munoz, Uzair Saleem, Ai-Vy Le, Ian Nelson, Ying Li, Xiangmin Xu, Wei XumMESSAGE mMACHINE™ SP6 Transcription Kit (Life Technologies, Cat. No. AM1340)Restriction endonuclease Xho1 and buffer (NEB, Cat. No. R0146L)Buffer-saturated phenol/chloroform (Sigma, Cat. No. P2069-400ML)Chloroform (Fisher, Cat. No. AC383770010) Reagents

(4) A non-canonical ARMS-GABARAP interaction modulates dendritic spine formation and synaptic developmentThe EMBO JournalJanuary 8, 2026Wenli Jiang, Jin Ye, Jiasheng Chen, Xinyu Wang, Yahong Li, Jianchao Li, Yide Mei, Yanlu Lyu, Wei Hu, Chao Wangbuffer,Vazyme,P022-01 DNA marker,Vazyme,MD102-02 BamHI-HF,NEB,R3136L EcoRI-HF,NEB,R3101L Xhol,NEB,R0146L AgeI-HF,NEB,R3552S CutSmart,NEB,B7204S Proteinase K,MERCK,03115879001 Chloroquine,MCE,HY-17589A Trichloroacetic

(5) Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosisNucleic Acids ResearchJanuary 8, 2026Prakshi Gaur, Thejas Satheesh, Rohit Kumar Singh, Hussain Beig, Sneha Shahu, Saminathan Ramakrishnan, Mansi Srivastava, Shreyasi Neogi, Ayesha Dash, Amit Singh, et al.purified after gel electrophoresis, and the product was digested with NcoI (NEB R31935) and XhoI (NEB R0146L) restriction enzymes. The resulting product was then ligated with similarly digested pET-28a cloning

(6) CpoS-Inc interactions facilitate host cell modulation during Chlamydia trachomatis infectionInfection and ImmunityDecember 16, 2025Xavier Tijerina, C.A. Jabeena, Robert Faris, Zhen Xu, Parker Smith, Nicholas J. Schnicker, Mary M. Weberwas cloned into pGEX6P1 (Sigma Cat#GE28-9546-48) using BamHI-HF (NEB Cat#R3136L) and XhoI (NEB Cat#R0146L) to generate an N-terminal fusion to a GST-tag. All primers are listed in Table S1. The integrity of

(7) Replication competition drives the selective mtDNA inheritance in Drosophila ovaryCell ReportsSeptember 23, 2025Cheng Zhang, Zhe Chen, Hansong Ma, Hong Xu15140122 BSA,Sigma,Cat# A8806 Schneider’s Drosophila medium,Gibco,Cat# 21720 XhoI,New England Biolabs,Cat# R0146L BglII,New England Biolabs,Cat# R0144M Hi T4 DNA ligase,New England Biolabs,Cat# M2622L T4 DNA ligase,New England Biolabs

(8) A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapyCell Rep MethodsJuly 21, 2025Pengkai Sun, Bin Wang, Caiyun Liu, Zixiong Wang, Yang Liu, Yuan-Biao Qiao, Xinjian Liand recombinant proteins" ,, BamH I,New England Biolabs,Cat#R3136T Xho I,New England Biolabs,Cat#R0146L BsmB I,New England Biolabs,Cat#R0739S NaCl,Sigma-Aldrich,Cat#S9888 Dithiothreitol (DTT),Sigma-Aldrich,Cat#D9779 phenylmethanesulfonyl

(9) Protocol for engineering poly(ethylene terephthalate) hydrolases via directed evolution using a high-throughput screening assaySTAR ProtocJuly 15, 2025Thomas M. Groseclose, Zoe K. Taylor, Lexy A. Lujan, Taraka Dale, Hau B. Nguyen,New England Biolabs,Cat#B7204S T4 DNA ligase + buffer,New England Biolabs,Cat#M0202L XhoI Enzyme,New England Biolabs,Cat#R0146L Phosphoric acid for HPLC,Supelco,Cat#49685-100ML BamHI Enzyme,New England Biolabs,Cat# R0136L NdeI

(10) Protocol for CRISPR-mediated deletion of cis-regulatory element in murine Th17 cells for in vivo assessment of effector functionSTAR ProtocolsJune 20, 2025Xiancai Zhong, Hongmin Wu, Guanpeng Wang, Zuoming Sun,New England Biolabs,Cat# R3539L EcoRI-HF,New England Biolabs,Cat# R3101S XhoI,New England Biolabs,Cat# R0146L T4 DNA ligase,New England Biolabs,Cat# M0202S T4 DNA ligase buffer,New England Biolabs,Cat# B0202S MEM

(11) Stem loop binding protein promotes SARS-CoV-2 replication via -1 programmed ribosomal frameshiftingSignal Transduct Target TherJune 13, 2025Tanxiu Chen, Ruimin Zhu, Tingfu Du, Hao Yang, Xintian Zhang, Zhixing Wang, Yong Zhang, Wenqi Quan, Bin Yin, Yunpeng Liu, et al.°C. In vitro translation The pcDNA4.0 SARS-CoV-2 frameshift reporter vector was linearized by XhoI (#R0146L, NEB) as the DNA template. The linearized DNA template was transcribed and translated with the TNT® T7 Coupled Reticulocyte Lysate System

(12) Redox regulation of G protein oligomerization and signaling by the glutaredoxin WG1 controls grain size in riceThe EMBO JournalMay 19, 2025Lijie Liu, Jianqin Hao, Ke Huang, Penggen Duan, Baolan Zhang, Zhihai Chi, Xiaohong Yao, Yunhai Li,New England Biolabs,R3138S Not I,New England Biolabs,R3189S Hind III,New England Biolabs,R3104L Xho I,New England Biolabs,R0146L BstB I,New England Biolabs,R0519V Spe I,New England Biolabs,R3133S Bgl I,New England Biolabs,R0143S MES,GPCSCI,AN443 MgCl2,Sigma,M2393 Acetosyringone,Macklin,A800901 Isopropyl

(13) Protocol for producing brain-derived neurotrophic factor and neurotrophin-4 in their pro and active form in Escherichia coliSTAR ProtocMarch 28, 2025Christoph Holzner, Katharina Böttinger, Christian G. Huber, Elfriede Dall, Hans Brandstetter,Thermo Fisher Scientific,Cat#88222 Chloramphenicol,Sigma-Aldrich,Cat#C0378 Ampicillin,Sigma-Aldrich,Cat#A9393 DNase 1,Thermo Fisher Scientific,Cat#EN0525 Xho 1,New England Biolabs,Cat#R0146L Nde 1,New England Biolabs,Cat# R0111S Furin,This lab, Trypsin,Sigma-Aldrich,Cat#T6567 ,, Critical

(14) A highly stable lyophilized mRNA vaccine for Herpes Zoster provides potent cellular and humoral responsesNPJ VaccinesMarch 14, 2025Raquel Munoz-Moreno, Viola Allaj, Eddie Gadee, Julie M. Button, Fernando Diaz, Mohan S. Maddur, Wei Chen, Cheng Hui Hu, Lyndsey Martinez, Andreas Giannakou, et al.Builder HiFi DNA Assembly NC0825276) or standard restriction digestion with SpeI (NEB, R3133L) and XhoI (R0146L) followed by ligation (Promega, PR-M1794) into our proprietary linearized vector were performed45,46. Vector

(15) p53 regulates DREAM complex-mediated repression in a p21-independent mannerThe EMBO JournalMarch 4, 2025Ritu Agrawal, Sagar SenguptaBamHI HF,New England BioLabs,Cat# R0136L SacI,New England BioLabs,Cat# R3156L XhoI,New England BioLabs,Cat# R0146L GST,This study,N/A GST p53 (1-393),This study,N/A GST p53 (1-75),This study,N/A GST p53 (76-320),This

(16) Targeted C-to-T Base Editing in the Arabidopsis Plastid GenomeCurr ProtocJanuary 6, 2025Issei Nakazato, Shin-Ichi Arimura(New England Biolabs, cat. no. R0558S)",0.2 µl Restriction enzyme 2,0.2 µl,"XhoI (New England Biolabs, cat. no. R0146L)",0.2 µl,"EcoRI‐HF (New England Biolabs, cat. no. R3101L)",0.2 µl "Sterile distilled water (e.g., Nippon

(17) Myoblast-derived ADAMTS-like 2 promotes skeletal muscle regeneration after injurynpj Regenerative MedicineDecember 19, 2024Nandaraj Taye, Levon Rodriguez, James C. Iatridis, Woojin M. Han, Dirk Hubmacherwhich contains a signal peptide for secretion, were digested with BamHI (NEB #R3136T) and XhoI (NEB #R0146L) and ligated with T4 DNA ligase (NEB #M0202S) at 16 °C overnight. The ligated product was transformed

(18) FB5P-seq-mAbs: monoclonal antibody production from FB5P-seq libraries for integrative single-cell analysis of B cellsFrontiers in ImmunologyDecember 17, 2024Sakina Ado, Chuang Dong, Noudjoud Attaf, Myriam Moussa, Agathe Carrier, Pierre Milpied, Jean-Marc NavarroTween 20,Merck,cat.no. P9416-100ML Valproic acid,Merck,cat.no. V0033000 XhoI,New England Biolabs,cat.no. R0146L Yeast extract,Merck,cat.no. Y1625-250G

(19) Macrophages are activated toward phagocytic lymphoma cell clearance by pentose phosphate pathway inhibitionCell reports. MedicineDecember 17, 2024Beielstein AC, Izquierdo E, Blakemore S, Nickel N, Michalik M, Chawan S, Brinker R, Bartel HH, Vorholt D, Albert L, et al.EcoR1,New England Biolabs Inc.,Cat#R0101L Restriction Endonuclease Xho1,New England Biolabs Inc.,Cat#R0146L Succinic acid D6,SIGMA-ALDRICH,Cat#488356 Vent polymerase,New England Biolabs Inc.,Cat#M0245S ,, Critical

(20) Identification of suitable target/E3 ligase pairs for PROTAC development using a rapamycin-induced proximity assay (RiPA)eLifeDecember 6, 2024Bikash Adhikari, Katharina Schneider, Mathias Diebold, Christoph Sotriffer, Elmar Wolfprotein",SpeI-HF,New England BioLabs,Cat# R3133L, "Peptide, recombinant protein",XhoI,New England BioLabs,Cat# R0146L, Commercial assay or kit,Nano-Glo Luciferase Assay,Promega,Cat# N1120, Commercial assay or kit,GeneJET Gel Extraction Kit

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XhoI

XhoI from New England Biolabs

Sequence

New England Biolabs

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XhoI from New England Biolabs

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