pUC19 Vector from New England Biolabs

Product Specs
ItempUC19 Vector
CompanyNew England Biolabs
Price $329.00 Inquire
Catalog NumberN3041L
TypeVector
HostE. coli
ApplicationsDNA Manipulation
Copy NumberHigh Copy Number
MCSApo I - EcoR I 396, Ban II - Sac I 402, Acc65 I - Kpn I 408, Ava I - BsoB I - Sma I - Xma, BamH I 417, Xba I 423, Acc I - Hinc II - Sal I 429, BspM I - BfuA I 433, Sbf I 434, Pst I 435, Sph I 441and Hind III 447
Vector NamepUC19
Origin of ReplicationColE1
Quantity250 µg
ResistanceAmpicillin
Temperature-20 C
Selectable MarkerAmpicillin

Specifications/Features

Kit Contents:
250 ug pUC19

Features:
Features by Location:
* 469- 146 lacZ alpha CDS (start 469, complementary strand)
* 519- 514 Plac promoter -10 sequence (TATGTT)
*543- 538 Plac promoter -35 sequence (TTTACA)
* 575- 563 CAP protein binding site
* 396- 452 multiple cloning site (EcoRI-HindIII)
* 1455- 867 origin of replication (counterclockwise) (RNAII -35 to RNA/DNA switch point):
*1273-1278 RNAI transcript promoter -35 sequence (TTGAAG)
*1295-1300 RNAI transcript promoter -10 sequence (GCTACA)
* 1309-1416 RNAI transcript
* 1419- 867 RNAII transcript (complementary strand)
* 1434-1429 RNAII transcript promoter -10 sequence (CGTAAT)
* 1455-1450 RNAII transcript promoter -35 sequence (TTGAGA)
* 2486-1626 beta-lactamase (bla; amp-r) CDS (start 2486, complementary strand)
* 2486-2418 beta-lactamase signal peptide CDS (start 2486, complementary strand)
* 2521 bla RNA transcript start (complementary strand)
*2535-2530 bla promoter -10 sequence (GAGACA)
* 2556-2551 bla promoter -35 sequence (TTCAAA)

Vector Size:
2686 bp

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Magnetically Localized Detection of Amplified DNA Using Biotinylated and Fluorescent Primers and Magnetic NanoparticlesBiosensors (Basel)March 18, 2025Etienne Orsini, Franz Bruckert, Marianne Weidenhaupt, Orphée Cugat, Paul Kauffmann, Sarah DelshadiKit (DNA and RNA) (E1700L) solution containing a LAMP Fluorescent Dye and the plasmid target pUC19 (N3041L) were provided by New England Biolabs. The six primers were designed with the New England Biolabs LAMP

(2) Curcumin inhibits the activity of ubiquitin ligase Smurf2 to promote NLRP3‑dependent pyroptosis in non‑small cell lung cancer cellsInt J OncolFebruary 13, 2025Yunzhu Xi, Saili Zeng, Xiaowu Tan, Xiaoyu Dengoverexpression plasmid (oe-Smurf2), respectively. The plasmid backbone of oe-Smurf2 was pUC19 (cat. no. N3041L; New England BioLabs, Inc.). For the oe-Smurf2 + MG132 group, cells were first transfected with oe-Smurf2

(3) LAMP-Seq: Sensitive, Scalable, and Multiplexable COVID-19 Diagnostics Using Molecular BarcodingNature BiotechnologyDecember 1, 2021Kerstin U. Ludwig et al.primer (ACCTTCGGGAACGTGGTT; 100 µM, IDT) j. 80 µl of pUC19 plasmid DNA (1 µg µl−1, New England Biolabs N3041L) k. 12.16 ml of water l. 3.2 ml of wild-type Bst-LF-Idaho polymerase (1 mg ml−1). 25-µl LAMP-Seq reactions

(4) Structured elements drive extensive circular RNA translationMolecular cellOctober 21, 2021Chen CK, Cheng R, Demeter J, Chen J, Weingarten-Gabbay S, Jiang L, Snyder MP, Weissman JS, Segal E, Jackson PK, et al.Sense,Addgene,Cat# 60631 Plasmid: pHAGE-FGFR1,Addgene,Cat# 116740 Plasmid: pUC19 Vector,New England Biolabs,Cat# N3041L Plasmid: pLV-mCherry,Addgene,Cat# 36084 Plasmid: mRuby-ZKSCAN-split-eGFP reporter,This paper,N/A Plasmid:

(5) In vitro Assays for Eukaryotic Leading/Lagging Strand DNA ReplicationBIO-PROTOCOLSeptember 20, 2017Grant Schauer, Jeff Finkelstein, Mike O’DonnellGeorgescu et al. [2014] for purification details) pUC19, 1 mg/ml (New England Biolabs, catalog number: N3041L) BsaI-HF with CutSmart buffer (New England Biolabs, catalog number: R3535L) ‘blockLd’ oligo* ‘blockLg’

(6) The phosphate clamp: sequence selective nucleic acid binding profiles and conformational induction of endonuclease inhibition by cationic Triplatin complexesNucleic Acids ResearchDecember 16, 2014Andreea Prisecaru, Zara Molphy, Ralph G. Kipping, Erica J. Peterson, Yun Qu, Andrew Kellett, Nicholas P. Farrelland transfer RNA (tRNA, AM7119) was supplied from Invitrogen, supercoiled plasmid DNA (pUC19, Cat. N3041L) and restriction endonucleases AatII (R0117 S; 20 000 U/ml), BamHI (R0136 S; 20 000 U/ml), EcoRI (R0101

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pUC19 Vector

pUC19 Vector from New England Biolabs

Description

Specifications/Features

New England Biolabs

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Reviews

pUC19 Vector from New England Biolabs

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