pGL4.23[luc2/minP] Vector from Promega

Product Specs
ItempGL4.23[luc2/minP] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE8411
HostE. Coli, Mammalian
Reporterluc2
Vector NamepGL4.23[luc2/minP]
MCSAcc65I, KpnI, EcoICRI, SacI, NheI, BmtI, XhoI, EcoRV, Bgl II, HindIII
Quantity20microg
Origin of ReplicationColE1-derived
ResistanceAmpicillin
Molecular Weight~71kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4283bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Cellular Functional Analyses of ARX Variants Reveal New Insights Into Genotype-Phenotype Correlations in Neurodevelopmental Disorders Among Male and Female PatientsHum MutatApril 7, 2026Rasha Faraj, Audrey Farrugia, Anna C. E. Hurst, Pierre Conan, Jennifer Martin, Audrey Schalk, Sylvia Redon, Aline Dubos, Mathilde Gras, Aurore Curie, et al.from mouse genomic DNA and subsequently cloned into the pGL4.23 firefly luciferase vector (Promega, E8411) using the In‐Fusion cloning strategy (Clontech) and primers listed in the supporting data (mLmo1‐F

(2) Profiling the epigenomic landscape of late embryonic and adult mouse hind limb musclesScientific ReportsFebruary 17, 2026Samantha R. Queeno, Alexander S. Okamoto, Damien M. Callahan, Matthew C. O’Neill, Terence D. Capellini, Kirstin N. Sternerupstream of the firefly luciferase reporter gene (luc2) promoter in the pGL4.23 [luc2/minP] vector (E8411, Promega) by Azenta Life Sciences. All reporter vector constructs were verified by Sanger sequencing120.

(3) LDB1 represses fetal hemoglobin expression by enhancing BCL11A transcriptionRedox BiolFebruary 4, 2026Si-Won Park, Chang-Yong Choi, In-Byung Park, Seok-Jin Kang, Hyeon Jeong Lee, Ji-In Kim, Joonbeom Bae, Dongho Geum, Yong-Pil Cheon, Taehoon Chunthe fourth intron (Bcl11a intron #4) at the Bcl11a locus were cloned into a pGL4.23 vector (Promega E8411) using BamHI and SalI restriction sites to enhance the mRNA expression of Luc2 firefly luciferase (Fig.

(4) Single-cell multiome and enhancer connectome of human retinal pigment epithelium and choroid nominate causal variants in macular degenerationCell ReportsJanuary 27, 2026Sean K. Wang, Jiaying Li, Surag Nair, Reshma Kosaraju, Yun Chen, Yuanyuan Zhang, Anshul Kundaje, Yuwen Liu, Ningli Wang, Howard Y. ChangDNA hSTARR-seq_ORI vector,Muerdter et al.76,Addgene Cat# 99296 pGL4.23[luc2/minP] vector,Promega,Cat# E8411 Software and algorithms,Software and algorithms,Software and algorithms Cell Ranger-ARC,10x Genomics,v2.0.0 ArchR,Granja

(5) Global Evaluation of Congenital Heart Disease-Associated Non-Coding VariantsResearch SquareJanuary 7, 2026Edwin G. Rodríguez-Martínez, Shreya Peña-Martínez, Joshua G. Medina-Feliciano, Joshua Medina-Feliciano, Lois G. Parks, Marissa Granitto, Diego A. Pomales-Matos, Jean L. Pomales-Matos, Jean Messon- Barreiro-Rosario, Adriana Barreiro-Rosario, et al.cloned into a previously generated pGL4.23ΔxbaΔluc empty vector from pGL4.23 [luc2/minP] (Promega, # E8411). First, 20 bps barcodes were added to the synthesized oligos through 24X PCR with 50 μL system, each

(6) Multi-omics dissection of the genetic regulation underlying fatty acid composition in cattleGenome BiolDecember 22, 2025Tianliu Zhang, Qunhao Niu, Tianzhen Wang, Jiayuan Wu, Bo Zhu, Zezhao Wang, Xue Gao, Yan Chen, Huijiang Gao, Lupei Zhang, et al.with KpnI and HindIII restriction enzymes before inserted into PGL4.23(Luc2-minP) vectors (Promega, E8411). For dual-luciferase reporter assays, 293T cells were plated in 12-well plates and transfected with

(7) Functional and Computational Interrogation of the Juvenile Idiopathic Arthritis Risk Loci Identifies Candidate Causal SNPs and Target Genes in CD4+ T cellsmedRxivDecember 16, 2025Kaiyu Jiang, Emma K. Haley, Gilad Barshad, Adam He, Anita Rogic, Edward J. Rice, Marc Sudman, Susan D Thompson, Charles G. Danko, James N. JarvisXhoI and BglII (New England Biolabs, MA, USA) and ligated into a pGL4.23[luc2/minP] vector (Promega E8411, Madison, MI, USA), previously digested using the same endonucleases. The ligation mixtures were transformed

(8) Hepatic metabolic reprogramming in male mice during short-term caloric restriction involves enhanced glucocorticoid rhythmsNat CommunDecember 11, 2025Konstantinos Makris, Vlera Fonda, Fania Feby Ramadhani, Lina Fadel, Morgane Davezac, Bertrand Payet, Ioannis K. Deligiannis, Liwei Zhang, Teresa Horn, Laura Heimerl, et al.assays Luciferase reporters were generated by cloning cis-regulatory elements into pGL4.23 (Promega, #E8411). Mutagenesis of the putative FOXO1 binding sites was performed using the Q5 site-directed mutagenesis kit

(9) IP-SNPs-seq links noncoding risk alleles to lineage transcription factor programs in prostate cancerCell Mol Life SciDecember 8, 2025Wenjie Xu, Qixiang Zhang, Lijuan Qiao, Zixi Wang, Tian Wang, Dandan Dong, Qin Zhang, Liang Wang, Gong-Hong Wei, Peng Zhang(ODC1), rs3800285 (FOXP4), and rs902774 (KRT8) were cloned into firefly luciferase pGL4.23-minP vectors (E8411, Promega). Constructs were transfected into 22Rv1 and LNCaP cells using Lipofectamine 3000 DNA Transfection

(10) Combined SNPs sequencing and allele specific proteomics capture reveal functional causality underpinning the 2p25 prostate cancer susceptibility locusNat CommunOctober 8, 2025Dandan Dong, Zixian Wang, Mengqi Liu, Qin Zhang, Wenjie Xu, Yu Wei, Jing Zhu, Xiayun Yang, Qixiang Zhang, Yao Zhu, et al.DNA of human prostate cancer cells into a firefly luciferase pGL4.23 minimal promoter (minP) vector (#E8411, Promega) or the pGL3 SV40 promoter vector (#E1761, Promega) to assess enhancer activity. The constructs

(11) Super‐Enhancer‐Mediated DLX5 Activation Defines Regulatory Mechanisms in Human Embryonic Stem Cell‐Derived OsteoblastsDevelopment, Growth & DifferentiationSeptember 2, 2025Shinse Fujita, Shoichiro Tani, Hiroyuki Okada, Taku Saito, Sakae Tanaka, Shinsuke Ohba, Ung‐il Chung, Hironori HojoKpnI and XhoI sites was subcloned into the multiple cloning site of the pGL4.23 luciferase vector (E8411; Promega, Madison, USA) to make the Human DLX5 DE pGL4.23 luciferase vector. The AscI‐PacI‐Human CTCF

(12) Integrative single-cell multi-omics profiling of human pancreatic islets identifies T1D-associated genes and regulatory signalsCell reportsAugust 26, 2025D'Oliveira Albanus R, Zhang X, Zhao Z, Taylor HJ, Tang X, Han Y, Orchard P, Varshney A, Zhang T, Manickam N, et al.DNA,Recombinant DNA ,, pX330-U6-Chimeric_BB-CBh-hSpCas9,Addgene,Cat#42230 Pgl4.23[luc2/minP],Promega,Cat#E8411 phRL-SV40,Promega,Cat#E6261 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, FlowJo,FLOWJO,https://www.flowjo.com/ GraphPad

(13) Multimodal analyses reveal genes driving electrophysiological maturation of neurons in the primate prefrontal cortexNeuronAugust 6, 2025Yu Gao, Qiping Dong, Kalpana Hanthanan Arachchilage, Ryan D. Risgaard, Moosa Syed, Jie Sheng, Danielle K. Schmidt, Ting Jin, Shuang Liu, Soraya O. Sandoval, et al.adjacent NFIA ATAC-seq peak, was cloned into a pGL4.23[luc2 minP] firefly luciferase vector (Promega, E8411) using XhoI and HindIII restriction sites. To generate the first MEIS2 binding motif mutation construct,

(14) The human genetic variant rs6190 unveils Foxc1 and Arid5a as novel prometabolic targets of the glucocorticoid receptor in muscleSci AdvJuly 11, 2025Ashok Daniel Prabakaran, Fabian Montecino-Morales, Kevin McFarland, Thirupugal Govindarajan, Hima Bindu Durumutla, Hannah Latimer, Olukunle Akinborewa, Chiara Villa, Douglas P. Millay, Mattia Quattrocelliplasmids containing regulatory fragments were obtained cloning genomic sequences in the pGL4.23 backbone (#E8411, Promega), conserving the genomic orientation of the target sequences with regard to transcriptional

(15) Protocol for evaluating the impact of non-coding variants on transcription factor binding and gene expressionSTAR ProtocJune 20, 2025Edwin G. Peña-Martínez, Jessica M. Rodríguez-Ríos, Jean L. Messon-Bird, Adriana C. Barreiro-Rosario, Rosalba Velázquez-Roig, Alejandro Rivera-Madera, Esther A. Peterson-Peguero, José A. Rodríguez-MartínezDNA,Recombinant DNA ,, pET-28a(+)-GATA4,Twist Bioscience,N/A pGL4.23 [Luc2/minP - firefly luciferase],Promega,E8411 pNL1.1.CMV [Nluc/CMV] Vector,Promega,N1091 GATA4 pTwist CMV BG WPRE Neo,Twist Bioscience,N/A ,, Software

(16) Shared loci but distinct variants underlie genetic architecture of allergic diseasesmedRxivJune 7, 2025Isabella M. Salamone, Xiaoyuan Zhong, Noboru J. Sakabe, Robert Mitchell, William Wentworth-Sheilds, Li Zhang, Emma E. Thompson, Donna C. Decker, Clayton H. Rische, Xuanyao Liu, et al.variant(s) of interest. Sequences were ordered from IDT and cloned into the pGL4.23 plasmid (Promega E8411) using Gibson Assembly Master Mix (NEB E2611). 16HBE cells were transfected using Lipofectamine LTX reagent

(17) A Sox2 enhancer cluster regulates region-specific neural fates from mouse embryonic stem cellsG3: Genes, Genomes, GeneticsApril 17, 2025Ian C Tobias, Sakthi D Moorthy, Virlana M Shchuka, Lida Langroudi, Mariia Cherednychenko, Zoe E Gillespie, Andrew G Duncan, Ruxiao Tian, Natalia A Gajewska, Raphaël B Di Roberto, et al.(Thermo Fisher L3000008) with pSox2-pGL4.23 reporter vectors and pGL4.75 encoding Renilla luciferase (Promega E8411 and E6931) at a 50:1 molar ratio. The Renilla plasmid served as an internal control for transfection efficiency

(18) Amplified dosage of the NKX2-1 lineage transcription factor controls its oncogenic role in lung adenocarcinomaMolecular CellApril 3, 2025John L. Pulice, Matthew MeyersonLab,RRID:Addgene_12260 pCMV-VSV-G,"Stewart et al., (2003)111",RRID:Addgene_8454 pGL4.23,Promega,CAT#E8411 pRL-CMV,Promega,CAT#E2261 pLenti-dCas9-KRAB-MeCP2-Blast,"Liu et al., 202199",RRID:Addgene_221501 lentiGuide-Puro,"Sanjana

(19) High-quality sika deer omics data and integrative analysis reveal genic and cellular regulation of antler regenerationGenome ResearchJanuary 22, 2025Zihe Li, Ziyu Xu, Lei Zhu, Tao Qin, Jinrui Ma, Zhanying Feng, Huishan Yue, Qing Guan, Botong Zhou, Ge Han, et al.reporter constructs engineered in this study were developed on the pGL4.23[luc2/minP] vector (Promega E8411) and the pGL4.74[hRluc/TK] vector (Promega E6911), which served as the base plasmid. The Dual-Glo Luciferase

(20) OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expressionFront Plant SciJanuary 9, 2025Win Tun, Kieu Thi Xuan Vo, Behnam Derakhshani, Jinmi Yoon, Lae-Hyeon Cho, Kay Tha Ye Soe Win, Sang-Won Lee, Ki-Hong Jung, Jong-Seong Jeon, Gynheung Ansubcloned into a pGL4.23 vector that consisted of a minimal promoter and the Luciferase coding sequence (E8411, Promega). To normalize luciferase activity, the ZmUbi : GUS vector (Cho et al., 2008) was used as an

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