pGL4.22[luc2CP/Puro] Vector from Promega

Product Specs
ItempGL4.22[luc2CP/Puro] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6771
HostMammalian
Quantity20microg
Reportersynthetic firefly luciferase gene, luc2
Vector NamepGL4.22[luc2CP/Puro] Vector
ResistancePuromycin
PromoterPromoterless
MCSBgl I/Sfi I
Acc65 I
Kpn I
EcoICR I
Sac I
Nhe I
Xho I
EcoR V
Bgl II
Bgl I/Sfi I
Hind III
Origin of ReplicationColEI-derived plasmid replication origin
Molecular Weight~49kDa

Specifications/Features

Vector Size:
5583 bp

Features:
The following features are available for the pGL4 Vectors:

• two reporter genes: a choice of firefly luciferase (luc2) or Renilla luciferase (hRluc)
• reporter genes that code for stable or destabilized proteins
• basic vectors without promoters
• vectors with promoters such as HSV-TK, SV40 and CMV
• mammalian selectable marker for hygromycin, neomycin and puromycin resistance

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) ER stress upregulates S100A11 in steatohepatitis models via epigenetic modifications within the lipotoxicity-influenced enhancerJournal of Clinical InvestigationDecember 1, 2025P. Vineeth Daniel, Hanna L. Erickson, Daheui Choi, Feda H. Hamdan, Yasuhiko Nakao, Gyanendra Puri, Takahito Nishihara, Yeriel Yoon, Amy S. Mauer, Debanjali Dasgupta, et al.800 bp of the human S100A11 promoter upstream of the TSS was cloned into pGL4.22 plasmid (Promega, E6771) following conventional restriction digestion and ligation method. The XBP1s site was further mutated

(2) H1-0 is a specific mediator of the repressive ETV6::RUNX1 transcriptional landscape in preleukemia and B cell acute lymphoblastic leukemiaHemasphereApril 2, 2025Vera H. Jepsen, Andrea Hanel, Daniel Picard, Rigveda Bhave, Rebecca Hasselmann, Juha Mehtonen, Julian Schliehe-Diecks, Carla-Johanna Kath, Vithusan Suppiyar, Yash Prasad, et al.PCR‐amplified from REH cell genomic DNA and inserted into Firefly luciferase vector pGL4.22 (Promega, #E6771) at KpnI and HindIII restriction sites. 293T cells at 50%–70% confluency were transfected with 755 ng

(3) mDia formins form hetero-oligomers and cooperatively maintain murine hematopoiesisPLoS GenetDecember 29, 2023Zhaofeng Li, Meng Su, Xinshu Xie, Pan Wang, Honghao Bi, Ermin Li, Kehan Ren, Lili Dong, Zhiyi Lv, Xuezhen Ma, et al.sequence (CCCTATATGG CCTTATATGG CCATATATGG CCAAATAAGG) were synthesized and cloned into pGL4.22 (Promega, E6771) between KpnI and BglII. The oligonucleotide sequences for cloning experiments have been shown in S1

(4) Differential regulation of MYC expression by PKHD1/Pkhd1 in human and mouse kidneys: phenotypic implications for recessive polycystic kidney diseaseFrontiers in Cell and Developmental BiologyNovember 17, 2023Naoe Harafuji, Chaozhe Yang, Maoqing Wu, Girija Thiruvengadam, Heather Gordish-Dressman, R. Griffin Thompson, P. Darwin Bell, Avi Z. Rosenberg, Claudia Dafinger, Max C. Liebau, et al.ATT CTG TCC ACG G -3′ primer set. pGL4.22 [luc2CP/Puro] vector was purchased from Promega (Cat. No. E6771). pRL-TK vector was purchased from promega (Cat. No. E2241). pGL4.22-mouse Myc P1 (pGL4.22-mMyc P1):

(5) Functional analysis of CTLA4 promoter variant and its possible implication in colorectal cancer immunotherapyFront Med (Lausanne)August 3, 2023Mariana Angulo-Aguado, Sarah Orjuela-Amarillo, Julián Francisco Mora-Jácome, Lea Paloma Córdoba, Antonio Gallego-Ortiz, Cristian Camilo Gaviria-Sabogal, Nora Contreras, Carlos Figueroa, Oscar Ortega-Recalde, Adrien Morel, et al.Supplementary Table S1. The PCR products were digested and ligated into the pGL4.22luc2CP/Puro plasmid (#E6771/Promega) following the manufacturer’s instructions. The constructs were sequenced to verify the generation

(6) Eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1) expression in glioblastoma is driven by ETS1- and MYBL2-dependent transcriptional activationCell Death DiscoveryDecember 1, 2022Laura Hauffe, Daniel Picard, Julian Musa, Marc Remke, Thomas G. P. Grünewald, Barak Rotblat, Guido Reifenberger, Gabriel Leprivierinserted into the SacI and BglII restriction sites of the Firefly Luciferase expressing pGL4.22 plasmid (E6771, Promega, Madison, WI, USA). Cloning was performed by GENEWIZ Germany GmbH (Leipzig, Germany). Luciferase

(7) A regulatory variant at 3q21.1 confers an increased pleiotropic risk for hyperglycemia and altered bone mineral densityCell MetabolismMarch 2, 2021Nasa Sinnott-Armstrong, Isabel S. Sousa, Samantha Laber, Elizabeth Rendina-Ruedy, Simon E. Nitter Dankel, Teresa Ferreira, Gunnar Mellgren, David Karasik, Manuel Rivas, Jonathan Pritchard, et al.plasmid,Addgene,41815 gRNA cloning vector,Addgene,41824 pGL4.22 firefly luciferase reporter vector,Promega,E6771 pRL-Ubi,Promega,E2241 TK promoter control vector,"Hans Hauner lab, TUM",N/A doxycycline-inducible

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