pGL4.74[hRluc/TK] Vector from Promega

Product Specs
ItempGL4.74[hRluc/TK] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6921
HostMammalian
PromoterSV40
Reportersynthetic Renilla gene
Quantity20microg
Origin of ReplicationColEI-derived plasmid replication origin
Vector NamepGL4.74[hRluc/TK] Vector
Molecular Weight~53kDa

Specifications/Features

Vector Size:
4237 bp

Features:
The following features are available for the pGL4 Vectors:

• two reporter genes: a choice of firefly luciferase (luc2) or Renilla luciferase (hRluc)
• reporter genes that code for stable or destabilized proteins
• basic vectors without promoters
• vectors with promoters such as HSV-TK, SV40 and CMV
• mammalian selectable marker for hygromycin, neomycin and puromycin resistance

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Profiling the epigenomic landscape of late embryonic and adult mouse hind limb musclesScientific ReportsFebruary 17, 2026Samantha R. Queeno, Alexander S. Okamoto, Damien M. Callahan, Matthew C. O’Neill, Terence D. Capellini, Kirstin N. Sternerexpression. All reporter vector constructs were co-transfected with a pGL4.74 [hRluc/TK] Renilla vector (E6921, Promega) to control for transfection efficiency. After optimization, cells were harvested 48 h post-transfection

(2) Brain-derived estrogens facilitate male-typical behaviors by potentiating androgen receptor signaling in medakaeLifeJanuary 13, 2026Yuji Nishiike, Shizuku Maki, Daichi Miyazoe, Kiyoshi Nakasone, Yasuhiro Kamei, Takeshi Todo, Tomoko Ishikawa-Fujiwara, Kaoru Ohno, Takeshi Usami, Yoshitaka Nagahama, et al.,Thermo Fisher Scientific,cat#:K480001, Recombinant DNA reagent,pGL4.10,Promega,cat#:E6651, Recombinant DNA reagent,pGL4.74,Promega,cat#:E6921, Recombinant DNA reagent,Medaka bacterial artificial chromosome (BAC) clone containing the ara locus,NBRP

(3) A Pleiotropic and Functionally Divergent RAC3 Variant Disrupts Neurodevelopment and Impacts OrganogenesisCellsSeptember 24, 2025Ryota Sugawara, Marcello Scala, Sara Cabet, Carine Abel, Louis Januel, Gaetan Lesca, Laurent Guibaud, Frédérique Le Breton, Hiroshi Ueda, Hidenori Tabata, et al.reporter plasmids were obtained from Promega (Madison, WI, USA), including pGL4.74[hRluc/TK] (Cat# E6921, control reporter), pGL4.44[luc2P/AP1-RE/Hygro] (Cat# E4111, AP1-luciferase reporter), and pGL4.32[luc2P/NF-κB-RE/Hygro]

(4) Super‐Enhancer‐Mediated DLX5 Activation Defines Regulatory Mechanisms in Human Embryonic Stem Cell‐Derived OsteoblastsDevelopment, Growth & DifferentiationSeptember 2, 2025Shinse Fujita, Shoichiro Tani, Hiroyuki Okada, Taku Saito, Sakae Tanaka, Shinsuke Ohba, Ung‐il Chung, Hironori Hojotested regulatory elements and 9.556 fmol/well of thymidine kinase promoter–Renilla luciferase vector (E6921; Promega) were introduced by Fugene HD transfection reagent (E2311; Promega). One day after transfection,

(5) Elaidic acid drives cellular senescence and inflammation via lipid raft-mediated IL-1R signalingiScienceAugust 6, 2025Ryota Kojima, Yusuke Hirata, Ryo Ashida, Miki Takahashi, Ryosuke Matsui, Kotaro Hama, Ayako Watanabe, Ryo Takita, Emiko Sato, Taiki Abe, et al.NF-kB-RE Hygro], Cat#E8491, Promega) and thymidine kinase Renilla luciferase (pGL4.74[hRluc/TK], Cat#E6921, Promega), and incubated for 24 h. After removing the culture supernatant, 20 μL of Passive Lysis buffer

(6) Unlocking the Puzzle of Mammalian Transfection: The Role of the RNA-sensing-Mediated Interferon Response in the Cellular Defense Against Foreign DNA IntrusionInternational Journal of Biological SciencesJune 9, 2025Xiaoyu Li, Yicen Zhou, Jie Wei, Wei Sun, Ligang Fan, Jian YanpcDNA3.1(+) EGFP (Addgene, 129020), pGL4.23[luc2-minP] (Addgene, 226458), pGL4.74[hRluc/TK] (Promega, E6921), and pUC19 (Addgene, 50005). Additionally, the linear DNA constructs pcDNA3.1-neo (KpnI), CasRX pre-gRNA

(7) Widespread variation in molecular interactions and regulatory properties among transcription factor isoformsMolecular CellApril 3, 2025Luke Lambourne, Kaia Mattioli, Clarissa Santoso, Gloria Sheynkman, Sachi Inukai, Babita Kaundal, Anna Berenson, Kerstin Spirohn-Fitzgerald, Anukana Bhattacharjee, Elisabeth Rothman, et al.al.60,N/A pGL4.23[luc2/minP]-GW,Fuxman Bass et al.4,N/A pEZY3-VP160,Santoso et al124,N/A pGL4.74[hRlUc/TK],Promega,E6921 pEZY3-DB,Lu et al160,N/A Gateway pDEST15 Vector,Thermo Fisher Scientific,11802014 pt7CFE1-NHis-GST-CHA

(8) c-Ski is a novel repressor of NF-κB through interaction with p65 and HDAC1 in U937 cellsCell Commun SignalApril 2, 2025Yan Peng, Ren-Ping Xiong, Bo Wang, Xing Chen, Ya-Lie Ning, Yan Zhao, Nan Yang, Jing Zhang, Chang-Hong Li, Yuan-Guo Zhou, et al.(Cambridge, MA, UK). The pGL4.32 [luc2P/NF-kB-RE/Hygro] vector (E8491), pGL4.74 [hRluc/TK] vector (E6921), FuGENE 6 Transfection Reagent (E2693) and Dual-Glo Luciferase Assay System (E2920) were obtained from

(9) Single-Chain Nanobody Inhibition of Notch and Avidity Enhancement Utilizing the β-Pore-Forming Toxin AerolysinACS Chemical BiologyMarch 21, 2025Andrew C. D. Lemmex, Jeremy Allred, Jason Ostergard, Jake Trask, Hannah N. Bui, Michael J. M. Anderson, Benjamin Kopp, Oakley Streeter, Adam T. Smiley, Natalia S. Babilonia-Díaz, et al.1 ng/well TK::Renilla Luciferase, both of which were purchased from Promega, item numbers C9351 and E6921 respectively. The DLL4 coated plate was then washed 2×100uL PBS and transfected cells were plated on

(10) Rare variant analyses in 51,256 type 2 diabetes cases and 370,487 controls reveal the pathogenicity spectrum of monogenic diabetes genesNature GeneticsNovember 1, 2024Alicia Huerta-Chagoya, Philip Schroeder, Ravi Mandla, Jiang Li, Lowri Morris, Maheak Vora, Ahmed Alkanaq, Dorka Nagy, Lukasz Szczerbinski, Jesper G. S. Madsen, et al.construct and 0.1 µg of the internal control, Renilla-expressing pGL7.74 vector (Promega, cat. no. E6921), using 1 µl of Lipofectamine 2000 (Thermo Fisher Scientific, cat. no. 11668019) per 1 µg of construct.

(11) Functional and dynamic profiling of transcript isoforms reveals essential roles of alternative splicing in interferon responseCell GenomicsOctober 9, 2024Mahoko Takahashi Ueda, Jun Inamo, Fuyuki Miya, Mihoko Shimada, Kensuke Yamaguchi, Yuta KochiUniversity",N/A p55C1B-Luc vector,"Dr. Fujita, Kyoto University",N/A pGL4.74 vector,Promega,Cat# E6921 X-tremeGENE HP Transfection Reagent,Roche,Cat# 6366244001 Dual Luciferase Reporter assay system,Promega,Cat#N1630 ,, Deposited

(12) Variants in tubule epithelial regulatory elements mediate most heritable differences in human kidney functionNature GeneticsOctober 1, 2024Gabriel B. Loeb, Pooja Kathail, Richard W. Shuai, Ryan Chung, Reinier J. Grona, Sailaja Peddada, Volkan Sevim, Scot Federman, Karl Mader, Audrey Y. Chu, et al.were transfected with 50ng regulatory element plasmid and 50ng Renilla plasmid (pGL 4.74; Promega, E6921) using 0.3 μL Fugene HD (Promega, E2311). 24h post-transfection, regulatory element activity was measured

(13) KIF22 regulates mitosis and proliferation of chondrocyte cellsiScienceJuly 19, 2024Kawaue H, Matsubara T, Nagano K, Ikedo A, Rojasawasthien T, Yoshimura A, Nakatomi C, Imai Y, Kakuta Y, Addison WN, et al.vector pAxEFwtit2,Takara Bio,Cat#6174 pTagRFP-tubulin,Evrogen,Cat#FP145 pGL4.74[hRluc TK],Promega,Cat#E6921 pBiT1.1-C [TK/LgBiT] Vector,Promega,Cat#N196A pBiT2.1-C [TK/SmBiT] Vector,Promega,Cat#N197A pGL4.53[luc2/PGK] Vector

(14) Stage-specific GATA3 induction promotes ILC2 development after lineage commitmentNature communicationsJuly 5, 2024Furuya H, Toda Y, Iwata A, Kanai M, Kato K, Kumagai T, Kageyama T, Tanaka S, Fujimura L, Sakamoto A, et al.vectors or MSCV-Gata3-ires-Thy1.1 vectors in the presence of pGL4.74[hRluc/TK] vectors (Promega, Cat#E6921). Twenty-four hours later, RLUs were assessed with a dual luciferase assay system (Promega, Cat#E1910).

(15) Dominant negative OTULIN-related autoinflammatory syndromeJournal of Experimental MedicineJune 3, 2024Sophia Davidson, Yuri Shibata, Sophie Collard, Hongyu Zheng, Klara Kong, June M. Sun, Pawat Laohamonthonkul, Anthony Cerra, Tobias Kratina, CIRCA, et al.(pGL4.32[luc2P/NF-κB-RE/Hygro]) (#E8491; Promega) and 40 ng/well Renilla control (pGL4.74-hRluc/TK) (#E6921; Promega), as well as stated concentrations of plasmid C129S OTULIN, Y244C OTULIN, N341D OTULIN (see

(16) BRMS1L confers anticancer activity in non-small cell lung cancer by transcriptionally inducing a redox imbalance in the GPX2-ROS pathwayTranslational OncologyMarch 1, 2024Penglong Cao, Juebin Gu, Mulin Liu, Yingxin Wang, Mingying Chen, Yizhu Jiang, Xiaoyan Wang, Siqi Zhu, Xue Gao, Shijun Li48 h, luciferase activities were quantified by using a Dual-Luciferase Reporter Assay Kit (Promega, E6921, Wisconsin, USA) according to the manufacturer's instructions on an Enspire 2300 multilabel reader (Perkin

(17) RNF40 epigenetically modulates glycolysis to support the aggressiveness of basal-like breast cancerCell Death & DiseaseSeptember 28, 2023Evangelos Prokakis, Shaishavi Jansari, Angela Boshnakovska, Maria Wiese, Kathrin Kusch, Christof Kramm, Christian Dullin, Peter Rehling, Markus Glatzel, Klaus Pantel, et al.luciferase reporter (Plasmid #83467, Addgene) or the HIP-flash (#83466, Addgene) and the pGL4.74[hRluc/TK] (E6921, Promega) Renilla control reporter using the TransIT-2020 transfection reagent (Mirus) based on manufacturer’s

(18) Fluid shear stress-modulated chromatin accessibility reveals the mechano-dependency of endothelial SMAD1/5-mediated gene transcriptioniScienceSeptember 15, 2023Jerome Jatzlau, Paul-Lennard Mendez, Aybuge Altay, Lion Raaz, Yufei Zhang, Sophia Mähr, Akin Sesver, Maria Reichenbach, Stefan Mundlos, Martin Vingron, et al.DNA,Recombinant DNA ,, pGL4.17[luc2/Neo],Promega,"Cat#E6721, DQ188837" pGL4.74[hRluc/TK],Promega,"Cat#E6921, AY738230" "pGL4.17[luc2/Neo] containing SMAD1-bound regulatory regions 17,22,32 or 60k of human UNC5B",This

(19) Alpha-tocopherylquinone differentially modulates claudins to enhance intestinal epithelial tight junction barrier via AhR and Nrf2 pathwaysCell reportsJuly 25, 2023Ganapathy AS, Saha K, Wang A, Arumugam P, Dharmaprakash V, Yochum G, Koltun W, Nighot M, Perdew G, Thompson TA, et al.MA",RRID:Addgene_12260 pLminP_Luc2P_RE5,"Addgene, Watertown, MA",RRID:Addgene_90339 pGL4.74,"Promega, Madison, WI",Cat# E6921 luc2P/XRE pGL4.43,"Promega, Madison, WI",Cat #E4121 luc2P/ARE pGL4.37,"Promega, Madison, WI",Cat#

(20) A Syx-RhoA-Dia1 signaling axis regulates cell cycle progression, DNA damage, and therapy resistance in glioblastomaJCI InsightJuly 10, 2023Wan-Hsin Lin, Ryan W. Feathers, Lisa M. Cooper, Laura J. Lewis-Tuffin, Jiaxiang Chen, Jann N. Sarkaria, Panos Z. Anastasiadis(pGL4.34[luc2P/SRF-RE/Hygro], Promega E1350), and human Renilla luciferase (pGL4.74[hRluc/TK], Promega, E6921) were used in the study. The MISSION shRNAs in the pLKO.1 lentiviral vector with a puromycin resistance

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