pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) IGFL2 suppresses gastric cancer cell sensitivity to docetaxel by activating the Toll-like receptor signaling pathwayiScienceJune 19, 2026Wei Huang, Zhirui Tao, Xiaolin Xu, Huangming Cao, Li Cheng, Jin LiDNA ,, pGL3-TLR4 promoter reporter plasmid,GeneChem,N/A pRL-TK Renilla luciferase plasmid,Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism 9,GraphPad

(2) Microsomal triglyceride transfer protein restricts steroid production in Leydig cells by regulating SREBP2iScienceJune 19, 2026Atrayee Chattopadhyay, Munichandra Babu Babu Tirumalasetty, Thomas Palaia, Bhargavi Gangula, Rachel Ruoff, Qing Robert Miao, M. Mahmood HussainS1 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pSynSRE-T-Luc,Addgene,Cat #60444 pRL-TK,Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism,GraphPad

(3) Gemcitabine activates the Hippo signaling pathway and suppresses tumor growth by stabilizing large tumor suppressor kinase 2 through the hypoxia-inducible factor 1-alpha/ubiquitin protein ligase E3 component N-recognin 5 axisJ Cell Commun SignalJune 17, 2026Jin Zhang, Lixia Xu, Kai Guo, Weijia Dong, Xin Dai, Donghua Guco‐transfected with 400 ng of reporter plasmid, 50 ng of the Renilla luciferase internal control plasmid pRL‐TK (E2241, Promega, USA), and 400 ng of oe‐HIF1A or an equivalent amount of empty vector. After 24 h, cells were

(4) PRDM1 Drives a TIM3+ Macrophage Immunosuppressive Niche via LGALS9 Signaling in Prostate Cancer ProgressionOncology ResearchJune 16, 2026Yangyang Zhang, Ilyar Mamtili, Yonghao Chen, Guangjie Ji, Chaozhao LiangRenilla luciferase internal control plasmid used for normalization was the pRL-TK Vector (Promega, Cat# E2241, Madison, WI, USA). For each well of a 24-well plate, cells were co-transfected with 400 ng of HAVCR2

(5) CL-316243 facilitates stable atherosclerotic plaque phenotypes in association with suppression of perivascular adipose tissue ferroptosis via upregulating C/EBPβRedox BiolJune 13, 2026Yuanqing Jiang, Yi Li, Kefan Ma, Suxiang Guo, Nachuan Liao, Jiayi Zhou, Junbo Chen, Ruizhe Ren, Yaohui Kou, Jinying Li, et al.1 μg of GPX4 plasmid (WT or Mutant 1-5), 1 μg of empty vector or C/EBPβ and 0.2 μg pRL-TK (Promega, E2241). Cells were cultured for 48 h and detected using the Dual Luciferase Reporter Gene Assay Kit (YEASEN,

(6) Hesperetin-Black Phosphorus Nanosheets Targeting TAMs Induce Ferroptosis and Reverse Protumoral Polarization to Remodel the Immune MicroenvironmentMaterials Today BioMay 29, 2026Liang Feng, Huan Wang, Zuodi Liang, Ying Tian, Yang Guo, Zhuo Liwere co-transfected with these constructs (1 μg) and the pRL-TK Renilla reference plasmid (0.1 μg, E2241, Promega) employing Lipofectamine 3000 (Thermo Fisher Scientific, USA). After a 48-h incubation, cell

(7) CDK4 Mediates Cisplatin Resistance in Renal Cell Carcinoma (RCC) Cells by Regulating the ASH1L-CTR1 AxisOncology ResearchMay 21, 2026Wenjian Zeng, Xianglong Li, Hao Cai, Qingyu Zhou, Shuangshuang Sun, Pingping Li, Sunlong Li, Zhi Chenof the internal reference vector pRL-TK (containing the Renilla luciferase gene, Promega, Cat. No.: E2241, Madison, USA) to correct transfection efficiency, 2 μL of Lipofectamine 3000 transfection reagent (Thermo

(8) Equine infectious anemia virus blocks interferon responses through Rev-mediated activation of the stress granule-PKR-eIF2α pathwayPLoS PathogMay 21, 2026Huiling Ren, Kewei Chen, Bingqian Zhou, Weiguo Zhang, Xue-Feng Wang, Xiaojun Wang(Sigma-Aldrich, E7658). The pEGFP-N1 vector (HG-VYC0086) was purchased from Clontech. The pRL-TK vector (E2241) was purchased from Promega. The mutants of Rev and G3BP1 were generated using PCR-based site-directed mutagenesis

(9) Nuclear factor IX promotes endometriosis progression through transcriptional activation of tetraspanin-2J Mol Med (Berl)May 16, 2026Yuqi Liu, Qing Xue, Jingwen Zhu, Cheng Zeng, Xin Li, Jing Shang, Jie Li, Peili Wuluciferase reporter construct, along with 90 ng of Renilla luciferase reporter plasmid pRL-TK (Promega, E2241) as an internal control. Cells were gathered 48 h post transfection, and the luciferase assay system

(10) Prenatal bisphenol A exposure reprograms SF1-lactylation pathways to promote endometriosis susceptibilityiScienceMay 15, 2026Xiaohan Liu, Yanxia Fu, Liuxuan Huang, Donghan Li, Paul Yao, Liqin Zengluciferase reporter vector,Promega,Cat# E1751 Renilla luciferase control vector (pRL-TK),Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, ImageJ software,National

(11) HDAC7-Induced Epigenetic Repression Modulates ATF3 Functional Plasticity in Colorectal Cancer PathogenesisInt J Biol SciMay 1, 2026Qi Wang, Donglei Ji, Yanjie Jia, Shuang Li, Wenjing Gao, Tingting Liang, Yingying Liang, Caroline Zeng, Chunyu Wang, Ka Lung Cheung, et al.(-1486 to +114 relative to TSS), was co-transfected with the pRL-TK normalization vector (Promega, E2241) in a 10:1 ratio using Lipofectamine 3000. Firefly and Renilla luciferase activities were measured sequentially

(12) Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cellsTranslational Cancer ResearchApril 30, 2026Huaibin Zhang, Rongxuan Liu, Xiaorong Liu, Wenqiang Liang, Wenji Wang, Yongping WangLipo6000TM transfection reagent. A Renilla luciferase plasmid (pRL-TK, 0.02 µg/well, Promega, USA, Cat. No. E2241) was co-transfected as an internal control. Forty-eight hours post-transfection, the luciferase activity

(13) Tissue-selective COPII modulator SEC16B aggravates cardiovascular disease by promoting lipid exportThe EMBO JournalApril 24, 2026Xiao Wang, Yating Hu, Lu Liu, Runze Huang, Yawei Wang, Bing Liu, Yifei Zhao, Yuangang Zhu, Jia Lv, Liang Liu, et al.2018 10.1073/pnas.1704639115" Firefly,luciferase Promega,Cat# E1751 Renilla,luciferase Promega,Cat# E2241 AAV- HCRApoE/hAAT-hPCSK9 D374Y,Addgene,Cat #58379 CMV-HA-SEC13,This paper,N/A CMV-mGFP-SEC61β,This

(14) MYC-bound enhancer RNAs in cis regulate gene transcription and tumorigenesisSci AdvApril 24, 2026Sihan Li, Zehua Wang, Phuong Nguyen, Yue Wang, Yueshan Zhao, Yifei Wang, Yuang Chen, Haozhe Huang, Xiaofei Wang, Yu Zhang, et al.transfected into MCF-7 cells using Lipofectamine 2000, and plasmid pRL-TK expressing Renilla (Promega, E2241) was used as an internal control reporter. After transfection for 24 hours, cells were subjected to

(15) Integrated drivers of basal and acute immunity in diverse human populationsCell Reports MedicineApril 21, 2026Aisha Hegab Souquette, E. Kaitlynn Allen, Christine M. Oshansky, Evadnie Rampersaud, Emily V. Walker, Li Tang, Sook-San Wong, Trushar Jeevan, Lei Shi, Stanley Pounds, et al.CO2. The following day, 1μg of pGL4.10 test plasmid and 0.01μg of pRL-TK control plasmid (Promega Cat. E2241) were added per well, according to the TransIT LT protocol. All transfections were conducted in triplicate.

(16) Extracellular signalling regulates gastrin transcription through site-specific phosphorylation and nuclear redistribution of MeninbioRxivApril 13, 2026Uloma B. Elvis-Offiah, Ziyu Wen, Xianxin Hua, Juanita L. Merchanttransfection efficiency, cells were also co-transfected with the PRL-TK Renilla Reporter Plasmid (Promega, #E2241). After 24 h, cells were serum-starved for 24 h, followed by treatment with either recombinant human

(17) Microbiota-derived secondary bile acids promote STING activation and antitumor activitybioRxivApril 12, 2026Xinglin Yang, Xing Zhang, Weichao Li, Anant Gharpure, Althea Hansel-Harris, Andreas F. Tillack, Chongyang Wu, Yan-Zhu Hsieh, Ariana Sulpizio, Kyong T. Fam, et al.including pMSCV-hygro-STING (Addgene, 102598), IFN-Beta_pGL3 (Addgene, 102597), and pRL-TK (Promega, E2241) using Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000–015). After 24 h, cells were treated

(18) Secretory VSMC-derived THBS1 promotes macrophage M1 polarization and inflammation in intracranial aneurysms via the CD47/NF-κB axisFront ImmunolApril 10, 2026Jianhuang Huang, Qixiu Wang, Yangyang Lin, Jianning Chen, Caihou Linluciferase reporter plasmid (Clontech, 631744) and 50 ng/well pRL-TK Renilla luciferase plasmid (Promega, E2241) as an internal control. After 48 h of co-culture, cells were lysed and luciferase activities were measured

(19) NR3C1 Modulates Wnt Signalling to Influence the Invasiveness and Immune Features of Nonfunctioning Invasive Pituitary AdenomasJournal of Cellular and Molecular MedicineApril 1, 2026Xiaoping Wang, Jinfeng Zhang, Tao Jiang, Zhijun Yang, Yu Zhang, Pinan Liu, Yuanxiang Lin#12456) or FOPflash (Addgene, #12457) (500 ng each) and the Renilla internal control vector (Promega, E2241, pRL‐TK, 50 ng), along with sh‐NC or sh‐NR3C1 as required. Cells were lysed 48 h post‐transfection,

(20) ZXDB Drives Macrophage Inflammatory Programming in Sepsis-Induced Acute Kidney Injury by Recruiting EIF4A3 to Enhance ACACA TranslationFASEB JMarch 31, 2026Haiyang Wu, Wei Gang, Li Wang, Jing Sun, Shuangxi Li, Zhiyong Guoco‐transfected with the ACACA 5′UTR reporter construct, a control Renilla luciferase plasmid (pRL‐TK, E2241; Promega), and corresponding expression plasmids (ZXDB OE, EIF4A3 KD). Firefly luciferase activity was

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pRL-TK Vector

pRL-TK Vector from Promega

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Promega

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pRL-TK Vector from Promega

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