pGL4.51[luc2/CMV/Neo] Vector from Promega

Product Specs
ItempGL4.51[luc2/CMV/Neo] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1320
HostMammalian
Selectable MarkerHygromycin
ResistanceAmpicillin
Quantity20microg
Origin of ReplicationColE1-derived plasmid origin of replication
PromoterMinimal promoter
Vector NamepGL4.51

Specifications/Features

Vector Size:
6358 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) SIRT5-modified human umbilical cord mesenchymal stem cells loaded with antioxidant polydopamine nanozyme enhance parpi resistance in ovarian cancer via fatty acid metabolism reprogrammingJ NanobiotechnologyJuly 4, 2025Jin Zhang, Xiuluan Du, Xin Dai, Yanxiang Liu, Kai Guo, Donghua Gucell line was transfected with an expression vector containing the firefly luciferase gene (luc2) (E1320, Promega, USA). Stable luciferase-expressing ovarian cancer cell lines were established through G418

(2) Immune cell infiltration into brain tumor microenvironment is mediated by Rab27-regulated vascular wall integritySci AdvMay 23, 2025Lata Adnani, Brian Meehan, Minjun Kim, Dongsic Choi, Christopher E. Rudd, Yasser Riazalhosseini, Janusz RakGateway cassette and SFFV promoter (83, 84) with luciferase gene cloned from pGL4.51(luc2/CMV/Neo) (E1320 Promega) (obtained from K. Eppert, McGill University). GL261 cells were transduced with BFP-OVA lentiviral

(3) Biologically targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett’s esophagus and esophageal adenocarcinomaHuman Genetics and Genomics AdvancesApril 1, 2025Li Yan, Qianchuan He, Shiv P. Verma, Xu Zhang, Ann-Sophie Giel, Carlo Maj, Kathryn Graz, Elnaz Naderi, Jianhong Chen, Mourad Wagdy Ali, et al.Sanger sequencing. The constitutively active Firefly luciferase vector pGL4.51 (luc2/CMV/Neo) (Promega, no. E1320) was used for normalization in luciferase assays. Cell culture and transfection OE19 and OE33 EAC

(4) Case-Case Genome-Wide Analyses Identify Subtype-Informative Variants That Confer Risk for Breast CancerCancer ResearchAugust 1, 2024Xiaohui Sun, Shiv Prakash Verma, Guochong Jia, Xinjun Wang, Jie Ping, Xingyi Guo, Xiao-Ou Shu, Jianhong Chen, Andriy Derkach, Qiuyin Cai, et al.Sanger sequencing. The constitutively active Firefly-luciferase vector pGL4.51 (luc2/CMV/Neo) (Promega #E1320) was used for normalization in luciferase assays. Cell culture and transfection The MCF-7 cell line

(5) Case-Case Genome-Wide Analyses Identify Subtype-Informative Variants that Confer Risk for Breast CancerCancer ResAugust 1, 2024Xiaohui Sun, Shiv Prakash Verma, Guochong Jia, Xinjun Wang, Jie Ping, Xingyi Guo, Xiao-Ou Shu, Jianhong Chen, Andriy Derkach, Qiuyin Cai, et al.Sanger sequencing. The constitutively active Firefly-luciferase vector pGL4.51 (luc2/CMV/Neo) (Promega #E1320) was used for normalization in luciferase assays. Cell culture and transfection The MCF-7 cell line

(6) Mesenchymal glioma stem cells trigger vasectasia—distinct neovascularization process stimulated by extracellular vesicles carrying EGFRNature CommunicationsApril 3, 2024Cristiana Spinelli, Lata Adnani, Brian Meehan, Laura Montermini, Sidong Huang, Minjun Kim, Tamiko Nishimura, Sidney E. Croul, Ichiro Nakano, Yasser Riazalhosseini, et al.promoter (PMID: 15619618 and PMID: 24776803) and with luciferase gene cloned from pGL4.51(luc2/CMV/Neo) (E1320 Promega) (kindly obtained from Dr. K. Eppert, McGill University). Finally, the pellets of viral particles

(7) FOXO1 promotes cancer cell growth through MDM2-mediated p53 degradationJournal of Biological ChemistryApril 1, 2024Haruki Tomiyasu, Makoto Habara, Shunsuke Hanaki, Yuki Sato, Yosei Miki, Midori Shimadawith 48 ng pNL[NlucP/p53-RE/Hygro] Vector (CS194102; Promega) and 2 ng pGL4.51[luc2/CMV/Neo] Vector (E1320; Promega) using 150 ng PEI Max (24765-1; Polyscience). Forty-eight hours after transfection, NanoLuc

(8) N6-methyladenosine in 5′ UTR does not promote translation initiationMolecular CellFebruary 1, 2024Ewelina Guca, Rodrigo Alarcon, Michael Z. Palo, Leonardo Santos, Santiago Alonso-Gil, Marcos Davyt, Leonardo H.F. França di Lima, Fanny Boissier, Sarada Das, Bojan Zagrovic, et al.DNA,Recombinant DNA,Recombinant DNA ,, Plasmid pUC,Addgene,Cat #50005 Plasmid pGL4.51,Promega,Cat # E1320 Plasmid pUC 5’UTR-less,This study,N/A Plasmid pUC A(−3)-Luc with different 5’UTRs,This study,N/A ,, Software

(9) Biological and clinical significance of the AGE-RAGE axis in the aggressiveness and prognosis of prostate cancerJournal of food and drug analysisDecember 15, 2023Khoo SH, Wu PR, Yeh KT, Hsu SL, Wu CH.expressed firefly luciferase was generated by transfecting DU145 cells with pGL4.51[luc2/CMV/Neo] vectors (E1320, Promega). Xenograft experiments were performed with 5-week-old NOD/ SCID mice. The animals were randomized

(10) Calcineurin-mediated dephosphorylation enhances the stability and transactivation of c-MycScientific reportsAugust 12, 2023Masaki T, Habara M, Hanaki S, Sato Y, Tomiyasu H, Miki Y, Shimada M.with 48 ng pNL[NlucP/MycMax-RE/Hygro] Vector (Promega) and 2 ng pGL4.51[luc2/CMV/Neo] Vector (Promega, E1320) using 150 ng Polyethylenimine Max (24765-1, Polyscience). Forty-eight hours after transfection, NanoLuc

(11) Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challengeBiomaterialsJanuary 1, 2023Sun S, Li E, Zhao G, Tang J, Zuo Q, Cai L, Xu C, Sui C, Ou Y, Liu C, et al.was purchased from Invitrogen (11668019, Carlsbad, USA). pGL4.51-Luciferase Reporter Vectors (pFLuc, E1320) was purchased from Promega (Madison, USA). Plasmids encoding SARS-CoV-2 S protein (pSpike) and pVax

(12) Tumor-suppressive MEG3 induces microRNA-493-5p expression to reduce arabinocytosine chemoresistance of acute myeloid leukemia cells by downregulating the METTL3/MYC axisJournal of Translational MedicineDecember 1, 2022Airong Wang, Yufei Chen, Luyao Shi, Mengya Li, Lingling Li, Shujuan Wang, Chong Wangwere constructed. Firefly luciferase sequence after codon optimization was separated from pGL4.51 (E1320; Promega, Madison, WI) and cloned into the lentiviral vector pCDH-CMV-MCS-EFS-copGFP to produce pCDH-luc-CopGFP

(13) Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimicsEMBO molecular medicineDecember 7, 2021Olmeda D, Cerezo-Wallis D, Mucientes C, Calvo TG, Cañón E, Alonso-Curbelo D, Ibarz N, Muñoz J, Rodriguez-Peralto JL, Ortiz-Romero P, et al.plasmids were transfected into SK‐Mel‐147 cells or HLEC. Cells were co‐transduced with pGL4.52‐Luc2 (#E1320; Promega) vector as transfection control. Luciferase activity was monitored 16 h thereafter (in the

(14) Chemogenetic ON and OFF switches for RNA virus replicationNature CommunicationsDecember 1, 2021E. Heilmann et al.luciferase cassette, amplified via PCR (primer pair XhoI-luc-for/NheI-luc-rev) from pGL4.51 (Promega #E1320) via XhoI/NheI restriction sites. VSV-Pprot-luciferase was cloned analogously to the VSV-Pprot-GFP variant

(15) GL261 luciferase-expressing cells elicit an anti-tumor immune response: an evaluation of murine glioma modelsScientific reportsJuly 3, 2020Sanchez VE, Lynes JP, Walbridge S, Wang X, Edwards NA, Nwankwo AK, Sur HP, Dominah GA, Obungu A, Adamstein N, et al.with 10% FBS and passaged (1:2) every 3 days. GL261 cells were transfected with DNA plasmid pGL4.51 (E1320), encoding luciferase reporter luc2 using Promega FuGENE 6 transfection reagent. After establishment

(16) Development of an Orthotopic Intrasplenic Xenograft Mouse Model of Canine Histiocytic Sarcoma and Its Use in Evaluating the Efficacy of Treatment with DasatinibComparative MedicineFebruary 6, 2019Marilia Takada, Lauren A Smyth, Jeremy ML Hix, Sarah M Corner, Sandrao'reilly, Matti Kiupel, Vilma Yuzbasiyan-Gurkanbioluminescence imaging (BLI). Briefly, cells were incubated with vector pGL4.51 (luc2/CMV/Neo; catalog no. E1320, Promega, Madison, WI) and Lipofectamine 2000 reagent (catalog no. 11668027, ThermoFisher) for a maximum

(17) Integrated Molecular Profiling of Human Gastric Cancer Identifies DDR2 as a Potential Regulator of Peritoneal DisseminationScientific ReportsApril 1, 2016Junji Kurashige et al.penicillin, and 100 mg/mL streptomycin. 58As9 cells were transfected with the pGL4.51 [luc2/CMV/Neo] Vector (E1320 Cloning Systems, Promega, Madison, WI) according to the manufacturer’s instructions. Stable transfectants

(18) Enhancement of the in vivo persistence and antitumor efficacy of CD19 chimeric antigen receptor T cells through the delivery of modified TERT mRNACell DiscoveryDecember 29, 2015Yun Bai, Shifeng Kan, Shixin Zhou, Yuting Wang, Jun Xu, John P Cooke, Jinhua Wen, Hongkui Dengby centrifugation and re-suspension. A total of 2 μg of the pGL4.51 plasmid (luc2/CMV/Neo; Promega E1320, Madison, MI, USA) per 2×106 cells was electrotransfected using the T-021 program with Amaxa Nucleofector

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