Tween 20, Molecular Biology Grade from Promega

Product Specs
ItemTween 20, Molecular Biology Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberH5151
Quantity500ml
Molecular Weight~39kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Genetic interaction between Adgrg6 and Sox9 reveals a feedforward mechanism for postnatal spinal stabilitybioRxivApril 8, 2026Valeria Aceves, Ziyi Xu, Cheng-Hai Zhang, Jongkil Kim, Joshua Ito, Kylie Mathers, Andrew B. Lassar, Zhaoyang Liu, Ryan S. Gray(10X),VWR,E442 Tricaine-methanesulfonate,Syndel,200-266 Tris-HCl,Sigma,T3253 TRIzol Reagent,Ambion by Life Technologies,15596026 Tween20,Promega,H5151 Isoflurane,Piramal Critical Care,NDC 66794-013-25 DAB Chromogenic detection kit,Vector,SK-4105 Vectastain

(2) Venezuelan Equine Encephalitis Virus Antagonizes the cGAS-STING PathwayCellsFebruary 10, 2026Brittany N. Heath, Maryna Akhrymuk, Abdullahi T. Jamiu, Ivan Akhrymuk, Alicia M. Pickrell, Kylene Kehn-HallLouis, MO, USA), 0.1% SDS (Sigma-Aldrich, L3771, Saint Louis, MO, USA), and 0.01% Tween 20 (Promega, H5151, Madison, WI, USA), pH adjusted to 2.2 with Hydrochloric acid (Avantor, JT5618, Radnor, PA, USA), in

(3) Microscopy Nodes: versatile 3D microscopy visualization with BlenderEMBO ReportsJanuary 5, 2026Aafke Gros, Chandni Bhickta, Granita Lokaj, Brady Johnston, Yannick Schwab, Simone Köhler, Niccolò BanterleGmbH,1.06404.1000 Poly-D-lysine,Merck,A-003-E BSA,Cell signaling,9998S Triton X-100,Sigma Aldrich,X100-1L Tween-20,Promega,H5151 Hoechst nuclear dye,Thermo,H1399 1-Hexadecene,Sigma Aldrich,822064 Glass-distilled acetone,EMS,10015 Uranyl

(4) ZO-1/Tjp1 and ZO-2/Tjp2 deletion in retinal pigment epithelium causes progressive retinal degenerationiScienceNovember 21, 2025Safiah Mohamed Ali, Bhav Harshad Parikh, Queenie Shu Woon Tan, Barbara Hübner, Aleksandra N. Kozyrina, Animesh Banerjee, Jie Zhao, Debbie Goh, Hanumakumar Bogireddi, Sia Wey Yeo, et al.SIN13314P TritonX-100,Promega,Cat# H5141 Tropicamide,Alcon,Cat# NDC 0998-0355-15 Tween-20,Promega,Cat# H5151 Uranyl Acetate,Electron Microscopy Sciences,Cat# 22400 Uranyl Acetate,Electron Microscopy Sciences,Cat#

(5) JAK2/STAT3 Signaling Pathway Modulates Acute Methylmercury Toxicity in the Mouse Astrocyte C8-D1A Cell LineNeurochem ResAugust 13, 2025Aafia Ahmed, Maximus Wong, Abel Santamaria, João Batista Rocha, Aaron B Bowman, Michael Aschner, Beatriz Ferrerbovine serum albumin (BSA, Fisher Scientific, BP1600) in Tris buffer saline (Bio-Rad, #1706435)- 0.1% tween-20 (Promega, H5151) (TBS-T) at room temperature to block non-specific binding, followed by incubation with primary antibodies

(6) Combined Manganese-Iron Exposure Reduced Oxidative Stress is Associated with the NRF2/NQO1 Pathway in Astrocytic C8-D1A CellsBiological Trace Element ResearchJune 17, 2025Maximus Wong, Aafia Ahmed, Wenjing Luo, Aaron B. Bowman, Yousef Tizabi, Michael Aschner, Beatriz FerrerBioReagents, USA, BP1600-100) in Tris buffer saline containing 0.1% Tween-20 (TBS-T, Promega, USA, H5151) at room temperature. Membranes were then incubated overnight at 4 °C with primary antibodies against

(7) Enhanced differentiation of neural progenitor cells in Alzheimer’s disease into vulnerable immature neuronsiScienceMay 16, 2025Joonho Cho, Simsung Bae, Juyeong Jeon, Janis Transfeld, Changyeob Lee, Alexi Nott, Fan Gao, Jinsoo SeoTriton™ X-100,Sigma-Aldrich,Cat# T9284 BSA assay kit,Bio-Rad,Cat# 5000006 "Tween® 20, Molecular Biology Grade",Promega,Cat# H5151 Fisher Healthcare Tissue-Plus™ O.C.T. Compound,Thermo Fisher Scientific,Cat# 23-730-571 Skim Milk

(8) Cell expansion for notochord mechanics and endochondral bone lengthening in zebrafish depends on the 5′-inositol phosphatase Inppl1aCurrent BiologyMay 5, 2025Brittney Voigt, Katherine Frazier, Donya Yazdi, Jace Klein, Paul Gontarz, Bo Zhang, Diane S. Sepich, Julia Mo, Joanna Smeeton, Lilianna Solnica-Krezel, et al.(10X),VWR,E442 Tricaine-methanesulfonate,Syndel,200–266 Tris-HCl,Sigma,T3253 TRIzol Reagent,Ambion by Life Technologies,15596026 Tween20,Promega,H5151 Critical commercial assays,Critical commercial assays,Critical commercial assays BioSpin P-30 Columns,BioRad,7326231 Direct-ZOL

(9) A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung diseaseThe EMBO JournalJanuary 15, 2025Kyungtae Lim, Eimear N Rutherford, Livia Delpiano, Peng He, Weimin Lin, Dawei Sun, Dick J H Van den Boomen, James R Edgar, Jae Hak Bang, Alexander Predeus, et al.(TDE),Merck,,166782 Lysotracker,Cell Signaling Technologies,,8783S Poly-D-Lysine,Biotechne,,3439-100 -01 PBS-Tween-20,Promega,,H5151 VECTASHIELD® PLUS Antifade Mounting Medium,Vector laboratories,,H-1900 SYBR Green JumpStart Taq Readymix,Sigma-Aldrich,,S4438 SYBR Green PCR Master Mix

(10) Improved protocol for histological and histopathological preparation of large eyesMicroscopy Research and TechniqueJanuary 1, 2025Mingliang Zhang, Raquel Lima e Silva, Lingli Zhou, Sean F. Hackett, Peter A. Campochiaro, Jikui Shen210‐PS‐1GFP, RRID:AB_10013682) at 23°C for 2 h. After washing with PBS containing 0.05% Tween‐20 (PBS‐T, Cat#:H5151, Tween‐20, Molecular Biology Grade, Promega, Maddison, WI), slides were counterstained with Hoechst

(11) Phospho-regulation of ASCL1-mediated chromatin opening during cellular reprogrammingDevelopmentDecember 15, 2024Roberta Azzarelli, Sarah Gillen, Frances Connor, Jethro Lundie-Brown, Francesca Puletti, Rosalind Drummond, Ana Raffaelli, Anna Philpott1620115). Membranes were blocked in 5% milk (Serva 42590.02) in TBST [TBS containing 0.1% Tween (Promega, H5151)] and then incubated overnight at 4°C with primary antibodies: rabbit anti-ASCL1 (1:1000; Abcam, ab211327-

(12) Long-term 3D cell culture models for hepatitis B virus studiesVirologyDecember 1, 2024Christopher E. Jones, Georgios Dangas, Adriana C. Norris, Madeleine Koenig, Dar-Yin Li, Taylor M. Shue, Antonis Athanasiadis, Luana Barbosa, Yichen Zhou, Kenneth C. Levenson, et al.standard stock (catalogue no. A9511; Sigma-Aldrich) with 1x DPBS with 0.05% Tween20 (catalogue no. H5151; Promega). Samples were initially diluted in a 1:200 in ELISA wash buffer (DPBS + 0.05% Tween20). For

(13) Myrtus communis L. Essential Oil Exhibits Antiviral Activity against CoronavirusesPharmaceuticals (Basel)September 10, 2024Dar-Yin Li, Matthew G. Donadu, Taylor Shue, Georgios Dangas, Antonis Athanasiadis, Shuiyun Lan, Xin Wen, Basem Battah, Stefania Zanetti, Vittorio Mazzarello, et al.and clone 541-8F (#MAB9012; Sigma-Aldrich, St. Louis, MO, USA). Cells were washed with 0.1% Tween-20 (H5151, Promega, Madison, WI, USA) in PBS, and AlexaFluor 488 Goat anti-mouse IgG (H+L) Cross-Absorbed Secondary

(14) Focused Screening Identifies Different Sensitivities of Human TET Oxygenases to the Oncometabolite 2-HydroxyglutarateJournal of Medicinal ChemistryMarch 28, 2024Roman Belle, Hilal Saraç, Eidarus Salah, Bhaskar Bhushan, Aleksandra Szykowska, Grace Roper, Anthony Tumber, Skirmantas Kriaucionis, Nicola Burgess-Brown, Christopher J. Schofield, et al.PerkinElmer, Stabilizer, 7.5% (DTPA-purified BSA), Cat: CR84-100), Tween 20 (Tween 20, Promega, ref#: H5151), disodium 2-oxoglutarate (Sigma, Cat#: K3752–100G), (+)-sodium L-ascorbate (Sigma, Cat#: 11140-50G),

(15) Hybrid structural modeling of alloantibody binding to human leukocyte antigen with rapid and reproducible cross-linking mass spectrometryCell Reports MethodsSeptember 1, 2023Zheng Ser, Yue Gu, Jiawei Yap, Yan Ting Lim, Shi Mei Wang, Maryam Hamidinia, Tanusya Murali Murali, Ragini Kumar, Nicholas RJ. Gascoigne, Paul A. MacAry, et al.Healthcare/Cytiva,Cat# 17061802 MWCO protein concentrator,Pierce,Cat# 88536 Tween 20,Promega,Cat# H5151 Skim milk powder,Merck,Cat# 70166-500G NuPage LDS Sample buffer (4x),Invitrogen,Cat# 2399420 MES

(16) Anti-angiogenic collagen IV-derived peptide target engagement with α(v)β(3) and α(5)β(1) in ocular neovascularization modelsiScienceFebruary 17, 2023Lima E Silva R, Mirando AC, Tzeng SY, Green JJ, Popel AS, Pandey NB, Campochiaro PA.56036 BSA,Cell Signaling Technologies,Cat#: 9998 "Tween 20, Molecular Biology Grade",Promega,Cat#: H5151 Super-Signal West Pico PLUS,Thermo Fisher Scientific,Cat # PI34580 Normal goat serum,Vector,Cat #

(17) Generation and timing of graded responses to morphogen gradientsDevelopmentDecember 15, 2021Shari Carmon, Felix Jonas, Naama Barkai, Eyal D. Schejter, Ben-Zion ShiloSSC+10 μg/ml ssDNA and gradually transferred to PBS-0.5% Tween 20, Molecular Biology Grade (Promega, H5151) and mounted with Vectashield+DAPI Mounting Medium Vectashield+DAPI Mounting Medium (Vector Laboratories,

(18) Microglia facilitate repair of demyelinated lesions via post-squalene sterol synthesisNature neuroscienceJanuary 1, 2021Berghoff SA, Spieth L, Sun T, Hosang L, Schlaphoff L, Depp C, Düking T, Winchenbach J, Neuber J, Ewers D, et al.System, ThermoFisher Scientific). Membranes were incubated in 1x TBS containing 0.05% Tween-20 (Promega, H5151, 1x TBS-T) and 5% w/v skim milk powder (blocking buffer) for 60 min under constant shaking at RT. Primary

(19) The novel zebrafish model pretzel demonstrates a central role for SH3PXD2B in defective collagen remodelling and fibrosis in Frank-Ter Haar syndromeBiology OpenDecember 15, 2020Ivo J. H. M. de Vos, Arnette Shi Wei Wong, Jason Taslim, Sheena Li Ming Ong, Nicole C. Syder, Julian L. Goggi, Thomas J. Carney, Maurice A. M. van Steensel[10 mM Tris (1st BASE, BIO-1400-500 g), 50 mM KCl (Merck, 102525M), 0.3% (v/v) Tween® 20 (Promega, H5151), 0.3% (v/v) Nonidet P40 substitute (United States Biological, Salem, MA, USA; N3500) and 0.488 mg/ml

(20) CD49a Regulates Cutaneous Resident Memory CD8⁺ T Cell Persistence and ResponseCell reportsSeptember 1, 2020Bromley SK, Akbaba H, Mani V, Mora-Buch R, Chasse AY, Sama A, Luster AD.Aldrich,Cat# C6158 OCT compound,Tissue-Tek,Cat# 4583 Tween20 molecular biology grade,Promega,Cat# H5151 "Hoescht 33342, Trihydrochloride, Trihydrate",Thermo Fisher Scientific,Cat# H1399 Alexa Fluor 647 Streptavidin

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