Tween 20, Molecular Biology Grade from Promega

Product Specs
ItemTween 20, Molecular Biology Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberH5152
Quantity100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Human induced pluripotent stem cell-derived inner ear organoids reveal hair cell damage and plasticity after cisplatin and gentamicin exposureDis Model MechJune 1, 2026Amy W. A. Lucassen, Winnie M. C. van den Boogaard, Esther Fousert, Jingyuan Zhang, Karl R. Koehler, John C. M. J. de Groot, Peter Paul G. van Benthem, Wouter H. van der Valk, Heiko LocherSigma-Aldrich) for 12 min at 97°C. Then, sections were rinsed with washing buffer consisting of 0.05% Tween-20 (H5152, Promega) in PBS and subsequently blocked for 30 min with blocking buffer consisting of PBS with 0.05%

(2) Protocol for spatial sampling and monitoring infection disease dynamics and pathogen spread in social ambrosia beetle coloniesSTAR ProtocolsMarch 20, 2026Abolfazl Masoudi, Ross A. Joseph, Nemat O. Keyhani1880 Potato Dextrose Agar (PDA),"Criterion™, Hardy Diagnostics, USA",Cat# 6621 Tween 20,Promega,Cat# H5152 Crystal violet,Sigma-Aldrich,Cat# C0775 CTAB,Sigma-Aldrich,Cat# H6269 Tetracycline,Sigma-Aldrich,Cat#

(3) Time-Lapse Into Immunofluorescence Imaging Using a Gridded DishBIO-PROTOCOLFebruary 20, 2026Nick Lang, Catherine G. Chu, Andrew D. StephensScience, catalog number: 9002-93-01) 8. Tween 20 molecular biology grade (Promega, catalog number: H5152) 9. 0.25% Trypsin, 0.1% EDTA in HBSS, no calcium, magnesium, and sodium bicarbonate (Corning, catalog

(4) N-(2-Hydroxyphenyl)-2-propylpentanamide Modulates HDAC1 and GPER1 Expression in a Rodent Model of Triple-Negative Breast CancerBiomedicinesJanuary 30, 2026Cynthia Ramírez-Farías, Javier Ventura-Juárez, Argelia Calvillo-Robledo, Manuel Enrique Ávila-Blanco, Daniel González-Blas, José Correa-Basurto, Andrés Quintanar Stephanowashed three times (10 min each) with TBS containing 0.05% Tween-20 (Promega, Madison, WI, USA; cat. H5152), and subsequently incubated for 2 h at room temperature with HRP-conjugated anti-rabbit secondary antibody

(5) Imaging the Intestinal Transcriptome With Multiplexed Error‐Robust Fluorescence In Situ Hybridization (MERFISH)Current ProtocolsDecember 27, 2025Paolo Cadinu, Evan Yang, Rosalind J. Xu, Brianna R. Watson, Josh Luce, Jeffrey R. Moffitt15568025) 8 M Guanidine-HCl (ThermoFisher, 24115) 0.5 M EDTA (ThermoFisher, AM9260G) Tween 20 (Promega, H5152) Proteinase K (New England Biolabs, P8107S) 20x Saline Sodium Citrate [SSC] (Invitrogen, AM9763) Nuclease-free

(6) Xist upstream deletion leads to dysregulation of Xist and autosomal gene expressionGenome ResearchSeptember 2, 2025Sudeshna Majumdar, Lakshmi Sowjanya Bammidi, Hemant C. Naik, Avinchal Manhas, Runumi Baro, Akash Kalita, Amlan Jyoti Naskar, Sundarraj Nidharshan, Girija S. Bariha, Dimple Notani, et al.#B9001S), 80 units of RNAse-OUT RNAse inhibitor (Invitrogen, #10777-019), 0.2% Tween-20 (Promega, #H5152), and PBS for 30 min in a chamber humidified using PBS/0.2% Tween-20 at 37°C. Cell samples were then

(7) Protocol optimization improves the performance of multiplexed RNA imagingScientific ReportsAugust 31, 2025Josh J. Luce, Christian A. Reardon-Lochbaum, Paolo Cadinu, Rosalind J. Xu, Jose Aceves-Salvador, Evangelia Semizoglou, Bertrand Wong, Iris Lopez, Alan B. Cantor, William Renthal, et al.solution containing 0.8 M guanidine (Thermo, 24115), 0.5% (v/v) Triton-X, 5% (v/v) Tween-20 (Promega, H5152), 1% (v/v) proteinase K (NEB, P8107S), 30 mM EDTA (Thermo, AM9262), and 50 mM Tris-HCl for 12 h at 37

(8) The NuRD component CHD3 promotes BMP signalling during cranial neural crest cell specificationEMBO ReportsAugust 20, 2025Zoe H Mitchell, Joery den Hoed, Willemijn Claassen, Martina Demurtas, Laura Deelen, Philippe M Campeau, Karen Liu, Simon E Fisher, Marco Trizzinoalso set up for each different secondary antibody. Samples were then washed in 0.1% Tween 20 (Promega, H5152) in PBS before being stained with the relevant secondary antibodies diluted in PBS for 1 h at 37 °C.

(9) High-Affinity Probes for Androgen Receptor Imaging: From Cells and In Silico Modeling to Whole-Body Fluorescent ApplicationsJ Med ChemAugust 14, 2025Saheed A. Ayodeji, Yaroslav Balytskyi, Destina Unaegbu, Allison Ingman, Christopher V. Kelly, Sheryl Robertsmethanol, blocked in 5% w/v nonfat milk (Dot Scientific, #DSM17200) in 0.05% TBS – Tween 20 (Promega, #H5152) for 1 h, cut just below the 70 kDa ladder mark, and incubated the top and bottom half of the membrane

(10) Spatial organization within social ambrosia beetle nests limits spread of infectious diseaseiScienceAugust 5, 2025Abolfazl Masoudi, Ross A. Joseph, Nemat O. Keyhani1880 Potato Dextrose Agar (PDA),"Criterion™, Hardy Diagnostics, USA",Cat# 6621 Tween 20,Promega,Cat# H5152 Crystal violet,Sigma-Aldrich,Cat# C0775 CTAB,Sigma-Aldrich,Cat# H6269 Tetracycline,Sigma-Aldrich,Cat#

(11) Multiplexed, universal probe-based rare variant detection with USE-PCRSci RepJuly 4, 2025John Alvarado, Lucien Jacky, Dominic Yurk, Aaron Aguiar, Paul Belitz, Jerrod J. Schwartzprepared with a final concentration of 1 μM unquenched ROX probe (poly(T)10) and 1% Tween-20 (Promega REF H5152). To create a set of SNV control templates, a unique plasmid was ordered for each of the selected 32

(12) Assessment of household settled dust via silicon nanomembrane analysis pipeline (SNAP)Environmental Technology & InnovationJune 27, 2025Samantha S. Romanick, Gregory Madejski, Garrett Cashion, Andrew J. Berger, Alison Elder, James McGrathplastic fibers were resuspended in ultrapure water with 0.01 % v/v Tween 20 (Cole, 2016) (Promega, H5152) and stored in glass vials. 2.3. Silicon nanomembrane analysis pipeline (SNAP) 2.3.1. Particle capture

(13) Polysome profiling is an extensible tool for the analysis of bulk protein synthesis, ribosome biogenesis, and the specific steps in translationMolecular Biology of the CellApril 1, 2025Ambar Rodriguez-Martinez, Sara K. Young-Bairdskim milk in 1X TBS (KD Medical, Catalogue No. RGF-3388) with 0.1% Tween 20 (Promega, Catalogue No. H5152) for 1 h. Membranes were incubated at 4°C with rocking overnight in primary antibody diluted in TBST

(14) Diabetes-Mediated STEAP4 Enhances Retinal Oxidative Stress and Impacts the Development of Diabetic RetinopathyAntioxidants (Basel)February 11, 2025Brooklyn E. Taylor, Scott J. Howell, Chieh Lee, Zakary Taylor, Katherine Barber, Patricia R. Tayloranti-Ferritin antibody (Abcam #ab75973, Cambridge, UK) was diluted 1:100 in PBS + 0.05% TWEEN-20 (Promega #H5152, Madison, WI, USA), applied to slides and incubated for 18 h at 4 °C. Slides were washed in 3× PBS +

(15) Mitochondrial DNA removal is essential for sperm development and activityThe EMBO JournalFebruary 11, 2025Zhe Chen, Fan Zhang, Annie Lee, Michaela Yamine, Zong-Heng Wang, Guofeng Zhang, Christian Combs, Hong Xuacetate,Millipore,567422 EGTA,KD Medical,PKE-0460 SSC buffer,Corning,46-020-CM Tween-20,Promega,H5152 Formamide,Sigma,47671 Dextran sulfate,Sigma,S4030 Vanadyl ribonucleoside complex,Sigma,94742 Tetramethylrhodamine

(16) Novel role of zinc-finger protein 518 in heterochromatin formation on α-satellite DNANucleic Acids ResearchJanuary 11, 2025Shinya Ohta, Jun-Ichirou Ohzeki, Nobuko Sato, Hideki Tanizawa, Claire Yik-Lok Chung, Ken-Ichi Noma, Hiroshi Masumoto(#075–00 611, Wako). HAT-MBP-ZNF518B770-987 was extracted using the above buffer with 1% Tween 20 (#H5152, Promega). The target proteins were affinity-purified using Ni-NTA agarose (#143–09 763, Wako). MBP

(17) Optogenetic control of Protein Kinase C-epsilon activity reveals its intrinsic signaling properties with spatiotemporal resolutionbioRxivJanuary 6, 2025Qunxiang Ong, Crystal Jing Yi Yi Lim, Yilie Liao, Justin Tze-Yang Tze-Yang Ng, Ler Ting Rachel Rachel Lim, Shernys Xuan Yi Yi Koh, Sher En En Chan, Pheobe Yu Ying Yu Ying, Huijun Lim, Chen Rui Ye, et al.EASYpack",Roche,4693159001 PhosSTOP™ EASYpack,Roche,4906845001 Triton-X100,Sigma Aldrich,9036–19-5 Tween-20,Promega,H5152 4X LDS,Invitrogen,NP0008 Bovine Serum Albumin (BSA),Capricorn scientific,BSA-1S 10X Tris-Glycine-SDS

(18) Oncogenic PIK3CA corrupts growth factor signaling specificityMolecular Systems BiologyDecember 20, 2024Ralitsa R Madsen, Alix Le Marois, Oliwia N Mruk, Margaritis Voliotis, Shaozhen Yin, Jahangir Sufi, Xiao Qin, Salome J Zhao, Julia Gorczynska, Daniele Morelli, et al.,Thermo Fisher Scientific,#A25742 5X fish skin gelatin blocking agent,Biotium,#22010 "Tween® 20, Molecular Biology Grade",Promega,#H5152 "Triton® X-100, Molecular Biology Grade",Promega,#H5141 Phalloidin iFlouor 555,abcam,#ab176756 CellMaskBlue,Thermo Fisher Scientific

(19) Long-Axial-Range Double-Helix Point Spread Functions for 3D Volumetric Super-Resolution ImagingThe Journal of Physical Chemistry BNovember 21, 2024Yuya Nakatani, Scott Gaumer, Yoav Shechtman, Anna-Karin GustavssonSigma-Aldrich) in PBS for 10 minutes at RT. Next, the cells were permeabilized in 0.5% (v/v) Tween 20 (H5152, Promega) in PBS three times for 5 minutes at RT. The cells were then washed twice in ice-cold PBS and

(20) FRA1 controls acinar cell plasticity during murine Kras(G12D)-induced pancreatic acinar to ductal metaplasiaDevelopmental cellNovember 18, 2024Li AL, Sugiura K, Nishiwaki N, Suzuki K, Sadeghian D, Zhao J, Maitra A, Falvo D, Chandwani R, Pitarresi JR, et al.with 2x Tween-based lysis buffer to make TST (1 ml of 2× ST buffer, 60 µl of 1% Tween-20 (Promega, H5152), 10 µl of 2% BSA (New England Biolabs, B9000S) and 930 µl of nuclease-free water) or with a 2x salt

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