FuGENE HD Transfection Reagent from Promega

Product Specs
ItemFuGENE HD Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2312
Quantity5 x 1ml
Molecular Weight~58kDa
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potencyNat CommunApril 24, 2026Diana Ly, Hyewon Jang, Adhiraj Goel, Arnav Singh, Aditya Raguramtransfer vector plasmid diluted in 1500 µL Opti-MEM with 70 µL of FuGENE HD transfection reagent (Promega; E2312). Two days after transfection, media was centrifuged at 500× g for 5 min to remove cell debris followed

(2) Precise, specific gene editing via a compact GoCas12m-FokI chimeric nucleaseNucleic Acids ResApril 20, 2026Tin Marsic, Sivakrishna Rao Gundra, Mustapha Aouida, Maazallah Masood, Alexander Salibi, Fabian Schmidt, Reem Alquwayzani, Magdy M Mahfouztested for mycoplasma contamination and passaged at 90% confluency. Lipofection using Fugene (Promega – E2312) was performed according to manufacturer’s instructions. Briefly, 8 × 104 cells in 500 µl of antibiotic

(3) Robust Single-molecule Tracking of RNA Abundance and Dynamics via TSSOTiScienceApril 17, 2026Yulin Luo, Yujuan Fu, Jianghu Wang, Sihui Li, Tianyi Cheng, Chengying Pi, Chuhan Qiao, Wei Zou, Nan Liu, Xiaoyue Wang, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, FuGENE HD Transfection Reagent,Promega,E2312 Polyethylenimine,Polysciences,23966 2 × Phanta Max Master Mix (Dye Plus),Vazyme,P525-02 2 × Rapid

(4) Development and Qualification of a Pseudotyped Virus-Based Microneutralisation Assay for Influenza D VirusInfluenza Other Respir VirusesApril 14, 2026Maria Giovanna Marotta, Tobias Mapulanga, Meshach Maina, Janet Daly, Michele Camero, Pauline Mvan Diemen, Helen E. Everett, Emanuele Montomoli, Kelly da Costa, Nigel Temperton, et al.(Thermo Fisher Scientific 1985062, Paisley, UK) and 3.39 μL FuGENE HD transfection reagent (Promega E2312 Madison, USA), added dropwise and followed by incubation for 15 min. The plasmid DNA‐OptiMEM mixture

(5) Characterization of cardiac disease-associated mutations in RyR2 Ca 2+ - and caffeine-binding sitesAmerican Journal of Physiology-Cell PhysiologyApril 1, 2026Venkat R. Chirasani, Akanksha Patwardhan, Naohiro Yamaguchiexpressed in HEK293 cells (Cat. No. CRL-1573, ATCC) using FuGENE HD transfection reagent (Cat. No. E2312, Promega). Since HEK293 cells express endogenous RyR at background level, the heterologous expression

(6) Metabolic control of innate immune activation in TET2-mutant clonal hematopoiesisCell Chem BiolFebruary 19, 2026Peter Geon Kim, Christopher B Hergott, Aidan P Miller, Amy Deik, Meaghan Boileau, Kevin Bullock, Kerry A. Pierce, Angelina H. Choy, Wesley Shin, Marie McConkey, et al.,Selleck Chemicals,Cat#S4163 Biotin,Sigma-Aldrich,Cat#B4501-5G RetroNectin,Takara,Cat#T100B FuGENE 6 transfection reagent,Promega,Cat#E2312 UDP-GlcNAc,Promega,Cat#V7071 OGT peptide substrate,AnaSpec,Cat#63726 Recombinant OGT protein,Antibodies-Online,Cat#ABIN1313360 Recombinant

(7) Programmable and Switchable RNA Scaffolds for Synthetic Condensate Engineering in Mammalian CellsbioRxivFebruary 18, 2026Zhaolin Xue, Oyeshik Mukherjee, Lan Mi, Yizhan Guo, Ru Zheng, Chang Yuan, Mingxu YouD35–20-1.5H) and cultured overnight. Transfections were performed the following day using FuGENE HD (Promega, E2312) according to the manufacturer’s protocol. Briefly, 2.8 μg of total DNA plasmids (1:1 mass ratio for

(8) Cytoplasmic poly-adenosine binding proteins modulate susceptibility of mRNAs to Pumilio-mediated decayNucleic Acids ResFebruary 5, 2026Katherine M McKenney, Carmen Hernandez-Perez, Elise B Dunshee, John M Pum, Anna J DesMarais, Robert P Connacher, Aaron C GoldstrohmTransfections were performed according to the manufacturer’s instructions using 4 μl of Fugene HD (Promega, E2312) per 1 μg of plasmid DNA. Specific transfection conditions are described for each experimental method

(9) Proximity labeling reveals non-catalytic interactions between DPP9 and ubiquitin signaling complexesCell Mol Life SciFebruary 4, 2026Valentina Elena Wirtgen, Layla Saied, Samuel Zolg, Marta Campos Alonso, Bettina Mayer, Laura Donzelli, Ulrich Maurer, H. T. Marc Timmers, Klaus-Peter Knobeloch, Oded Kleifeld, et al.Fluorescence mounting medium,Agilent Dako,S3023 Formaldehyde,Sigma-Aldrich,"104,003" FuGENE HD Transfection Reagent,Promega,E2312 Gibco® DMEM,ThermoFisher,41965-039 Gibco® L-Glutamin,ThermoFisher,25030-024 "Gibco® 0,25% Trypsin

(10) Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potencybioRxivFebruary 2, 2026Diana Ly, Hyewon Jang, Adhiraj Goel, Arnav Singh, Aditya Raguramvector plasmid diluted in 1,500 μL Opti-MEM with 70 μL of FuGENE HD transfection reagent (Promega; E2312). Two days after transfection, media was centrifuged at 500 g for 5 min to remove cell debris followed

(11) Inhibitors supercharge kinase turnover through native proteolytic circuitsNatureJanuary 22, 2026Natalie S. Scholes, Martino Bertoni, Arnau Comajuncosa-Creus, Katharina Kladnik, Xuefei Guo, Fabian Frommelt, Matthias Hinterndorfer, Hlib Razumkov, Polina Prokofeva, Martin P. Schwalm, et al.Nluc-fusion (gift from Promega). Plasmids were transfected into HEK293T cells using FuGENE HD (Promega, E2312), and proteins were allowed to express for 20 h. Serially diluted inhibitor and NanoBRET K4 Tracer (Promega,

(12) ΔNp73 isoform defines a TP53-mutant-like poor-risk subgroup of acute myeloid leukemiaCell Reports MedicineJanuary 20, 2026Diego A. Pereira-Martins, Cesar Ortiz, Isabel Weinhäuser, Albertus T.J. Wierenga, Vincent van den Boom, Fatemeh Mojallali, Dominique Sternadt, Nisha K. van der Meer, Shanna M. Hogeling, Thiago M. Bianco, et al."CD3 MicroBeads, Human",Miltenyi Biotec,130-050-101 MethoCult™,Stemcell,H4435 FuGENE HD Transfection Reagent,Promega,E2312 KAPA RNA HyperPrep Kit with RiboErase (HMR),Roche,08098131702 ,, Deposited data,Deposited data,Deposited

(13) Orthogonal RNA-regulated destabilization domains for three-color RNA imaging with minimal RNA perturbationNature MethodsJanuary 1, 2026Tien G. Pham, Omoyemi Ajayi, Jiaze He, Irina Sagarbarria, Jeanne A. Hardy, Jiahui Wucells from culture flasks. All transfections in this study were carried out using FuGENE HD (Promega, E2312) according to the manufacturer’s recommended protocol. Cell culture media was changed to FluoroBrite™

(14) Development of pyrazolo[1,5-a]pyrimidine based macrocyclic kinase inhibitors targeting AAK1European Journal of Medicinal ChemistryDecember 5, 2025Theresa E. Mensing, Christian G. Kurz, Jennifer A. Amrhein, Theresa A. L. Ehret, Franziska Preuss, Sebastian Mathea, Marwah Karim, Do Hoang Nhu Tran, Zuzana Kadlecova, Tuomas A. Tolvanen, et al.(Greiner 784075) at a density of 2 × 10^5 cells/mL. They were then transfected using FuGENE HD (Promega, E2312). The proteins were allowed to express for 20 h at 37 °C/5% CO2. Using an ECHO acoustic dispenser (Labcyte),

(15) Second-Generation AURKA-Targeting PROTACs: Structural Optimization toward in Vivo Degradation in NeuroblastomaJournal of Medicinal ChemistryNovember 27, 2025Simon Krols, Muhammad Rishfi, Fien Martens, Anouk Van Hauwermeiren, Ellen Sanders, Pieter-Jan De Sutter, An Vermeulen, Kaat De Wever, Sarah-Lee Bekaert, M. Emmy M. Dolman, et al.AURKA’s C-terminus (Promega, #NV1041) were transfected into HEK293T cells using FuGENE HD (Promega, #E2312). Transfected cells were subsequently seeded in 96-well plates (Nunc, #136101) at a density of 20,000

(16) Poly-ubiquitylated transmembrane proteins outcompete other cargo for limited space inside clathrin-coated vesiclesCell ReportsNovember 25, 2025Hao-Yang Liu, Susovan Sarkar, Feng Yuan, Grant Ashby, Carl C. Hayden, Jon M. Huibregtse, Jeanne C. StachowiakSerum,Hyclone,Cat#SH30071.01 Penicillin/Streptomycin/l-glutamine,Hyclone,Cat#16777-169 Hydrocortisone,Sigma,Cat# H4001 Insulin,Sigma,Cat# I6634 HEPES,Fisher Scientific,Cat# BP3101 FuGENE HD,Promega,Cat# E2312 JF646-HaloTag Ligand,Promega,Cat# GA1121 OxyFluor,Oxyrase,Cat# OF-0005 Trypsin-EDTA,Hyclone,Cat#

(17) SARS-CoV-2-ORF3a variant Q57H reduces its pro-apoptotic activity in host cellsF1000ResearchNovember 22, 2025Maria Landherr, Iuliia Polina, Michael W. Cypress, Brian Rhee, Kye-Im Jeon, Sanjana Chandran, Isabel Chaput, Bridget Nieto, Young Min Bae, Bong Sook Jhun, et al.inhibit caspase activation.,43 FuGENE HD,"Promega, Madison, WI, USA and Fugent, LLC, Madison, WI, USA","E2312 and HD-5000, respectively","Used in Figures 1- 5. 0.5-3 μg of plasmids and 7 μl of FuGENE HD were added

(18) Functional analyses and integrated mechanisms of cellular destruction by L-amino acid oxidaseCell Death & DiseaseNovember 20, 2025Krisna Prak, Christin Luft, Eliona Tsefou, Carlos Chávez-Olórtegui, Janos Kriston-Vizi, Robin Ketteler, Vania M. M. BragapcDNA-LAAOY372A using FuGENE® HD transfection reagent at 1 µg plasmid/4 µl transfection reagent (Promega, E2312). Normal human epidermal keratinocytes at 4000 cells/well (passages 3–6) were co-cultured with 3T3-J2

(19) Mapping the placental galectin-3 interactome identifies CD9 and ITGB1 as functional glycoprotein counterreceptors during syncytializationProceedings of the National Academy of SciencesNovember 11, 2025Abigail E. Reeves, Gil-Suk Yang, Sabyasachi Baboo, Jolene K. Diedrich, Pranali Bedekar, Shaheen A. Farhadi, Arun Wanchoo, Christopher Bratcher, Gregory A. Hudalla, John R. Yates, et al.virus glycoprotein (VSV-G), 7.5 µg psPAX2, 10 µg of vector of interest, and 67.5 µL FuGENE HD (Promega, E2312) complexed in 1 mL of Optimem (15 min, RT). Medium was changed 6 h later, replenished with 5 mL of culture

(20) The chordoid glioma PRKCA D463H mutation is a kinase inactive, gain-of-function allele that induces early-onset chondrosarcoma in miceScience SignalingNovember 4, 2025Véronique Calleja, Jack C. Henry, Mathias Cobbaut, Joanne Sewell, Karine Rizzoti, Francesca Houghton, Stefan Boeing, Nneka Anyanwu, Sunita Varsani-Brown, Thomas Snoeks, et al.(Ab1218) was from Abcam. InSolution PMA (phorbol 12-myristate 13-acetate) was from Merck. FuGENE®HD (E2312) was from Promega. cOmplete™, EDTA-free Protease Inhibitor Cocktail (12326400) was from Roche. Streptavidin-HRP conjugate

Be the First to Write a Review!