Akt Control Cell Extracts from Cell Signaling Technology

Product Specs
ItemAkt Control Cell Extracts
CompanyCell Signaling Technology
Price $162.00 Inquire
Catalog Number9273S
Quantity200 µl
SourceCell
Research AreaPI3K / Akt Signaling
TypeCell Extracts
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) STC2 suppresses triple-negative breast cancer migration and invasion by inhibition on EMT and promotion on cell apoptosisDiscover OncologyAugust 9, 2024Ruiwen Lei, Chaoling Yao, Renfeng Huang, Wanming Wu, Linyang Ou, Chuansheng YangSTC2 level, and WB was for pAKT Ser473, pPKCδ Ser645 and pPKCδ Thr507 levels. Rabbit anti-pAKT Ser473 (9273, 1:1000, Cell Signaling Technology, Inc. Shanghai, China), rabbit anti-pPKCδ Ser645 (9376, 1:1000, CST),

(2) Fibre orientation atlas guided rapid segmentation of white matter tractsHuman Brain MappingFebruary 1, 2024Fiona Young, Kristian Aquilina, Kiran K. Seunarine, Laura Mancini, Chris A. Clark, Jonathan D. ClaydenCrP",Right,"144,759","139,019",5740,3.97 CST,"Contamination from: AF/SLF, SFOF, CC, CrP",Total,"293,592","284,319",9273,3.16 AF,Left,"61,922","49,778","12,144",19.61,"Contamination from: EC, CST, CC Overextension into:

(3) Silencing of the ER and Integrative Stress Responses in the Liver of Mice with Error-Prone TranslationCellsOctober 23, 2021James Moore, Ivan Osinnii, Amandine Grimm, Björn Oettinghaus, Anne Eckert, Stephan Frank, Erik C. Böttgergel images are provided in Supplementary Materials Figures S10–S13. Akt control cell extracts (CST, #9273) and HEK 293 cell lysate (lysed in RIPA lysis buffer with protease and phosphatase inhibitors) were

(4) Loss of mitochondrial energetics is associated with poor recovery of muscle function but not mass following disuse atrophyAmerican Journal of Physiology-Endocrinology and MetabolismNovember 1, 2019Michelle B. Trevino et al.(Cambridge, MA). LC3B (cat. no. 3868), S6 (cat. no. 2217), pS6 (cat. no. 2215), phospho-Akt (Ser473, cat. no. 9273), Akt (cat. no. 9272), mTOR (cat. no. 2972), phospho-mTOR (Ser2448, cat. no. 2971), and α-tublin (cat.

(5) Differential YAP nuclear signaling in healthy and dystrophic skeletal muscleAmerican Journal of Physiology-Cell PhysiologyJuly 1, 2019Shama R. Iyer, Sameer B. Shah, Christopher W. Ward, Joseph P. Stains, Espen E. Spangenburg, Eric S. Folker, Richard M. Loveringthe specificity of the phospho-YAP antibody using Jurkat cells treated with calyculin A or LY294002 (9273, Cell Signaling Technologies), in addition to WT and mdx muscle homogenates (Fig. 1E). Calyculin A is

(6) Inhibition of Colorectal Cancer Cell Proliferation by Regulating Platelet-Derived Growth Factor B Signaling with a DNA AptamerAsian Pacific Journal of Cancer PreventionFebruary 26, 2019Suvaraporn Sae-lim, Boonchoy Soontornworajit, Pichayanoot Pichayanootworking dilution of secondary antibody was 1: 10,000 in diluent solution. Akt Control Cell extracts (#9273) were received from Cell Signaling Technology (USA). Cell culture Two types of human colorectal adenocarcinoma

(7) LRIG2 promotes the proliferation and cell cycle progression of glioblastoma cells in vitro and in vivo through enhancing PDGFRβ signalingInternational journal of oncologySeptember 1, 2018Xiao Q, Dong M, Cheng F, Mao F, Zong W, Wu K, Wang H, Xie R, Wang B, Lei T, et al.anti-pAkt rabbit monoclonal antibody 1:1,000 (cat. no. 4058), anti-Akt rabbit polyclonal antibody (cat. no. 9273), anti-pStat3 rabbit monoclonal antibody 1:1,000 (cat. no. 9145), anti-Stat3 rabbit monoclonal antibody

(8) A role for ErbB signaling in the induction of reactive astrogliosisCell DiscoveryDecember 5, 2017Jing Chen, Wanwan He, Xu Hu, Yuwen Shen, Junyan Cao, Zhengdong Wei, Yifei Luan, Li He, Fangdun Jiang, Yanmei Tao

(9) Insulin signaling via the PI3-kinase/Akt pathway regulates airway glucose uptake and barrier function in a CFTR-dependent mannerAmerican journal of physiology. Lung cellular and molecular physiologyMay 1, 2017Molina SA, Moriarty HK, Infield DT, Imhoff BR, Vance RJ, Kim AH, Hansen JM, Hunt WR, Koval M, McCarty NA.treated with either calyculin A or LY-294002 and provided as ready-to-load protein lysate solutions (no. 9273; CST). HeLa and T84 cell line lysates were made in-house. NuLi-1 and CuFi-5 cell lysates were prepared

(10) Increased hypertrophic response with increased mechanical load in skeletal muscles receiving identical activity patternsAmerican Journal of Physiology-Cell PhysiologyOctober 1, 2016Einar Eftestøl, Ingrid M. Egner, Ida G. Lunde, Stian Ellefsen, Tom Andersen, Cecilie Sjåland, Kristian Gundersen, Jo C. Bruusgaardpreviously (109, 128). Positive controls for immunoblotting consisted of Akt Control Cell Extract (9273; Cell Signaling Technology) (data not shown) and HEK-293 cells transfected with an NH2-terminal HAtagged

(11) Tumor suppressive role of sestrin2 during colitis and colon carcinogenesiseLifeFebruary 25, 2016Ro SH, Xue X, Ramakrishnan SK, Cho CS, Namkoong S, Jang I, Semple IA, Ho A, Park HW, Shah YM, et al.S6K (9234), p-Ser235/236 S6 (2211), S6 (2317), p-Thr37/46 4E-BP (2855), 4E-BP (9452), p-Ser473 AKT (9273) and AKT (4691) antibodies from Cell Signaling Technology, Danverse, MA, actin (JLA20) antibody from

(12) Widespread Changes in White Matter Microstructure after a Day of Waking and Sleep DeprivationPLOS ONEMay 28, 2015TorbjÃ¸rn ElvsÃ¥shagen, Linn B. Norbom, Per Ã˜. Pedersen, Sophia H. Quraishi, Atle BjÃ¸rnerud, Ulrik F. Malt, Inge R. Groote, Lars T. Westlyez) maxima Anatomical region of the peak voxela Peak voxel Pvalue FA 21062 " 39, −8, 27 R SLF 0.006 9273 " −23, 31, 19 L ATR, IFOF, UF 0.024 175 " −8, −37, 56 L Cingulum 0.047 64 " −18, 11, −25 L

(13) HIF1A-dependent increase in endothelin 2 levels in granulosa cells: role of hypoxia, LH/cAMP, and reactive oxygen speciesREPRODUCTIONJanuary 1, 2015Ronit Yalu, Adepeju Esther Oyesiji, Iris Eisenberg, Tal Imbar, Rina Meidanendothelin-1 synthesis and the invasive phenotype of ovarian carcinoma cells. Cancer Research 68 9265–9273. (doi:10.1158/ 0008-5472.CAN-08-2093) Rhee SG 2006 Cell signaling. H2O2, a necessary evil for cell signaling. Science

(14) Type I interferons induce autophagy in certain human cancer cell linesAutophagyMay 1, 2013Schmeisser H, Fey SB, Horowitz J, Fischer ER, Balinsky CA, Miyake K, Bekisz J, Snow AL, Zoon KC.(Thr183/Tyr185, 9255S), as well as MAPK8/9 control cell extracts (9253) and AKT control cell extracts (9273) were obtained from Cell Signaling Technology, Inc. Antibodies for detection of SQSTM1 (M153-3) and

(15) Fat Body dSir2 Regulates Muscle Mitochondrial Physiology and Energy Homeostasis Nonautonomously and Mimics the Autonomous Functions of dSir2 in MusclesMolecular and Cellular BiologyJanuary 15, 2013Kushal K. Banerjee, Champakali Ayyub, Samudra Sengupta, Ullas Kolthur-Seetharamanti-phospho-AKT (anti-p-Akt), and antiactin antibodies were purchased from Cell Signaling (catalog numbers 9273 and 4054) and Sigma-Aldrich (catalog number A1978), respectively. Chemiluminescence detection (catalog

(16) Using motor behavior during an early critical period to restore skilled limb movement after damage to the corticospinal motor system during developmentJournal of NeuroscienceJuly 4, 2012Kathleen Friel, Samit Chakrabarty, Hsing-Ching Kuo, John Martinmo- Friel et al. • Restoring Skilled Limb Function J. Neurosci., July 4, 2012 • 32(27):9265–9276 • 9273 Figure 7. CST labeling of axons that originate in the right (contralateral to inactivation) M1 and

(17) Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactionsProteome scienceSeptember 15, 2011Wang X, Dong Y, Jiwani AJ, Zou Y, Pastor J, Kuro-O M, Habib AA, Ruan M, Boothman DA, Yang CR.mutant) cells. The rest of control cell extracts were purchased from Cell Signaling Technology: Akt (cat# 9273), p38 MAP kinase (cat# 9213), NF-κB (cat# 9243), and caspase-3 (cat# 9663). Purified Akt protein was

(18) A growth stimulus is needed for IGF-1 to induce skeletal muscle hypertrophy in vivoJournal of Cell ScienceMarch 15, 2010Thea Shavlakadze, Jinfen Chai, Kirsten Maley, Greg Cozens, Griffin Grounds, Nadine Winn, Nadia Rosenthal, Miranda D. Groundsfrom Cell Signaling, and mouse anti--tubulin (Sigma T5169). Control cell extracts (Cell Signaling 9273 and 9203) were used to test the quality of the antibodies for phosphorylated and total Akt, mTOR and

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