Rabbit Anti-Mouse IgG H&L from Abcam

Product Specs
ItemRabbit Anti-Mouse IgG H&L
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab46540
Quantity1 mg
ApplicationsELISA, WB
Concentration1 mg/ml
IsotypeIgG
HostRabbit
Conjugate/Tag/LabelUnconjugated
Validated ApplicationsWB, ELISA
ClonalityPolyclonal
Antibody TypePrimary Antibody
Research AreaImmunology & Infectious Disease
TargetIghg1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Circadian Controlled Transcription in Brain and Peripheral Organs of Juvenile and Adult MiceInt J Mol SciApril 10, 2026Yasemin Kubra Akyel, Kaan Arslan, Cansu Kose, Aziz Sancarthese were washed with PBS-T, and incubated in secondary antibody, Rabbit-anti-mouse IgG (cat. No. ab46540, Abcam, Cambridge, UK), at room temperature for 1 h. After washing, membranes were developed using the

(2) Acute Depletion of Cited2 in Embryonic Stem Cells Disrupts Gene Networks Controlling Self-Renewal, Homeostasis, and Early Cell Fate CommitmentCellsMarch 3, 2026Leonardo Mendes-Silva, Sara M. Brigida, Marlene Trindade, João M. A. Santos, Lucas Rougier, Rui Machado, Ana Luísa Escapa, Agapios Sachinidis, Jessica L. MacDonald, José BragançaMO, USA) for control immunoprecipitations of anti-H3K27me3 antibody, while a rabbit IgG-ChIP grade (AB46540, Abcam, Cambridge, UK) was used for control immunoprecipitations of anti-H3K27ac. The co-immunoprecipitated

(3) CFP1-mediated H3K4me3 broad domains control early B cell lineage fate determinationNucleic Acids ResearchFebruary 23, 2026Wanyu Bai, Yalin Yang, Yanan Zhao, Shuoxu Gong, Yimiao Lu, Zhonghui Xue, Lie Wang, Fei-Long Meng, Chun Chen, Junchao Dongwere incubated with 0.5 μl of anti-rabbit (Antibodies Online, ABIN101961) or anti-mouse IgG (Abcam, Ab46540) secondary antibodies for 1 h at room temperature. Then cells were washed three times with DIG wash

(4) Integrative Analysis of CUT&Tag-seq and RNA-seq Reveals Widespread H3K27ac Upregulation Associated with the Activation of Muscle-Growth-Related Transcriptional Programs in Broiler Breast MusclePoultry ScienceFebruary 20, 2026Ganxian Cai, Shuang He, Yanyue Pan, Hongyang Zhao, Huaiyu Li, Yuxiang Jiang, Ranran Zhu, Yu Zhang, Ziyi Lian, Zhandeng Li, et al.followed by a 1 h incubation with the appropriate secondary antibody (Proteintech B900210 or Abcam ab46540; 1:50). After washing, cells were incubated with the pG-Tn5 adapter–enzyme complex (1:200 in Dig-300

(5) NSD3 stabilizes nuclear compartmentalization and promotes megabase-scale chromatin interactionsbioRxivFebruary 14, 2026Yi-Hung Chen, John R. Collette, Krupa Sampat, Ivaylo D. Yonchev, Catherine E. Hawkins, Christopher Ponne, Celeste D. Rosencrance, Kyle P. Eagen#N7200, RRID: AB_3665694). Negative control primary antibody was rabbit anti-mouse IgG H&L (Abcam #ab46540). NUT expression is restricted to testis89, therefore we employed the above anti-NUT antibody for BRD4-NUT

(6) Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell StateNat CommunFebruary 12, 2026Yusuke Tsutsui, Atsushi Masui, Satoshi Konishi, Taro Tsujimura, Mio Iwasaki, Takuya Yamamoto, Shimpei Gotohsodium butyrate, 0.05% digitonin, together with 1 µl secondary rabbit anti-mouse IgG antibody (Abcam, ab46540), and was incubated at 4 °C for overnight. After washing the beads with Dig-300 buffer (20 mM HEPES

(7) A prevalent disease-associated SNP in the human ID3 gene regulates E-protein activity and cellular proliferationNAR Molecular MedicineJanuary 16, 2026Christopher A Henderson, Brett P Ransegnola, James Garmey, Ali Khan, Rédouane Aherrahrou, Michael Purdy, Shijie Wu, Andrea Zhou, Jennifer L Kirby, Michael J Lipinski, et al.sc-349X), RNA polymerase II (Abcam Inc. AB5408) as a positive control, or IgG control sera (Abcam Inc. Ab46540) as a negative control. Because this E2A antibody binds to both E12 and E47, we utilized aortic VSMCs

(8) Polycomb misregulation in enterocytes drives tissue decline in the aging Drosophila intestineGenome ResJanuary 1, 2026Sarah M. Leichter, Kami Ahmad, Steven Henikoffanti-RNAPIIS5P (Cell Signaling Technologies 13523, lot 3), anti-Pc (a gift from Judith Kassis), anti-IgG (Abcam ab46540), and anti-GFP (Thermo Fisher Scientific 3E6). Secondary antibodies are as follows: guinea pig α-rabbit

(9) Disparate leukemia mutations converge on nuclear phase-separated condensatesCellDecember 11, 2025Gandhar K. Datar, Elmira Khabusheva, Archish Anand, Joshua Beale, Marwa Sadek, Chun-Wei Chen, Evdokiia Potolitsyna, Nayara Alcantara-Contessoto, Guangyuan Liu, Josephine De La De La Fuente, et al.antibody,BioLegend,Cat#101223 anti-H3K27me3 antibody,Cell Signaling Technology,Cat#9733S mouse IgG antibody,Abcam,Cat#ab46540 anti-CD45 antibody,BD Biosciences,Cat#561864 Bacterial and virus strains,Bacterial and virus strains,Bacterial

(10) Nuclear Argonaute HRDE-1 Maintains Chromatin-Independent Transcriptional Silencing through a Transgenerational Small RNA Feedback LoopbioRxivDecember 2, 2025Arantxa M. L. Rojas, Almira Chervova, Loan Bourdon, Jagannath Jayaraj, Ana Karina Morao, Olivier Feudjio, Sofija Illic, Sara Capurso, Piergiuseppe Quarato, Julie Ovieve, et al.antibodies. We used Guinea pig anti-Rabbit (Antibodies-Online, ABIN101961) or Rabbit anti-mouse (Abcam, ab46540) IgG, according to the source of the primary antibody. Library sequencing. Multiplexed libraries were

(11) Hypoxia drives cervical cancer progression via OCT4/ORAI3-dependent glycolysis and Ca2+ signalingCellular and Molecular Life SciencesNovember 6, 2025Lingjia Lu, Fenfen Wang, Ting Wang, Ziyu Xing, Peiyue Jiangwere incubated with OCT4 antibody (Abcam, Cambridge, MA, USA, ab181557), and the rabbit IgG (Abcam, ab46540) worked as the internal control. Besides, the collected supernatant was the Input control. Then, the

(12) Chromatin context shapes DNA damage formation and nucleotide excision repair dynamics in Caenorhabditis elegansNucleic Acids ResearchNovember 4, 2025Cansu Kose, Cem Azgari, Laura A Lindsey-Boltz, Ogün Adebali, Aziz Sancaras described above, and then incubated in secondary antibody, Rabbit-anti-mouse IgG (Abcam, cat. No. ab46540), at room temperature for 1 h. After washing as described above, membranes were developed using the

(13) Mammalian H4K16ac regulates the spatiotemporal order of genome replication rather than gene expressionNucleic Acids ResearchSeptember 23, 2025Marta Milan, Valeria Runfola, Manthan Patel, Lucia Falbo, Roberta Noberini, Chiara Soriani, Simona Rodighiero, Tiziana Bonaldi, Vincenzo Costanzo, Madapura M Pradeepa, et al.0.5 μg), anti-H3K27me3 (Millipore, 07-449, 5 μg), anti-H3K9me3 (Abcam, ab176916, 5 μg), IgG (Abcam, ab46540, 5 μg). After immunoprecipitation, beads were recovered using a magnet and washed; chromatin was recovered

(14) Genotoxic antibody-drug conjugates combined with Bcl-xL inhibitors enhance therapeutic efficacy in metastatic castration-resistant prostate cancerbioRxivSeptember 16, 2025Galina Semenova, Sander Frank, Ruth Dumpit, Wanting Han, Ilsa Coleman, Roman Gulati, Colm Morrissey, Michael C. Haffner, Peter S. Nelson, John K. Leesections. The sections probed for P53 were washed and incubated with Rabbit Anti-Mouse IgGs (Abcam Cat# ab46540, RRID:AB_2614925, 1:200). DISCOVERY anti-Rabbit HQ secondary antibody (Roche Cat# 760-4815, RRID:AB_2811171)

(15) Mex3a-dependent post-transcriptional silencing ensures olfactory receptor diversity and axon guidance specificityCell ReportsAugust 26, 2025Rachel Duffié, Hani Shayya, Martín Escamilla del Arenal, Miao Wang, Jerome Kahiapo, Aileen Ugurbil, Abdurrahman Keskin, Fiona Clowney, Elizaveta V. Bashkirova, Ariel D. Pourmorady, et al.mouse polyclonal,Abcam,Cat# ab27671; RRID:AB_471093 Anti-mouse IgG – rabbit polyclonal,Abcam,Cat# ab46540; RRID:AB_2614925 PE CD54 – mouse monoclonal,Biolegend,Cat# 322707; RRID:AB_535979 PCNA – chicken polyclonal,Abcam,Cat#

(16) Cancer cells subvert the primate-specific KRAB zinc finger protein ZNF93 to control APOBEC3BProceedings of the National Academy of SciencesAugust 26, 2025Romain Forey, Charlène Raclot, Cyril Pulver, Olga Rosspopoff, Sandra Offner, Julien Duc, Evarist Planet, Filipe Martins, Priscilla Turelli, Didier Trono(Active Motif, AB_2532132), the anti-H3K4me3 primary antibody (C42D8), and anti-rabbit IgG (Abcam, ab46540) secondary antibody. A homemade purified pA-Tn5 protein (3XFlag-pA-Tn5-Fl, Addgene #124601) was produced

(17) Protocol for the spatiotemporal profiling of RNA within nuclear compartments in human cell lines using SLAM-RT&TagSTAR ProtocAugust 9, 2025Nadiya Khyzha, Kami Ahmad, Steven Henikoff(1:100),Antibodies Online,Cat# ABIN101961; RRID: AB_10775589 Rabbit anti-mouse (1:100),Abcam,Cat# ab46540; RRID: AB_2614925 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant

(18) Generation of Hoxa11-3XFLAG and Hoxd11-3XFLAG alleles to investigate Hox11 genome-wide bindingDevelopmental BiologyAugust 1, 2025Martha L. Echevarría-Andino, Jane Y. Song, Paul R. van Ginkel, Shuyang Chen, Corey G.K. Flynn, Sündüz Keleş, Benjamin L. Allen, Deneen M. Wellikantibody (Guinea Pig anti-Rabbit IgG (Antibodies Online, ABIN101961) or Rabbit anti-Mouse IgG (Abcam, ab46540, 1:100) for 30 min at room temperature, followed by 2x washes in Digitonin wash buffer. Next, pAG-MNase

(19) A U3 snoRNA is required for the regulation of chromatin dynamics and antiviral response in Drosophila melanogasterNucleic Acids ResJuly 30, 2025Shruti Jain, Jordi Planells, Isabel Regadas, Donal Barrett, Anne von Euler, Indranil Sinha, Bo Gustav Lindberg, Jenny C Hesson, Patrycja Swacha, Nelson O Gekara, et al.overnight at 4°C using 10 μg/ml of anti-Brg1 antibody and Rabbit IgG as a negative control (Abcam, ab46540). A mix of Protein A and G Dynabeads (Invitrogen) blocked with 1 mg/ml BSA and 1 mg/ml salmon sperm

(20) DynaTag for efficient mapping of transcription factors in low-input samples and at single-cell resolutionNature communicationsJuly 28, 2025Hunold P, Pizzolato G, Heramvand N, Kaiser L, Barbiera G, van Ray O, Thomas R, George J, Peifer M, Hänsel-Hertsch R.antibodies are anti-rabbit IgG antibody (Novus Biologicals, NBP1 72763) or anti-mouse IgG antibody (Abcam, ab46540). A complete list of all chemical reagents is provided as Supplementary Data 1. Nuclei isolation from

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Rabbit Anti-Mouse IgG H&L from Abcam

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Rabbit Anti-Mouse IgG H&L from Abcam

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