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Cell Biology
A Guide to Immunohistochemistry
Blood and Tissue Products
Normal Goat Serum
Normal Goat Serum from Thermo Fisher Scientific
Be the first to write a review!
Citations:
(137)
Supplier Page
Supplier Page from
Thermo Fisher Scientific for
Normal Goat Serum
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Normal Goat Serum
Company
Thermo Fisher Scientific
Price
Supplier Page
Catalog Number
31872
Quantity
120 mg
Applications
Control, Immunocytochemistry, Immunohistochemistry, Inhibition Assays
Format
whole serum diluted in PBS with 0.05% sodium azide; pH 7.6
Isotype
Ig
Concentration
60.0 mg/mL
Species
Goat
Temperature
4° C
Disease
Normal
Storage
4°C
Add to Compare List
Thermo Fisher Scientific
Thermo Fisher Scientific
81 Wyman Street
Waltham
,
MA
02451
United States
Phone
: 800-678-5599
Fax: 800-331-2286
Company Profile
Website:
www.thermofisher.com
Citations (137)
(1)
Mutant KRAS promotes NF-κB driven CCL20 chemokine expression in pancreatic ductal adenocarcinoma
bioRxiv
April 18, 2026
Donovan Drouillard, Marissa Davies, Donna McAllister, Michael B. Dwinell
Burklund Eich Biorepository. Samples were deparaffinized, blocked with 2% (v/v)
goat
serum (
ThermoFisher
#
31872
), stained with mouse anti-human CCL20 (
R&D systems
MAB360
) at a concentration of
1:100
and goat anti-mouse
(2)
Mechanism of ERK-mediated Rho Activation and Stress Fiber Assembly for Cell Migration
bioRxiv
April 17, 2026
Akib M. Khan, Jakaria Shawon, Jared P. Bergman, Keith R. Carney, Michelle C. Mendoza
Lipofectamine™ 2000 Transfection Reagent
,
Invitrogen
,
11668027
HEPES
,
Sigma-Aldrich
,H7006
Normal Goat Serum
,
Invitrogen
,
31872
DBPS,Gibco,14190 DRAQ5,
Thermo Scientific
,
62251
Halt™ protease and phosphatase
inhibitor
cocktail,
Thermo Scientific
(3)
Viral Microglia Reprogramming Clears Oligomeric Neurotoxic Debris
bioRxiv
April 8, 2026
Griffin P. Carter, Zachary P. McKay, Mark A. Katz, Lisbeth Disla, Dasean T. Nardone-White, Derek Southwell, Michael C. Brown, Matthias Gromeier
PBS-T), incubated in blocking buffer (
Donkey
or
Goat Serum
, 10%;
Sigma-Aldrich
#9663 and
Thermo Fisher
#
31872
, respectively) before
incubation
with primary antibody (
1:200
dilution; 24h, 4°C) (antibody probes used
(4)
Advanced CD276-Targeting Dual-Payload Antibody-Drug Conjugates for Cancer Therapy
Cancer Res Commun
April 1, 2026
Zhuoxin Zhou, Davis Ballard, Jiashuai Zhang, Zhantao Du, Natsorn Watcharadulyarat, Tanvi Varadkar, Srijita Chowdhury, Sathvik R. Kambam, Phoebe Hu, Selena Khanal,
et al.
hydrogen peroxide in Tris-buffered saline (TBS) for 10 minutes, followed by 5% normal
goat
serum (cat. #
31872
,
Thermo Fisher Scientific
) in TBS for 1 hour. Our humanized anti-
CD276
mAb was diluted to 1 μg/mL in
(5)
Advanced CD276-Targeting Dual-Payload Antibody–Drug Conjugates for Cancer Therapy
Cancer Res Commun
April 1, 2026
Zhuoxin Zhou, Davis Ballard, Jiashuai Zhang, Zhantao Du, Natsorn Watcharadulyarat, Tanvi Varadkar, Srijita Chowdhury, Sathvik R. Kambam, Phoebe Hu, Selena Khanal,
et al.
hydrogen peroxide in Tris-buffered saline (TBS) for 10 minutes, followed by 5% normal
goat
serum (cat. #
31872
,
Thermo Fisher Scientific
) in TBS for 1 hour. Our humanized anti-
CD276
mAb was diluted to 1 μg/mL in
(6)
FSH and GDF-9 modulate extracellular matrix remodeling in human preantral follicles in a 3D hydrogel culture
Journal of Ovarian Research
March 26, 2026
Arezoo Dadashzadeh, Saeid Moghassemi, Christiani A. Amorim
gentle agitation using PBS containing 0.05%
Triton X-100
(T8787,
Sigma-Aldrich
), 5% normal
goat
serum (
31872
,
ThermoFisher Scientific
), and 0.2% sodium azide (s2002, Sigma-Aldrich). The hydrogels were then incubated
(7)
Exploring roles of neuroglobin in oxygen homeostasis and neurodevelopment based on human cerebral organoid models
iScience
March 20, 2026
Yuan-Hsuan Liu, Meng-Ting Chung, Hsi-Chieh Lin, Hsin-Lei Lin, Hsuan Lin, Ya-Jen Cheng, Hsiao-Mei Wu, Tzyy-Wen Chiou, Yi-Chung Tung
X100 BSA,Sigma-Aldrich,Cat# A7906 Sodium azide,
Sigma-Aldrich
,Cat# S8032
Normal goat serum
,
Thermo Fisher Scientific
,Cat#
31872
Normal donkey serum,Sigma-Aldrich,Cat# D9663
ProLong Gold antifade reagent
,
Thermo Fisher Scientific
,Cat#
(8)
The ontogeny of myeloid-stromal synovial tissue niches in rheumatoid arthritis
bioRxiv
February 10, 2026
Aziza Elmesmari, Domenico Somma, Lucy MacDonald, Jack Frew, Zuzanna Kuczynska, Clara Di Mario, Lavinia Agra Coletto, Shravani Baruah, Denise Campobasso, Theodorous Simakou,
et al.
supplemented with 10% normal serum from the host species of the secondary antibody (e.g.,
goat
serum;
31872
,
ThermoFisher
) to prevent non-specific binding, in a humidified chamber. To assess Collagen Type I (polyclonal)
(9)
Ethanol Alters DNMT1/3a/3b Expression Profile, Promotes Persistent DNA Hypomethylation in Human Brain Endothelial Cells and Impairs Late Cortical Angiogenesis
J Neurochem
February 10, 2026
Michele Siqueira, Matheus Barros, Paula Lacerda Almeida, Luiza Dos Santos Heringer, Henrique Rocha Mendonça, Flávia Carvalho Alcantara Gomes, Joice Stipursky
and incubated in blocking solution containing 3% BSA, 5% normal goat serum (NGS;
Thermo Fisher
, Cat.
31872
), and 0.2% Triton X‐100 in PBS for 1 h. Cells were incubated overnight at 4°C with primary antibodies
(10)
The ontogeny of myeloid-stromal synovial tissue niches in rheumatoid arthritis
Research Square
February 6, 2026
Mariola Kurowska-Stolarska, Domenico Elmesmari, Domenico MacDonald, Lucy MacDonald, Zuzanna Kuczynska, Clara Di Kuczynska, Lavinia Agra Coletto, Shravani Coletto, Shravani Campobasso, Theodorous Campobasso,
et al.
supplemented with 10% normal serum from the host species of the secondary antibody (e.g.,
goat
serum;
31872
,
ThermoFisher
) to prevent non-specific binding, in a humidified chamber. To assess Collagen Type I (polyclonal)
(11)
Glutamatergic Heterogeneity in the Neuropeptide Projections From the Lateral Hypothalamus to the Mouse Olfactory Bulb
Journal of Comparative Neurology
January 1, 2026
Meizhu Qi, Julia Won, Catherine Rodriguez, Douglas A. Storace
(NaBH4),BeanTown Chemical,125800-100G TrueBlack,
Biotium
,
23007
Normal Goat Serum
,
ThermoFisher Scientific
,
31872
Rabbit anti-orexin-A primary antibody (
Ab6214
),
Abcam
,RRID: AB_305380
Mouse
anti-orexin-A
primary antibody
(12)
Photo-proximity labeling in live primary neurons identifies an AMPA-receptor signal transducer in homeostatic synaptic plasticity
Cell Chemical Biology
December 18, 2025
Avik Basu, Yuan Tian, Sergei Kotelnikov, Gabriel M. Cohn, Sadhna Phanse, Dima Kozakov, Heng-Ye Man, Andrew Emili
rinsed three times in PBS. Cells were then blocked with 10%
goat
serum (
Thermo Fisher Scientific
cat. #
31872
) for 1 hour and incubated with properly diluted primary antibodies (in 2.5%
goat
serum/PBS) overnight
(13)
GSDME-dependent pyroptosis drives abdominal aortic aneurysm via promoting vascular senescence
Nat Commun
December 17, 2025
Si-Jia Sun, Zhen Zhang, Guo-Yan Zhang, Jian-Jin Wu, Yi-Xin Zhang, Wen-Bin Wu, Yan Zhang, Hang Yin, Jian-Bin Zhang, Qi Cao,
et al.
quenched with 1% H2O2 for 20 min and the unspecific binding was blocked with 5% normal
goat
serum (#
31872
,
Thermo
) for 2 h. Then the sections were stained with primary antibodies at room temperature for 4 h.
(14)
Combined inhibition of NAD synthesis and C-terminal binding protein cooperatively induces cell death and inhibits growth of high grade serous ovarian carcinoma
Scientific Reports
December 6, 2025
Kranthi Kumar Chougoni, Jacqueline West, Nicholette St. Martin, Diana Dcona, Istem Kose, Nari Kim, Martin M. Dcona, Ronny Drapkin, Joseph W. Carlson, Ann E. Walts,
et al.
Retriever 2100 (Aptum Biologics). Slides were blocked in 5%
Goat serum
(
Thermo Fisher Scientific
, Cat No:
31872
) for 1 h at room temperature and incubated with primary antibodies to
CtBP1
(
mouse
anti-
CtBP1
,
1:25
;
(15)
Fosl1 is a transcriptional effector of BRAF V600E-driven intestinal tumorigenesis
iScience
November 21, 2025
Zakia Alam, Rebecca Nightingale, Analia Lesmana, Cheng Liu, Laura J. Jenkins, Mark F. Richardson, Lawrence Croft, Ian Y. Luk, Camilla M. Reehorst, Fiona Chionh,
et al.
system",
Agilent Technologies
Australia,Cat#
K346811-2
Normal goat serum
,
Thermo Fisher Scientific
,Cat#
31872
Triton™ X-100
,
Sigma Aldrich
,Cat# X100-100 ML DMEM-F12,
Thermo Fisher Scientific
,Cat#
11320033
L-glutamine
,
Thermo Fisher Scientific
(16)
Adaptable Immunofluorescence Protocol for Muscle Fiber Typing in FFPE Human and Mouse Skeletal Muscle and Intact Mouse Hindlimbs
Current Protocols
November 3, 2025
Connor Thomas, Lainey M. Hibbard, Kenneth E. White, Steven S. Welc
15–33. Reagents and Solutions Blocking Buffer 250 µl
Normal Goat Serum
(5% v/v final;
Invitrogen
, cat. no.
31872
)4.75 ml PBS 0.25%
Triton‐X 100
(95% v/v final;
Millipore Sigma
, cat. no. EM‐TX1568‐1)Prepare fresh and
(17)
Depot-specific roles for sphingosine kinase 1 in conversion of white adipose to thermogenic adipose
bioRxiv
October 22, 2025
Yolander Valentine, Maryam Jamil, Anna Kovilakath, Ryan D. R. Brown, Jordon Dail, Saaid Farooq, Sarah Spiegel, L Ashley Cowart
minutes, 3 times. Tissue sections were then covered with a layer of PBS containing 5% normal
goat
serum (#
31872
,
ThermoFisher
), 1% BSA and 0.4% Triton X-100 (buffer A) for 1 hour at room temperature to simultaneously
(18)
Controlled Delivery of a Neurotrophic Factor in the Adult Mouse Brain Using Engineered Microglia
bioRxiv
October 10, 2025
Rohan J. Hofland, Marta Grońska-Pęski, Hiroko Nobuta, Nicolas Buitrago, Karan Malhotra, Jean M. Hébert, J. Tiago Gonçalves
solution for 30 minutes. Wells were filled with blocking solution: 3 mg/mL normal
goat
serum (
ThermoFisher
31872
), 0.1mg/mL bovine serum albumin (Fisher BP9700100), 0.3% Triton X-100 in 1X PBS for 1 hour, followed
(19)
Fetal rat neural progenitor cell transplantation after spinal cord injury improves motor recovery following optogenetic stimulation
Molecular Therapy
October 1, 2025
María del Mar Sánchez-Martín, Esther Giraldo, Ana Alastrue-Agudo, Eric López-Mocholi, Samuel Martín-Pérez, Loris Maninno, Guillem Paniagua Soriano, Ana Isabel Fraga Sánchez, Javier Monreal-Trigo, José Manuel Terrés-Haro,
et al.
per slice.
Immunocytochemistry
Cells were permeabilized and blocked with 5% normal goat serum (NGS;
31872
,
Thermo Fisher
) and 0.2%
Triton X-100
(9036-19-5,
Sigma
). Cells were incubated overnight at 4°C with
(20)
ADSL deficiency is a secondary mitochondrial disease affecting organelle homeostasis and ERK2/AKT signaling in a linear genotype-phenotype relation
Cell Reports
September 23, 2025
Matteo Bordi, Beatrice Testa, Claudia Compagnucci, Fiorella Colasuonno, Francesca Cipressa, Elisabetta Betterini, Andrea Mancini, Claudia Carsetti, Illari Salvatori, Caterina Ferraina,
et al.
solution of primary antibodies diluted in PBS and 3% Normal Goat Serum (NGS) (
Thermo Fisher Scientific
#
31872
) O/N at 4°C. Cells were incubated with a solution of secondary fluorescent antibodies anti-rabbit IgG
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Normal Goat Serum from Thermo Fisher Scientific
Supplier Page
Product Specs
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