Description
Mouse C1 Inhibitor Genetically Deficient Brain Tissue Lysate is prepared from a C1 Inhibitor Homozygous Knockout Mouse. This C1 Inhibitor Homozygous Knockout was created by targeted excision of the SERPING1 gene. Homozygous mice completely lack C1 Inhibitor protein expression. Organ is flash-frozen immediately upon removal and stored frozen until lysed. The tissue is then lyophilized and thorougly homogenized in Radio Immuno Precipitation Assay (RIPA) buffer containing protease and phosphate incubators. The final homogenate is then incubated with DNase, clarified via centrifugation (10,000g at 4 degree C) and stored at-80 degree C. Each lot is tested for protein concentration via BCA assay and analyzed via SDS-PAGE to confirm the presence of sharp, clear bands as an indicator of quality