Transcriptor Reverse Transcriptase from Roche

Product Specs
ItemTranscriptor Reverse Transcriptase
CompanyRoche
Price Get Pricing Get Quote for this Product
Catalog Number03531287001
Quantity2,000 U (4 x 500 U) (200 reactions)

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Transcriptional Assessment of Striatal mRNAs as Valid Biomarkers of Disease Progression in Three Mouse Models of Huntington’s DiseaseJournal of Huntington's DiseaseMarch 3, 2026Afshin Ghavami, Michael Olsen, Mei Kwan, Jose Beltran, John Shea, Sylvie Ramboz, Wenzhen Duan, Daniel Lavery, David Howland, Larry C. ParkIX Transcriptor Reverse Transcription reaction buffer and Transcriptor Reverse Transcriptase (Cat # 03531287001, Roche Applied Science, Indianapolis, IN). Reverse transcription reactions were performed in triplicate

(2) Potential compensatory mechanisms preserving cardiac function in myotubular myopathyCellular and Molecular Life SciencesDecember 3, 2024Alix Simon, Nadège Diedhiou, David Reiss, Marie Goret, Erwan Grandgirard, Jocelyn Laportesynthesised from 1 µg of RNA by reverse transcription using Transcriptase inverse Transcriptor (Roche, 03531287001), according to the manufacturer’s protocol. Quantitative RT-PCR analysis was performed with gene-specific

(3) Potential Role of APOBEC3 Family Proteins in SARS-CoV-2 ReplicationVirusesJuly 16, 2024MST Monira Begum, Ayub Bokani, Samiul Alam Rajib, Mohadeseh Soleimanpour, Yosuke Maeda, Kazuhisa Yoshimura, Yorifumi Satou, Diako Ebrahimi, Terumasa IkedaFG-81250), and cDNA was synthesized by Transcriptor Reverse Transcriptase (Roche, Basel, Switzerland, Cat# 03531287001) with random hexamer. RT-qPCR was performed using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific,

(4) Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targetsSTAR protocolsSeptember 15, 2023Seigneurin-Berny D, Touat-Todeschini L, Timcheva K, Verdel A.SDS,Euromedex,Cat#BI-SD8119 Spermidin,Sigma,Cat#S0266 Sucrose,Sigma,Cat#S7903 Transcriptor reverse transcriptase,Roche,Cat#03531287001 Trypsin-EDTA (0.05%),Thermo Fisher,Cat#25300054 TRIS Base,Euromedex,Cat#EU1018-A Triton X-100,Sigma,Cat#T9284 TRIzol Reagent

(5) APOBEC3 degradation is the primary function of HIV-1 Vif determining virion infectivity in the myeloid cell line THP-1mBioAugust 31, 2023Ikeda T, Shimizu R, Nasser H, Carpenter MA, Cheng AZ, Brown WL, Sauter D, Harris RS.Genetics, Cat# FG-81250). Then, cDNA was synthesized by Transcriptor Reverse Transcriptase (Roche, Cat# 03531287001) and used to amplify A3C or A3H gene with the following primers: [A3C outer primers: (5´-GCG CTT CAG

(6) APOBEC3 degradation is the primary function of HIV-1 Vif for virus replication in the myeloid cell line THP-1bioRxiv : the preprint server for biologyMarch 29, 2023Ikeda T, Shimizu R, Nasser H, Carpenter MA, Cheng AZ, Brown WL, Sauter D, Harris RS.Genetics, Cat# FG-81250). Then, cDNA was synthesized by Transcriptor Reverse Transcriptase (Roche, Cat# 03531287001) and used to amplify A3C or A3H gene with the following primers [A3C outer primers: (5’-GCG CTT CAG

(7) SHERLOCK4HAT: A CRISPR-based tool kit for diagnosis of Human African TrypanosomiasiseBioMedicineNovember 1, 2022NÃºria Sima, Annick Dujeancourt-Henry, Blanca Liliana Perlaza, Marie-Noelle Ungeheuer, Brice Rotureau, Lucy Glovereach RPA primer (240 nM for TgSGP RPA primers), Reaction buffer 1×, 2.2 U of Transcriptor (Roche; Ref 03531287001), 1.5 U of Murine RNase inhibitor (NEB; Ref M0314L) and 14 mM MgOAc and 0.22 pellet of TwistAmp Basic

(8) SUPT3H-less SAGA coactivator can assemble and function without significantly perturbing RNA polymerase II transcription in mammalian cellsNucleic Acids ResearchAugust 12, 2022Veronique Fischer, Vincent Hisler, Elisabeth Scheer, Elisabeth Lata, Bastien Morlet, Damien Plassard, Dominique Helmlinger, Didier Devys, László Tora, Stéphane D Vincentprimers (ThermoFisher Scientific, Cat# SO142) and 10 U Transcriptor Reverse Transcriptase (Roche, Cat# 03531287001) following manufacturer's instructions. qPCR For qPCR, the cDNA samples were amplified using LightCycler®

(9) Callose-Regulated Symplastic Communication Coordinates Symbiotic Root Nodule DevelopmentSSRN Electronic JournalMarch 31, 2022Rocio Gaudioso-Pedraza, Martina Beck, Lisa Frances, Carolina Ripodas, Andreas Niebel, Giles E. D. Oldroyd, Yoselin Benitez-Alfonso, Fernanda de Carvalho-NiebelReagents Agilent Technologies Cat#5067-1511 Transcriptor Reverse Transcriptase for QRT-PCR Roche Cat#03531287001 LightCycler480 Sybr Green I Master Roche Cat#04707516001 Anilin Blue EMS Cat#10840; CAS: 28631-66-5 glutaraldehyde

(10) miR-21 mimic blocks obesity in mice: A novel therapeutic optionMolecular therapy. Nucleic acidsDecember 3, 2021Lhamyani S, Gentile AM, Giráldez-Pérez RM, Feijóo-Cuaresma M, Romero-Zerbo SY, Clemente-Postigo M, Zayed H, Olivera WO, Bermúdez-Silva FJ, Salas J, et al.2, or 4 μg of total RNA was converted to cDNA using RT (Transcriptor Reverse Transcriptase 20 U/μL, 03531287001; Roche). Next, qRT-PCR amplification was performed using specific TaqMan Gene Expression Assays, 10

(11) Glial‐derived neurotrophic factor in human airway smooth muscleJournal of Cellular PhysiologyDecember 1, 2021Sangeeta Bhallamudi, Benjamin B. Roos, Jacob J. Teske, Sarah A. Wicher, Andrea McConico, Christina M. Pabelick, Venkatachalem Sathish, Y. S. PrakashMD, USA; 74104), cDNA synthesized (Transcriptor Reverse Transcriptase; Roche, Indianapolis, IN, USA; 03531287001), and Quantitative Real Time PCR performed (Roche Light Cycler 96 System) using SYBR Green PCR Master

(12) The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanismsCell ReportsAugust 24, 2021Veronique Fischer, Damien Plassard, Tao Ye, Bernardo Reina-San-Martin, Matthieu Stierle, Laszlo Tora, Didier Devyshexamer primers (ThermoFisher Scientific, Cat#SO142) and Transcriptor Reverse Transcriptase (Roche, Cat#03531287001) following manufacturer’s instruction. For qPCR, the cDNA samples were amplified using LightCycler®

(13) A MicroRNA Linking Human Positive Selection and Metabolic DisordersCellOctober 29, 2020Wang L, Sinnott-Armstrong N, Wagschal A, Wark AR, Camporez JP, Perry RJ, Ji F, Sohn Y, Oh J, Wu S, et al.Assays Dual-Luciferase® Reporter,Promega,E1910 Assay System,, Transcriptor reverse transcriptase,Roche,Cat# 03531287001 RNeasy Plus Mini Kit,QIAGEN,Cat# 74134 Pierce BCA Protein Assay Kit,Thermo Scientific,Cat# 23225 Seahorse

(14) Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-γ and TNF-α in intestinal epithelial cellsCell death & diseaseJanuary 27, 2020Woznicki JA, Flood P, Bustamante-Garrido M, Stamou P, Moloney G, Fanning A, Zulquernain SA, McCarthy J, Shanahan F, Melgar S, et al.

(15) Asymmetric distribution of glucose transporter mRNA provides a growth advantage in yeastThe EMBO JournalMay 15, 2019Timo Stahl, Stefan Hümmer, Nikolaus Ehrenfeuchter, Nitish Mittal, Geoffrey Fucile, Anne Spangof total RNA was reversely transcribed with the Transcriptor reverse transcriptase kit (Roche, Cat# 03531287001), oligo-dTs, and random hexamers. The mRNA levels were analyzed by SYBR green incorporation using the

(16) A multicenter comparison of quantification methods for antisense oligonucleotide-induced DMD exon 51 skipping in Duchenne muscular dystrophy cell culturesPLOS ONEOctober 2, 2018Monika Hiller et al.Verheul et al. [30]. cDNA was generated from 750 ng of RNA with Transcriptor Reverse Tanscriptase (#03531287001, Roche), random hexamer primers (#11034731001, Sigma-Aldrich), RNasin ribonuclease inhibitor (#N2115,

(17) Tracking disease progression non-invasively in Duchenne and Becker muscular dystrophiesJournal of Cachexia, Sarcopenia and MuscleAugust 1, 2018Pietro Spitali et al.RNase‐free water. cDNA synthesis was performed using the Transcriptor Reverse Transcriptase (cat. n. 03531287001, Roche), random hexamers (cat. n. SO142, ThermoFisher Scientific), RNAsin ribonuclease inhibitor (cat.

(18) Distinct retinoic acid receptor (RAR) isotypes control differentiation of embryonal carcinoma cells to dopaminergic or striatopallidal medium spiny neuronsScientific reportsOctober 20, 2017Podleśny-Drabiniok A, Sobska J, de Lera AR, Gołembiowska K, Kamińska K, Dollé P, Cebrat M, Krężel W.the manufacturer’s protocol. Thereafter, cDNA was synthesized with the Transcriptor Kit (Roche, ref. 03531287001). cDNA was diluted 10 times and polymerase chain reaction (PCR) was performed using SybrGreen (Qiagen,

(19) Expression profiles and function analysis of microRNAs in postovulatory aging mouse oocytesAgingApril 1, 2017Wang TY, Zhang J, Zhu J, Lian HY, Yuan HJ, Gao M, Luo MJ, Tan JH.heated at 65°C for 10 min followed by quenching on ice. Then 4 μl 5× RT buffer, 0.5 μl RNase inhibitor (03531287001, Roche), 2 μl 10 mM dNTP (R0192, Fermentas), 2 μl 10 μM stem-loop reverse transcription primer (Table

(20) Neovascular deterioration, impaired NADPH oxidase and inflammatory cytokine expression in adipose-derived multipotent cells from subjects with metabolic syndromeMetabolismMarch 23, 2017Wilfredo Oliva-Olivera, Said Lhamyani, Leticia Coín-Aragüez, Daniel Castellano-Castillo, Juan Alcaide Torres, Elena María Yubero-Serrano, Rajaa El Bekay, Francisco José Tinahones

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Roche's Transcriptor Reverse Transcriptase
Transcriptor RT is a new recombinant reverse transcriptase with a number of useful features. The ... Read More

Transcriptor Reverse Transcriptase from Roche

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Roche's Transcriptor Reverse Transcriptase

Transcriptor Reverse Transcriptase from Roche

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