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Thermostable Reverse Transcriptase
Transcriptor Reverse Transcriptase
Transcriptor Reverse Transcriptase from Roche
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Citations:
(29)
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Roche for
Transcriptor Reverse Transcriptase
Supplier Page
Description
A recombinant reverse transcriptase for the robust transcription of RNA fragments up to 14 kb used in two-step RT-PCR on thermal cyclers and real-time PCR instruments.
For life science research only. Not for use in diagnostic procedures.
Product Specs
Company Info
Citations
Reviews
Product Reviews
Product Specs
Item
Transcriptor Reverse Transcriptase
Company
Roche
Price
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Get Quote for this Product
Catalog Number
03531287001
Quantity
2,000 U (4 x 500 U) (200 reactions)
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Roche
Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road
Indianapolis
,
Indiana
United States
Phone
: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911
Company Profile
Website:
sequencing.roche.com
Citations (29)
(21)
Stress-induced alternative splice forms of MDM2 and MDMX modulate the p53-pathway in distinct ways
PloS one
January 1, 2014
Jacob AG, Singh RK, Comiskey DF Jr, Rouhier MF, Mohammad F, Bebee TW, Chandler DS.
in reverse transcription reactions that were carried out using the Transcriptor RT enzyme (Catalog no.
03531287001
,
Roche Diagnostics
, Indianapolis, IN) according to manufacturer’s instructions. For detection of
miRNA
(22)
APOBEC3B Upregulation and Genomic Mutation Patterns in Serous Ovarian Carcinoma
Cancer Research
December 15, 2013
Brandon Leonard et al.
(
Qiagen
, cat#
74106
). cDNA was prepared using the
Transcriptor Reverse Transcriptase
Kit (
Roche
, cat#
03531287001
) and
qPCR
was performed using 2x Probes Master Mix (
Roche
, cat #
04887301001
). All primer and probe combinations
(23)
CXXC5 (Retinoid-Inducible Nuclear Factor, RINF) is a Potential Therapeutic Target in High-Risk Human Acute Myeloid Leukemia
Oncotarget
August 15, 2013
Audrey Astori et al.
volume using oligo-dT primers with
Transcriptor Reverse Transcriptase
(
Roche
, Basel, Switzerland, Cat. N°
03531287001
) in accordance with the manufacturer's instructions. Quantitative PCRs (
qPCR
) were carried out using
(24)
Comprehensive Behavioral and Molecular Characterization of a New Knock-In Mouse Model of Huntington’s Disease: zQ175
PLoS ONE
December 20, 2012
Liliana B. Menalled et al.
Transcriptor Reverse Transcription reaction buffer and 10U
Transcriptor Reverse Transcriptase
(Cat #
03531287001
,
Roche Applied Science
, Indianapolis, IN) in 20 µL total volume. The reactions were allowed to proceed
(25)
Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal
Journal of Oncology
August 25, 2010
Hironobu Yamashita, Manisha Tripathi, Jerome Jourquin, Yoonseok Kam, Shanshan Liu, Brandy Weidow, Vito Quaranta
(26)
APOBEC3 degradation is the primary function of HIV-1 Vif determining virion infectivity in the myeloid cell line THP-1
mBio
January 1, 0001
"Ikeda T, Shimizu R, Nasser H, Carpenter MA, Cheng AZ, Brown WL, Sauter D, Harris RS."
Genetics, Cat# FG-81250). Then, cDNA was synthesized by
Transcriptor Reverse Transcriptase
(
Roche
, Cat#
03531287001
) and used to amplify A3C or
A3H
gene with the following primers: [A3C outer primers: (5´-GCG CTT CAG
(27)
APOBEC3 degradation is the primary function of HIV-1 Vif for virus replication in the myeloid cell line THP-1
bioRxiv : the preprint server for biology
January 1, 0001
"Ikeda T, Shimizu R, Nasser H, Carpenter MA, Cheng AZ, Brown WL, Sauter D, Harris RS."
Genetics, Cat# FG-81250). Then, cDNA was synthesized by
Transcriptor Reverse Transcriptase
(
Roche
, Cat#
03531287001
) and used to amplify
A3C
or
A3H
gene with the following primers [
A3C
outer primers: (5’-GCG CTT CAG
(28)
A MicroRNA Linking Human Positive Selection and Metabolic Disorders
Cell
January 1, 0001
"Wang L, Sinnott-Armstrong N, Wagschal A, Wark AR, Camporez JP, Perry RJ, Ji F, Sohn Y, Oh J, Wu S,
et al
."
Assays
Dual-Luciferase® Reporter,
Promega
,
E1910
Assay
System,,
Transcriptor reverse transcriptase
,
Roche
,Cat#
03531287001
RNeasy Plus Mini Kit,
QIAGEN
,Cat#
74134
Pierce BCA Protein Assay Kit
,
Thermo Scientific
,Cat#
23225
Seahorse
(29)
miR-21 mimic blocks obesity in mice: A novel therapeutic option
Molecular therapy. Nucleic acids
January 1, 0001
"Lhamyani S, Gentile AM, Giráldez-Pérez RM, Feijóo-Cuaresma M, Romero-Zerbo SY, Clemente-Postigo M, Zayed H, Olivera WO, Bermúdez-Silva FJ, Salas J,
et al
."
2, or 4 μg of total RNA was converted to cDNA using RT (
Transcriptor Reverse Transcriptase
20 U/μL,
03531287001
;
Roche
). Next,
qRT-PCR
amplification
was performed using specific TaqMan
Gene Expression
Assays
, 10
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Roche's Transcriptor Reverse Transcriptase
Transcriptor RT is a new recombinant reverse transcriptase with a number of useful features. The ...
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Transcriptor Reverse Transcriptase from Roche
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