Biometra TRIO Thermal Cycler Series from Analytik Jena

Product Specs
ItemBiometra TRIO Thermal Cycler Series
CompanyAnalytik Jena
Price Get Pricing Get Quote for this Product
Catalog NumberBiometra TRIO
QuantityEA
Temperature Range3°C to 99°C
Heating RateUp to 5°C/s
Cooling RateUp to 4.5°C/s
Temperature Uniformity95°C ± 0.60°C after 15 s
70°C ± 0.30°C after 15 s
55°C ± 0.20°C after 15 s
Display7" color touchscreen
Electrical Requirements115V and 230V models available
ApplicationsPCR
Wells48-well
Lid TypeHigh-Performance Smart Lid technology
Lid Temperature Range 30°C to 110°C
Dimensions(WxDxH) 300 mm x 410 x 250 mm
CapacityBiometra TRIO 30 : 3 blocks for 30 x 0.5 ml tubes
Biometra TRIO 48 : 3 blocks for 48 x 0.2 ml tubes or 48 well microplates or 6 x strips of 8
Biometra TRIO combi : 3 combi blocks for 18 x 0.5 ml tubes or 48 x 0.2 ml tubes or 48 well microplates or 6 x strips of 8

Features

3 independent, interchangeable blocks, Temperature gradient

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Local Office

Analytik Jena

3 Highwood Drive

Suite 103E

Tewksbury, MA 01876

United States

Phone: 1.800.452.6788

Email: info@us.analytik-jena.com

Company Profile

Website: https://www.analytik-jena.us/

International Office

Analytik Jena

Konrad-Zuse-Strasse 1

07745 Jena

Germany

Phone: +49 3641 77 7444

Company Profile

Website: https://www.analytik-jena.com/

(1) Integrated workflow for discovery of microprotein-coding small open reading framesSTAR ProtocolsDecember 1, 2023Kevin Cao, Yasamin Hajy Heydary, Gregory Tong, Thomas Farid Martinez"Novex™ TBE-Urea Gels, 10%, 10 well",Invitrogen,Cat# EC6875BOX "Novex™ TBE Gels, 8%, 10 well",Invitrogen,Cat# EC6215BOX Biometra TRIO 48 Endpoint Thermal Cycler,Analytik Jena,Cat# 846-2-070-720 Eppendorf ThermoMixer® C,Eppendorf,Cat#

(2) A Multiplex RT-PCR Method for the Detection of Reptarenavirus InfectionVirusesNovember 25, 2023Francesca Baggio, Udo Hetzel, Barbara Prähauser, Eva Dervas, Eleni Michalopoulou, Tanja Thiele, Anja Kipar, Jussi Hepojokireaction) were used for each 20 µL total volume multiplex RT-PCR reaction. Reactions were run on a Biometra trio thermocycler (Analytik Jena, Jena, Germany) with a three-step protocol with the following cycling conditions:

(3) mRNA Abundance of Neurogenic Factors Correlates with Hearing Capacity in Auditory Brainstem Nuclei of the RatLife (Basel)September 2, 2023Jonas Engert, Julia Doll, Barbara Vona, Totta Ehret Kasemo, Bjoern Spahn, Rudolf Hagen, Kristen Rak, Johannes Voelkerwith 500 ng RNA, Buffer GE, and RNase-free water per reaction. This was incubated for 5 min at 42 °C (Biometra Trio 30, Analytik Jena®, Jena, Germany) and then placed on ice for 1 min. Then, 10 µL reverse transcriptase

(4) Stabilizing Polymer Coatings Alter the Protein Corona of DNA Origami and Can Be Engineered to Bias the Cellular UptakeACS Polymers AuAugust 9, 2023Hugo J. Rodríguez-Franco, Jorieke Weiden, Maartje M. C. Bastingsin a total volume of 50 μL. Folding was performed using a thermal annealing ramp in a thermocycler (Biometra TRIO Analytik Jena) (80 °C for 5 min, 60 to 20 °C at −1 °C per hour). DONs were subsequently purified from

(5) First virological and pathological study of Göttingen Minipigs with Dippity Pig Syndrome (DPS)PLOS ONEJune 15, 2023Hina Jhelum, Nanna Grand, Kirsten Rosenmay Jacobsen, Sabrina Halecker, Michelle Salerno, Robert Prate, Luise Krüger, Yannick Kristiansen, Ludwig Krabben, Lars Möller, et al.carried out with AmpliTaq DNA Polymerase (Applied Biosystems, Waltham, MA, USA) and were set up with a Biometra TRIO cycler (Analytik Jena, Jena, Germany). The PCR approach for the short amplicon size was applied with

(6) DNA methylation protocol for analyzing cell-free DNA in the spent culture medium of human preimplantation embryosSTAR ProtocolsJune 1, 2023Yuan Gao, Yidong Chen, Jie Qiao, Jin Huang, Lu WenAnalytical Technologies,FSv2-CE Thermomixer,Eppendorf,5355 000.011 PCR thermocycler,Analytik Jena,Biometra TRIO Sequencer,Illumina,NovaSeq 6000 Low-binding filter tips 10 μL,QSP,TF104-10-Q Low-binding filter tips

(7) Protocol for in vivo and in vitro study of lymphatic valve formation driven by shear stress signaling pathwaySTAR ProtocolsJune 1, 2023Richa Banerjee, Luz A. Knauer, Ying YangDrybath Stdrd heating block,Fisherbrand,Cat#88860023 Centrifuge 5430R,Eppendorf,Cat#5428IG027636 Biometra TRIO 48,Analytik jena,Cat#8462070723 ChemiDoc™ XRS+ system,Bio-Rad,Cat#1708265 Vari-mix™ test tube rocker,ThermoScientific,Cat#12-815-3Q Stereomicroscope

(8) One to one comparison of cell-free synthesized erythropoietin conjugates modified with linear polyglycerol and polyethylene glycolScientific ReportsApril 19, 2023Paria Pouyan, Anne Zemella, Jeffrey L. Schloßhauer, Ruben M. Walter, Rainer Haag, Stefan Kubickprecipitated with isopropanol and resuspended in ultra-pure water. tRNA was folded in a PCR cycler (Biometra TRIO, Analytik Jena) and stored at − 80 °C. Cell-free synthesis of EPO Cell-free protein synthesis was

(9) Advanced Skin Antisepsis: Application of UVA-Cleavable Hydroxyethyl Starch Nanocapsules for Improved Eradication of Hair Follicle-Associated MicroorganismsPharmaceuticsFebruary 11, 2023Loris Busch, Anna Maria Hanuschik, Yuri Avlasevich, Katrin Darm, Elisa F. Hochheiser, Christian Kohler, Evgeny A. Idelevich, Karsten Becker, Peter Rotsch, Katharina Landfester, et al.hotstart II DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA) kit. PCR was performed on a Biometra TRIO 48 thermocycler (Analytik Jena GmbH, Jena, Germany) using the following conditions: initial incubation

(10) Molecular Features and Antimicrobial Susceptibilities of Streptococcus equi ssp. equi Isolates from Strangles Cases in IndonesiaVeterinary SciencesJanuary 10, 2023Dordia Anindita Rotinsulu, Christa Ewers, Katharina Kerner, Amrozi Amrozi, Retno Damayanti Soejoedono, Torsten Semmler, Rolf Bauerfeind(Thermo Fisher Scientific), 19.8 μL sterile deionised water and 3 μL of the template. PCR was performed on a Biometra TRIO 48 thermal cycler (Analytik Jena, Jena, Germany) using the following protocol: 94 °C for 5 min (initial

(11) Expedited retrieval of high-quality Usutu virus genomes via Nanopore sequencing with and without target enrichmentFrontiers in MicrobiologyNovember 9, 2022Cora M. Holicki, Felicitas Bergmann, Franziska Stoek, Ansgar Schulz, Martin H. Groschup, Ute Ziegler, Balal SadeghiSuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) and the multi-block PCR thermal cycler Biometra TRIO (Analytik Jena GmbH, Jena, Germany) were used. A purification was performed using gel electrophoresis

(12) Synthesis of an Anti-CD7 Recombinant Immunotoxin Based on PE24 in CHO and E. coli Cell-Free SystemsInternational journal of molecular sciencesNovember 8, 2022Krebs SK, Stech M, Jorde F, Rakotoarinoro N, Ramm F, Marinoff S, Bahrke S, Danielczyk A, Wüstenhagen DA, Kubick S.annealing temperature of 60 °C and for 35 cycles, according to manufacturer’s recommendations, in a Biometra TRIO thermocycler (Analytik Jena, Jena, Germany). A total of 1 µL of the PCR reaction was analyzed by electrophoresis

(13) Accumulation patterns of intracellular salts in a new halophilic amoeboflagellate, Euplaesiobystra salpumilio sp. nov., (Heterolobosea; Discoba) under hypersaline conditionsFrontiers in MicrobiologyAugust 5, 2022Hyeon Been Lee, Dong Hyuk Jeong, Jong Soo Parkof double distilled water, and 2 μl of extracted DNA template. DNA amplification was performed on a Biometra TRIO thermal cycler (Analytik Jena, Jena, Germany) with the PCR steps as follows: an initial denaturing step

(14) A RAS-Independent Biomarker Panel to Reliably Predict Response to MEK Inhibition in Colorectal CancerCancers (Basel)July 1, 2022Ulrike Pfohl et al.primer annealing at 58 °C for 30 s, and extension at 72 °C for 30 s in an automated DNA thermal cycler (Biometra TRIO Thermal Cycler Series, Analytik Jena AG, Jena, Germany). The final extension was performed at 72 °C for 5 min. The PCR products,

(15) A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv FunctionalityFrontiers in Bioengineering and BiotechnologyApril 29, 2022Simon K. Krebs, Nathanaël Rakotoarinoro, Marlitt Stech, Anne Zemella, Stefan KubickUnited States) was added to the DNA fragments, mixed by pipetting, and incubated for 30 min at 50°C in a Biometra TRIO thermocycler (Analytik Jena, Jena, Germany). The reactions were purified using DNA Clean and Concentrator-5

(16) Virological Characterization of Pigs with Erythema MultiformeMicroorganismsMarch 18, 2022Sabrina Halecker, Vasileios Papatsiros, Dimitra Psalla, Ludwig Krabben, Benedikt Kaufer, Joachim DennerUSA) was used in a PCR reaction volume of 20 μL plus 5 μL DNA template. The PCR was carried out on a Biometra TRIO cycler (Analytik Jena, Jena, Germany). 2.6. Testing of PCMV, PLHV and PCV For the molecular biological

(17) First experiences with the Spectrum Compact CE SystemInternational Journal of Legal MedicineJanuary 1, 2022Nastasja Burgardt, Melanie Weissenbergeraccording to those measurements. PCR, CE and data analysis All PCR amplifications were carried out on a Biometra Trio 30 (Analytik Jena, Jena, Germany). For the autosomal Qiagen kits (Investigator® ESSplex SE QS, Investigator®

(18) SARS-CoV-2 infection triggers profibrotic macrophage responses and lung fibrosisCellDecember 22, 2021Daniel Wendisch et al.3′ Reagent Kit v3.1 provided by Biolegend, including primer sequences and reagent specifications. A Biometra Trio Thermal Cycler was used for amplification and incubation steps (Analytik Jena). Libraries were quantified

(19) Virological and Parasitological Characterization of Mini-LEWE Minipigs Using Improved Screening Methods and an Overview of Data on Various Minipig BreedsMicroorganismsDecember 18, 2021Sabrina Halecker, Julia Metzger, Christina Strube, Ludwig Krabben, Benedikt Kaufer, Joachim DennerMA, USA) and 0.5 µL of 10 mM dNTP Mix (Thermo Fisher Scientific, Waltham, MA, USA) and applied on the Biometra TRIO cycler (Analytik Jena, Jena, Germany). To detect PERV-A/C [38], a PCR was performed in a reaction volume

(20) Dynamic changes in genetic diversity, drug resistance mutations, and treatment outcomes of falciparum malaria from the low-transmission to the pre-elimination phase on the islands of São Tomé and PríncipeMalaria JournalDecember 14, 2021Ying-An Chen, Tsen-Ju Shiu, Lien-Fen Tseng, Chien-Fu Cheng, Wei-Liang Shih, Arlindo Vicente de Assunção Carvalho, Kun-Hsien Tsaiat 50 °C for 30 s, extension at 72 °C for 30 s, and a final extension at 72 °C for 10 min using the Biometra TRIO Thermal Cycler (Analytik Jena AG, Jena, Germany). The fragment lengths of the amplified products varied

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8/29/2018An Excellent Multiblock PCR Machine Sonali MunshawThe research group mainly focus on DNA damage and repair. Part of our studies involve using transgenic animal model.This involves regular genotyping so we can use right animal for the studies.It is ... Read Review