TBE Buffer, 10X, Molecular Biology Grade from Promega

Product Specs
ItemTBE Buffer, 10X, Molecular Biology Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV4251
TypeBuffer
Quantity1,000ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The use of an avian macrophage-like cell line (HD11) transduced with recombinant lentiviruses to dissect the immunomodulatory role of Marek’s disease virus gene productsVirus GenesJune 1, 2026Sonsiray Alvarez-Narvaez, Steven J. Conrad, Taejoong Kim, Stephen Spatz, John R. DunnS2) on a 1% UltraPure Agarose (Invitrogen, catalog no. 16500) in 1X TBE buffer (Promega, catalog no. V4251) gel, and visualized in an Alphaimager Model 2 (Alpha Innotech). Amplicon sizes were compared to a Promega

(2) Protocol for the identification of recent HIV infection in newly diagnosed individualsSTAR ProtocMay 13, 2026Wahyu Nawang Wulan, Dewi Lokida, Evy Yunihastuti, Herman Kosasih, Dona Arlinda, Chuen-Yen Lau, Aaron Neal, Pratiwi P. Sudarmono, Frank MaldarelliAM9937 "Agarose, LE, Analytical Grade",Promega,Cat# V3121 "TBE Buffer, 10×, Molecular Biology Grade",Promega,Cat# V4251 "Sigma-Aldrich Ethyl alcohol, Pure",Merck,Cat# E7023-500ML QIAamp Viral RNA Mini Kit,Qiagen,Cat# 52906 SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

(3) Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activationSci AdvJanuary 1, 2026Zhao Wang, Wei Dong, Xiangling Feng, Xinran Xu, Yao An, Huiling Xiao, Menghan Luo, Qian Liang, Ruoxuan Bai, Ke Zhao, et al.electrophoresed on 6% native polyacrylamide gels for 1.5 hours at 100 V in 0.5× TBE buffer (Promega, V4251) and transferred to an Immobilon-NY+ Nylon Membrane (Millipore, INYC00010) for 30 min at 350 mA. The

(4) Molecular Detection of natural bacterial flora within Culex pipiens pipiens (Diptera: Culicidae) in Aleppo, SyriaJournal of Genetic Engineering and BiotechnologyDecember 1, 2025Ahmed Allahem, Reem Alajmi, Mais Alzarzor Alajami, Sumaiah Al-Ghamdi, Muhammad Amjad Bashir, Jehan Zeb, Khaloud Mohammed Alarjani, Nawal M Al-Malahi, Rewaida Abdel-GaberElectrophoresis Electrophoresis was performed in a 1 × TBE (Tris/Borate/EDTA buffer) (from Promega Corporation, Cat. V4251, USA) running buffer on 1.5 % agarose gel (from Gene DirecX, Cat No: MB755-0100, USA) supplemented with

(5) Isolation of chromium resistant bacteria from tannery waste and assessment of their chromium reducing capabilities - A Bioremediation ApproachHeliyonMarch 30, 2024Roksana Khanam, Sheikh Abdullah Al Ashik, Umme Suriea, Shahin MahmudOrigin: Promega, USA); ethidium bromide solution (Cat: H5041, Origin: Promega, USA); TAE Buffer (Cat: V4251, Origin: Promega, USA) reagents were used. Finally gel documentation was done by alpha imager (Model:

(6) Continuous irrigation with thrombolytics for intraventricular hemorrhage: case–control studyNeurosurgical ReviewJanuary 10, 2024Diego A. Carrera, Marc C. Mabray, Michel T. Torbey, Jason E. Andrada, Danika E. Nelson, Preeyaporn Sarangarm, Heather Spader, Chad D. Cole, Andrew P. Carlson26] Briefly, we mixed agar (Sigma – USA, Cat No. A7921) with 1x TBE buffer (Promega - USA. Cat No. V4251) at a concentration of 0.6% w/v and allowed it to boil for 5 minutes while mixing. When the mixture

(7) Rfoot‐seq: Transcriptomic RNase Footprinting for Mapping Stable RNA‐Protein Complexes and Rapid Ribosome ProfilingCurrent ProtocolsNovember 20, 2023Qianru Li, Emily K. Stroup, Zhe Jiagarose gel (precast, see Reagents and Solutions) TBE (10×), molecular biology grade, (Promega, cat. no. V4251) 6x Gel loading dye (New England BioLabs, cat. no. B7024S) DsDNA markers for library size selection

(8) Development of a multiassay algorithm (MAA) to identify recent HIV infection in newly diagnosed individuals in IndonesiaiScienceOctober 20, 2023Wahyu Nawang Wulan, Evy Yunihastuti, Dona Arlinda, Tuti Parwati Merati, Rudi Wisaksana, Dewi Lokida, Zehava Grossman, Kristi Huik, Chuen-Yen Lau, Nugroho Harry Susanto, et al.AM9937 "Agarose, LE, Analytical Grade",Promega,Cat# V3121 "TBE Buffer, 10X, Molecular Biology Grade",Promega,Cat# V4251 "Sigma-Aldrich Ethyl alcohol, Pure",Merck,Cat# E7023-500ML ,, Critical commercial assays,Critical

(9) Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seqSTAR ProtocolsSeptember 1, 2023Isabel Cristina Vélez-Bermúdez, Shu-Jen Chou, Ai-Ping Chen, Wen-Dar Lin, Wolfgang Schmidt"Formamide, molecular biology grade",Promega,Cat#H5051 Bromophenol blue,Bio-Rad,Cat#161-0404 "TBE buffer, 10×, molecular biology grade",Promega,Cat#V4251 "10,000× SYBR Gold",Invitrogen,Cat#S11494 Ultra Low Range DNA Ladder,Invitrogen,Cat#10597012 GlycoBlue coprecipitant (15 mg/mL

(10) First report on molecular identification of Fusarium species causing fruit rot of mandarin ( Citrus reticulata) in BangladeshF1000ResearchFebruary 15, 2023Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath SikdarPromega, USA) gel with 0.5% ethidium bromide solution (Cat # H5041, Promega, USA) in 1x TAE buffer (Cat # V4251, Promega, USA) using a 1kb DNA ladder (Cat # G5711, Promega, USA) as marker and visualized under alpha

(11) First report of Serratia marcescens associated with black rot of Citrus sinensis fruit , and evaluation of its biological control measures in BangladeshF1000ResearchMarch 4, 2022Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath SikdarProtein Simple, USA) with 0.5% ethidium bromide solution (Cat: H5041; Promega, USA) in 1xTAE buffer (Cat: V4251; Promega, USA) using a1kb DNA ladder (Cat: G5711; Promega, USA). PCR products were purified from the

(12) First report of Serratia marcescens associated with black rot of Citrus sinensis fruit , and evaluation of its biological control measures in BangladeshF1000ResearchNovember 26, 2020Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath SikdarProtein Simple, USA) with 0.5% ethidium bromide solution (Cat: H5041; Promega, USA) in 1xTAE buffer (Cat: V4251; Promega, USA) using a1kb DNA ladder (Cat: G5711; Promega, USA). PCR products were purified from the

(13) First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in BangladeshF1000ResearchOctober 8, 2020Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath SikdarPromega, USA) gel with 0.5% ethidium bromide solution (Cat # H5041, Promega, USA) in 1x TAE buffer (Cat # V4251, Promega, USA) using a 1kb DNA ladder (Cat # G5711, Promega, USA) as marker and visualized under alpha

(14) Transcriptome-wide measurement of translation by ribosome profilingMethodsAugust 1, 2017Nicholas J. McGlincy, Nicholas T. Ingoliaµg/µl,Invitrogen,10821015,– miRNA marker,New England Biolabs,N2102S,– 10× TBE RNase-free,Promega,V4251,– 10000× SYBR Gold,Invitrogen,S11494,10 10% SDS molecular biology grade,Promega,V6551,7 5′ Adenylation

(15) Electrical characterization of DNA supported on nitrocellulose membranesScientific ReportsJuly 12, 2016Mahmoud Al Ahmad, Reham M. Milhem, Neena G. Panicker, Tahir A. Rizvi, Farah MustafaTris-base (T6066, Sigma-Aldrich, USA), EDTA (E-5134, Sigma-Aldrich, USA), Tris/Borate/EDTA (10XTBE) buffer (V4251, Promega, USA), 6X loading dye (13526, Norgen, Canada), 100 bp ladder (CSL-MDNA-100 bp, Wolf Laboratories,

(16) Binding kinetics of DNA-protein interaction using surface plasmon resonanceProtocol ExchangeMay 22, 2013Bongsup Cho, V. G. Vaidyanathan, L. Xu, Bongsup P. Cho\(Sigma, cat. No. A9418) 40%, 19:1 Acrylamide/Bis \(Bio-Rad, cat. No. 1610144) TBE \(Promega, cat. No. V4251) Urea \(Fisher Scientific, cat. No. 104924) Ammonium persulfate \ (APS) \(Sigma, cat. No. A3426) TEMED

(17) The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragmentsNature ProtocolsAugust 1, 2012Nicholas T. Ingolia, Gloria A. Brar, Silvia Rouskin, Anna M. McGeachy, Jonathan S. Weissmanphosphorylation. Note that all residues are ribonucleotides.10x TBE, RNase-free (Promega, cat. no. V4251)10,000x SYBR Gold (Invitrogen, cat. no. S11494) [CAUTION – Nucleic acid stains are typically mutagenic.

(18) Longitudinal disease studies in small-holder black tiger shrimp ( Penaeus monodon) ponds in Andhra Pradesh, India. II. Multiple WSSV genotypes associated with disease outbreaks in ponds seeded with uninfected postlarvaeAquacultureJune 15, 2011Peter J. Walker, Nicholas Gudkovs, C.V. Mohan, V. Stalin Raj, Balakrishnan Pradeep, Evan Sergeant, A.B. Chandra Mohan, G. Ravibabu, Indrani Karunasagar, T.C. Santiago

(19) An autoimmune pleiotropic SNP modulates IRF5 alternative promoter usage through ZBTB3-mediated chromatin loopingNature communicationsJanuary 1, 0001"Wang Z, Liang Q, Qian X, Hu B, Zheng Z, Wang J, Hu Y, Bao Z, Zhao K, Zhou Y, et al."were electrophoresed on 6% native polyacrylamide gels for 1.5 h at 100 V in 0.5× TBE buffer (Promega, V4251), and then transferred to an Immobilon-NY+ Nylon Membrane (Millipore, INYC00010) for 30 min at 380 mA.

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