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Taq Polymerase (Recombinant)
GoTaq DNA Polymerase
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GoTaq DNA Polymerase
GoTaq DNA Polymerase
GoTaq DNA Polymerase from Promega
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Citations:
(27)
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GoTaq DNA Polymerase
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Description
Taq DNA Polymerase. Provided with Green and Colorless Reaction Buffers. Green Buffer allows direct gel analysis after amplification. Enhanced performance over standard Taq DNA Polymerase
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
GoTaq DNA Polymerase
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
M3008
Quantity
2,500u
Concentration
5 U/µl
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (27)
(1)
HTLV-1 HBZ inhibits DHX9 to reprogram circRNA biogenesis in ATLL
Tumour Virus Res
July 20, 2026
Julien Ladet, Mateo Bazire, Nicolas Fontrodona, Anne Van den Broeke, Ambroise Marçais, Mariam Shallak, Greta Forlani, Roberto S. Accolla, Cyril F. Bourgeois, Franck Mortreux
(7.5 ng) was used as a template for
PCR amplification
in a reaction tube containing GoTaq polymerase (
M3008
,
Promega
).
PCR
products were separated by
agarose gel electrophoresis
stained with ethidium bromide
(2)
Elucidating the protein interaction network of one of the largest icosahedral capsids in the virosphere
The EMBO Journal
April 10, 2026
Hela Safi, Alain Schmitt, Alwena Tollec, Lucid Belmudes, Agathe M G Colmant, Olivier Poirot, Sebastien Santini, Matthieu Legendre, Yohann Couté, Hugo Bisio,
et al.
,
Thermo Fisher
,
F530L
In Fusion Snap Assembly,Takara,638948 Terra
PCR
Direct RedDye Premix,Takara,639286 GoTaq,
Promega
,
M3008
NotI-HF
,
New England Biolabs
(
NEB
),
R3189S
EcoRI-HF
,
New England Biolabs
(
NEB
),
R0101S
HindIII-HF
,
New England Biolabs
(3)
Cranial neural crest shortage leads to extensive craniofacial anomalies in mice mutant for the NR2F1/2 nuclear receptors
Developmental Biology
February 1, 2026
David Paulding, Simon J. Y. Han, Jonathan Timmons, Michelle Caye, Alexa Riedel, Samantha A. Brugmann, Lindsey Barske
and migration (labeled as 4–5ss).
PCR
genotyping
was carried out using
GoTaq DNA Polymerase
(
Promega
M3008
) on ear or tail clippings from
mouse
pups and
amnion
and/or tail tissue from embryos, extracted using
(4)
Three Staphylococcus Bacteriophages Isolated from Swine Farm Environment in Quebec, Canada, Infecting S. chromogenes
Viruses
January 22, 2026
Mousumi Sarker Chhanda, Rébecca E. St-Laurent, Valérie E. Paquet, Nicolas Deslauriers, Cynthia Gagné-Thivierge, Martine Denicourt, Marie-Ève Lambert, Antony T. Vincent, Steve J. Charette
prepared as previously described [29].
PCR amplification
was carried out using
GoTaq® DNA polymerase
(PR
M3008
,
Promega Corporation
, Fitchburg, WI, USA) and the RAPD #18 primer (5′-GCCAGCAGG-3′) [30]. The thermal
(5)
Usp21 Knockout Causes Abnormal Lipid Metabolism in Mouse and Its Polymorphism Correlates with Hypercholesterolemia in Outpatients
Int J Mol Sci
October 6, 2025
Sailakshmi Iyer, Naoko Hattori, Hiroshi Okuda, Takeya Nakagawa, Satoshi Fujii, Takahiro Maeda, Haruhiko Koseki, Takashi Ito
SNP are mentioned in Table S4. The
PCR
was performed using the
GoTaq DNA polymerase
(
Promega
Cat.No.
M3008
, Madison, WI, USA). The
PCR master mix
included 5xGoTaq buffer 2 μL, 2.5 mM dNTP 0.8 μL, 10μM forward
(6)
Use of HSALR female mice as a model for the study of myotonic dystrophy type I
Lab Anim (NY)
April 1, 2025
Marc Carrascosa-Sàez, Anna Colom-Rodrigo, Irene González-Martínez, Raquel Pérez-Gómez, Andrea García-Rey, Diego Piqueras-Losilla, Ana Ballestar, Beatriz Llamusí, Estefanía Cerro-Herreros, Ruben Artero
splicing was assayed by
standard PCR
, using 20 ng of cDNA as template, GoTaq polymerase (
Promega
, cat. no.
M3008
) and specific primers for Atp2a1, Nfix, Clcn1, Mbnl1, Bin1 and Cacna1s alternative splicing events,
(7)
Multiple Immune and Genetic Mechanisms Contribute to Cmv5s-Driven Susceptibility and Tissue Damage during Acute Murine Cytomegalovirus Infection
The Journal of Immunology
March 1, 2024
Jessica L. Annis, John Benjamin W. Duncan, Helen O. Billcheck, Anna G. Kuzma, Rowena B. Crittenden, Michael G. Brown
isolated from toe and/or tail clips from pre-weanling pups.
PCR
was run using the
Promega
GoTaq® kit (Cat.
M3008
) reagents, dNTPs purchased from Genesee Scientific (Cat. 42–410), EvaGreen® dye purchased from Biotium
(8)
Recombinant Attenuated Edwardsiella piscicida Vaccine Displaying Regulated Lysis to Confer Biological Containment and Protect Catfish against Edwardsiellosis
Vaccines (Basel)
September 9, 2023
Banikalyan Swain, Vanessa A. Campodonico, Roy Curtiss
were used to carry out
cloning
. To perform all
PCR reactions
,
GoTaq DNA polymerase
(
Promega
, catalog#
M3008
) was used. To perform plasmid DNA isolation and the
purification
of gel fragments and
PCR
products,
(9)
High density SNP array and reanalysis of genome sequencing uncovers CNVs associated with neurodevelopmental disorders in KOLF2.1J iPSCs
bioRxiv
July 27, 2023
Carolina Gracia-Diaz, Jonathan E. Perdomo, Munir E. Khan, Brianna Disanza, Gregory G. Cajka, Sunyimeng Lei, Alyssa Gagne, Jean Ann Maguire, Thomas Roule, Ophir Shalem,
et al.
(
Invitrogen
, #
18427013
), 0.5 µM forward and 0.5 µM reverse primers,
GoTaq DNA Polymerase
(
Promega
, #
M3008
) and 1µL of template gDNA. To prepare
PCR
products for
Sanger Sequencing
we enzymatically purified the
(10)
Large-scale F0 CRISPR screens in vivo using MIC-Drop
Nature Protocols
June 1, 2023
Saba Parvez, Zachary J. Brandt, Randall T. Peterson
Setup)
Uracil-DNA glycosylase (UDG
) (
New England Biolabs
, cat. no.
M0280S
)
GoTaq DNA polymerase
(
Promega
, cat. no.
M3008
)Exonuclease I (New England Biolabs, cat. no. M0293)Shrimp alkaline phosphatase (
New England Biolabs
,
(11)
Construction and Evaluation of Recombinant Attenuated Edwardsiella piscicida Vaccine (RAEV) Vector System Encoding Ichthyophthirius multifiliis (Ich) Antigen IAG52B
Frontiers in Immunology
January 25, 2022
Banikalyan Swain, Cole T. Powell, Roy Curtiss
and T4 ligase were used for
cloning
. For all
PCR reactions
,
GoTaq DNA polymerase
(
Promega
, catalog#
M3008
) was used. For plasmid DNA isolation and
purification
of gel fragments and
PCR
products, Qiagen products
(12)
A simple one-step PCR assay for SNP detection
MicroPubl Biol
June 11, 2021
Jian Chen, Tim Schedl
systems. Methods All primers designed to have Tm between 58 °C to 61 °C.
GoTaq DNA Polymerase
(
Promega
, cat#
M3008
) was used in all
PCR reactions
described, following the manufacturer’s instructions. The single worm
(13)
A Salmonella Typhi RNA thermosensor regulates virulence factors and innate immune evasion in response to host temperature
PLoS pathogens
March 1, 2021
Brewer SM, Twittenhoff C, Kortmann J, Brubaker SW, Honeycutt J, Massis LM, Pham THM, Narberhaus F, Monack DM.
10 minutes before being used as the DNA template in
PCR reactions
using GoTaq polymerase (
Promega
#
M3008
) and primers oSMB64/65 and oSMB75/76 (see S3 Table).
PCR
products were run on a 0.9% agarose TAE gel
(14)
Bone Morphogenic Proteins Are Immunoregulatory Cytokines Controlling FOXP3
+
T
reg
Cells
Cell reports
October 6, 2020
Browning LM, Miller C, Kuczma M, Pietrzak M, Jing Y, Rempala G, Muranski P, Ignatowicz L, Kraj P.
SuperScript IV First-Strand Synthesis System
,
Thermo Fisher Scientific
,Cat#
18091050
GoTaq DNA polymerse with buffer,
Promega
,Cat#
M3008
,, Deposited Data,, ,,
RNA-seq
,This paper,GEO: GSE103124 ,,
Experimental Models
: Organisms/
Strains
,
Experimental Models
(15)
A Rapid, SuperSelective Method for Detection of Single Nucleotide Variants in
Caenorhabditis elegans
Genetics
October 1, 2020
Denis Touroutine, Jessica E. Tanis
shown). Gradient
PCR
was performed by aliquoting a master mix containing
GoTaq DNA Polymerase
(
Promega
Cat. No.
M3008
), 500 nM of each primer, and 5 ng of clean gDNA into 15 µl reactions. Instead of 1 µl clean gDNA, 1
(16)
TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis
Cancers
July 8, 2020
Chan YL, Lai WC, Chen JS, Tseng JT, Chuang PC, Jou J, Lee CT, Sun HS.
expression of TIAM2S transcripts was examined by
PCR
to amplify 352-bp amplicons using
GoTaq DNA polymerase
(
M3008
,
Promega
, Madison, WI, USA) with its buffer and dNTP (forward primer 5′-CTTCTGCCCCATTAAACGAA-3′, reverse
(17)
Pancreatic cancer organoids recapitulate disease and allow personalized drug screening
Proceedings of the National Academy of Sciences of the United States of America
December 26, 2019
Driehuis E, van Hoeck A, Moore K, Kolders S, Francies HE, Gulersonmez MC, Stigter ECA, Burgering B, Geurts V, Gracanin A,
et al
.
5′-ACACGTCTGCAGTCAACTGG-3′ and reverse primer 5′- TAACTTGAAACCCAAGGTAC-3′, with
GoTaq DNA Polymerase
(
Promega
;
M3008
) according to protocol at an annealing temperature of 58 °C. A 1% Agarose gel was used to purify the
(18)
Does the widely distributed rodent Calomys tener (Cricetidae: Sigmodontinae) constitute a single evolutionary unit?
Zoologia
May 28, 2019
Raul E. Gonzalez-Ittig, Narayan P. Kandel, Cibele R. Bonvicino, Jorge Salazar-Bravo
mixtures were set at 25μL total volume with 1 μL of DNA (20–30 ng/μL), 0.13 μL of
Promega
Taq (Cat#
M3008
, 5U/μL), 0.8 μL of dNTPs mix (Cat#U151B, 10mM), 2 μL of MgCL2 (25 mM), 2.5 μL of 5 × Go Taq Green buffer,
(19)
Cas4-Dependent Prespacer Processing Ensures High-Fidelity Programming of CRISPR Arrays
Molecular Cell
April 1, 2018
Hayun Lee, Yi Zhou, David W. Taylor, Dipali G. Sashital
,
New England Biolabs
,#
R3101L
T4 Polynucleotide Kinase
,
New England Biolabs
,#
M0201L
GoTaq DNA polymerase
,
Promega
,#
M3008
DpnI
,
New England Biolabs
,#
R0176L
Agar,AMRESCO,#J637-1kg
Carbenicillin disodium salt
,RPI,#C46000-25.0
Kanamycin monosulfate
(20)
Human cardiomyocytes for drug discovery
Vigilância Sanitária em Debate
February 28, 2018
Diogo Gonçalves Biagi et al.
samples were diluted for 10 ng/mL. For the endpoint
PCR
were used
Promega
’s GoTaq DNA Polimerase #
m3008
and for
quantitative PCR
was used Power SyBer Green Master Mix
PCR
#4367659. Primers sequences are available
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GoTaq DNA Polymerase from Promega
Supplier Page
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