T7 RNA Polymerase (HC) from Promega

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100µl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl2,10mM DTT, 10mM NaCl2, 2mM spermidine, 0.05% Tween®-20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5µCi [3H]CTP and 2µg supercoiled pGEM®-5Zf(+) Vector DNA.