T7 DNA Polymerase (unmodified) from New England Biolabs

Product Specs
ItemT7 DNA Polymerase (unmodified)
CompanyNew England Biolabs
Price $306.00 Inquire
Catalog NumberM0274L
Concentration10,000 units/ml
Quantity1500 units
ApplicationsDNA Manipulation
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of deoxynucleotides into acid-insoluble material in a total reaction volume of 50 µl in 30 minutes at 37°C in 1X T7 DNA Polymerase Reaction Buffer with 150 µM dNTPs including [³H]-dTTP, 0.5 mM activated calf thymus DNA and 50 µg/ml BSA.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) RNA polymerase II is a polar roadblock to a progressing DNA forkNature CommunicationsSeptember 29, 2025Taryn M. Kay, James T. Inman, Lucyna Lubkowska, Tung T. Le, Jin Qian, Porter M. Hall, Sahil Batra, Dirk Remus, Dong Wang, Mikhail Kashlev, et al.flash-frozen for storage. Wild-type T7 DNA polymerase was purchased from New England Biolabs (NEB, M0274S). E. coli thioredoxin (trx) was purchased from Sigma-Aldrich (Sigma, T0910). RNase T1 was purchased

(2) Optimized metrics for orthogonal combinatorial CRISPR screensScientific reportsMay 6, 2023Cetin R, Wegner M, Luwisch L, Saud S, Achmedov T, Süsser S, Vera-Guapi A, Müller K, Matthess Y, Quandt E, et al.T4 DNA ligase (New England Biolabs, M0202) and 30 units of T7 DNA polymerase (New England Biolabs, M0274) were added. This reaction was incubated overnight at room temperature. 3Cs-reaction was purified by

(3) Shelterin Components Modulate Nucleic Acids Condensation and Phase Separation in the Context of Telomeric DNAJournal of Molecular BiologyAugust 1, 2022Andrea Soranno, J. Jeremías Incicco, Paolo De Bona, Eric J. Tomko, Eric A. Galburt, Alex S. Holehouse, Roberto Gallettosplit by polarization[103]. λDNA was biotinylated by T7 DNA polymerase in T7 polymerase buffer (NEB M0274) for 40’ at 12 °C in the presence of 0.6 mM dNTPs (dATP, dTTP, and dCTP) and 1 mM biotinylated-dGTP,

(4) A Structure-Based Mechanism for DNA Entry into the Cohesin RingMolecular CellSeptember 17, 2020Torahiko L. Higashi et al.AcTEV protease,ThermoFisher,Cat# 12575015 PstI-HF,New England BioLabs,Cat# R3140S T7 DNA polymerase,New England BioLabs,Cat# M0274S CloneAmp HiFi PCR Premix,TaKaRa,Cat# 639298 GoTaq Taq G2 DNA Polymerase,Promega,Cat# M7845 Deoxynucleotide

(5) Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNABIO-PROTOCOLJanuary 5, 2020Martin Wegner, Koraljka Husnjak, Manuel Kaulichnumber: M0202), storage -20 °C 10. T7 DNA polymerase (unmodified) (New England Biolabs, catalog number: M0274), storage -20 °C 11. Restriction enzyme I-SceI (New England Biolabs, catalog number: R0694), storage

(6) DNA Polymerase Delta Synthesizes Both Strands during Break-Induced ReplicationMolecular CellNovember 1, 2019Roberto A. Donnianni, Zhi-Xiong Zhou, Scott A. Lujan, Amr Al-Zain, Valerie Garcia, Eleanor Glancy, Adam B. Burkholder, Thomas A. Kunkel, Lorraine S. SymingtonPhosphatase,NEB,Cat# M0371 RNase HII,NEB,Cat# M0288 T4 RNA Ligase I,NEB,Cat# M0204 T7 DNA Polymerase,NEB,Cat# M0274 Zymolyase 20T,USBiological,Cat# 37340-57-1 Proteinase K,Roche,Cat# 03115852001 Critical Commercial

(7) Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genomeeLifeMarch 6, 2019Wegner M, Diehl V, Bittl V, de Bruyn R, Wiechmann S, Matthess Y, Hebel M, Hayes MG, Schaubeck S, Benner C, et al.100 mM dNTP mix (Roth, 0178.1/2), T4 DNA ligase (NEB, M0202), T7 DNA polymerase (unmodified) (NEB, M0274), Thermo Fisher Scientific GeneJET Gel Extraction Kit (Thermo Fisher, K0692), 3M sodium acetate (Sigma-Aldrich,

(8) A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface ATPNature communicationsFebruary 12, 2019Lobas MA, Tao R, Nagai J, Kronschläger MT, Borden PM, Marvin JS, Looger LL, Khakh BS.µl 10× T4 DNA ligase buffer, 1 µl 25 mM dNTPs, 1 µl 10 mM ATP, 1 µl T7 polymerase unmodified (NEB: M0274L), 1 µl T4 ligase (NEB: M0202M), and the entire 10 µl annealing reaction was added to a PCR tube and

(9) Evidence that DNA polymerase δ contributes to initiating leading strand DNA replication in Saccharomyces cerevisiaeNature CommunicationsDecember 1, 2018Marta A. Garbacz, Scott A. Lujan, Adam B. Burkholder, Phillip B. Cox, Qiuqin Wu, Zhi-Xiong Zhou, James E. Haber, Thomas A. KunkelDNA purification with magnetic beads and the second strand synthesize using 4 U of T7 DNA polymerase (#M0274, New England Biolabs) and ARC76/77 duplex. After DNA purification with magnetic beads, unique indexes

(10) ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomesNucleic Acids ResearchMarch 16, 2018Hailong Wang et al.DNA polymerase I Klenow fragment (Kle; NEB, cat. no. M0210), T7 DNA polymerase (T7pol; NEB, cat. no. M0274) and λ exonuclease (λexo; NEB, cat. no. M0262): 25°C for 20 min, 75°C for 20 min, 50°C for 30 min, then

(11) Protein and Antibody Engineering by Phage DisplayMethods in EnzymologySeptember 3, 2016J.C. Frei, J.R. LaiHealthcare, Marlborough, MA, catalog #: GE27-9421-01)T7 DNA Polymerase (NEB, Ipswich, MA, catalog #: M0274L)T4 DNA Ligase (Invitrogen, Carlsbad, CA, catalog #: 15224-017)25 mM dNTPs (Fisher, Hampton, NH, catalog

(12) Transposon mapping using flanking sequence exponential anchored (FLEA) PCRProtocol ExchangeJuly 22, 2015Kentsis ResearchGroup, Anton Henssen, Julianne Carson, Alex KentsisMA USA) 5) Dynabeads kilobaseBINDER Kit \(#60101, Invitrogen, Waltham, MA, USA) 6) T7 DNA Polymerase \(#M0274S, New England BioLabs, Beverly, MA, USA) 7) Deoxyribose nucleotide triphosphates dNTP \ (#18427-088,

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