T4 RNA Ligase from Promega

Product Specs
ItemT4 RNA Ligase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM1051
Quantity500u
Concentration10u/μl

Specifications/Features

Unit Definition:
One unit catalyzes the formation of 1nmol of [5´-32P]rA14–20 into a phosphatase-resistant form in 30 minutes at 37°C in a total volume of 30μl. The reaction conditions are: 50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 5mM DTT, 1mM ATP and 10μM [5´-32P]rA14–20.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Multi-layered dosage compensation of the avian Z chromosome by increased transcriptional burst frequency and elevated translational ratesNat CommunOctober 13, 2025Natali Papanicolaou, Antonio Lentini, Sebastian Wettersten, Michael Hagemann-Jensen, Annika Krüger, Jilin Zhang, Christos Coucoravas, Ioannis Petrosian, Xian Xin, Ilhan Ceyhan, et al.(RA5, Illumina) by adding 1.2 μl RA5, 2 μl 10x T4 buffer and 2 μl T4 RNA ligase [Promega, cat. number: M1051] and incubating at 14 °C ON. RNA was precipitated and resuspended in 3 μl 10 mM Tris pH 7.5. Reverse

(2) Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposonsNat CommunJuly 2, 2025KeHuan K. Edmonds, Max E. Wilkinson, Daniel Strebinger, Hongyu Chen, Blake Lash, Clarissa C. Schaefer, Shiyou Zhu, Dangliang Liu, Shai Zilberzwige-Tal, Alim Ladha, et al.with synthetic 5’ phosphorylated oligonucleotide (molar ratio 1:25–100) using T4 RNA ligase (Promega, M1051) per manufacturer’s protocol and purified by RP-HPLC. For 5’ modification, in vitro transcribed RNA

(3) Chemical and topological design of multicapped mRNA and capped circular RNA to augment translationNature BiotechnologyJuly 1, 2025Hongyu Chen, Dangliang Liu, Abhishek Aditham, Jianting Guo, Jiahao Huang, Franklin Kostas, Kamal Maher, Mirco J. Friedrich, Ramnik J. Xavier, Feng Zhang, et al.ratio of 25:1 and diluted in 2× 50% PEG-8000, 10× T4 RNA ligase buffer, 10× T4 RNA ligase (Promega, M1051) and RNase-free water. The reaction was incubated at 37 °C for 0.5–1 h and inactivated by the addition

(4) Conserved heterodimeric GTPase Rbg1/Tma46 promotes efficient translation in eukaryotic cellsCell ReportsOctober 26, 2021Fuxing Zeng, Xin Li, Melissa Pires-Alves, Xin Chen, Christopher W. Hawk, Hong Jin18064022 Auxin,Sigma,Cat# I2886-25G TURBO DNase,Invitrogen,Cat# AM2238 T4 RNA ligase,Promega,Cat# M1051 n-dodecyl-D-maltoside (DDM),Macklin,CAS# 69227-93-6 Glutaraldehyde,Sagon,Cat# A500484-0250 Quantifoil

(5) Microarray and Degradome Sequencing Reveal MicroRNA Differential Expression Profiles and Their Targets in Pinellia pedatisectaPLoS ONESeptember 25, 2013Miao Dong, Dongfeng Yang, Qiulei Lang, Wei Zhou, Shaowei Xu, Tao Xu(5′-GUUCAGAGUUCUACAGUCCGACGAUCCAGCAG-3′) containing a 3′ Ecop 15I (NEB, R0646S) recognition site using T4 RNA ligase (Promega, M1051). Reverse transcription was carried out to generate first-strand cDNA using an oligo(dT) with a 3′-adapter

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T4 RNA Ligase

T4 RNA Ligase from Promega

Description

Specifications/Features

Promega

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T4 RNA Ligase from Promega

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