T4 RNA Ligase 2 (dsRNA Ligase) from New England Biolabs

Product Specs
ItemT4 RNA Ligase 2 (dsRNA Ligase)
CompanyNew England Biolabs
Price $86.00 Inquire
Catalog NumberM0239S
Quantity150 units
Concentration10,000 units/ml
ApplicationsFunctional Genomics
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to ligate 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µl in 30 minutes at 37°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Generation of precise and accurate engineered circRNAs using enzymatic ligationNucleic Acids ResMay 8, 2026Amrita Singh, Adela Dujsikova, Noah Mueller, Y Grace Chen2000 units of T4 DNA ligase (NEB #M0202M) were used for ligation at 37°C for 1 h. RNA ligase 2 (NEB #M0239L) reactions were either set up with DNA splint or RNA splint. Reaction conditions remained the same for

(2) RNA virus polymerase-helicase coupling enables rapid elongation through duplex RNACell ReportsApril 16, 2026Pim P.B. America, Subhas C. Bera, Arnab Das, Thomas K. Anderson, John C. Marecki, Flávia S. Papini, Jamie J. Arnold, Robert N. Kirchdoerfer, Craig E. Cameron, Kevin D. Raney, et al.technologies,RP8092H T4 Polynucleotide Kinase,New England Biolabs,Cat # M0201 T4 RNA ligase 2,New England Biolabs,Cat # M0239 Ulp1 peptidase,Byrd et al.39,N/A Phosphocellulose (P-11),"Whatman, Sigma-Aldrich",Cat # Z753807 Agarose,Sigma-Aldrich,Cat

(3) Evaluation of Nanopore direct RNA sequencing updates for modification detectionbioRxivApril 12, 2026Neda Ghohabi Esfahani, Andrew J Stein, Stuart Akeson, Talia Tzadikario, Miten Jainminute before being held at 4°C to facilitate annealing. For the ligation step, T4 RNA Ligase 2 (NEB #M0239) was used to ligate the adapter. The reaction mixture consisted of 5 μL of 10 μM of each construct (from

(4) tRNA isodecoder analysis using Nanopore ionic current signals and deep learningbioRxivJanuary 2, 2026Stuart Akeson, Pooria Daneshvar Kakhaki, Neda Ghohabi Esfahani, Julia L. Reinsch, Margaret L. Barry, Maddy Zamecnik, Talia Tzadikario, Robin L. Abu-Shumays, David M. Garcia, Kristin S. Koutmou, et al.#B6058S), 34 pmol of the custom adapter and 3 μl of T4 DNA ligase (2,000,000 units/mL, NEB Catalog #M0239S) in a total volume of 30ul. The protocol was followed from this point forward. Sequencing took place

(5) Spatiotemporal gene expression and cellular dynamics of the developing human heartNature GeneticsNovember 1, 2025Enikő Lázár, Raphaël Mauron, Žaneta Andrusivová, Julia Foyer, Mengxiao He, Ludvig Larsson, Nick Shakari, Sergio Marco Salas, Christophe Avenel, Sanem Sariyar, et al.(NEB, B0239SVIAL), 0.05 µM RCA primer, 1 U µl−1 RiboProtect (BLIRT, RT35), 1.0 U µl−1 T4 Rnl2 (NEB, M0239)) in a humidified chamber at 37 °C for 2 h. The sections were subsequently washed twice with PBS and

(6) ModiDeC: a multi-RNA modification classifier for direct nanopore sequencingNucleic Acids ResJuly 19, 2025Nicolò Alagna, Stefan Mündnich, Johannes Miedema, Stefan Pastore, Lioba Lehmann, Anna Wierczeiko, Johannes Friedrich, Lukas Walz, Marko Jörg, Tamer Butto, et al.the manufacturer’s instructions. Ligations were performed using T4 RNA ligase 2 (New England Biolabs, M0239), combining equimolar amounts of in vitro-transcribed RNA and the ligating oligo, 1× T4 RNA ligase buffer,

(7) Targeted Inhibition of Translation Initiation via 2’O-Methylation of Start CodonsbioRxivJune 19, 2025Emmely A. Patrasso, Hannah Serio, Adam Suh, Stephanie Mou, Kevin Vasquez, Siqi Wu, Yang Yi, Daniel Arangoannealing. Ligations were performed using 10 U of the T4 RNA ligase 2 (dsRNA ligase, New England Biolabs, Cat. #: M0239) for 4 hr at 37 °C according to the manufacturer’s suggestions. Reactions were stopped with 2 U of Turbo

(8) TRMT1L-catalyzed m22G27 on tyrosine tRNA is required for efficient mRNA translation and cell survival under oxidative stressCell ReportsJanuary 28, 2025Sseu-Pei Hwang, Han Liao, Katherine Barondeau, Xinyi Han, Cassandra Herbert, Hunter McConie, Amirtha Shekar, Dimitri G. Pestov, Patrick A. Limbach, Jeffrey T. Chang, et al.T4 polynucleotide kinase,NEB,M0201S T4 polynucleotide kinase reaction buffer,NEB,B0201S T4 RNA ligase,NEB,M0204S T4 RNA ligase 2,NEB,M0239S Leupeptin,Fisher,PI78435 [γ-32P]ATP,Perkin-Elmer,NEG502A250UC Nitrocellulose membrane,Bio-rad,1620115 anti-DYKDDDDK

(9) Genome-wide profiling of tRNA modifications by Induro-tRNAseq reveals coordinated changesNat CommunJanuary 26, 2025Yuko Nakano, Howard Gamper, Henri McGuigan, Sunita Maharjan, Jiatong Li, Zhiyi Sun, Erbay Yigit, Sebastian Grünberg, Keerthana Krishnan, Nan-Sheng Li, et al.of glycogen and the pellet was dissolved in 7.0 µL water. Ligation of 3´-RNA adapter T4 Rnl2 (NEB M0239L) was used for splint-mediated 3’-ligation of each tRNA library to a barcoded 35-mer RNA/DNA adapter

(10) 5′ terminal nucleotide determines the immunogenicity of IVT RNAsNucleic Acids ResearchJanuary 24, 2025Magdalena Wolczyk, Jacek Szymanski, Ivan Trus, Zara Naz, Tola Tame, Agnieszka Bolembach, Nila Roy Choudhury, Karolina Kasztelan, Juri Rappsilber, Andrzej Dziembowski, et al.produced 3′ donor RNA containing 5′ monophosphate essential for ligase activity using T4 Ligase 2 (NEB #M0239S). Additionally, the ssDNA fragment complementary to the 3′ end of the acceptor and the 5′ end of the

(11) Protocol for detection of tRNA-derived fragments in cells, tissues, and plasmaSTAR ProtocolsDecember 20, 2024Mengqian Yu, Pengyuan Liu, Yan LuTRI Reagent BD,Molecular Research Center,Cat#TB126-100 T4 RNA ligase 2 (T4 Rnl2),New England Biolabs,Cat#M0239L Annealing buffer for RNA oligos (5X),Beyotime,Cat#R0051 HiScript II 1st strand cDNA synthesis kit,Vazyme,Cat#R211 Premix Ex Taq (probe qPCR

(12) Selective RNA pseudouridinylation in situ by circular gRNAs in designer organellesNature CommunicationsOctober 24, 2024Lukas Schartel, Cosimo Jann, Anna Wierczeiko, Tamer Butto, Stefan Mündnich, Virginie Marchand, Yuri Motorin, Mark Helm, Susanne Gerber, Edward A. Lemke(D4060) following manufacturer instructions. Purified oligos were ligated using T4 RNA Ligase 2 by NEB (M0239), combining equimolar amounts of oligos to be ligated, 1xT4 RNA Ligase Buffer, 10% PEG8000, 10 U T4

(13) Spatial multimodal analysis of transcriptomes and metabolomes in tissuesNature BiotechnologyJuly 1, 2024Marco Vicari, Reza Mirzazadeh, Anna Nilsson, Reza Shariatgorji, Patrik Bjärterot, Ludvig Larsson, Hower Lee, Mats Nilsson, Julia Foyer, Markus Ekvall, et al.performed for 2 h at 37 °C with 1× T4 Rnl2 reaction buffer (NEB B0239SVIAL), 1 U µl−1 T4 Rnl2 (NEB, M0239), 1 U μl−1 RNase inhibitor (BLIRT, RT35) and rolling circle amplification (RCA) primer at a final concentration

(14) FUS reads histone H3K36me3 to regulate alternative polyadenylationNucleic Acids ResearchJune 10, 2024Junqi Jia, Haonan Fan, Xinyi Wan, Yuan Fang, Zhuoning Li, Yin Tang, Yanjun Zhang, Jun Huang, Dong FangT4 RNA ligase Reaction buffer (NEB, Cat. # B0216S), 8 μl 50% PEG8000, 1 μl T4 RNA ligase 2 (NEB, Cat. # M0239), 2 μl RNase inhibitor (NEB, Cat. # M0314S) and 4.2 μl H2O were added and incubated at room temperature

(15) Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutantsMolecular cellMay 16, 2024Yang C, Pataskar A, Feng X, Montenegro Navarro J, Paniagua I, Jacobs JJL, Zaal EA, Berkers CR, Bleijerveld OB, Agami R.peptides, and recombinant proteins" ,, T4 RNA Ligase 2 (dsRNA Ligase),New England biolabs,Catalog #M0239L "Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL",Thermo Fisher scientific,Catalog # 89883 BCA Protein Assay Kit

(16) Detecting m6A at single-molecular resolution via direct RNA sequencing and realistic training dataNature CommunicationsApril 18, 2024Adrian Chan, Isabel S. Naarmann-de Vries, Carolin P. M. Scheitl, Claudia Höbartner, Christoph Dieterichalong with 1.5 µl 10x T4 RNA ligase 2 buffer and 15 U of the T4 RNA ligase 2 (New England Biolabs, M0239S). Ligation reactions were carried out in a final reaction volume of 15 µl. The ligation reactions were

(17) RaptGen-Assisted Generation of an RNA/DNA Hybrid Aptamer against SARS-CoV-2 Spike ProteinBiochemistryApril 2, 2024Tatsuo Adachi, Shigetaka Nakamura, Akiya Michishita, Daiki Kawahara, Mizuki Yamamoto, Michiaki Hamada, Yoshikazu Nakamurathe presence of the reverse primer sequence (5′-ATATCTTTTGCTTATTCTCGAG-3′) by using T4 RNA ligase 2 (M0239, New England Biolabs), and reverse-transcribed by SuperScript IV (18090050, Thermo Fisher Scientific).

(18) Probing and perturbing riboswitch folding using a fluorescent base analoguePhotochemistry and PhotobiologyMarch 1, 2024Janson E. Hoeher, Natalie E. Sande, Julia R. Widomphosphate. Riboswitch samples were prepared by splinted ligation using T4 RNA ligase 2 (New England Biolabs M0239S). 5 nanomoles each of the 5’ and 3’ segments of the riboswitch and the DNA splint were combined and

(19) The layered costs and benefits of translational redundancyElifeMarch 2, 2023Parth K Raval, Wing Yui Ngan, Jenna Gallie, Deepa Agasheends of uncharged tRNAs using T4 RNA ligase 2 (dsRNA Ligase; New England BioLabs Inc, catalogue number M0239S). We reverse transcribed ligation products to cDNA using SuperScript III reverse transcriptase (Invitrogen;

(20) The layered costs and benefits of translational redundancyeLifeMarch 2, 2023Parth K Raval, Wing Yui Ngan, Jenna Gallie, Deepa Agasheends of uncharged tRNAs using T4 RNA ligase 2 (dsRNA Ligase; New England BioLabs Inc, catalogue number M0239S). We reverse transcribed ligation products to cDNA using SuperScript III reverse transcriptase (Invitrogen;

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