T4 DNA Polymerase from New England Biolabs

Product Specs
ItemT4 DNA Polymerase
CompanyNew England Biolabs
Price $276.00 Inquire
Catalog NumberM0203L
Concentration3 U/μl
Quantity750 units
ApplicationsDNA Manipulation
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is the amount of enzyme which incorporates 10 nmoles of dNTP into acid precipitable material in 30 minutes at 37°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Plant-associated fungi co-opt ancient antimicrobials for host manipulationSci AdvApril 29, 2026Fantin Mesny, Valentina Wolf, Ana López-Moral, Anton Kraege, Wilko Punt, Saifei Liu, Jinyi Zhu, Jiyeun Park, Yukiyo Sato, Bart P. H. J. ThommaNew England Biolabs, Ipswich, MA, USA; ref. B6002S), 0.5 μl of T4 DNA polymerase (New England Biolabs; ref. M0203S), and 5 μl nuclease-free water were added to the mixture, followed by an incubation at 12°C for 20 min.

(2) Aub, Vasa, and Armi concentration in phase-separated nuage is dispensable for piRNA biogenesis and transposon silencingRNAMarch 1, 2026Samantha Ho, Nicholas P. Rice, Tianxiong Yu, Zhiping Weng, William E. Theurkauf,Thermo Fisher Scientific,Cat# R0133 dNTP mix,NEB,Cat# N0447L DNA polymerase I,NEB,Cat# M0209S T4 DNA polymerase,NEB,Cat# M0203L Klenow DNA polymerase,NEB,Cat# M0210S T4 PNK,NEB,Cat# M0201L Klenow 3′ to 5′ exo,NEB,Cat# M0212L UDG,NEB,Cat#

(3) Rapid disassembly and Piwi-independent reassembly of Drosophila piRNA cluster heterochromatin following acute heat shockRNAMarch 1, 2026Nicholas P. Rice, Samantha Ho, Zhiping Weng, William E. Theurkauf,Thermo Fisher Scientific,Cat# R0133 dNTP mix,NEB,Cat# N0447L DNA polymerase I,NEB,Cat# M0209S T4 DNA polymerase,NEB,Cat# M0203L Klenow DNA polymerase,NEB,Cat# M0210S T4 PNK,NEB,Cat# M0201L Klenow 3′ to 5′ exo,NEB,Cat# M0212L UDG,NEB,Cat#

(4) Step-by-Step Protocol for In Situ Profiling of RNA Subcellular Localization Using TATA-seqBIO-PROTOCOLFebruary 20, 2026Xiao Jiang, Chu Xu, Lulu Hunumber: M0205) 19. RNase H (NEB, catalog number: M0297) 20. T4 DNA polymerase (NEB, catalog number: M0203) 21. Proteinase K (Thermo Fisher Scientific, catalog number: 25530049) 22. T7 High Yield RNA Transcription

(5) High-Resolution Chloroplast SNV Profiling of 409 Grapevine (Vitis vinifera L.) Cultivars Using Whole-Genome Shotgun SequencingInt J Mol SciFebruary 5, 2026Katarina Rudolf Pilih, Tomaž Kasunič, Tjaša Cesar, Denis Rusjan, Mitra Razi, Tatjana Jovanović-Cvetković, Aida Dervishi, Dragoslav Ivanišević, Katerina Biniari, Klime Beleski, et al.the sticky ends of the cut DNA were repaired by T4 DNA polymerase (NEB, Ipswich, MA, USA, cat. no. M0203), and barcoded adapters were ligated and nick repaired to each DNA accession using T4 DNA ligase (NEB,

(6) An integrated multi-omics and network analysis of neutrophil differentiation from initial- to late-stageGenome BiologyJanuary 30, 2026Qing Chen, Tianchang Yang, Yingping Hou, Kai Wang, Tingting Li, Longteng Wang, Cuiyun Dou, Panpan Cheng, Minglei Shi, Wei LiEssentially, a reaction mixture containing T4 Polynucleotide Kinase (NEB, M0201), T4 DNA Polymerase (NEB, M0203), and DNA Polymerase I (NEB, M0210) was used to simultaneously repair the ends of the sheared DNA and

(7) Neural stem cell quiescence is actively maintained by the epigenomeCell ReportsJanuary 27, 2026Anna Malkowska, Jan Ander, Andrea H. BrandmL),NEB,M0541L PEG-8000,Sigma-Aldrich,P5413 T4 DNA Ligase,NEB,M0202S T4 DNA polymerase (3 U/μL),NEB,M0203S T4 polynucleotide kinase,NEB,M0201S Quick ligase,NEB,M2200S 1MTris pH 8.0,–,– SpeedBead Magnetic

(8) Convergent evolution of scavenger cell development at brain bordersNatureJanuary 21, 2026Andrea U. Gaudi, Michelle Meier, Oguzhan F. Baltaci, Sayali Chowdhary, Frank J. Tulenko, Stefanie Dudczig, Sebastian-Alexander Stamatis, Scott Paterson, Hujun Yu, Maria Cristina Rondon Galeano, et al.previously described in refs. 62,63. gRNA was generated by annealing and elongation using T4 DNA polymerase (M0203S, New England Biolabs). A gene specific oligonucleotide sequence (Integrated DNA Technologies) was annealed

(9) Genome-wide regulon of NtrC reveals genetic regulation under nitrogen limitation in Methylomonas sp. DH-1iScienceJanuary 16, 2026Sukjae Han, Joon Young Park, Yong Hee Han, Ina Bang, Hyeonwoo Mun, Ok Kyung Lee, Ji Sung Hyung, Jeong-Geol Na, Eun Yeol Lee, Donghyuk Kim, et al.,New England Biolabs,Cat#M0264S phi29 DNA Polymerase,New England Biolabs,Cat#M0269S T4 DNA Polymerase,New England Biolabs,Cat#M0203S KAPA HiFi HotStart ReadyMix,Roche,Cat#KK2602 RNAprotect Bacteria Reagent,Qiagen,Cat#76506 Ribo-Zero

(10) The late arrival of domestic cats in China via the Silk Road after 3,500 years of human-leopard cat commensalismCell GenomicsJanuary 14, 2026Yu Han, Songmei Hu, Ke Liu, Xiao Xu, Ziyi Li, Sean Doherty, Alexandra Elizabeth Jamieson, Aurélie Manin, Sofia Granja Martins, Miaomiao Yang, et al.(10 U/L),NEB,Cat#M0201L T4 DNA Ligase (400 U/μL),NEB,Cat#M0202L T4 DNA Polymerase (3 U/μL),NEB,Cat#M0203L Bst 2.0 WarmStart DNA Polymerase (8 U/μL),New England Biolabs,Cat#M0538L Bovine Serum Albumin,Takara

(11) Bacterial chromatin remodeling associated with transcription-induced domains at pathogenicity IslandsNature CommunicationsJanuary 8, 2026Mounia Kortebi, Mickaël Bourge, Romain Le Bars, Erwin Van Dijk, Charles J. Dorman, Stéphanie Bury-Moné, Frédéric Boccard, Virginia S. Lioy(New England Biolabs, Cat# B0202S), 4 μL 10 mM dNTP mix, 5 μL T4 DNA polymerase (3 U/μL, New England Biolabs, Cat# M0203L), 5 μL T4 Polynucleotide Kinase (10 U/μL, Thermo Fisher Scientific, Cat# EK0032), 1 μL DNA polymerase I, large (Klenow) fragment

(12) Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tagBiotechnology & Biotechnological EquipmentDecember 31, 2025Enos C. Kline, Rose Duong, Qin Wang, Barry R. Lutz(NEB, N0446), 0.5 μL of 0.2 M Dithiothreitol, and 0.5 μL (1.4 units (u)) of T4 DNA polymerase (NEB, M0203) in a final volume of 20 μL. Reactions were incubated at 22 °C for 30 min followed by incubation at

(13) Molecular mechanism of a novel Aweak subtype in Chinese pedigrees: identification and analysis of a large genomic deletion in the promoter upstream distal region of the ABO geneBlood TransfusDecember 17, 2025Jiancheng Liu, Feng Shao, Jie Yang, Xiaoyin Mao, Xiaoyun Bu, Jing HaiT4 Polynucleotide Kinase (NEB, M0236 [New England Biolabs]), 0.5 μL (1.5U) T4 DNA Polymerase (NEB, M0203 [New England Biolabs]) and 1 μL (400U) T4 DNA Ligase (NEB, M0202 [New England Biolabs]), a total of

(14) Trans -regulation of heterochromatin underlies genetic variation in 3D genome contactsbioRxivDecember 16, 2025Haley J Fortin, Anna Z Struba, Christopher L Bakermix: 5 μL 10X NEB Buffer 2.1, 0.125 μL of dATP and dGTP at 10 mM each, 5 μL T4 DNA polymerase (NEB, M0203), and 39.8 μL distilled water. Samples were incubated at 20°C for 4 hours with interval shaking (1 min

(15) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Dempleenzymes was carried out at 80°C for 20 min. For the blunting step, nine units of T4 DNA polymerase (NEB, M0203S), 5 nmol of dNTPs (NEB, N0446S), and 1 μl of 10× NEB buffer r2.1 (NEB, B6002S) were added to the reaction

(16) Systematic Evaluation of GAPs and GEFs Identifies a Targetable Dependency for Hematopoietic MalignanciesCancer DiscoveryDecember 2, 2025Pu Zhang, Zhendong Cao, Xiangyu Pan, Yuqiao Liu, Cynthia Castro, Won Jun Kim, Takeshi Fujino, Jennifer Lewis, Jahan Rahman, Sanam Shahid, et al.R0739S "Rho Inhibitor, Rhosin - Calbiochem",EMD Millipore,Cat # 555460 T4 DNA polymerase,New England Biolabs,M0203L "Halt Protease & Phosphatase Inhibitor Cocktail, EDTA-free (100x)",Thermo Fisher Scientific,"Ref #78441,

(17) Ancient genomes reveal basal Asian ancestries and dynamic population interactions over time on the southern Tibetan PlateauiScienceNovember 21, 2025Jingkun Ran, Yichen Liu, Shargan Wangdue, Xiaoyan Yang, Tianyi Wang, Yu Gao, Peng Cao, Yan Tong, Qingyan Dai, Songtao Chen, et al.solution",NEB,cat#9804 T4 Polynucleotide Kinase (10 U/mL),NEB,cat#M0236L or S T4 DNA Polymerase (3 U/mL),NEB,cat#M0203L Quick Ligation Kit,NEB,cat#M2200L 2x HI-RPM hybridization buffer,Agilent,5118–5380 20% SDS,Serva,39575.01 SSC

(18) Identification of human RNA Polymerase II interactors at early stages of transcriptionbioRxivOctober 13, 2025Abdullah Ozer, Jin Joo Kang, Yuliang Tang, Kumar Yugandhar, Sun Yu, Michael D. DeBerardine, Karim Omar, John T. Lis, Haiyuan Yupresence of a 250 μM dNTP mix (each), 1x T4 DNA Ligase Buffer (NEB), 6 units T4 DNA Polymerase (NEB, Cat# M0203L), 20 units T4 PNK (NEB, Cat# M0201L), and 2.5 units Klenow Polymerase (NEB, Cat# M0210L) at RT for 30

(19) Gene-Pseudogene Inversions as a Hidden Source of Missing HeritabilitymedRxivOctober 7, 2025Ilaria Quartesan, Stefano Facchini, Arianna Manini, Ricardo Parolin Schnekenberg, Chiara Pisciotta, Stefania Magri, Sara Negri, Carlo Gaetano, Adriana Rebelo, Jacquelyn Schatzman Raposo, et al.(65602, Thermo Fisher Scientific), end-repaired and phosphorylated using T4 DNA polymerase and T4 PNK (M0203 and M0201L, New England BioLabs). Sequencing adaptors and indexes were added to DNA fragments using

(20) Efficient enrichment of synchronized mouse spermatocytes suitable for genome-wide analysis.Methods in Molecular BiologySeptember 27, 2025Agustin Carbajal, Irma Gryniuk, Rodrigo O. de Castro, Roberto J. PezzaKK4854). End-repair mix: (0.66 mM dNTP mix (NEB, cat. # N0447S), 100 U/ml T4 DNA polymerase (NEB, cat. # M0203L), 33 U/ml Klenow fragment (NEB, cat. # M0210S), 333 U/ml T4 PNK (NEB, cat. # M0201L), and 1.67 × T4

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