SwaI from New England Biolabs

Product Specs
ItemSwaI
CompanyNew England Biolabs
Price $308.00 Inquire
Catalog NumberR0604L
Quantity10000 units
Concentration10 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

ATTT/AAAT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Development of a novel alpha7-nicotinic acetylcholine receptor-selective cell-penetrating peptide for intracellular cargo transportDrug DeliveryDecember 31, 2025Lahra Weber, Brittany C. V. O’Brien, Maegan M. Weltzinethidium bromide. Before cRNA synthesis, the cDNAs were linearized using restriction enzymes (SwaI (cat. # R0604) for pCI and NheI (cat. # R3131) for pSHE/pSGEM constructs). The linearized DNA was then treated with

(2) Purification and characterization of recombinant human translation initiation factor eIF3Protein SciDecember 23, 2025Irene Diaz-Lopez, Yuliya Gordiyenko, Philipp K. Zuber, Xueyan Li, V. Ramakrishnan10 μg of pBIG1 plasmids (A, B, C, and D) were linearized with 20 U of SwaI restriction enzyme (NEB, R0604L) for 2 h at 25°C. Correct linearization was checked on an analytical agarose gel and the linearized

(3) Identification and Phylogenetic Characterisation of Novel Adeno‐Associated Virus Capsids in Non‐Human Primate TissuesCell ProliferationSeptember 16, 2025Liyu Zhu, Kai Xu, Yali Ding, Kailun Liu, Jing Liu, Zongren Hou, Rui Niu, Ning Yang, Hualing Qin, Baoyang Hu, et al.plasmid, 112862), which had been linearised by sequential digestion with SwaI (New England Biolabs, R0604S) and EcoRV‐HF (New England Biolabs, R3195V), using the seamless cloning kit (CloneSmarter, C5891‐25).

(4) Alzheimer-mutant γ-secretase complexes stall amyloid β-peptide productioneLifeFebruary 11, 2025Arafi P, Devkota S, Williams E, Maesako M, Wolfe MS.,New England Biolabs,Cat. No. R0547S, "Chemical compound, drug",SwaI restriction enzyme,New England Biolabs,Cat. No. R0604S, "Chemical compound, drug",T4 DNA Polymerase,New England Biolabs,Cat. No. M0203L, "Chemical compound,

(5) Virus-induced vesicular acidification enhances HIV immune evasionbioRxivDecember 21, 2024Marianne E. Yaple-Maresh, Giselle G. Flores, Gretchen E. Zimmerman, Francisco Gomez-Rivera, Kathleen L. Collins,New England BioLabs,Cat. R3189S XhoI Enzyme,New England BioLabs,Cat. R0146 SwaI Enzyme,New England BioLabs,Cat. R0604S XbaI Enzyme,New England BioLabs,Cat. R0145 MluI Enzyme,New England BioLabs,Cat. R3198 BssHII Enzyme,New England BioLabs

(6) Alzheimer-mutant γ-secretase complexes stall amyloid β-peptide productionbioRxivNovember 27, 2024Parnian Arafi, Sujan Devkota, Emily Williams, Masato Maesako, Michael S. Wolferestriction enzyme,New England Biolabs,Cat. No. R0547S SwaI restriction enzyme,New England Biolabs,Cat. No. R0604S T4 DNA Polymerase,New England Biolabs,Cat. No. M0203L "DOPC (1,2-dioleoyl-sn-glycerol-3-phosphocholine)",Avanti

(7) A novel N 4, N 4-dimethylcytidine in the archaeal ribosome enhances hyperthermophilyProceedings of the National Academy of SciencesNovember 5, 2024Kristin A. Fluke, Nan Dai, Eric J. Wolf, Ryan T. Fuchs, P. Shing Ho, Victoria Talbott, Liam Elkins, Yueh-Lin Tsai, Jackson Schiltz, Hallie P. Febvre, et al.bp terminal extensions with homology to pTS700 at SwaI. pTS700 was linearized with SwaI (NEB, cat# R0604S) before the insert was cloned into pTS700 by ligation independent cloning. The coding sequence of the

(8) Combining CRISPR-Cas-mediated terminal resolution with a novel genetic workflow to achieve high-diversity adenoviral librariesMolecular Therapy - Methods & Clinical DevelopmentJune 13, 2024Julian Fischer, Ariana Fedotova, Lena Jaki, Erwan Sallard, Anja Erhardt, Jonas Fuchs, Zsolt Ruzsicsintermediate bacmids pBWH-C5-pIX-Kan or pBWH-fC5-E1Kn were digested by SwaI (New England Biolabs, #R0604S) overnight and heat-inactivated at 65°C, after which these preparations were used as vectors in the

(9) The human alpha7 nicotinic acetylcholine receptor is a host target for the rabies virus glycoproteinFrontiers in Cellular and Infection MicrobiologyMay 21, 2024Brittany C. V. O’Brien, Shelly Thao, Lahra Weber, Helen L. Danielson, Agatha D. Boldt, Karsten Hueffer, Maegan M. Weltzinfor cRNA synthesis, cDNAs were linearized using restriction enzymes (New England Biolabs) SwaI (Cat# R0604S) or NheI-HF (Cat# R3131S). Following cDNA linearization, samples were treated with proteinase K (30min

(10) Detection of ribonucleotides embedded in DNA by Nanopore sequencingCommunications BiologyApril 23, 2024Lavinia Grasso, Adriano Fonzino, Caterina Manzari, Tommaso Leonardi, Ernesto Picardi, Carmela Gissi, Federico Lazzaro, Graziano Pesole, Marco Muzi-Falconiat 25 °C with MscI (New England Biolabs catalogue # R0534) and SwaI (New England Biolabs catalogue # R0604) restriction enzymes in NEB buffer 3.1. Digestion products were run on a 0.8% agarose gel, and only

(11) Development of CNS tropic AAV1-like variants with reduced liver-targeting following systemic administration in miceMolecular TherapyMarch 6, 2024Matthieu Drouyer, Jessica Merjane, Deborah Nazareth, Maddison Knight, Suzanne Scott, Sophia H.Y. Liao, Samantha L. Ginn, Erhua Zhu, Ian E. Alexander, Leszek Lisowskiovernight alongside the pFT-spleen focus-forming visrus (SFFV) backbone with SwaI (NEB, catalog no. R0604S) and NsiI (NEB, catalog no. R0127S). Following separation on 1% (w/v) agarose gel and purification using

(12) Familial Alzheimer mutations stabilize synaptotoxic γ-secretase-substrate complexesCell reportsFebruary 27, 2024Devkota S, Zhou R, Nagarajan V, Maesako M, Do H, Noorani A, Overmeyer C, Bhattarai S, Douglas JT, Saraf A, et al.restriction enzyme,New England Biolabs,Cat. No. R0547S Swa1 restriction enzyme,New England Biolabs,Cat. No. R0604S BamH1 restriction enzyme,New England Biolabs,Cat. No. R01365 NgoM4 restriction enzyme,New England

(13) The YBX3 RNA-binding protein post-transcriptionally controls SLC1A5 mRNA in proliferating and differentiating skeletal muscle cellsJournal of Biological ChemistryFebruary 1, 2024Silina Awad, William Skipper, William Vostrejs, Kendall Ozorowski, Kristen Min, Liva Pfuhler, Darshan Mehta, Amy CookeWisconsin-Madison) was digested with restriction enzymes, NotI-HF (NEB cat# R3189S) and SwaI (NEB cat# R0604S), following protocols as described by NEBCloner. All inserts were amplified from C2C12 cDNA. Primers

(14) Genome sequence and characterization of a novel Pseudomonas putida phage, MiCathSci RepDecember 9, 2023James Jaryenneh, Joseph S. Schoeniger, Catherine M. Mageeneyno. R3195S), HaeIII (NEB, catalog no. R0108T), NdeI (NEB, catalog no. R0111S), SwaI (NEB, catalog no. R0604S), RsaI (NEB, catalog no. R0167S). Restriction enzymes digestions were prepared by combining 10 × NEB

(15) Insights from the Construction of Adenovirus-Based Vaccine Candidates against SARS-CoV-2: Expecting the UnexpectedVirusesOctober 25, 2023Denice Weklak, Julian Tisborn, Maurin Helen Mangold, Raphael Scheu, Harald Wodrich, Claudia Hagedorn, Franziska Jönsson, Florian Kreppelbacterial bacmid backbone by restriction digestion with SwaI (New England Biolabs GmbH, Ipswich, MA, USA, R0604S). In short, 2.5 µg of DNA was incubated with 3 U SwaI in supplied buffer at 25 °C overnight. HEK293 cells

(16) Multiplexed long-read plasmid validation and analysis using OnRampGenome ResearchMay 8, 2023Camille Mumm, Melissa L. Drexel, Torrin L. McDonald, Adam G. Diehl, Jessica A. Switzenberg, Alan P. BoyleB7203S), was added to 1×, and the final volume was adjusted with nuclease-free water to 200 µL. SwaI (NEB R0604L) was added according to the total amount of DNA present for linearization (minimum, 10 units enzyme

(17) IFNγ is a central node of cancer immune equilibriumCell reportsMarch 28, 2023Walsh MJ, Stump CT, Kureshi R, Lenehan P, Ali LR, Dougan M, Knipe DM, Dougan SK.IFNγ,Peprotech,315-05 Recombinant Human IL-2,Peprotech,200-02 XhoI,NEB,R0146S NotI,NEB,R0189S BbsI,NEB,R0539S SwaI,NEB,R0604S BsmBI-v2,NEB,R0739S T4 DNA ligase,NEB,M0202S Puromycin Dihydrochloride,ThermoFisher,A1113803 Hygromycin B (50 mg/mL

(18) Easy efficient HDR-based targeted knock-in in Saccharomyces cerevisiae genome using CRISPR-Cas9 systemBioengineeredJanuary 5, 2023Rajveer Singh, Shivani Chandel, Arijit Ghosh, Anupam Gautam, Daniel H. Huson, V. Ravichandiran, Dipanjan Ghosh(Himedia-GRM026), LB broth (BD-240230), pML107 (Addgene plasmid # 67639), BclI (NEB-R0160S), SwaI (NEB-R0604S), EcoRI (NEB-R0101S), Ampicillin (SRL-69-52-3), QIAprep Spin Miniprep Kit (Qiagen-27,104), QIAquick

(19) Artificial Self-assembling Nanocompartment for Organizing Metabolic Pathways in YeastACS Synthetic BiologyDecember 17, 2021Li Chen Cheah, Terra Stark, Lachlan S. R. Adamson, Rufika S. Abidin, Yu Heng Lau, Frank Sainsbury, Claudia E. Vickerscontains a K. lactis LEU2 marker. For all constructs, the plasmids were first digested with SwaI (NEB #R0604) and transformed using the LiAc/SS carrier DNA/PEG method,53 leading to stable single-copy integration

(20) Nitric oxide controls proliferation of Leishmania major by inhibiting the recruitment of permissive host cellsImmunityDecember 14, 2021Formaglio P, Alabdullah M, Siokis A, Handschuh J, Sauerland I, Fu Y, Krone A, Gintschel P, Stettin J, Heyde S, et al.R0142 BglII,New England Biolabs,Cat# R0144 BfrI,New England Biolabs,Cat# R0520 SwaI,New England Biolabs,Cat# R0604 Hygromycin B,ThermoFisher,Cat# 10687010; CAS Number 31282-04-9 Calf intestinal alkaline phosphatase,New

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SwaI

SwaI from New England Biolabs

Sequence

New England Biolabs

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SwaI from New England Biolabs

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