Biotinylated Protein Ladder Detection Pack from Cell Signaling Technology

Product Specs
ItemBiotinylated Protein Ladder Detection Pack
CompanyCell Signaling Technology
Price $311.00 Inquire
Catalog Number7727L
Quantity3250 µl
Conjugate/Tag/LabelBiotin
Research AreaRelated Products
Nucleic Acid TypeProtein Ladder
Features (standardized)Unstained, Tagged
Size Range9 - 200 kDa
TypeProtein Ladder

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) A synthetic benzoxazine dimer derivative targets c‐Myc to inhibit colorectal cancer progressionMolecular OncologyMarch 1, 2026Nicharat Sriratanasak, Bodee Nutho, Worawat Wattanathana, Narumon Phaonakrop, Bunnatut Panasawatwong, Katharina Erlenbach‐Wuensch, Sittiruk Roytrakul, Regine Schneider‐Stock, Pithi Chanvorachote(#2946), p‐Ser62‐c‐Myc (#13748), p‐Thr58‐c‐Myc (#46650), as well as a biotinylated protein ladder (#7727) and an anti‐biotin, HRP‐linked antibody (#7075), were acquired from Cell Signalling Technology (Danvers,

(2) Novel Active Homo-Aza (Lactam) Steroidal Antimetabolites for the Treatment of Human Pancreatic and Colorectal CancerPharmaceuticals (Basel)February 17, 2026Konstantinos E. Alifieris, Panagiotis Dalezis, Sofia Sagredou, Ioanna A. Anastasiou, Maria Deligiorgi, Christos Siokatas, Nikolaos Spanakis, Konstantinos Almpanakis, Maria Voura, Kyriakos Orfanakos, et al.determined according to the Bradford assay. All the antibodies (Table 12), biotinylated protein ladder (#7727, dilution 1:1000), nitrocellulose membranes (#12369), and 20× lumiGLO reagent and 20× peroxide (#95538S),

(3) Differential regulation of expression of the protein kinases DYRK1A and DYRK1B in cancer cellsScientific ReportsOctober 13, 2024Vincent Andreas Vorwerk, Gerrit Wilms, Aaron Babendreyer, Walter Beckerpolyacrylamide gel electrophoresis analysis and Western blotting. The Biotinylated Protein Ladder Detection Pack (CST #7727) was used as size standard. Membranes were blocked in TBST supplemented with 5% BSA overnight at 4 °C

(4) NADPH oxidase 1: A target in the capacity of dimeric ECG and EGCG procyanidins to inhibit colorectal cancer cell invasionRedox BiologySeptember 1, 2023Wei Zhu, Patricia I. OteizaHRP-conjugated (#7074), anti-rabbit biotinylated (#14708), streptavidin (#3999) and the biotinylated ladder (#7727) were from Cell Signaling Technology, Inc. (Danvers, MA). The primary antibody for NOX1 (#ab78016) was

(5) Targeting Phospholipase D Pharmacologically Prevents Phagocytic Function Loss of Retinal Pigment Epithelium Cells Exposed to High Glucose LevelsInternational journal of molecular sciencesOctober 5, 2022Bermúdez V, Tenconi PE, Echevarría MS, Asatrian A, Calandria JM, Giusto NM, Bazan NG, Mateos MV.Precision Plus Protein Dual Color Standards (#1610374. Bio-Rad Life Science group) and Biotinylated Protein Ladder Detection Pack (#7727, Cell Signaling Technology, Inc) in the case of ABC cell Western Blots. 4.8. Real-Time Quantitative PCR (qPCR

(6) Biallelic loss-of-function variants in WDR11 are associated with microcephaly and intellectual disabilityEuropean journal of human genetics : EJHGNovember 1, 2021Haag N, Tan EC, Begemann M, Buschmann L, Kraft F, Holschbach P, Lai AHM, Brett M, Mochida GH, DiTroia S, et al.were used. PageRuler Prestained (ThermoScientific) and biotinylated Protein Ladders (Cell Signaling, 7727S) were used for protein molecular weight estimation. Detection was done using Clarity Western ECL Substrate

(7) A Role for TGFβ Signaling in Preclinical Osteolytic Estrogen Receptor-Positive Breast Cancer Bone Metastases ProgressionInternational journal of molecular sciencesApril 24, 2021Cheng JN, Frye JB, Whitman SA, Kunihiro AG, Pandey R, Funk JL.Danvers, MA, USA), or Biotinylated Protein Ladder followed by anti-biotin HRP-linked secondary antibody (#7727, CST, Danvers, MA, USA), were used to estimate probed-proteins ≤190 kDa in molecular weight, and Precision

(8) Evaluation of [Cys(ATTO 488)8]Dermorphin-NH2 as a novel tool for the study of μ-opioid peptide receptorsPLOS ONEApril 23, 2021Despina Giakomidi, Mark F. Bird, John McDonald, Erika Marzola, Remo Guerrini, Serena Chanoch, Nidhuna Sabu, Barbara Horley, Girolamo Calo, David G. Lambertheated to 95˚C for 5min. 50μl of each sample, along with protein marker (biotinylated protein ladder #7727 Cell signalling technology) was loaded onto an 10% SDS-PAGE gel. Proteins were separated by electrophoresis

(9) Hexameric procyanidins inhibit colorectal cancer cell growth through both redox and non-redox regulation of the epidermal growth factor signaling pathwayRedox biologyJanuary 1, 2021Daveri E, Adamo AM, Alfine E, Zhu W, Oteiza PI.secondary antibodies anti-rabbit HRP-conjugated (#7074) or biotinylated (#14708) and biotinylated ladder (#7727) were from Cell Signaling Technology, Inc. (Danvers, MA, USA). Primary antibodies against α-tubulin

(10) SON DNA-binding protein mediates macrophage autophagy and responses to intracellular infectionFEBS lettersSeptember 1, 2020Gregory DJ, DeLoid GM, Salmon SL, Metzger DW, Kramnik I, Kobzik L.gel (Invitrogen) with Biotinylated Molecular Weight Marker (Cell Signalling Technologies catalogue 7727). See Table 2 for antibodies. Images were acquired on an HD2 gel imager (Alpha Innotech) with ALTA U

(11) Multiple novel hepatocellular carcinoma signature genes are commonly controlled by the master pluripotency factor OCT4Cellular oncology (Dordrecht)April 1, 2020Ye C, Zhang X, Chen X, Cao Q, Zhang X, Zhou Y, Li W, Hong L, Xie H, Liu X, et al.secondary antibody (7074), normal Rabbit IgG (2729) and a biotinylated protein ladder detection pack (7727) were all purchased from Cell Signaling Technology. An anti-GAPDH (AG019) antibody was purchased from

(12) Combating viral contaminants in CHO cells by engineering innate immunityScientific ReportsDecember 1, 2019Austin W. T. Chiang et al.(Thermo Fisher Scientific) under reducing conditions. As a molecular weight marker, protein ladder (cat# 7727S) from Cell Signaling Technology (Danvers, MA) was used. Nitrocellulose membranes and iBlot™ transfer

(13) Transcriptomic changes triggered by ouabain in rat cerebellum granule cells: Role of α3- and α1-Na+,K+-ATPase-mediated signalingPLOS ONESeptember 26, 2019Larisa V. Smolyaninova, Alexandra A. Shiyan, Leonid V. Kapilevich, Alexander V. Lopachev, Tatiana N. Fedorova, Tatiana S. Klementieva, Aleksey A. Moskovtsev, Aslan A. Kubatiev, Sergei N. Orlovmembrane. Biotinylated proteins served as molecular weight markers (Cell Signaling Technologies, Cat#7727). The membranes were blocked and incubated overnight with primary and appropriate secondary HRP-conjugated

(14) Transcription Factor IRF4 Dysfunction Affects the Immunosuppressive Function of Treg Cells in Patients with Primary Immune ThrombocytopeniaBioMed research internationalJanuary 1, 2019Tang M, Cheng L, Li F, Wu B, Chen P, Zhan Y, Hua F, Min Z, Ke Y, Liu C, et al.and combined with biotin labeled protein molecular weight standard (Cell Signaling Technology, Cat# 7727) 10 𝜇l per lane. After electrophoresis of protein power transferred to nitrocellulose membrane (Cell Signaling Technology

(15) Exceptionally high-affinity Ras binders that remodel its effector domainJournal of Biological ChemistryMarch 1, 2018John H. McGee, So Youn Shim, Seung-Joo Lee, Paige K. Swanson, Sam Y. Jiang, Michael A. Durney, Gregory L. VerdineHealth) using a biotinylated ladder and anti-biotin-HRP (Cell Signaling Technology, catalog numbers 7727 and 7075). Nuclear magnetic resonance To prepare 15N-labeled KRas or miniprotein, cells were grown

(16) Loss of inversin decreases transepithelial sodium transport in murine renal cellsAmerican journal of physiology. Cell physiologyDecember 1, 2017Kulkarni NH, Smith RC, Blazer-Yost BL.analysis [anti-rabbit IgG conjugated to horseradish peroxidase (HRP) no. 7074S] and biotinylated protein ladder (no. 7727) were from Cell Signaling Technology (Beverly, MA). Cell culture. The mouse cortical collecting duct

(17) Proteomic analysis of affinity-purified extracellular proteasomes reveals exclusively 20S complexesOncotargetNovember 24, 2017Valentina A. Kulichkova, Tatiana O. Artamonova, Olga G. Lyublinskaya, Mikhail A. Khodorkovskii, Alexey N. Tomilin, Anna S. TsimokhaBio-Rad Laboratories) and analyzed by Western blotting, using antibodies against biotin (HRP-conjugate, #7727, 1:2500, Cell Signaling Technology) and primary antibodies to α-tubulin mouse (T6074, 1:5000), β-tubulin

(18) Novel GM-CSF signals via IFN-γR/IRF-1 and AKT/mTOR license monocytes for suppressor functionBlood advancesJune 13, 2017Ribechini E, Hutchinson JA, Hergovits S, Heuer M, Lucas J, Schleicher U, Jordán Garrote AL, Potter SJ, Riquelme P, Brackmann H, et al.peroxidase (HRP) (#7074), P-4E-BP1 (#2855P), p-mTOR (#5536P), anti-mouse-HRP (#7076), and anti-biotin-HRP (#7727) (all Cell Signaling), Rel A p65 (clone F-6, Santa Cruz), NF-kB p100 (polyclonal rabbit, Cell Signaling),

(19) Activation of transient receptor potential vanilloid 4 involves in hypoxia/reoxygenation injury in cardiomyocytesCell death & diseaseMay 25, 2017Wu QF, Qian C, Zhao N, Dong Q, Li J, Wang BB, Chen L, Yu L, Han B, Du YM, et al.electrophoresis gels, and 10 μl biotinylated protein ladder (Cell Signaling Technology, Danvers, MA, USA, #7727) loaded into a separate lane. Then, it was transferred to a nitrocellulose membrane by Electrophoresis

(20) Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - β1 (TGF-β1) and potential effects on migration and invasionBMC cancerMay 19, 2017Magnussen SN, Hadler-Olsen E, Costea DE, Berg E, Jacobsen CC, Mortensen B, Salo T, Martinez-Zubiaurre I, Winberg JO, Uhlin-Hansen L, et al.The PNGase F kit (P0704S) was from New England BioLabs (Beverly, MA). Biotinylated protein ladder (7727, lot: 21) was from Cell Signaling Technology (Danvers, MA). Western blotting Luminol Reagent (sc2048)

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