High Sensitivity D5000 ScreenTape Assay for TapeStation Systems from Agilent Technologies

Product Specs
ItemHigh Sensitivity D5000 ScreenTape Assay for TapeStation Systems
CompanyAgilent Technologies
Price Supplier Page View Company Product Page
Catalog Number5067-5592
Time15 samples < 20 min
96 samples < 120 min
TypeHigh Sensitivity D5000 Assay
Range100 to 5000 bp

Features

Uses per ScreenTape: 15
Sensitivity: 5 pg/µL
Sizing Accuracy: ± 15 %

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Agilent Technologies

5301 Stevens Creek Blvd

Santa Clara, California 95051

United States

Phone: +1 877-424-4536

Company Profile

Website: www.agilent.com

(1) Protocol to maximize single-nuclei multiome library complexity for limited primary tissues via optimized sample preparation and library synthesisSTAR ProtocolsSeptember 1, 2026Faizan Uddin, Awadhesh Pandit, Akshay Kudpaje, Dimple Notani, Radhakrishnan Sabarinathan,Miltenyi Biotec,130-096-334 gentleMACS®™ Dissociator,Miltenyi Biotec,130-093-235 High Sensitivity D5000 ScreenTape,Agilent Technologies,5067-5592 "Macro Pipette Tips, 5ml",Tarsons,521031 MACS® MultiStand,Miltenyi Biotec,130-042-303 MACS® SmartStrainers

(2) Type V Collagen Controls Decidual Extracellular Matrix Organization and Angiogenesis During Embryo ImplantationbioRxivJuly 25, 2026Mona Gebril, Emily Kinneston, Rimi Das, Joseph Boyd, John P Lydon, Shanmugasundaram NallasamyCat. No. G2991BA) with High Sensitivity D5000 ScreenTape and associated reagents (Agilent, Cat. No. 5067–5592). Libraries were sequenced at the Hubbard Center for Genome Studies (University of New Hampshire) on

(3) Comparative single-nucleus transcriptomics reveals asymmetric evolution of the Drosophila male and female germlinesPLoS BiolJuly 20, 2026Imtiyaz E. Hariyani, Sudeshna Das, Emma M. Le, Carmen Gamero-Castano, Tina Soroudi, Joshua Choi, Spring Momeni, Justin C. Kim, Rongying Lu, Vivek Swarup, et al.#Q32851). The average fragment length of each sub-library was measured using the D5000 HS kit (Agilent, #5067-5592, #5067-5593). Finally, the eight sub-libraries were sequenced on the Illumina Novaseq 6000 S4 platform

(4) Arrayed single-gene perturbations identify drivers of human anterior neural tube closureElifeJuly 7, 2026Roya E Huang, Giridhar M Anand, Heitor C Megale, Jason Chen, Chudi Abraham-Igwe, Sharad Ramanathan(4200 TapeStation instrument, Agilent Technologies) using High Sensitivity D5000 tape and reagents (Part Nos. 5067-5592 and 5067-5593). The amplified cDNA from poly-adenylated mRNA was fragmented, end repaired, and A-tailed

(5) Macrophage-specific targeting of histone demethylases with small-molecule inhibitors suppresses inflammatory response in vivoJ Biol ChemJuly 6, 2026Niranjana Natarajan, Thaybah Ahmed, Balaji Govindaswamy, Devika S. Manickam, Jishnu Das, Kabirul Islam, Partha Duttaassessed for quality on an Agilent TapeStation 2200 using the D5000 High sensitivity ScreenTape (Agilent, #5067–5592). All samples passed QC with full length cDNA were advanced to library preparation using Illumina Nextera

(6) LOTR-Seq: A protocol for large-scale simultaneous single-cell long-read genotyping of transcriptsSTAR ProtocJuly 1, 2026Julian Grabek, Leanne Cooper, Rohit Haldar, Jenny Quiatchon, Nadine Fitzpatrick, Paul Collins, Steven W. Lane, Megan J. Bywater, Jasmin StraubeGenomics,10X-1000120 Dual Index Kit TT Set A,10x Genomics,10X-1000215 High Sensitivity D5000 ScreenTape,Agilent,5067–5592 High Sensitivity D5000 Reagents,Agilent,5067–5593 High Sensitivity D5000 Ladder,Agilent,5067–5594 Qubit dsDNA HS Assay Kit

(7) Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disordersCellJuly 1, 2026Zinan Zinan Zhou, Lovelace J. Luquette, Guanlan Dong, Junho Kim, Jayoung Ku, Kisong Kim, Nandini Ramesh, Mingyun Bae, Ann Caplin, Diane D. Shao, et al.High Sensitivity D5000 Reagents,Agilent Technologies,5067-5593 High Sensitivity D5000 ScreenTape,Agilent Technologies,5067-5592 Genomic DNA ScreenTape,Agilent Technologies,5067-5365 Genomic DNA Reagents,Agilent Technologies,5067-5366 High

(8) Cannabidiol Activates Integrated Stress Response Signaling and Immune Trafficking Programs in an A375 Melanoma–Jurkat T Cell Coculture Model: A Multi-Omics AnalysisACS OmegaJune 30, 2026Ni Deng, Huifang Li, Ang Cai, Christopher L. Hemme, Jonghae Youn, Ryan Janis, Matthew D. Stone, Hang Ma, Chang LiuG2991Ba, Santa Clara, CA, USA) with a High Sensitivity D5000 ScreenTape and associated reagents (Agilent, 5067–5592(3,4)). For RNA-seq, samples were sequenced at the Hubbard Center for Genome Studies (University of

(9) Skin-derived G-CSF activates pathological granulopoiesis upon psoriasisEMBO Molecular MedicineJune 16, 2026Tomson Kosasih, Tatsuya Morishima, Sohyeon Lee, Jungyeon Yoon, Kanako Wakahashi, Pilhan Kim, Aiko Sada, Hitoshi TakizawaFC-131-1096 Index primers,Illumina,Cat# FC-131-2001 High Sensitivity D5000 Screen Tape,Agilent,Cat# 5067-5592 GenNext® NGS Library Quantification kit,Toyobo,Cat# NLQ-101 Fixation/Permeabilization Concentrate,eBioscience,Cat#

(10) The critical role of the endogenous immune compartment after CAR T cell therapy in recurrent GBMCellJune 15, 2026Nelson F. Freeburg, Daniel Chafamo, Gayathri Konanur Konanur Gopikrishna, Regan M. Murphy, Jacqueline J. Peng, Shridhar Parthasarathy, Sydney Dumont, Edward G. Estrada, Meghan T. Logun, Yusha Sun, et al.control was performed using the Agilent Technologies Tapestation and High-Sensitivity D5000 Screentape (5067–5592). Illumina sequencing of sc/snRNA-seq Sequencing of single-cell CSF and infusion product and single-nucleus

(11) Transcriptomic and lipidomic analysis of the cryptococcal lung granuloma reveals macrophage boundary programs and sphingolipid remodelingNature CommunicationsMay 28, 2026Veronica Soares Brauer, Kathryn Takemura, Barbara Rosati, Marinaldo Pacífico Cavalcanti-Neto, Deveney Dasilva, Neelu Singh, Tyler Normile, Gabriella Vaccaro, Giuseppe Caso, Harpreet Singh, et al.cDNA was performed on a Tapestation 4200 (Agilent) using D5000 High Sensitivity ScreenTapes (Agilent, 5067-5592). 5’ single nuclei RNA-seq libraries were generated using 50 ng of the amplified cDNA. After fragmentation,

(12) Protocol for isolating cells from a single cryopreserved gastrointestinal endoscopic biopsy for single-cell RNA sequencingSTAR ProtocMay 21, 2026Daniel Horowitz, Joshua Rusbuldt, Lynn Tomsho, Tae Lee, Klebea Sohn, Arun Kannan, Darren RuaneGenomics,1000268 Next GEM Chip G,10X Genomics,2000177 High Sensitivity D5000 ScreenTape,Agilent Technologies,5067-5592 High Sensitivity D1000 ScreenTape,Agilent Technologies,5067-5584 Dual Index Kit TT Set A,10X Genomics,1000215 PhiX

(13) Low-Strength Type I Interferon Signaling Promotes CAR T Cell Treatment EfficacyCancer Immunol ResMay 11, 2026Erting Tang, Yifei Hu, Guoshuai Cao, Duy-Thuc Nguyen, Nicholas W. Asby, Nada S. Aboelella, Hanna A. Ruiz, Xiaolei Cai, Wenbo Zhang, Yu Zhao, et al.(Invitrogen, Q32851), quality-checked for fragment sizes via high-sensitivity D5000 screentapes (Agilent, 5067–5592), pooled, and sequenced (Illumina, Novaseq-6000, RRID:SCR_016387 and Nextseq-550, RRID:SCR_016381). Single-cell RNA-seq

(14) A Head and Neck Cancer Patient-Specific Microphysiological System for Predicting Response to ChemoradiationbioRxivMay 5, 2026Adeel Ahmed, Nathan Hendrikse, Rene Welch Schwartz, Yuanshan Li, Marcos Lares, Cassidy K. Felix, Adam R. Burr, Irene M. Ong, Paul M. Harari, David J. Beebe, et al.barcoded cDNA libraries. Libraries were assessed using High Sensitivity D5000 ScreenTape (Agilent, PN-5067-5592) with High Sensitivity D5000 Reagents (Agilent, 5067-5593) on a TapeStation and sequenced on a NovaSeq X Plus

(15) Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing dataNucleic Acids ResApril 29, 2026T Rhyker Ranallo-Benavidez, Yue Hao, Emilia Volpe, Maryam Jehangir, Noelle Fukushima, Zachary D Stephens, Ogechukwu Mbegbu, Matteo T Ungaro, Rebecca Reiman, Jessica Molnar, et al.time (75 s). Post-sonication QC was performed on a TapeStation HSD5000 (Agilent Technologies, Cat. No. 5067–5592) to verify that fragment sizes were between 550 and 600 bp prior to size selection. Size selection was

(16) Therapeutic targeting of BCL-2 during CART cell production augments potency through non-apoptotic adaptive changesSignal Transduct Target TherApril 27, 2026Nada S. Aboelella, Ryan Park, Erting Tang, Nicholas Asby, Joshua D. Ho, Tony Pan, Sidney Wang, Lishi Xie, Justin P. Kline, Peter A. Riedell, et al.(Invitrogen, Q32851), quality-checked for fragment sizes via High Sensitivity D5000 ScreenTapes (Agilent, 5067–5592), pooled, and sequenced (Illumina, NovaSeq 6000). The raw scRNAseq data were preprocessed using the

(17) Somatic cancer variants enriched in Alzheimer's disease microglia-like cells drive inflammatory and proliferative statesCellApril 21, 2026August Yue Huang, Zinan Zhou, Maya Talukdar, Liz Enyenihi, Michael B. Miller, Brian Chhouk, Ila Rosen, Mengyue Zheng, Minye Zhou, Averill Yang, et al.High Sensitivity D5000 Reagents,Agilent Technologies,5067-5593 High Sensitivity D5000 ScreenTape,Agilent Technologies,5067-5592 "Chromium Next GEM Single Cell ATAC Kit v2, 4 rxn",10X Genomics,1000406 Deposited data,Deposited data,Deposited

(18) Proof-of-concept study for the detection of somatic structural variant driver alterations using HiFi long-read sequencing in a pediatric leukemia cohortnpj Genomic MedicineApril 9, 2026Lisa A. Lansdon, Byunggil Yoo, Ayse Keskus, Irina Pushel, Chengpeng Bi, Tanveer Ahmad, Asher Bryant, Adam Walter, Margaret Gibson, Mary Rindler, et al.concentration. The library size was determined with a High Sensitivity D5000 ScreenTape (Agilent, Cat. No. 5067-5592 and 5067-5593) on the TapeStation platform. Resulting libraries were sequenced with one Revio SMRT Cell

(19) Generation of biologically responsive colon-like intestinal tissue patches from human induced pluripotent stem cells using a rapid co-differentiation platformStem Cell Research & TherapyApril 9, 2026William Dalleywater, Alexander V. Predeus, Batuhan Cakir, Pavel Mazin, Jayakumar Vadakekolathu, Sergio Rutella, Marian L. Meakin, Alison A. Ritchie, Shamir Montazid, Sara Cuevas Ocaña, et al.assessed using the Agilent 4200 TapeStation and Agilent High Sensitivity D5000 ScreenTape Assay (Agilent; 5067–5592 and 5067–5593). Completed sequencing libraries were quantified using the Qubit Fluorometer and the Qubit

(20) Dissecting PGE2-driven inhibition of T cell activation using single-cell multi-omic and inflammatory bowel disease genetic association analysisNature CommunicationsApril 7, 2026Zhongli Xu, Shiyue Tao, Site Feng, Xiangyu Ye, Ting Wang, Haoran Hu, Elisa Heidrich-O’Hare, Wei Chen, Richard H. DuerrSensitivity D5000 assay using HS D5000 Reagents (5067–5593, Agilent Technologies) and HS D5000 ScreenTape (5067–5592, Agilent Technologies), and Gene Expression, ADT, and HTO library average sizes were determined by the

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