StyI-HF from New England Biolabs

Product Specs
ItemStyI-HF
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR3500S
Quantity3000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Cohesin reconstitution and homologous recombination repair of DNA double-strand breaks in late mitosisElifeNovember 11, 2025Jessel Ayra Plasencia, Sara Medina-Suárez, Esperanza Hernández-Carralero, Jonay García-Luis, Lorraine S Symington, Félix Machín2018. Briefly, each gDNA sample was divided into two, and one aliquot was digested with StyI-HF (NEB, R3500S). Then, qPCR reactions were mounted using the PowerUp SYBR Green Master Mix (Thermo Scientific, A25741)

(2) Matrin-3 forms spherical and wormlike assemblies that are modulated by RNA binding and ALS/FTD-associated mutationsMolecular CellOctober 2, 2025Macy L. Sprunger, Min Kyung Shinn, Sabrina K. Talir, Ken Lee, Rohit V. Pappu, Meredith E. Jackrel,New England Biolabs,Cat# R3189S SalI-HF,New England Biolabs,Cat# R0138S StyI-HF,New England Biolabs,Cat# R3500S XhoI,New England Biolabs,Cat# R0146S PfuUltra II Fusion HS DNA Polymerase,Agilent,Cat# 600672 Expi293F

(3) Rapamycin relieves the cataract caused by ablation of Gja8b through stimulating autophagy in zebrafishAutophagyNovember 1, 2021Ping X, Liang J, Shi K, Bao J, Wu J, Yu X, Tang X, Zou J, Shentu X.5ʹ-GAGTGGCAAGATTCGGAAGC-3ʹ) followed by the digestion of StyI restriction enzyme (New England Biolabs, R3500S). In this study, we mated homozygous with WT to obtain heterozygous embryos, and mated homozygous adult

(4) Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling PathwaysCurrent Protocols in Molecular BiologyJune 26, 2020Alejandro Sarrion‐Perdigones, Yezabel Gonzalez, Koen J.T. Venkenno. R3142S), SphI-HF (New England Biolabs, cat. no. R3182S), StyI-HF (New England Biolabs, cat. no. R3500S). Restriction enzyme cutting buffer: CutSmart Buffer (New England Biolabs, cat. no. B7204S). T4 DNA ligase

(5) Development of Transcriptomic Markers for Population Analysis Using Restriction Site Associated RNA Sequencing (RARseq)PLOS ONEAugust 4, 2015Magdy S. Alabady, Willie L. Rogers, Russell L. Malmberg4-base cutter MseI (NEB, R0525S.) For double digestion we used MseI and the 6-base cutter, StyI-HF (NEB, R3500S.) The digestion reaction steps are as following: Normalize the cleaned double stranded cDNA to the appropriate

(6) Combined Micrococcal Nuclease and Exonuclease III Digestion Reveals Precise Positions of the Nucleosome Core/Linker Junctions: Implications for High-Resolution Nucleosome MappingJournal of Molecular BiologyJune 12, 2013Tatiana Nikitina, Difei Wang, Misha Gomberg, Sergei A. Grigoryev, Victor B. ZhurkinWizard SV Gel and PCR Clean-Up System. DNA was further digested with REs AccI and StyI (cat# R0161S, R3500S; New England Biolabs) and treated with 10% SDS for 20 min at 37 °C. Non-denaturing polyacrylamide gel electrophoresis

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StyI-HF

StyI-HF from New England Biolabs

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New England Biolabs

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StyI-HF from New England Biolabs

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