StuI from New England Biolabs

Product Specs
ItemStuI
CompanyNew England Biolabs
Price $70.00 Inquire
Catalog NumberR0187S
Quantity1000 units
Concentration10-100 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

AGG/CCT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) biGMamAct: efficient CRISPR/Cas9-mediated docking of large functional DNA cargoes at the ACTB locusSynthetic BiologyFebruary 13, 2025Martin Pelosse, Marco Marcia(New England Biolabs, cat. no. E2621S) into psLIB TRE digested with StuI (New England Biolabs, cat. no. R0187S) following manufacturer’s recommendations. Briefly, we mixed on ice 0.05 pmol backbone with 0.1 pmol

(2) Efficient sex hormone biosensors in Saccharomyces cerevisiae cells to evaluate human aromatase activity and inhibitionSci RepJanuary 3, 2025Jie Wu, Matthias Bureik, Mario Andrea Marchisioby GENEWIZ (Soochow, China). Acc651 (R0599S), BstXI (R0113S), EcoRV (R0195S), Sacl (R0156S), Stul (R0187S) restriction enzymes, O5 Hot-start polymerase (M0491S), T4 DNA ligase (M0202S), T5 exonuclease (M0663S),

(3) Genetic Tagging and Imaging of Proteins with iFAST in Candida albicansBIO-PROTOCOLOctober 5, 2024Jonas Devos, Patrick Van Dijck, Wouter Van Genechten(New England Biolabs, catalog number: B6004S)Restriction enzymes:StuI (New England Biolabs, catalog number: R0187S)NheI-HF (New England Biolabs, catalog number: R3131S)PstI-HF (New England Biolabs, catalog number: R3140S) Solutions YPD

(4) A large-scale cancer-specific protein–DNA interaction networkLife Science AllianceOctober 1, 2024Yunwei Lu, Anna Berenson, Ryan Lane, Isabelle Guelin, Zhaorong Li, Yilin Chen, Sakshi Shah, Meimei Yin, Luis Fernando Soto-Ugaldi, Ana Fiszbein, et al.linearized using single-cutter restriction enzymes (R0520L, R0146L, R3127S, R0581S, R0193L, R0114S, R0187S, R0519L; New England Biolabs). Table S7. Gene promoter info. The pWM#2 and pWM#3 plasmids for each cancer

(5) Diribonuclease activity eliminates toxic diribonucleotide accumulationCell ReportsSeptember 24, 2024Soo-Kyoung Kim, Mona W. Orr, Husan Turdiev, Conor C. Jenkins, Justin D. Lormand, Tanner M. Myers, Audrey Andy Burnim, Jared.A. Carter, Warren C. Kung, Xiaofang Jiang, et al.E0552S Pierce™BCA Protein Assay kit,Thermo Fisher Scientific,Cat# 23227 StuI,New England Biolabs,Cat# R0187S AluI,New England Biolabs,Cat# R0137S Ni-NTA resin,Thermo Fisher Scientific,Cat# PI88222 Ulp-1 protease,Thermo Fisher Scientific

(6) Protocol for studying topological DNA interactions by purified fission yeast condensinSTAR ProtocolsJune 21, 2024Minzhe Tang, Frank UhlmannK,Thermo Fisher Scientific,Cat#25530049 GelRed nucleic acid gel stain,Biotium,Cat#41003-1 StuI,NEB,Cat#R0187S ScaI-HF,NEB,Cat#R3122S ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models

(7) Combinatorial expression of gamma-protocadherins regulates synaptic connectivity in the mouse neocortexElifeMarch 12, 2024Yi-Jun Zhu, Cai-Yun Deng, Liu Fan, Ya-Qian Wang, Hui Zhou, Hua-Tai Xu(11636090910, Roche), with genomic DNA extracted from mouse tails and digested with BamHI or StuI (R0136, R0187, New England BioLabs). The DNA was separated on a 1% agarose gel and transferred to a positively charged

(8) G-quadruplex in the TMV Genome Regulates Viral Proliferation and Acts as Antiviral Target of Photodynamic TherapyPLOS PathogensDecember 7, 2023Congbao Xie, Xianpeng Zhang, Wenyue Pei, Ju Sun, Hongqi Shang, Zhiyuan Huang, Mengxi Wang, Daozhong Wang, Guiqian Wang, Zhikun Gui, et al.and s3-3. The pH7LIC14−TMV−GFP was digested through restriction enzyme ApaI (R0114, NEB) and StuI (R0187, NEB) to obtain linearized vector. The sequences of TMV at 1058−4845 bp were amplified using primer

(9) Paired yeast one-hybrid assays to detect DNA-binding cooperativity and antagonism across transcription factorsNature CommunicationsOctober 18, 2023Anna Berenson, Ryan Lane, Luis F. Soto-Ugaldi, Mahir Patel, Cosmin Ciausu, Zhaorong Li, Yilin Chen, Sakshi Shah, Clarissa Santoso, Xing Liu, et al.single-cutter restriction enzymes (New England Biolabs R0520L, R0146L, R3127S, R0581S, R0193L, R0114S, R0187S, R0519L). The pWM#2 and pWM#3 plasmids for each promoter were integrated simultaneously into the Y1Has2

(10) Diversity-oriented synthesis encoded by deoxyoligonucleotidesNat CommunAugust 15, 2023Liam Hudson, Jeremy W. Mason, Matthias V. Westphal, Matthieu J. R. Richter, Jonathan R. Thielman, Bruce K. Hua, Christopher J. Gerry, Guoqin Xia, Heather L. Osswald, John M. Knapp, et al.DEL compounds into the supernatant. 20 µL of the elution was restriction digested by 0.1 µL StuI (NEB R0187) in 56.5 µL 1 × SmartCutter buffer (NEB B7204S) per sample (37 °C, 1 h) and cleaned up using the ChargeSwitch PCR Clean-Up Kit

(11) An ultra-sensitive method to detect mutations in human RAS templatesSmall GTPasesDecember 31, 2022Siqi Li, Christopher M. Counter× 10−4 (see exact dilutions in Table 1). MDS assay was performed as described above using StuI (NEB R0187) or HinFI (NEB R0155) as the restriction enzyme, 60°C as exon-specific barcode primer annealing temperature,

(12) A minimally invasive fin scratching protocol for fast genotyping and early selection of zebrafish embryosScientific ReportsDecember 30, 2022Martina Venditti, Catia Pedalino, Marion Rosello, Giulia Fasano, Malo Serafini, Céline Revenu, Filippo Del Bene, Marco Tartaglia, Antonella Lauriline41,42 after the entire 25 µl of the resulting PCR product was digested using 5 units of Stu I (NEB, R0187S) overnight, the presence of a 200 bps and 100 bps denoted a normal genotype (WT) while the presence

(13) Specific viral RNA drives the SARS CoV-2 nucleocapsid to phase separatebioRxivSeptember 14, 2022Christiane Iserman et al.pu57 Nucleocapsid, a kind gift from the Sheahan lab, linearized with NOTI (NEB R3189S) and STUI (NEB R0187S). 100 ng of gel purified DNA was used as a template for in vitro transcription (NEB E2040S) carried

(14) The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosisNature communicationsJuly 16, 2022De Marco Zompit M, Esteban MT, Mooser C, Adam S, Rossi SE, Jeanrenaud A, Leimbacher PA, Fink D, Shorrocks AK, Blackford AN, et al.the indicated amino acids, into pIRESneo2-EGFP-TOPBP1-WT using SbfI-HF® (NEB, R3642S) and StuI (NEB, R0187S) restriction enzymes. pcDNA3.1(+)-N-HA-TOPBP1(740-899) was custom-synthesized by Genscript. pHIV-NAT-T2A

(15) System-Wide Analysis of the GATC-Binding Nucleoid-Associated Protein Gbn and Its Impact on Streptomyces DevelopmentmSystemsJune 28, 2022Chao Du, Joost Willemse, Amanda M. Erkelens, Victor J. Carrion, Remus T. Dame, Gilles P. van Wezel(R0145), EcoRI-HF (R3101), HindIII-HF (R3104), BbsI (R0539), NcoI-HF (R3193), SnaBI (R0130), StuI (R0187), SacI (R0156), and NdeI (R0111). Phusion polymerase (M0532) and T4 DNA ligase (M0202) were also obtained

(16) Machine Learning on DNA-Encoded Library Count Data Using an Uncertainty-Aware Probabilistic Loss FunctionJournal of Chemical Information and ModelingMay 23, 2022Katherine S. Lim, Andrew G. Reidenbach, Bruce K. Hua, Jeremy W. Mason, Christopher J. Gerry, Paul A. Clemons, Connor W. Coleyin B Buffer (100 μL, 10 min, 90 °C). 20 μL of each elution was restriction digested with StuI (NEB R0187) (2 h, 37 °C) and cleaned up using ChargeSwitch PCR Clean-Up Kit (ThermoFisher Scientific CS12000).

(17) Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombinationMolecular CellOctober 1, 2021Jasvinder S. Ahuja, Catherine S. Harvey, David L. Wheeler, Michael Lichten,New England Biolabs,R0175S PstI,New England Biolabs,R0140S PvuII,New England Biolabs,R0151S StuI,New England Biolabs,R0187S BamHI,New England Biolabs,R3136S SacI,New England Biolabs,R0156S EcoRV,New England Biolabs,R0195S Seakem

(18) Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reportersNature ProtocolsSeptember 1, 2021C. G. Piett, T. J. Pecen, D. J. Laverty, Z. D. NagelDigest 100 μg of the pCX-NNX-Δ5GFP plasmid with 150 units of StuI (New England Biolabs, Catalog No.: R0187) in the provided CutSmart buffer in a reaction volume of 500 μL at 37 °C. for 1.5 hr. 66. Confirm

(19) Sulfated glycans engage the Ang-Tie pathway to regulate vascular developmentNature chemical biologyFebruary 1, 2021Griffin ME, Sorum AW, Miller GM, Goddard WA 3rd, Hsieh-Wilson LC.(5’-AGAGCTACAGATGAGAGGGAAG-3’), 1.25 μL of 10 μM reverse primer (5’-CACATTCTTCTCCTCGGTCTTG-3’). To each reaction, 1 μL of StuI (R0187S, New England Biolabs) was added, and the mixture was incubated at 37 °C for 1.5 h. Samples were diluted

(20) Dissecting the Structural Dynamics of the Nuclear Pore ComplexMolecular CellJanuary 1, 2021Zhanna Hakhverdyan et al.hydrochloride,Sigma-Aldrich,Cat.# 26430 3-Indoleacetic acid (auxin),Sigma-Aldrich,Cat.# I2886 StuI,New England Biolabs,Cat.# R0187S NcoI,New England Biolabs,Cat.# R0193S SacI,New England Biolabs,Cat.#R0156S BamHI,New England Biolabs,Cat.#R0136S HindIII,New England Biolabs

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StuI

StuI from New England Biolabs

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StuI from New England Biolabs

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