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StuI
StuI
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StuI
StuI from New England Biolabs
Be the first to write a review!
Citations:
(36)
$70.00 Inquire
from
New England Biolabs for
StuI
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
StuI
Company
New England Biolabs
Price
$70.00 Inquire
Catalog Number
R0187S
Quantity
1000 units
Concentration
10-100 u/µL
Applications
DNA Manipulation
Type
Restriction Enzyme
Temperature
-20 C
Sequence
AGG/CCT
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (36)
(1)
biGMamAct: efficient CRISPR/Cas9-mediated docking of large functional DNA cargoes at the ACTB locus
Synthetic Biology
February 13, 2025
Martin Pelosse, Marco Marcia
(
New England Biolabs
, cat. no.
E2621S
) into psLIB TRE digested with
StuI
(
New England Biolabs
, cat. no.
R0187S
) following manufacturer’s recommendations. Briefly, we mixed on ice 0.05 pmol backbone with 0.1 pmol
(2)
Efficient sex hormone biosensors in Saccharomyces cerevisiae cells to evaluate human aromatase activity and inhibition
Sci Rep
January 3, 2025
Jie Wu, Matthias Bureik, Mario Andrea Marchisio
by GENEWIZ (Soochow, China). Acc651 (R0599S), BstXI (R0113S),
EcoRV
(
R0195S
), Sacl (
R0156S
), Stul (
R0187S
) restriction enzymes, O5 Hot-start polymerase (
M0491S
),
T4 DNA ligase
(
M0202S
),
T5 exonuclease
(
M0663S
),
(3)
Genetic Tagging and Imaging of Proteins with iFAST in
Candida albicans
BIO-PROTOCOL
October 5, 2024
Jonas Devos, Patrick Van Dijck, Wouter Van Genechten
(
New England Biolabs
, catalog number:
B6004S
)Restriction enzymes:
StuI
(
New England Biolabs
, catalog number:
R0187S
)
NheI-HF
(
New England Biolabs
, catalog number:
R3131S
)
PstI-HF
(
New England Biolabs
, catalog number:
R3140S
) Solutions YPD
(4)
A large-scale cancer-specific protein–DNA interaction network
Life Science Alliance
October 1, 2024
Yunwei Lu, Anna Berenson, Ryan Lane, Isabelle Guelin, Zhaorong Li, Yilin Chen, Sakshi Shah, Meimei Yin, Luis Fernando Soto-Ugaldi, Ana Fiszbein,
et al.
linearized using single-cutter restriction enzymes (
R0520L
,
R0146L
,
R3127S
,
R0581S
,
R0193L
,
R0114S
,
R0187S
,
R0519L
;
New England Biolabs
). Table S7. Gene promoter info. The pWM#2 and pWM#3 plasmids for each
cancer
(5)
Diribonuclease activity eliminates toxic diribonucleotide accumulation
Cell Reports
September 24, 2024
Soo-Kyoung Kim, Mona W. Orr, Husan Turdiev, Conor C. Jenkins, Justin D. Lormand, Tanner M. Myers, Audrey Andy Burnim, Jared.A. Carter, Warren C. Kung, Xiaofang Jiang,
et al.
E0552S
Pierce™BCA Protein Assay kit
,
Thermo Fisher Scientific
,Cat#
23227
StuI
,
New England Biolabs
,Cat#
R0187S
AluI
,
New England Biolabs
,Cat#
R0137S
Ni-NTA resin,Thermo Fisher Scientific,Cat# PI88222 Ulp-1 protease,
Thermo Fisher Scientific
(6)
Protocol for studying topological DNA interactions by purified fission yeast condensin
STAR Protocols
June 21, 2024
Minzhe Tang, Frank Uhlmann
K,
Thermo Fisher Scientific
,Cat#
25530049
GelRed nucleic acid gel stain,
Biotium
,Cat#
41003-1
StuI
,
NEB
,Cat#
R0187S
ScaI-HF
,
NEB
,Cat#
R3122S
,,
Experimental models
: Organisms/
strains
,
Experimental models
: Organisms/
strains
,
Experimental models
(7)
Combinatorial expression of gamma-protocadherins regulates synaptic connectivity in the mouse neocortex
Elife
March 12, 2024
Yi-Jun Zhu, Cai-Yun Deng, Liu Fan, Ya-Qian Wang, Hui Zhou, Hua-Tai Xu
(
11636090910
,
Roche
), with genomic DNA extracted from
mouse
tails and digested with
BamHI
or
StuI
(R0136,
R0187
,
New England BioLabs
). The DNA was separated on a 1% agarose gel and transferred to a positively charged
(8)
G-quadruplex in the TMV Genome Regulates Viral Proliferation and Acts as Antiviral Target of Photodynamic Therapy
PLOS Pathogens
December 7, 2023
Congbao Xie, Xianpeng Zhang, Wenyue Pei, Ju Sun, Hongqi Shang, Zhiyuan Huang, Mengxi Wang, Daozhong Wang, Guiqian Wang, Zhikun Gui,
et al.
and s3-3. The pH7LIC14−TMV−GFP was digested through restriction enzyme
ApaI
(
R0114
,
NEB
) and
StuI
(
R0187
,
NEB
) to obtain linearized vector. The sequences of TMV at 1058−4845 bp were amplified using primer
(9)
Paired yeast one-hybrid assays to detect DNA-binding cooperativity and antagonism across transcription factors
Nature Communications
October 18, 2023
Anna Berenson, Ryan Lane, Luis F. Soto-Ugaldi, Mahir Patel, Cosmin Ciausu, Zhaorong Li, Yilin Chen, Sakshi Shah, Clarissa Santoso, Xing Liu,
et al.
single-cutter restriction enzymes (
New England Biolabs
R0520L
,
R0146L
,
R3127S
,
R0581S
,
R0193L
,
R0114S
,
R0187S
,
R0519L
). The pWM#2 and pWM#3 plasmids for each promoter were integrated simultaneously into the Y1Has2
(10)
Diversity-oriented synthesis encoded by deoxyoligonucleotides
Nat Commun
August 15, 2023
Liam Hudson, Jeremy W. Mason, Matthias V. Westphal, Matthieu J. R. Richter, Jonathan R. Thielman, Bruce K. Hua, Christopher J. Gerry, Guoqin Xia, Heather L. Osswald, John M. Knapp,
et al.
DEL compounds into the supernatant. 20 µL of the elution was restriction digested by 0.1 µL
StuI
(
NEB
R0187
) in 56.5 µL 1 × SmartCutter buffer (
NEB
B7204S
) per sample (37 °C, 1 h) and cleaned up using the
ChargeSwitch PCR Clean-Up Kit
(11)
An ultra-sensitive method to detect mutations in human RAS templates
Small GTPases
December 31, 2022
Siqi Li, Christopher M. Counter
× 10−4 (see exact dilutions in Table 1). MDS
assay
was performed as described above using
StuI
(
NEB
R0187
) or
HinFI
(
NEB
R0155
) as the restriction enzyme, 60°C as exon-specific barcode primer annealing temperature,
(12)
A minimally invasive fin scratching protocol for fast genotyping and early selection of zebrafish embryos
Scientific Reports
December 30, 2022
Martina Venditti, Catia Pedalino, Marion Rosello, Giulia Fasano, Malo Serafini, Céline Revenu, Filippo Del Bene, Marco Tartaglia, Antonella Lauri
line41,42 after the entire 25 µl of the resulting
PCR product
was digested using 5 units of Stu I (
NEB
,
R0187S
) overnight, the presence of a 200 bps and 100 bps denoted a normal genotype (WT) while the presence
(13)
Specific viral RNA drives the SARS CoV-2 nucleocapsid to phase separate
bioRxiv
September 14, 2022
Christiane Iserman et al.
pu57 Nucleocapsid, a kind gift from the Sheahan lab, linearized with
NOTI
(
NEB
R3189S
) and
STUI
(
NEB
R0187S
). 100 ng of gel purified DNA was used as a template for
in vitro transcription
(
NEB
E2040S
) carried
(14)
The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosis
Nature communications
July 16, 2022
De Marco Zompit M, Esteban MT, Mooser C, Adam S, Rossi SE, Jeanrenaud A, Leimbacher PA, Fink D, Shorrocks AK, Blackford AN,
et al
.
the indicated amino acids, into pIRESneo2-EGFP-TOPBP1-WT using
SbfI-HF
® (
NEB
,
R3642S
) and
StuI
(
NEB
,
R0187S
) restriction enzymes. pcDNA3.1(+)-N-HA-TOPBP1(740-899) was custom-synthesized by Genscript. pHIV-NAT-T2A
(15)
System-Wide Analysis of the GATC-Binding Nucleoid-Associated Protein Gbn and Its Impact on
Streptomyces
Development
mSystems
June 28, 2022
Chao Du, Joost Willemse, Amanda M. Erkelens, Victor J. Carrion, Remus T. Dame, Gilles P. van Wezel
(R0145),
EcoRI-HF
(
R3101
),
HindIII-HF
(R3104),
BbsI
(
R0539
),
NcoI-HF
(R3193),
SnaBI
(
R0130
),
StuI
(
R0187
),
SacI
(R0156), and
NdeI
(R0111). Phusion polymerase (M0532) and
T4 DNA ligase
(
M0202
) were also obtained
(16)
Machine Learning on DNA-Encoded Library Count Data Using an Uncertainty-Aware Probabilistic Loss Function
Journal of Chemical Information and Modeling
May 23, 2022
Katherine S. Lim, Andrew G. Reidenbach, Bruce K. Hua, Jeremy W. Mason, Christopher J. Gerry, Paul A. Clemons, Connor W. Coley
in B Buffer (100 μL, 10 min, 90 °C). 20 μL of each elution was restriction digested with
StuI
(
NEB
R0187
) (2 h, 37 °C) and cleaned up using
ChargeSwitch PCR Clean-Up Kit
(
ThermoFisher Scientific
CS12000
).
(17)
Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombination
Molecular Cell
October 1, 2021
Jasvinder S. Ahuja, Catherine S. Harvey, David L. Wheeler, Michael Lichten
,
New England Biolabs
,
R0175S
PstI
,
New England Biolabs
,
R0140S
PvuII
,
New England Biolabs
,
R0151S
StuI
,
New England Biolabs
,
R0187S
BamHI
,
New England Biolabs
,
R3136S
SacI
,
New England Biolabs
,
R0156S
EcoRV
,
New England Biolabs
,
R0195S
Seakem
(18)
Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters
Nature Protocols
September 1, 2021
C. G. Piett, T. J. Pecen, D. J. Laverty, Z. D. Nagel
Digest 100 μg of the pCX-NNX-Δ5GFP plasmid with 150 units of
StuI
(
New England Biolabs
, Catalog No.:
R0187
) in the provided
CutSmart buffer
in a reaction volume of 500 μL at 37 °C. for 1.5 hr. 66. Confirm
(19)
Sulfated glycans engage the Ang-Tie pathway to regulate vascular development
Nature chemical biology
February 1, 2021
Griffin ME, Sorum AW, Miller GM, Goddard WA 3rd, Hsieh-Wilson LC.
(5’-AGAGCTACAGATGAGAGGGAAG-3’), 1.25 μL of 10 μM reverse primer (5’-CACATTCTTCTCCTCGGTCTTG-3’). To each reaction, 1 μL of
StuI
(
R0187S
,
New England Biolabs
) was added, and the mixture was incubated at 37 °C for 1.5 h. Samples were diluted
(20)
Dissecting the Structural Dynamics of the Nuclear Pore Complex
Molecular Cell
January 1, 2021
Zhanna Hakhverdyan et al.
hydrochloride,Sigma-Aldrich,Cat.# 26430
3-Indoleacetic acid
(auxin),
Sigma-Aldrich
,Cat.#
I2886
StuI
,
New England Biolabs
,Cat.#
R0187S
NcoI
,
New England Biolabs
,Cat.#
R0193S
SacI
,
New England Biolabs
,Cat.#
R0156S
BamHI
,
New England Biolabs
,Cat.#
R0136S
HindIII
,
New England Biolabs
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StuI from New England Biolabs
Price
$70.00
Inquire
Product Specs
Company Info
Citations
Reviews
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